Objective To evaluate the effect of oxycodone on migration of human colon cancer cells and the role of μ and κ receptors.Methods The human colon cancer HCT116 cells at the logarithmic growth phase were seeded in 24-well or in 6-well plates at a density of 1 × 106 cells/mnl (0.5 ml/well or 2 ml/well,144 wells in total).The cells were divided into 6 groups (n=24 each) using a random number table:control group (group C),1,5 and 10 μmol/L oxycodone groups (group O1,group O2 and group O3),oxycodone plus μ receptor antagonist CTOP group (group O2+CTOP) and oxycodone plus κ receptor antagonist nor-binaltorphimine group (group O2+BNI).The cells were incubated for 24 h with oxycodone 1,5 and 10 μmol/L in O1,O2 and O3 groups,respectively.The cells were incubated for 24 h with 5 μmol/L oxycodone plus 20 μmol/L CTOP and 5 μmol/L oxycodone plus nor-binahorphimin 20 μmol/L in O2+CTOP and O2+BNI groups,respectively.The invaded and migrated cells were counted,and the levels of Ras homolog gene family member A (RhoA),Rho-associated protein kinase 1 (ROCK1),matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected.Results Compared with group C,the number of invaded and migrated cells was gradually decreased,and the levels of RhoA,ROCK1,MMP-2 and MMP9 were gradually decreased in O1,O2 and O3 groups (P＜0.05),and no significant change was found in the parameters mentioned above in group O2+BNI (P＞0.05).Compared with group O2,the number of invaded and migrated cells was significantly increased,and the levels of RhoA,ROCK1,MMP-2 and MMP9 were increased in group O2 + BNI (P＜0.05),and no significant change was found in the parameters mentioned above in group O2+CTOP (P＞0.05).Conclusion Oxyc odone can inhibit the migration of human colon cancer cells,and the mechanism is totally related to inhibition of RhoA/ROCKl signaling pathway activation after activating κ receptors,but not related to μ receptors.

Color instability is the common problem in production of lychee liqueur.The main factors of non-enzymatic browning in lychee liqueur during 36 days storage were investigated by the ultraviolet spectrophotometer and color difference meter.Results were indicated that these factors such as temperature,illumination intensity,reducing sugar concentration,oxygen content kept positive correlation with the degree of browning but negative correlation with the brightness in the process of storage of lychee wine.It was demonstrated low sugar-degree lychee wine could reduce the browning markedly in the sealed bottle place at 4 ℃ when its absorbance reduced only by 0.003 in the 420 nm and brightness decreased by 0.58.

The effects of pulsed electric field(PEF) and pH as well as thermal treatment on the stability of gardenia yellow pigment were investigated in this paper.Results indicated that gardenia yellow pigment exhibited high resistance to intense PEF and thermal treatment.When the applied PEF treatment time was 64 ms,the reservation ratio of gardenia yellow pigment was 97.1%.This was slightly higher by 4.9% than the thermal treatment at 90℃ for 30 min.It was investigated that the gardenia yellow pigment was very sensitive in both strong acid and strong alkaline environments.

The flavonids in the fernery were extracted under different extraction conditions based on the orthogonal experiment design.The results indicated the optimum extracting condition of ultrasonic intensified ethanol extraction method is A2B2C3D3,i.e.,60% ethanol as solvent,ratio of liquid/solid as 1∶60,refluxing time of 60 min.And ICP-AES determination of Ca,Mg,Fe,Zn,Cu,Cr and Ni in fernery was reported in this paper.And the results also showed that the average recovery was 96.3%~104.0% and the RSD was 0.51%~2.3%.

The lustration effect of magnetic seed and flocculant amounts on cane mixed juice was studied based on magnetic seeds flocculation-high gradient magnetic separation(MSF-HGMS).The best amount was determined.The results showed that the optimum clarifying condition was:magnetic seed 50mg/L and the flocculant 3.5mg/L.The survey average result of purified juice was:color 2176.1IU,turbid 253.7MAU,apparent purity 80.0% and filtration velocity 17.64mL/min.

Using submerged technique to produce physiological active substances has been considered one of the focuses in edible mushroom research. With the development of science and technology,more knowledge about the growth of edible mushroom mycelium and secondary metabolites production during submerged culture has been obtained. The research of edible mushroom fermentation conditions,the fermentation kinetics and mycelium morphology are reviewed in this article. The future trend in the edible mushroom liquid fermentation research is also mentioned.