Objective:To explore in-vitro cell toxicity of anti-tumor drug gallic acid(GA)on normal human hepatocyte(LO2).Methods:MTT experiment,flow cytometry,Hoechst-33258 fluorescent staining and electron microscope detection were applied.Results:0,6.25,12.5,25,50,100 and 200μmol/L medication groups presented different degrees of proliferation inhibition after GA acted on 24h,and inhibitory rates were 0,18.2%,25.5%,42.0%,42.5%,51.8% and 60.6% respectively;0,25,50 and 100μmol/L medication groups were detected by flow cytometer,whose apoptosis rates were 1.37%,4.63%,4.64% and 12.53% respectively;fluorescent staining and electron microscope detection indicated that cells manifested morphological changes,nuclear pycnosis and fragmentation in 100μmol/L group.Conclusion:Anti-tumor monomer GA also has the inhibitory effect on the proliferation of cell LO2 and can evoke LO2 apoptosis.

Since the Nobel Prize in medicine was awarded the scholars who found telomere and telomerase in 2009,the researches for telomere and telomerase become a focus again.This paper reviews the influence of telomere length and telomerase activity by the traditional Chinese medicine of anti-aging and anti-tumor,providing new ideas of the research direction for the traditional medicine of anti-aging and anti-tumor.