在相同饲养条件下,用布氏菌病活疫苗(Rev.1株)和布氏菌病活疫苗(S2株)进行羊只的免疫,按照常规免疫剂量分别接种健康绵羊30只,并设空白对照10只,共计70只.免疫前和免疫后14、21、28、49、60、90、120、150 d采血,采用虎红平板凝集试验(RBT)和试管凝集试验(SAT)2种检测方法进行血清抗体检测.结果证实2种疫苗的免疫效果,为指导布鲁氏菌病的防治提供科学依据.

In order to compare the virulence of the applied vaccine strains of brucellosis,which are B.abortus A19,B.melitensis M5 and B.suis S2,three groups of above vaccines with the dose of 1×105 CFU each were inoculated to female Balb/c mice,respectively.The specific bacterica were isolated and identified by PCR from the spleens of each group every 2 weeks post inoculation.The suvival tests shows that A19,M5 and S2 strains could stay in spleen for 14 weeks,more than 16 weeks and 6 weeks post inoculation,respectively.The same dose of bacterias were injected to adult female guinea pigs in groups,respectively,and the amount of ioslated bacteria were calculated for each group of spleens per grame.The amount of containing bacteria for group A19,M5 and S2 were 2.8×104CFU,more than 6.7×105 CFU and 3.8×103CFU.This study shows that in all the three kinds of brucella vaccines,S2 is the weakest strain,while M5 is the strongest one.

<正>人工授精是兔的改良工作中最经济和最科学的一种配种方法。兔的人工授精的能够充分利用优良种公兔,迅速推广良种;防止近亲交配,可减少公兔的饲养数量,公母饲养比例为1∶(80～100),降低饲养成本;提高母兔的受胎率(80%～90%),可减少疾病的传播。

<正>1场址选择兔场应建在地势高燥、背风向阳、有充足干净水源的地方,要求具有透水性好的砂质土壤,要远离交通主干道及屠宰场。