Brucellosis is one of the zoonotic diseases of animal origin that seriously threatens human health.The prevention and control of brucellosis in animals is mainly based on prevention.At present,there are S2,A19 and M5 vaccines for brucellosis in China,and S19 and Rev.1 vaccines in the world,among which M5 and Rev.1 strains of sheep are the least stable.TSA is Tryptone soy agar medium(also known as Tryptone soy agar medium),a commercial medium that is suitable for the growth of Brucella Rev.1 strain.After TSA medium plate with different agar concentration was placed under different conditions(temperature and time),Brucella Rev.1 strain was inoculated.After 96 h culture,colony growth was observed and colony crystal violet staining was performed.Through comparative analysis,it is found that the humidity of TSA medium plate is an important factor affecting the accuracy of variation test results.At the same time,the preparation method of TSA medium for Brucella Rev.1 strain was clarified.

在相同饲养条件下,用布氏菌病活疫苗(Rev.1株)和布氏菌病活疫苗(S2株)进行羊只的免疫,按照常规免疫剂量分别接种健康绵羊30只,并设空白对照10只,共计70只.免疫前和免疫后14、21、28、49、60、90、120、150 d采血,采用虎红平板凝集试验(RBT)和试管凝集试验(SAT)2种检测方法进行血清抗体检测.结果证实2种疫苗的免疫效果,为指导布鲁氏菌病的防治提供科学依据.