A set of backcross population were constructed with Suinong 14 as recurrent parent and wild soybean ZY6 as donor parent. 16 QTLs( 14 QTLs with positive effect,2 QTLs with negative effect) for protein content among 9 linkage groups detected by composite interval mapping( CIM) with Win QTL2. 5. Ten BC3F3lines with high protein content compared with Suinong 14 were screened out,and another 10 lines from BC3F3were used as control( random population). 17 QTLs for protein content on 10 linkage groups were detected with genotype analysis by chi-square on genetic hitchhiking,and all showed positive effect. 7 QTLs were detected by both methods.These materials and markers provided important material foundation and marker information for gene cloning and molecular assisted breeding in high protein content.

定位大豆单株粒重QTL、分析QTL间的上位效应及QTL与环境互作效应,有利于大豆单株粒重遗传机制的深入研究.利用147个F2:14～F2:18 RIL群体,在5年2点多环境下以CIM和MIM方法同时定位大豆单株粒重QTL,检测到17个控制单株粒重的QTL,分别位于D1a、B1、B2、C2、F、G和A1连锁群上,贡献率为6.0％～47.9％；用2种方法同时检测到3个QTL,即qSWPP-DIa-3、qSWPP-F-1和qSWPP-D1a-5,贡献率为6.3％～38.3％；2年以上同时检测到4个QTL,即qSWPP-DIa-1、qSWPP-DIa-2、qSWPP-B1-1和qSWPP-G-1,贡献率为8.1％～47.9％;利用QTLMapper分析QE互作效应和QTL间上位效应,7种环境下的数据联合分析得到1个QE互作QTL和4对上位效应QTL,贡献率和加性效应都较小.在分子标记辅助育种中应该同时考虑主效QTL及各微效QTL之间的互作.

A set of advanced backcross population were constructed with Suinong 14 as recurrent parent and wild soybean ZY6 as donor parent.After comparing with recurrent parent Suinong 14,43 BC3F2 individuals with small seed size were screened out,and another 43 individuals from BC3F2 were used as control(random population).Nine QTLs(quantitative trait loci) on seven linkage groups controlling small seed size were detected with genotype analysis by chi-square on genetic hitchhiking,and these QTLs showed positive effect on small seed size.Advanced backcrossing lines were used for mapping QTL related to small seed size,and could benefit molecular assisted breeding.

[Objective] This study is to map QTLs for lodging-related morphological traits of soybean, aiming at providing basis for breeding lodging-resistant varieties. [Method] To map the QTLs for lodging-related morphological traits, an F216-F218 RIL population containing 147 lines derived from a cross between Charleston as female and Dongnong 594 as male parent were used as experimental materials in this study. Totally 164 SSR primers screened out from amplification on two parents, were employed to amplify the F216-F218 RIL population for constructing a genetic linkage map. The QTLs for nodes on main stem, stem thickness and stem weight of the F216-F218 RIL soybean population were investigated in two eco-environments for three years. [Result] Sixteen QTLs for nodes on main stem were detected in A1, B1, C2, Dla, D2, F, G, H and N linkage groups, respectively; Ten QTLs for stem thickness in A1, B1, C2, Dla, E and G linkage groups, respectively; Fifteen QTLs for stem weight in A1, A2, C2, Dla, Dlb and G linkage groups, respectively. Of these QTLs, five for nodes on main stem, one for stem thickness and six for stem weight could be detected by both CIM and MIM, accounting for 8.6%-27.0%, 9.0%- 11.0%, and 6.0%-39.0% of the general phenotypic variation, respectively. From ex- perimental data of two years, Three QTLs for nodes on main stem and two for stem weight could be detected, accounting for 8.0%-60.2% and 10.0%-23.0% of the general phenotypic variation, respectively; while no QTL for stem thickness was re- peatedly detected during more than two years. [Conclusion] Comparison of the QTLs mapped for nodes on main stem, stem thickness and stem weight indicates that these three morphological traits are closely related with lodging-resistance of soybean.

In order to solve practical problems in soybean breeding,a set of SSR primer were used to screen the polymorphism.Then 23 tested materials were genotyped to reveal their relationship.The results showed that four lines were pseudo-hybrid;one pair of lines were potential sister lines with exactly the same genotypic profiles;12 lines were carrying one parental genetic background and one had both parental backgrounds.Therefore,molecular markers should be useful in detecting the soybean crossing or pedigree relationship.

【Objective】The main purpose of this paper was to map the QTLs related with salt tolerance and low-temperature tolerance at germination stage with backcross introgression lines(ILs),and analyze the genetic overlap between them.These overlapping QTL loci could be used to breed soybean varieties with both salt tolerance and low-temperature tolerance,improve efficiency of stress-tolerance breeding.【Method】A primary backcross introgression lines(ILs) were constructed with Hong-feng11 as recurrent parent and Harosoy as donor parent.Then 48 individuals from BC2F4 introgression populations were obtained after screening in 1.75% NaCl solution,and 40 individuals were screened out after 6℃ low-temperature treatment comparing with the Hong-feng11 at germination stage.The QTL identification of the germination stage was conducted by chi-square test and analysis of variances with the two introgression populations.【Result】There were 22 QTL located with BC2F4 introgression populations after salt stress,and 15 QTL were mapped after low-temperature stress.Seven overlapping QTLs between salt tolerance and low-temperature tolerance were detected on 6 linkage groups at germination stage.【Conclusion】In total,there were 31.81% of salt tolerance and low-temperature tolerance loci existed genetic overlap.

Soybean grows worldwide in many regions under drought stress,so drought tolerance(DT)is a very important trait for the crop.Most researches have focused on the QTL mapping related with drought-tolerance,but there is no research on the genetic overlap in soybean.A primary backcross introgression lines(ILs)were constructed with Hongfeng 11 as recurrent parent and Clark as donor parent.Forty-four individuals from BC1F5 introgression populations were screened out under drought stress comparing with the Hongfeng11 in germination stage,and 46 individuals were obtained under drought stress comparing with the control population in seedling stage.The QTL identification at germination stage and seedling stage was conducted by one-way ANOVA(for single marker analysis,P0.05)with the two introgression populations.Then 10 QTLs were mapped in germination stage,21 QTLs were mapped in seedling stage laced on relative water content(RWC),water holding capacity(WHC),and growth of plant height(GPH).Four QTLs including Satt449,Satt499,Satt440,and Sat_180 in A1,K,I,and H linkage groups were mapped both in germination stage and seedling stage,indicating a partial genetic overlap between these two stages in soybean.The above results would provide a basic for fine mapping and molecular breeding for favorable genes related to DT in soybean.