B cell activating factor (BAFF) belongs to the tumor necrosis factor (TNF) family,which plays a critical role in B cell survival,proliferation,maturation and immunoglobulin secretion.In this study,two primers Os-F and Os-R contained EcoR I and Hind Ⅲ restriction sites were designed to amplify the soluble part of OsBAFF based on the complete OsBAFF sequence in African ostrich in our previous research.The results of SDS-PAGE and western blot analysis confirmed that the soluble fusion protein His-OsBAFF was efficiently expressed in E.coli Rosset (DE3)In vitro,purified OsBAFF was not only able to promote the survival rate of African ostrich bursal lymphocytes,but also able to co-stimulate proliferation of mouse splenic B cells.These findings indicated that OsBAFF played an important role in survival and proliferation of African ostrich bursal lymphocytes,which could provide valuable information for research on the immune system of African ostrich and OsBAFF could serve as a potential immunologic factor for enhancing immunological efficacy in African ostrich and other birds.

硼是广泛分布于自然界的微量元素,具有多种生物学作用.研究表明,硼与某些动物的生长发育和新陈代谢过程密切相关,可能是动物不可或缺的元素.适量的硼可促进动物的生长发育,维持动物的正常生理功能;高剂量的硼则对动物产生毒害作用.目前主要以试验动物为模型,研究硼对动物的营养与毒性作用,从分子水平探讨硼的作用机理.论文结合了硼在动物营养和毒性作用方面的最新研究进展,对硼在提高动物的生长速度、促进动物胚胎发育、改善动物骨代谢等方面的营养作用及发育毒性、生殖毒性和免疫毒性进行综述,旨在为硼的生物学和营养学研究提供参考.

The adjusting effects of Oolong tea polysaccharide(OTPS) on serum lipid in hyperlipoidemia mice and its mechanism were investigated.The hyperlipidmia mice model was established by feeding high lipid diet,and different groups were taken OTPS by intragastric administration for 6 weeks.In present investigation,body weight,thymus weight,spleen weight,liver weight,blood lipid levels,malondialdehyde(MDA) and liver superoxide dismutase(SOD) activities of serum and liver were measured,as well the histopathological changes in tissues of liver were observed.The results showed that the contents of total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),serum and liver MDA,liver index were lower in comparison with the high hyperlipoidemia model group,while thymus index,spleen index,serum and liver SOD activities were somewhat higher,and the liver lesions were restored in some degree.It is indicated that OTPS showed a positive effect on adjusting the serum lipid,improving immunity of body and antioxidation.

采用单因素和正交试验设计对茶叶中β-D-半乳糖苷酶的比活力测定条件进行优化.结果表明,茶叶中β-D-半乳糖苷酶1比活力测定条件为:以pH4.0的柠檬酸-磷酸氢二钠为缓冲液,在60℃下反应7min.对不同茶叶品种、生长季节以及不同嫩度叶片中的β-D-半乳糖苷酶比活力进行分析.结果表明,乌龙茶与绿茶品种中β-D-半乳糖苷酶比活力差异明显,不同季节与不同嫩度茶叶叶片之间也存在较大变化.

The effects of Tengcha flavones and dihydromyricetin(DMY) on hypolipidemic mice were investigated. The results showed, compared with the hyperlipidemia control group, DMY reduced the weights of mice significantly; Tengcha flavones and DMY decreased serum TC、TG、LDL-C、malondiadehyde(MDA) contents and Liver coefficient, increased HDL-C levels and the activities of SOD distinctly. The pathological observation on the liver tissue showed that Tencha flavones and DMY could lighten the denaturation liver cell. As results, Tengcha flavones and DMY could prevent hypolipidemia in mice and protect myocardial cells from oxidation and the liver harmful from of high lipid.