The aim of this study is to investigate the effect of different durations on nuclear maturation in vitro of feline oocytes.Following the in vitro maturation(IVM)in Medium 199 added with FSH and LH,feline oocytes were treated for 24 h,28 h,32 h,36 h and 43 h respectively.Then,the polar body 1(PB1)was observed and oocytes were stained by aceto-orcein solution,and those with PB1 and stayed at MⅡ phase,were considered as nuclear maturation.The rate of nuclear maturation in the 43 h group was significantly higher than the others(P<0.01).The rate of nuclear maturation in the 24 h group was significantly lower than the others(P<0.01),but there was no significant differences of the rate of nuclear maturation among other three groups(P>0.05).In addition,the rate of degeneration in the 43 h group was also significantly higher than the others(P<0.01).Therefore,the optimal nuclear maturation of feline oocytes appeared following the duration from 28 to 36 h by IVM.

The purpose of this study is to establish an efficient way for mouse parthenogenetic activation.Oocytes were recovered from mice at different hours after hCG administration and were denuded of cumulus cells with hyaluronidase.The cleavage and blastocyst rates of mouse oocytes activated with different concentrations of strontium chloride,and different durations of ethanol stimulation with or without 6-dimethylamino-purine,were analyzed.The cleavage rate of oocytes collected at 15-16,18-19,20-21 hours were increased after treatment with 6 mmol/L SrCl_2.The cleavage rate of oocytes collected at 20-21 hour was significantly higher than that of oocytes collected at 18-19 or 20-21 hour(P0.05).The blastocyst rate of oocytes collected at 18-19 hour was the highest among different groups.The cleavage rate of oocytes treated with 6 mmol/L and 10 mmol/L SrCl_2 were 76.4%,83.6%,and the morula rates were 50.0% and 56.3% respectively.The cleavage rate of oocytes treated with 70 ml/L ethanol for 7 min was 77.1%,and the blastocyst rate was also significantly higher than that of the other two groups(P0.05).The combination of 6-DMAP and SrCl_2 or ethanol,improved the extrusion of second polar body,and the diploid parthenogenetic embryonic rate.The average number of parthenogenetic blastocyst cells was significantly lower than that of normal blastocysts(P0.05).The cleavage of mouse parthenogenetic embryos depends on both the oocyte age and activation protocols.The parthenogenetic activation for oocytes collected at 18-19 hour by treatment with 10 mmol/L SrCl_2 or 70 ml/L ethanol combined with 6-DMAP,was the most efficient approach.

To search the better extender,cryoprotectant and thaw temperature for cryopreservate domestic cat epididymal spermatozoa,the domestic cat epididymal spermatozoa was collected and assessed before and after rapid cryopreservation by two different extenders and three different cryoprotectants respectively.Then,they were thawed in warm water bath either at 37 ℃ or 30 ℃.The results showed that extender II was much more effective than another one.As a cryoprotectant,the glycol was also much better than the glycerol and DMSO(P0.05).The post-thawing motility and abnormal sperm rate of the frozen/thawed spermatozoa protected by glycol reached 52.7%±4.9% and 37.3%±4.2% respectively.Its intact acrosome rate was 52.4%±4.1%,but there was no significant difference between the group with glycerol and that with DMSO.It indicated that the glycol and extender II was better for cryopreservate domestic cat epididymal spermatozoa,thawing effect at 37 ℃ was much better than that at 30 ℃.

为摸索繁殖季节和非繁殖季节PMSG和FSH对小尾寒羊超数排卵效果的最佳剂量,本试验分别使用不同剂量的PMSG和FSH两种超排药物对小尾寒羊进行超排处理.结果表明,注射600 U、900 U和1200 U的PMSG,在繁殖季节分别可平均获得3.3、4.3和4.5个胚胎,在非繁殖季节分别可平均获得2.4、2.7和3.1个胚胎;注射3 mg、5 mg和7 mg的FSH,在繁殖季节分别可平均获得6.3、13.0和13.9个胚胎,在非繁殖季节分别可平均获得3.7、7.2和8.0个胚胎.结论:繁殖季节的超排效果优于非繁殖季节,FSH的超排效果优于PMSG,在繁殖季节和非繁殖季节FSH的适宜剂量都是5 mg.

The aim of this study is to determine the ovulation duration of domestic cats after superovulation during their non-breeding seasons.After the treatment with twice injection of 150 IU of pregnant mares'serum,the hot queens were injected with hCG(100 IU per cat)after the treatment with PMSG and allowed to coitus with males in following 24 h.The ovulation assessment was initiated in 24 h,30 h and 36 h after the first copulation by ovariohysterectom,and the embryos were recovered from the oviducts.The results showed that all adults responded with artificially induced estrus during the non-breeding seasons,and the ovulation in most queens was mainly initiated in 30 h after the first copulation.