本文利用苏州市公安系统DNA数据库资源，进行大规模汉族人群的15个基因座的遗传多态性分析。采用AmpFISTR Identiifler试剂盒、AmpFISTR Identiifler Plus试剂盒、AGCU 17+1试剂盒进行扩增，经测序后获得510000名汉族个体15个STR基因座的DNA分型，应用Excel软件计算等位基因的分布频率等群体遗传学数据。在15个STR基因座共检出等位基因344个、基因型1890个。所调查的汉族人群15个基因座具有较好的识别力。

Objective To research the medical tangle from the perspective of forensic judicial authenticator, provide perspectives for preventing and handling medical tangle for related agencies. MethodsThrough autopsy cases of 129 cases of medical tangle were retrospectively analyzed.Results Medical tangle was different in sex, age, clinical departments, involving different cause of death, and has a certain relationship with clinical diagnosis. In recent years, medical tangle has a tendency to increase.ConclusionDiscusses the role of forensic autopsy in medical tangle resolution, suggest to preventing medical tangle and existing problems in the current forensic judicial authentication businesses.

<正>1案例资料吴某,女,28岁。某日在超市与他人因纠纷打架,过程中腹部被对方持购物篮砸中后倒地不起,遂被送往医院急救。查体:神清,痛苦貌,腹部皮肤未见损伤,肌抵抗,压痛(+),反跳痛(+),右腹部触及肿块,腹腔穿刺抽出少许红褐色液体,其穿刺液中白细胞(+++)。X线检查:膈下多发性游离气体。以"消化道穿孔、急性腹膜炎"收住入院。即日行"剖腹探查、小肠部分切除术"。术中见:回肠末段见一处破

1 案例 某男,18岁,学生,在寝室与同学发生争执时,被踢中腹部当即倒地,经送医院抢救无效死亡.尸表检查:成年男性尸体,尸长167.0 cm,发育正常,营养良好.尸斑位于身体背侧未受压处,呈暗红色.头皮无损伤,黑发,顶部发长5.0cm.双眼角膜轻度混浊.左眼下睑结膜见点状出血,右眼睑、球结膜苍白.