目前国内保健品一般采用层流洁净室内分装后直接为成品,不再进行消毒灭菌.层流洁净室一般代价较为昂贵,建立的成本较高,使用的成本也偏高.

<正>2008年9月初爆发的三聚氰胺奶粉事件,让人们在对受害儿童深感痛心之余,无不对我国食品安全监管存在的问题提出诸多质疑和反思。有人认为我国社会诚信缺失,经营者见利

Objective:To investigate the effect of homocysteine (HCY) in promoting senescence of cultured endothelial cells (EC) and the underlying mechanism. Methods: Bovine aortic endothelial cells were isolated by enzymatic digestion method from new born calf and were randomly divided into 4 groups. Cells in control group were not treated with HCY and those in the other 3 groups were treated with HCY with the final concentrations being 0.1, 0.5, and 1.0 mmol/L. The cultured cells were observed morphologically and stained with β-Gal; the cell cycle was examined with flow cytometric method (FCM) and the telomere length was analyzed by Southern blotting. Meanwhile, the contents of nitric oxide(NO), endothelin (ET), MDA and the activity of GSH-Px were determined in the supernatants. Results: Compared with control group, the 3 HCY groups had more β-Gal positive cells and shorter telomere length. Cells of G0/G1 phase in the 3 HCY groups were significantly increased than those of control group (P0.05) and the cells of S-phase were significantly decreased (P0.01); the contents of NO in supernatants of 3 HCY groups were significantly lower than that of control group(P0.05) and the contents of ET, Ox-LDL were markedly higher than that of control group(P0.05); the contents of MDA and the activity of GSH-Px were similar in all groups. Conclusion: It is indicated that homocysteine may accelerate senescence in endothelial cells, which is not related to oxygenation and reduction.

午餐是一天中摄入营养最多的一餐,基本占一天总能量的40%左右.合理安排午餐,既做到合理营养,又符合学生的口味,且安全卫生十分重要.目前,上海市宝山区65所中学中设有学生食堂的共64所,其中由宝山教育配膳服务社(宝教)负责集体配送午餐原料的有20所,由宝钢餐饮中心(宝钢)负责配送午餐原料的有9所,自行采买(自行)的有20所,我们对这3种供给模式的午餐营养成分及安全性进行了分析比较.