Objective:To screen the differentially expressed genes (DEG) related to inflammatory response associated with the prognosis of colon cancer based on the bioinformatics approach, and to construct and validate a prognostic model for colon cancer.Methods:RNA sequencing and clinical data of 472 colon cancer patients and normal colon tissues of 41 healthy people were retrieved from the Cancer Genome Atlas (TCGA) database. Gene expression related to prognosis of colon cancer and clinical data were retrieved from the International Cancer Genome Consortium (ICGC) database. The retrieval time was all from the establishment of library to November 2022. A total of 200 genes associated with inflammatory response obtained from the Gene Set Enrichment Analysis (GSEA) database were compared with the RNA sequencing gene dataset of colon cancer and normal colon tissues obtained from the TCGA database, and then DEG associated with inflammatory response were obtained. The prognosis-related DEG in the TCGA database were analyzed by using Cox proportional risk model, and the inflammatory response-related DEG were intersected with the prognosis-related DEG to obtain the prognosis-related inflammatory response-related DEG. The prognostic model of colon cancer was constructed by using LASSO Cox regression. Risk scores were calculated, and colon cancer patients in the TCGA database were divided into two groups of low risk (< the median value) and high risk (≥the median value) according to the median value of risk scores. Principal component analysis (PCA) was performed on patients in both groups, and survival analysis was performed by using Kaplan-Meier method. The efficacy of risk score in predicting the overall survival (OS) of colon cancer patients in the TCGA database was analyzed based on the R software timeROC program package. Clinical data from the ICGC database were applied to externally validate the constructed prognostic model, and patients with colon cancer in the ICGC database were classified into high and low risk groups based on the median risk score of patients with colon cancer in the TCGA database. By using R software, single-sample gene set enrichment analysis (ssGESA), immunophenotyping difference analysis, immune microenvironment correlation analysis, and immune checkpoint gene difference analysis of immune cells and immune function were performed for prognosis-related inflammation response-related DEG in the TCGA database.Results:A total of 60 inflammatory response-related DEG and 12 prognosis-related DEG were obtained; and 6 prognosis-related inflammatory response-related DEG (CCL24, GP1BA, SLC4A4, SRI, SPHK1, TIMP1) were obtained by taking the intersection set. LASSO Cox regression analysis showed that a prognostic model for colon cancer was constructed based on 6 prognosis-related inflammatory response-related DEG, and the risk score was calculated as = -0.113×CCL24+0.568×GP1BA+ (-0.375)×SLC4A4+(-0.051)×SRI+0.287×SPHK1+0.345×TIMP1. PCA results showed that patients with colon cancer could be better classified into 2 clusters. The OS in the high-risk group was worse than that in the low-risk group in the TCGA database ( P < 0.001); the area of the curve (AUC) of the prognostic risk score for predicting the OS rates of 1-year, 3-year, 5-year was 0.701, 0.685, and 0.675, respectively. The OS of the low-risk group was better than that of the high-risk group in the ICGC database; AUC of the prognostic risk score for predicting the OS rates of 1-year, 2-year, 3-year was 0.760, 0.788, and 0.743, respectively. ssGSEA analysis showed that the level of immune cell infiltration in the high-risk group in the TCGA database was high, especially the scores of activated dendritic cells, macrophages, neutrophils, plasmacytoid dendritic cells, T helper cells, and follicular helper T cells in the high-risk group were higher than those in the low-risk group, while the score of helper T cells 2 (Th2) in the high-risk group was lower compared with that in the low-risk group (all P < 0.05); in terms of immune function, the high-risk group had higher scores of antigen-presenting cell (APC) co-inhibition, APC co-stimulation, immune checkpoint, human leukocyte antigen (HLA), promotion of inflammation, parainflammation, T-cell stimulation, type Ⅰ interferon (IFN) response, and type ⅡIFN response scores compared with those in the low-risk group (all P < 0.05). The results of immunophenotyping analysis showed that IFN-γ-dominant type (C2) had the highest inflammatory response score, and the differences were statistically significant when compared with trauma healing type (C1) and inflammatory response type (C3), respectively (all P < 0.05). Immune microenvironment stromal cells and immune cells were all positively correlated with prognostic risk scores ( r values were 0.35 and 0.21, respectively, both P < 0.01). The results of immune checkpoint difference analysis showed there was a statistically significant difference in programmed-death receptor ligand 1 (PD-L1) expression level between high-risk group and low-risk group ( P = 0.002), and PD-L1 expression level was positively correlated with prognostic risk score ( r = 0.23, P < 0.01). Conclusions:Inflammatory response-related genes may play an important role in tumor immunity of colon cancer and can be used in the prognostic analysis and immunotherapy of colon cancer patients.

食品塑料包装安全性现状 食品包装被称为特殊的食品添加剂,它是现代食品工业的最后一道工序,在一定程度上,食品包装已成为食品不可分割的重要组成部分.随着人们生活水平的不断提高和对健康消费的日益重视,食品包装材料已受到全社会的普遍重视,食品用塑料容器、软塑包装材料导致的食品安全问题逐渐引起社会各界的关注.

Objective To investigate the effects of aloe-emodin(AE)on splenic T cell subsets in mice with colitis induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).Methods Forty BALB/C mice were randomly divided into normal control group,model group,AE group and sulfasalazine(SASP)group.Mice in the last three groups were given enema of TNBS to induce colitis,and 8 hours later were given intragastrical administration of normal saline,AE(150 mg.kg-1)or SASP(260 mg.kg-1)respectively once a day for 6 continuous days.Everyday,the disease activity index(DAI) was scored.At the end of medication,colonic tissues damage index(TDI) was evaluated,and the percentages of CD4+/CD8+T cells as well as Th1,Th2,Tc1 and Tc2 cells in mice spleen were analyzed by flow cytometry(FCM).Results DAI and TDI scores in AE group were significantly lower than that of the model group(P < 0.05 or P < 0.01).Meanwhile,the results of FCM analysis showed that the percentage of CD4+T cells,CD4+/CD8+ ratio,spleen index,the percentages of Th1 and Tc1 cells,and the ratio of Th1/ Th2,Tc1/Tc2 were markedly decreased(P < 0.05~P < 0.001),while the percentages of CD8+ T cells and Th2 cells were increased obviously(P < 0.05~P < 0.001) in the spleen of AE group.Conclusion AE treatment can significantly relieve the inflammatory pathological injury in TNBS-induced colitis of mice,which might be associated with the regulation of CD4+/CD8+T cells balance,inhibition of Th1 /Tc1 differentiation,and induction of Th2 type immunity.

Objective To investigate the mechanism of emodin in inhibiting of CT26.WT cell line.Methods The proliferation and migration capacities of CT26.WT cells were assessed by MTT method and scratch assay,respectively;while the expression of CCR4 and Foxp3 was assessed by flow cytometry.Results Significant inhibition of cell proliferation and migration of CT26.WT was observed by Emodin in a concentration-dependent manner(P < 0.05);so was the down-regulation of Foxp3 and CCR4(P < 0.01).Conclusion Emodin inhibits the CT26.WT cell proliferation and migration,which is probably via the down-regulation of CCR4 and Foxp3.

Objective To compare the changes of CD4+/CD8+ T cell subsets and intracellular cytokines of Th1/Th2,Tc1/Tc2 cells in different tissues of contact hypersensitivity(CHS) mice.Methods BALB/c mice were sensitized and challenged with dinitrofluorobenzene(DNFB) to induce CHS model.Trichromatism flow cytometry was adopted to detect the changes of CD4+ and CD8+ T cell subsets in the spleen(SP),retroauricular lymph nodes(RLN),intestinal Peyer's patches(PPs) and mesenteric lymph nodes(MLN) as well as the expression of intracellular cytokines of interferon gamma(IFN-γ) and interleukin 4(IL-4) cells.The changes of Th1/Th2 and Tc1/Tc2 ratio in different tissues of CHS mice were also observed.Results Compared to the normal group,CD4/CD8 ratio decreased significantly in RLN and SP of CHS mice(P<0.01).Tc1 and Th1 cells were dominated in RLN of CHS mice,Tc1 and Th2 cells dominated in SP,Tc1 cells percentage and CD4/CD8 ratio significantly increased in PPs(P<0.05).There was no significant difference in the indexes of MLN.Conclusion The murine contact hypersensitivity is mainly mediated by Tc1(CD8+IFN-γ+) effector cells,and the intestinal mucosal immunity and system immunity in DNFB-induced CHS mice are dominated by Th1 cells.

Objective To study the protective effect of polysaccharides of Sijunzi Decoction(SD) on the intestinal mucosa and systematic immune injury of tumor-bearing mice after chemotherapy.Methods S180 cells were inocubated into BALB/c mice to induce solid tumor,and then the model mice were randomized into model group,cyclophosphamide group(intravenous injection of cyclophosphamide 20 mg/kg),and SD polysaccharides group(oral use of SD polysaccharides 20 mg/kg,and intravenous injection of cyclophosphamide 20 mg/kg).Normal control group was also set up.Effect of SD polysaccharides on the whole immunity was investigated through the observation of thymus index,spleen index,peripheral blood lymphocyte count,tumor suppression rate and interleukin 2(IL-2) level in splenic lymphocytes of tumor-bearing mice.Moreover,intestinal sIgA level and IL-2 level in the mesenteric lymph nodes(MLNs)were detected for the evaluation of the effect on intestinal mucosal immunity in tumor-bearing mice.Results SD polysaccharides obviously promoted the secretion of IL-2 level in MLNs and intestinal sIgA level,up-regulated the decreased thymus index,spleen index and peripheral blood lymphocyte number,and promoted the secretion of IL-2 in splenic lymphocytes in tumor-bearing mice after chemotherapy(P0.05 or P0.01).Conclusion SD polysaccharides can relieve immune injury induced by cyclophosphamide through regulating the intestinal mucosal immunity as well as the systematic immunity in tumor-bearing mice after chemotherapy.

目的 确定福州半野生养心草的最佳采收期.方法 采用分光光度法,以没食子酸和芦丁为对照品,对进入开花期至枯萎期养心草药材中的总酚酸和总黄酮含量进行定期测定.结果 养心草药材中总酚酸和总黄酮的含量在开花期后一段时间开始迅速下降.结论 6月中旬至7月中旬为养心草的最佳采收期.

Objective To explore the effects of polysaccharides of Sijunzi decoction(SJZPS)on lymphocyte in spleen,Peyer’s patchs(PPs) and mesenteric lymph nodes(MLNs) in tumor-bearing mice tread with chemotherapy.Methods BALB/c mice were randomly divided into four groups,control group,tumor model group,cyclophosphamide(CY) chemotherapy group and SJZPS treatmen(t CY+SJZPS)group.CY group and CY+SJZPS group daily intraperitoneal injection administration of cyclophosphamide(20 mg.kg-)1,CY+SJZPS group daily oral administration of SJZPS(1 g.kg-1)at the same time.The proportion and activation level of(T,B)lymphocyte,subsets of T lymphocyte isolated from spleen,PPs and MLNs were detected by flow cytometer.Results In CY+SJZPS group,the intestine mucosal immunity injury induced by cyclophosphamide was improved by increasing the proportion and activation level of B lymphocyte in PPs,decreasing the ratio of CD4+/CD8+ in MLNs,and the systematic immunity was improved by increasing the proportion of B cell and the activation level of T、B cell,and decreasing the ratio of CD4+/CD8+ of spleen lymphocyte in tumor-bearing mice tread with chemotherapy.Conclusion Polysaccharides of Sijunzi decoction can effectively antagonize the injury on intestine mucosal immunity induced by cyclophosphamide in tumor-bearing mice,and can improve the systematic immunity.

In order to determine Pb and Cd in fruit juice,fruit juice was digested and treated with microwave to detect them by GFA in this paper.We selected the best sample processing method and the excellent measurement condition.The results showed that the optimum digestion conditions are as follows: digestion conditions: HNO3-H2O2(7∶1,V/V),180 ℃,1 200 W,8 min.The RSD of this method is less than 5.0%.The limit of this method is: Pb 6.0 μg/L,Cd 1.0 μg/L;The recovery of the method is: Pb 78.7%~102.4%,Cd 97.8%~108.7%.The detection limit was also decreased by increasing the sampling amount by pre-digestion at 140 ℃,20 min.The results show that this method is simple,accurate and fast.

The proficiency-testing scheme concerning cobalt determination extracted from glazed ceramic surfaces involved 16 Chinese laboratories as participants,and has been well implemented.All the test materials passed the statistical homogeneity tests,and the performance of participants was based on z-scores.As a result,the majority of participants obtained satisfactory z-scores and laboratories that need to revise their procedures were singled out.Overall,about 12.5% of all results submitted in this PT round were unsatisfactory in terms of z-scores(2 out of 16 total data sets submitted).