目的 探索早期综合发展在农村的可行性方案,评价实施效果.方法 以自然村为单位,将80例新生儿作为实验组,开展早期综合发展服务;另选93例新生儿作为对照组,仅开展农村系统保健.在出生时、6月龄、12月龄常规方法测量身长、体质量.在6月龄、12月龄用儿童智能发育筛查测验(DST)量表评定发育商、智力指数.结果 实验组与对照组身长在出生和6月龄时差异无统计学意义;12月龄时分别为(76.3±1.63)cm、(75.7±1.52)cm,实验组大于对照组差异有统计学意义(t=2.50,P＜0.05).体质量、发育商、智力指数3项指标在出生时差异无统计学意义,在6月龄、12月龄时实验组均大于对照组,差异均有统计学意义.结论 此次在农村儿童中实施早期综合发展服务,促进了婴儿体格、心理发展.

Aim:To observe the inhibitory effects of 10 kinds of traditional Chinese medicine(TCM) extracts on α-glucosidase activity.Methods:Using maltose as substrate,the inhibitory effect of 10 kinds of TCM extracts on α-glucosidase activity was observed.Acarbose was used as a positive control.The reaction solution was taken for the determination of glucose by glucose oxidase method.The absorbance of reaction solution was detected,and the inhibition rate of 10 kinds of TCM extracts on α-glucosidase activity was calculated.Three TCM extracts with higher inhibitory effect were further observed at different mass concentrations.Results:All the extracts showed inhibitory effect on α-glucosidase activity.Among them,Fructose Canarii,Radix Sanguisorbae and Radix Salviae Miltiorrhizae had higher inhibition rate.The inhibition rate of these 3 extracts increased along with the increase(10~1 000 g/L) of the mass concentration(F=71.963,78.426 and 26.246,P0.001).Conclusion:The extracts of Fructose Canarii,Radix Sanguisorbae and Radix Salviae Miltiorrhizae have relatively higher inhibitory effect on α-glucosidase activity.

AIM: To observe the effects of medicinal indianmulberry root on apoptosis of cardiac myocytes in overtrained rats. METHODS: The experiment was conducted at the Department of Pharmacology, Medical College of Zhengzhou University from September 2003 to July 2004. Forty-five SD rats were selected and randomly divided into control group, overtraining group, and overtraining and medicinal indianmulberry root group with 15 rats in each group. The rats in the overtraining group and overtraining and medicinal indianmulberry root group were all treated with progressively the load swimming training to build training models, six days each week for 7 weeks. The rats in the overtraining and medicinal indianmulberry root group were perfused with 4.5 mg/kg per day medicinal indianmulberry root one hour before training. Those in the control group were fed for 7 weeks routinely without intervention. Rat hearts in the 3 groups were gained after 7 weeks, and then cut into piece and digested with centrifuge to make cell smear. The apoptosis rate of the rat cardiac muscle cells was detected by in situ end-labeling (ISEL) technology. The cardiac muscle was made into saline liquid for 10% by homogenization. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) were tested. RESULTS: All the rats in every group were involved in the result analysis, without drop-out rats. ① Effects of medicinal indianmulberry root on apoptosis rate of myocardial cells: The ISEL showed the positive nucleus appeared in myocardial cells of control group by chance. Many positive marks appeared in myocardial cells of overtraining group. The positive labeling appeared diffusedly in the overtraining and medicinal indianmulberry root group, and the positive apoptosis rate was lower significantly than that in the overtraining group (6.80±1.99) %,(11.40 ±2.32) %,(P < 0 01). ② Effects of medicinal indianmulberry root on contents of SOD and MDA of myocardial cells: The activity of SOD of myocardial cells in the overtraining group was lower obviously than that in the control group and the overtraining and medicinal indianmulberry root group (2.61±0.21) mkat/g,(5.10±0.21,3.89±0.15) mkat/g,(P < 0.01). The content of MDA was higher markedly than that in the control group and the overtraining and medicinal indianmulberry root group (3.67±0.17) μmol/g,1.54±0.11,2.09±0.16) μmol/g,(P < 0.01). There was no distinctly difference between the overtraining and medicinal indianmulberry root group and the control group. CONCLUSION: The overtraining lead to the apoptosis ratio increasing significantly in myocardial cells of rats. The medicinal indianmulberry root can inhibit the over-apoptosis of myocardial cells by its anti-oxidation and protect myocardial cells.

目的观察应用运动疗法后2型糖尿病患者血糖、血脂水平的变化情况。方法选择2004-02/06在郑州大学第一附属医院内分泌科就诊的2型糖尿病患者40例,均自愿参加观察。对40例患者进行运动治疗,包括热身活动、运动锻炼和放松运动3个部分。根据患者的年龄、体质量、有无并发症来决定其运动强度、运动方式。运动时要遵循循序渐进、持之以恒的原则,密切观察患者运动时的反应,运动频率5～7次/周,每周至少3次。分别于运动前和运动后第2,4,6,8周,检测患者空腹血糖、总胆固醇和三酰甘油水平。结果纳入2型糖尿病患者40例,全部进入结果分析,无脱落。①运动前及运动后患者血糖水平的比较运动后第2周患者的空腹血糖和餐后2h血糖水平均显著低于运动前[（6.35±0.48,7.01±0.67）m m ol/L,（6.40±0.53,11.98±1.24）m mol/L（F=2.76,P＜0.01）],运动后第4周患者的空腹血糖水平显著低于治疗后第2周[（5.72±0.39）m mol/L（F=6.58,P＜0.05）]。②运动前及运动后患者血脂水平的比较运动后第2周患者的总胆固醇和三酰甘油水平均显著低于运动前[（5.01±1.02）,（6.63±1.52）m m ol/L;（1.48±0.32）,（1.82±0.48）m m ol/L（F=3.21,P＜0.01）],运动后第4周患者的总胆固醇水平显著低于运动后第2周[（4.82±0.72）m m ol/L（F=2.25,P＜0.05）]。结论运动疗法可以明显改善糖尿病患者的血糖、血脂水平,是糖尿病患者长期综合治疗方法之一,长期使用效果更佳。

目的:通过建立力竭游泳训练模型,观察巴戟天对运动训练大鼠骨骼肌自由基代谢及运动能力的影响.方法:实验于2004-04/12在郑州大学医学院药理教研室完成.选择雄性SD大鼠24只,随机分为安静对照组,过度训练组,过度训练+巴戟天组,每组8只.过度训练组、过度训练+巴戟天组大鼠均采用递增负荷游泳训练建立力竭游泳训练模型,每周训练6 d,共7周.过度训练+巴戟天组大鼠同时于训练前1 h灌服巴戟天4.5 mg/(kg·d).安静对照组只进行常规游泳训练,30 min/d,每周训练6 d,共训练7周.7周后将各组大鼠处死,立即分离骨骼肌,用预冷的生理盐水洗净血污,滤纸吸干,按1:9(W/V)加入低温磷酸盐缓冲液(pH7.2)匀浆,离心(5000 r/min,25 min,0～4℃),取上清液,在1～4℃的冰箱保存,以测定超氧化物歧化酶和谷胱甘肽过氧化物酶活性、Ca2+-ATP酶和Na+-K+-ATP酶活性.组间均数比较采用t检验.结果:各组大鼠均进入结果分析,无脱落.过度训练组大鼠力竭时间明显低于安静对照组和过度训练+巴戟天组(P＜0.05);经过7周训练,过度训练组大鼠肌细胞超氧化物歧化酶和谷胱甘肽过氧化物酶活性、Ca2+-ATP酶和Na+-K+-ATP酶活性显著低于安静对照组和过度训练+巴戟天组(P＜0.05),安静对照组与过度训练+巴戟天组无显著性差异.结论:巴戟天可显著提高运动大鼠抗自由基氧化的功能,使大鼠运动能力明显增强.