Following the publication of the above paper, a concerned reader drew to the Editor's attention that, for the RT‑qPCR experiments shown in Fig. 2A on p. 240, data which were intended to show the results of experiments performed in two different cell lines (MCF7 and MDA‑MD‑231) appeared to have been duplicated, with some evidence of stretching of the bands. In addition, it was shown that, in Fig. 5A and C on p. 242, data which were also intended to show the results of RT‑qPCR and western blot analysis experiments performed in the same two cell lines appeared to have been duplicated; moreover, GAPDH data in Fig. 2C also appeared to have been included in Figs. 5C and 6C, but with the data bands shown in different orientations, and probable rearrangement of bands had occurred with the cyclin D1 / MMP‑9 data in Fig. 5C compared with Fig. 6C. Owing to the large number of duplications of data that have been identified in this paper, the Editor of Oncology Reports has decided that it should be retracted from the Journal on account of a lack of confidence in the presented data. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [Oncology Reports 26: 237‑245, 2011; DOI: 10.3892/or.2011.1271].

Objective:To investigate the application value of needle aspiration cell block technique in preoperative molecular classification of breast cancer.Methods:Seventy-five patients with breast cancer from March 2020 to July 2022 were selected as research objects. All patients were examined by needle aspiration cell block technique before surgery，and the application value of needle aspiration cell block technique in preoperative molecular typing of breast cancer was evaluated based on postoperative histopathological and immunohistochemical results. SPSS20.0 software was used to process the data. The measurement data conforming to normal distribution was represented by（xˉ±s），and the count data was represented by［n（%）］，using χ2 test. P<0.05 indicated statistically significant difference.Results:75 female patients diagnosed with highly suspicious breast cancer and breast cancer by needle aspiration cell block technique before surgery were all confirmed to be breast cancer by postoperative histopathological examination. The accuracy of needle aspiration cell block technique for breast cancer diagnosis was 100%. The comparison of preoperative needle aspiration and postoperative histopathological immunohistochemical results showed that，The coincidence rates of progesterone receptor（PR），estrogen receptor（ER），human epidermal growth factor receptor 2（Her-2）and nuclear proliferation antigen（Ki-67）were 97.3%（73/75），98.7%（74/75），97.3%（73/75）and 94.7%（71/75），respectively. There was no significant difference in the expression of each molecular marker between the breast cancer cell block and the corresponding tissue（P>0.05），and the total coincidence rate of preoperative needle aspiration cell block technique and postoperative histopathological molecular typing reached 96.0%.Conclusion:Needle aspiration cell block technique is an accurate and easy method in preoperative diagnosis of breast cancer. It not only improves the accuracy of breast cancer diagnosis，further defines the molecular classification of breast cancer，and helps clinicians to grasp more accurate biological characteristics of breast cancer before surgery.

目的 探讨"互联网+"志愿者服务模式对乳腺癌术后患者情绪及生活质量影响.方法 选取2019年6月至12月首都医科大学附属北京友谊医院(以下简称"我院")行根治性乳癌手术的住院患者80例作为对照组,接受面对面志愿者服务方案;选取2020年1月至10月在我院行根治性乳癌手术的住院患者80例作为试验组,接受"互联网+"志愿者服务方案.采用焦虑自评量表(SAS)、抑郁自评量表(SDS)及中文版癌症患者生活质量测定量表(QLQ-C30),比较两组术前及术后4个月焦虑及抑郁水平,评估患者生活质量.结果 术后4个月后,两组SAS、SDS评分均低于术前,且实验组SDS评分低于对照组(P<0.05);术后4个月,除了对照组认知功能评分与术前比较,差异无统计学意义(P>0.05),两组躯体功能评分均低于术前,情绪功能、认知功能、社会功能、角色功能及总体生活质量评分高于术前,且试验组躯体功能、认知功能、角色功能及总体生活质量评分高于对照组(P<0.05).结论 "互联网+"乳腺癌术后志愿者服务模式在现阶段优于面对面志愿者服务模式,对于患者抑郁状态及生活质量的改善有显著作用,能让更多乳腺癌患者获益.

Introduction Breast cancer is the most prevalent cancer and the leading cause of cancer-related death in women. Conventional open mastectomy (C-OM) is one of the most common procedures for breast cancer, which involves the removal of the nipple-areola complex and a large proportion of the breast skin, leading to poor cosmetic effect and restriction of upper extremity function. Single-port insufflation endoscopic nipple-sparing mastectomy (SIE-NSM) could conceal the incision along the wrinkles in the axilla, preserve all the breast skin and nipple-areola complex and provide a better cosmetic outcome and quality of life. This trial aims to investigate the oncological safety between SIE-NSM and C-OM in early breast cancer patients. Methods and analysis This is a single centre, non-blinded, randomised controlled trial (RCT) and will be conducted at Beijing Friendship Hospital. Patients will be enrolled in the inpatient ward. Breast surgeons will notify patients who meet the inclusion and exclusion criteria with the instruction of this RCT. Patients will be randomly assigned to C-OM or SIE-NSM with a 3:1 allocation as per a computer-generated randomisation schedule. Patients will be followed-up for 12 months for analysing surgical outcomes. The primary outcome is the local recurrence rate at a 12-month follow-up. The secondary outcome is the distant metastasis rate, cosmetic satisfaction score and psychosocial well-being score after a 12-month follow-up. To ensure the accuracy of the cosmetic satisfaction score and psychosocial well-being score, the standard scale, Breast-Q score, will be applied. Ethics and dissemination This study will be conducted according to the medical ethics committee of the Beijing Friendship Hospital and according to the principles of the Declaration of Helsinki. All patients will receive clear instruction of their disease and treatment plan. Informed consent will be obtained from all patients when they agree to comply with our research plan. The results will be disseminated at academic presentations and publications in peer-reviewed journals. The raw data will be confidentially stored in our electronic data capture database. Data will not be shared unless an appropriate data request is submitted after the trial completion and peer-review journal publication. Trial registration number NCT04461847.

BackgroundWe sought to explore the impact of changing treatment strategy based on circulating tumor cells (CTC) on postoperative survival of breast cancer.MethodsWe retrospectively analyzed records of patients who underwent surgery for early-stage breast cancer at Beijing Friendship Hospital from January 2016 to January 2018 and regularly underwent CTC examination after surgery. During the regular examination and CTC monitoring, the patients with positive CTC results and without distant metastasis had their treatment regimen changed.ResultsOf 109 patients who received CTC examination regularly after surgery, 61 (56.0%) were CTC-positive during postoperative follow-up, including 33 ER or PR-positive, and 28 ER and PR-negative patients. Of the 33 ER or PR-positive patients, 20 changed endocrine therapy drugs. Compared with those without replacement, those with changed endocrine therapy strategy had higher CTC clearance rates (90.0% vs. 53.8%, p=0.04) and significantly lower CTC-positive values (1.70 ± 1.72 vs. 0.62 ± 0.65, p = 0.04). Among the 28 patients who were CTC positive and ER and PR-negative, 11 used capecitabine. Compared with non-users, the capecitabine users had higher CTC clearance rates (100.0% vs. 52.9%, p=0.01) and more significant decrease in CTC-positive values (2.09 ± 1.14 vs. 0.82 ± 1.67, p=0.04). Disease-free survival (DFS) at 1, 3, and 5 years was significantly longer in those who changed treatment than in those who did not (respectively, 96.6% vs. 89.6%, 92.8% vs. 56.9%, 69.0% vs. 47.8%, p<0.01). By changing the treatment strategy, CTC-positive patients achieved DFS that was not significantly different from CTC-negative patients (95.0% vs. 97.7%, 77.5% vs. 82.9%, 57.6% vs. 59.9%, p=0.20).ConclusionTimely change of treatment strategy for breast cancer patients with positive CTC results after surgery may improve CTC clearance rate and DFS.

目的 探讨两步法单孔腔镜乳房重建手术在早期乳腺癌治疗中的临床应用价值.方法 21例早期乳腺癌患者于乳房皮下腺体切除术即刻行两步法单孔腔镜乳房重建手术;记录手术情况、术后并发症及美容效果;术后6个月,应用BREAST Q乳腺癌患者结局量表评价患者对外观满意度和生活质量.结果 21例均顺利完成两步法单孔腔镜乳房重建手术,无中转开放手术病例;术后无假体取出、包膜挛缩发生,2例发生乳头、乳晕缺血坏死,发生率为9.5％.术后6个月,BREAST Q量表评分乳房满意度为(75.3±2.2)分,胸壁状态评分为(90.4±1.5)分,社会心理状态评分为(86.1±2.0)分,性健康程度评分为(71.4±2.6)分.结论 两步法单孔腔镜乳房重建手术在早期乳腺癌治疗中应用效果良好,患者可获较好的外观满意度和生活质量.

Objective:To analyze how the surface marker of breast cancer stem cell related to chemotherapy resistance and postoperative recurrence.Methods:The GEO public database GSE25066 and GSE32603 were used to extract clinical data including pathological evaluation of neoadjuvant chemotherapy, postoperative follow-up recurrence and recurrence time, and the expression levels of the following 18 breast cancer stem cell marker genes were extracted: CD44, CD24, EPCAM, CD49f, PROM1, CD61, ALDH1A1, CD10, ALDH1A3, ABCG2, RANK, CD47, CD166, SLUG, SOX2, SXO9, SOX10, MYC. A total of 488 patients with GSE25066 were divided into pathology compete response(pCR) group ( n=99) and residual disease (RD) group ( n=389) according to the pathological analysis. Another 418 patients with complete recurrence data was divided into recurrence group ( n=111) and non-recurrence group ( n=307), they were used for prognosis analysis. The effects of each tumor stem cell marker on chemotherapy sensitivity and tumor recurrence were analyzed by logistic, lasso and COX regression. The gene expression level of GSE32603 before and after neoadjuvant chemotherapy were used to analyze the changes after neoadjuvant chemotherapy in 166 patients, to determine the enrichment effect of chemotherapy on stem cells. Results:In chemotherapy response analysis, tumor stem cell markers CD44( OR=0.78, 95% CI: 0.63-0.98) and SANI2 ( OR=0.80, 95% CI: 0.68-0.93) were negatively correlated with neoadjuvant chemotherapy sensitivity, and CD24( OR=1.18, 95% CI: 0.97-1.47) was positively correlated with chemosensitivity. In the recurrence correlation analysis, the patients with CD44 + ( OR=1.22, 95% CI: 0.98-1.51), CD24 low( OR=0.94, 95% CI: 0.79-1.11) and SANI2( OR=1.17, 95% CI: 1.00-1.37) expression have a high recurrence rate. As chemotherapy progressed, the MME, CD49f, SOX10, MYC, and SOX9 ( P<0.05) showed an enrichment process. Conclusions:Breast cancer stem cell markers CD44 + CD24 - and SLUG can be used to predict the efficacy of neoadjuvant chemotherapy and recurrence after tumor treatment. They are relatively stable marker for studying breast cancer stem cells.

Objective:To explore the application of radiofrequency ablation in breast conserving surgery with breast carcinoma.Methods:With retrospective research methods, a total of 230 patients who were treated with radiofrequency ablation after breast conserving surgery in Beijing Friendship Hospital, Capital Medical University from January 2016 to September 2018 were selected, patients were all females, the median age was 56 yecers, ranged from 27 to 91 years, and the postoperative efficacy, patients′ satisfaction with their appearance and complications were outpatient follow-up.Results:Follow-up for 18 months showed that 2 (0.87%) patients had postoperative recurrence and metastasis, 217 (94.35%) patients were satisfied with the postoperative breast appearance, and 8 patients (3.48%) had skin burns and in- situ sclerosing. Conclusions:Radiofrequency ablation is used in breast conserving surgery, which is simple to operate and has a low incidence of complications. It can strengthen local tumor control and improve the cosmetic effect of breast after conserving surgery, worthy of clinical promotion.

Objective To evaluate the efficacy of radiofrequency ablation in breast-conserving surgery followed neoadjuvant chemotherapy.Methods Retrospective analysis of 30 cases of breast cancer patients admitted to Beijing Friendship Hospital,Capital Medical University from April 2015 to September 2018.The average age was 54.3 years and the range was 28 to 70 years.For breast invasive ductal cancer,patients underwent breast-conserving surgery after neoadjuvant chemotherapy,and then received radiofrequency ablation.The clinical and pathological characteristics,postoperative complications,recurrence and metastasis,and cosmetic effects were observed.Results All 30 patients with breast-conserving surgery successfully completed radiofrequency ablation.The average outpatient follow-up was 22.5 months,ranging from 2 to 43 months,without local recurrence or metastasis;skin burns occurred in 2 cases (6.67％),and solid nodules formed in situ in 6 cases (20.00％);cosmetic satisfaction rate was 86.67％ (26/30).Conclusions The radiofrequency ablation techniques applied in breast-conserving surgery have a high rate of technical success with relative low complication rates.And the radiofrequency ablation techniques may improve the cosmetic results after breast-conserving surgery and enhance local control.

目的 探讨绝经后女性乳腺浸润性癌的钼靶X线特征与乳腺浸润性癌组织中Ki-67表达的关系.方法 选取绝经后女性乳腺浸润性癌患者187例,收集所有患者的术前双侧乳腺钼靶X线影像资料,对钼靶X线的肿块形态、边缘、密度以及钙化的形态和分布等特征进行分析.术后行免疫组织化学染色法检测乳腺浸润性癌组织中Ki-67的表达情况,并对其与乳腺浸润性癌患者的钼靶X线特征的关系进行分析.结果 根据钼靶X线肿块形态,形态高度恶性组患者乳腺癌组织中Ki-67的阳性表达率高于形态低度恶性组(P<0.05);其中,肿块形态为圆形的患者乳腺癌组织中Ki-67的阳性表达率低于肿块形态为非圆形的患者(P<0.05).根据钼靶X线肿块边缘,边缘高度恶性组患者乳腺癌组织中Ki-67的阳性表达率明显高于边缘低度恶性组患者(P<0.01);其中,肿块边缘不清晰、毛刺征/浸润性乳腺浸润性癌患者乳腺癌组织中Ki-67的阳性表达率高于肿块边缘清晰、非毛刺征/浸润性乳腺浸润性癌患者(P<0.05).根据钼靶X线钙化形态,恶性钙化组患者乳腺癌组织中Ki-67的阳性表达率明显高于非恶性钙化组(P<0.01).结论 乳腺癌患者的钼靶X线特征能在一定程度上反映Ki-67的表达水平,可能为乳腺癌患者的预后评估并指导综合治疗提供参考.

目的 探讨超声引导下Mammotome?(麦默通)微创旋切术在乳腺肿物切除中的应用经验与价值.方法 回顾性研究2014年11月至2018年5月期间,首都医科大学附属北京友谊医院1036例患者乳腺上1133个乳腺肿物行超声引导下麦默通微创旋切术的治疗效果.对手术一般情况、术后病理类型、术后并发症发生情况、术后3个月复查结果 进行分析.结果手术治疗过程中均能清晰显示病变,引导成功率为100％,无感染,无皮肤损伤,23例出现局部血肿、133例出现局部瘀斑,局部出血发生率为13.7％.术后3个月超声随访结果显示,6例(0.5％)患者手术部位出现良性病变.结论 超声引导下麦默通乳腺微创旋切术,具有操作方便、定位准确、创伤小、并发症少、恢复快、美观等优点,在切除乳腺肿物方面可替代传统手术模式,是值得推广的微创治疗方法.

目的 增加对病理组织类型为伴有骨及软骨分化的乳腺梭形细胞化生性癌的了解,并增加相关诊治经验.方法 讨论1例伴有骨及软骨分化的乳腺梭形细胞化生性癌,自术前检查至术后16个月诊治经过,结合其余文献所涉及的相似病理组织学情况进行研究.结果 该例患者经术后病理多次取材、染色以及与外院专家会诊,最终确诊为伴有骨及软骨分化的乳腺梭形细胞化生性癌,术后随访16个月,未见局部复发及远处转移.结论 伴有骨及软骨分化的梭形细胞化生性癌是一种罕见的乳腺化生性癌,其临床表现与非特殊类型浸润性乳腺癌相似,在影像学上亦无特异表现.术前诊断困难,容易漏诊及误诊.需要术后多次取材及多块组织进行上皮标记物免疫组化染色,才能确诊,其预后的判断和临床治疗方案参照普通乳腺化生性癌制定

目的 探讨绝经后浸润性乳腺癌患者乳腺密度与病理学指标及分子分型的关系,以评价乳腺密度对浸润性乳腺癌诊治及预后指导的意义.方法 收集293例绝经后浸润性乳腺癌患者的双侧乳腺钼靶X线检查资料,根据乳腺密度不同将患者分为非致密组和致密组,分析两组患者的病理学指标(肿瘤直径、淋巴结转移情况、组织学分级、免疫组织化学指标)和分子分型.结果 293例乳腺癌患者中,非致密组患者127例,致密组患者166例.非致密组和致密组患者的肿瘤直径比较,差异有统计学意义(P﹤0.05);两组患者的淋巴结转移情况、组织学分级、雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体2(HER2)和Ki-67表达情况比较,差异均无统计学意义(P﹥0.05).非致密组患者中Luminal A型的比例高于致密组患者,HER2过表达型的比例低于致密组患者,差异均有统计学意义(P﹤0.05);两组患者中Luminal B型和Basal-like型的比例比较,差异均无统计学意义(P﹥0.05).结论 绝经后浸润性乳腺癌患者的乳腺密度与病理学指标及分子分型具有一定的关系,可能对预测患者预后具有潜在意义.

目的 探讨单孔法腔镜皮下腺体切除术治疗男性乳腺发育症的临床效果及价值.方法 回顾性分析2017年3月至2017年8月首都医科大学附属北京友谊医院普外科行单孔法腔镜皮下腺体切除术的男性乳腺发育的5例病人(其中单侧乳腺发育1例,双侧乳腺发育4例,共计9例次)的手术资料.对手术美容效果、手术时间、术后引流、并发症等进行评估.结果 9例次病变均成功完成单孔法腔镜皮下腺体切除术.术后6个月美容效果评分平均为15分,病人主观评价均为非常满意.均未发生乳头乳晕坏死、切口下方积液或血肿等并发症.结论 单孔法腔镜皮下腺体切除术治疗男性乳腺发育症较常规手术方式可获得更好的美容效果,值得进一步研究.

目的:总结保留乳头乳晕的单孔腔镜皮下乳腺切除术治疗早期乳腺癌的美容效果、手术安全性及临床疗效.方法:纳入2017年2月至2017年9月治疗的12例早期乳腺癌患者,评估单孔腔镜皮下乳腺切除术术后3个月的美容效果评分,总结手术出血量、手术时间、引流量及手术并发症等.结果:手术切口2.5 ～5.0 em,平均(3.8±0.8) cm.术后3个月美容效果评分9 ～12分,平均(11.0±1.0)分:其中非常满意5例(41.7％),比较满意7例(58.3％).术后无乳头乳晕坏死、皮瓣坏死、血肿、感染、积液、假体植入失败等并发症发生.结论:保留乳头乳晕的单孔腔镜皮下乳腺切除术并发症发生率较低,手术切口短,且小切口隐藏在上臂能遮挡的部位,术后美容效果好;手术时间、出血量、引流时间均在可接受范围.

AIM To evaluate the usefulness of localization needles under mammographic, ultrasound or fiberoptic ductoscopy guidance for non-palpable breast lesions. PATIENTS AND METHODS Eighty-three patients undergoing needle localization and biopsy of non-palpable breast lesions under mammographic, ultrasound or fiberoptic ductoscopy guidance from June 2013 to December 2014 in Beijing Friendship Hospital were included in the study. The preoperative imaging assessment, application of localization needles, surgical operation and pathological examination were recorded and analyzed retrospectively. RESULTS A total of 83 localization and biopsies were carried out, of which 27 were performed under mammographic guidance, 32 under ultrasound guidance and 24 under fiberoptic ductoscopy guidance. Twenty-seven cases of breast microcalcifications were localized under mammographic guidance and surgically removed, of which eight cases were pathologically diagnosed as malignant. Thirty-two cases of non-palpable breast lesions were localized under ultrasound guidance and 30 pathologically diagnosed, of these, four cases were pathologically diagnosed as malignant. Twenty-four cases of intraductal space-occupying lesions were localized under ductoscopy guidance and surgically removed, of which five cases were pathologically diagnosed as malignant. CONCLUSION Utilization of localization needles under mammographic, ultrasound or fiberoptic ductoscopy guidance for non-palpable breast lesions is a safe and effective procedure, and is helpful in the diagnosis of breast cancer. With the help of this procedure, more malignant lesions can be localized and surgically removed.

This study set out to investigate the clinical diagnosis and treatment strategies for malignant breast adenomyoepithelioma (AME), thus increasing the clinical knowledge on such disease. Two patients with malignant breast AME in Beijing Friendship Hospital were selected for study. Here we report the diagnosis and treatment processes in terms of the failure experience and lessons and relate our findings to those in the literature. Malignant breast AME is inclined to affect the areola area. It is recommended to conduct simple mastectomy combined with sentinel lymph node dissection due to the low sensitivity of the preoperative imaging diagnosis and difficulty in the pathological diagnosis. Malignant breast AME features strong invasiveness and vulnerability to recurrence and metastasis. Therefore, the operative schemes and clinical treatment strategies should be formulated based on the comprehensive analyses of the physical signs, imageological examinations and pathology.

乳腺淋巴瘤包括原发性乳腺淋巴瘤和继发性乳腺淋巴瘤两种类型,其发病率均比乳腺癌低.乳腺淋巴瘤通过术前临床查体及影像学检查较难与乳腺癌鉴别,误诊率较高[1].因其治疗方法 及预后与乳腺癌截然不同,术前明确诊断格外重要.而当乳腺癌与乳腺淋巴瘤共存时,术前的明确诊断则更具挑战性.本文就1例同时患有乳腺癌及系统性弥漫大B细胞淋巴瘤累及乳腺的病例进行讨论,以期为临床提供参考.

Objective To analyze the clinical efficacy of the laparoscopic breast-conserving surgery combined with radiofrequency ablation to treat the early-stage breast cancer.Methods We collected 55 patients diagnosed early-stage breast cancer in retrospect,which started from January 2014 to December 2016.Twenty-seven of them were performed the laparoscopic breast-conserving surgery combined with radiofrequency ablation while others went through laparoscopic breast-conserving surgery without radiofrequency ablation.Meanwhile,we adopted the student t-test and the chi-square test to compare results of two groups.More specific,the main indexes of this study are including the post-operative local recurrence,the incidence of fat liquefaction or the incision-infection,operation time,post-operative hospital stay and the hospitalization expense.Results The laparoscopic breast-conserving surgery combined with radiofrequency ablation group had low local-recurrence than the laparoscopic breastconserving surgery group (0 and 7.69％).Additionally,there were no statistical differences between two groups in the incidence of fat liquefaction.However,The laparoscopic breast-conserving surgery combined with radiofrequency ablation group had more hospitalization expense than the laparoscopic breast-conserving surgery group [(4.1 ± 0.7) ten thousand yuan and (2.3 ± 0.6) ten thousand yuan,P ＜ 0.05].Conclusions Although the laparoscopic breast-conserving surgery combined with radiofrequency ablation group remarkably increased the hospitalization expense because of the utility of the radiofrequency ablation related apparatus,it may provide the probability of shaving more residual tumor cell and may low down the recurrence,especially not rising up the incidence of the post-operative fat liquefaction.Therefore,this surgery method might be one of the potential developments in the minimal-invasive of early stage breast cancer.