Human umbilical cord mesenchymal stem cells (hUMSCs) belong to a multipotent stem cell population. Transplantation of icariin (ICA)-treated hUMSCs have better tissue repairing function in chronic liver injury. This study was to investigate whether the tissue-repairing effects and migration of hUMSCs after ICA treatment were regulated by circular RNAs (circRNAs). ICA was used to treat hUMSCs in vitro for 1 week and the expression profiles of circRNAs were generated using RNA sequencing. Differentially expressed circRNAs in hUMSCs after ICA intervention were screened. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis were carried out to predict the potential function of dysregulated circRNAs. There were 52 differentially expressed circRNAs (32 circRNAs up-regulated and 20 circRNAs down-regulated) with fold change ≥2.0 before and after ICA treatment. ADP-ribosylation factors were associated with the dysregulated circRNAs among Gene Ontology analysis. Kyoto Encyclopedia of Genes and Genomes analysis showed that only endocytosis pathway was associated with up-regulated circRNAs, whereas 4 pathways including homologous recombination, RNA transport, axon guidance, and proteoglycans in cancer were related to down-regulated circRNAs. Fifty-two differentially expressed circRNAs and 238 predicted microRNAs were included in circRNAs-microRNAs network. The mechanism of ICA inducing hUMSCs migration may be through regulating circRNAs expression which affects ADP-ribosylation factors protein signal pathways.

BACKGROUND: Bazi Bushen is a Chinese patented medicine with multiple health benefits and geroprotective effects, yet, no research has explored its effects on intestinal homeostasis. In this study, we aimed to investigate the effect of Bazi Bushen on intestinal inflammation and the potential mechanism of gut microbiota dysbiosis and intestinal homeostasis in senescence-accelerated mouse prone 6 (SAMP6). The hematoxylin and eosin (H&E) staining and immunohistochemistry were performed to assess the function of the intestinal mucosal barrier. The enzyme-linked immunosorbent assay (ELISA) and Western blotting were used to determine the level of intestinal inflammation. The aging-related ss-galactosidase (SA-ss-gal) staining and Western blotting were used to measure the extent of intestinal aging. The 16S ribosomal RNA (16S rRNA) was performed to analyze the change in gut microbiota composition and distribution. RESULTS: Bazi Bushen exerted remarkable protective effects in SAMP6, showing a regulated mucosal barrier and increased barrier integrity. It also suppressed intestinal inflammation through down-regulating pro-inflammatory cytokines (IL-6, IL-1 ss, and TNF-alpha) and inhibiting TLR4/NF kappa B signaling pathway (MYD88, p-p65, and TLR4). Bazi Bushen improved intestinal aging by reducing the area of SA-ss-gal-positive cells and the expression of senescence markers p16, p21, and p53. In addition, Bazi Bushen effectively rebuilt the gut microbiota ecosystem by decreasing the abundance of Bacteroides and Klebsiella, whiles increasing the ratio of Lactobacillus/Bacteroides and the abundance of Akkermansia. CONCLUSION: Our study shows that Bazi Bushen could serve as a potential therapy for maintaining intestinal homeostasis. (c) 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

肿瘤的发生发展与肿瘤微环境密切相关.肿瘤微环境中除肿瘤细胞外还包括中性粒细胞、巨噬细胞、自然杀伤细胞、T细胞等免疫细胞浸润,活化的免疫细胞对肿瘤的发生发展具有关键作用.浸润于肿瘤微环境中的中性粒细胞又称肿瘤相关中性粒细胞(TANs),是肿瘤微环境的重要组成部分.TANs可通过调控其他免疫细胞的功能、中性粒细胞外诱捕网的形成以及极化状态等参与肿瘤的形成、发展和转移,并可通过多种信号通路影响肿瘤的进展,对肿瘤具有双向调控作用.因此,未来深入研究TANs在肿瘤调控中的具体机制,可为肿瘤患者的免疫治疗提供新策略.

中西医结合诊疗模式是在长期的理论、基础研究与临床实践中不断总结而逐渐形成的"辨证论治、病证结合"诊疗模式.通过西医以疾病为主的模式化诊疗和中医以个体为主的个性化诊疗相结合,发挥出各自的优势使治疗效果最大化,更好地服务于患者,但中西医结合并不是单纯地将中医与西医简单相加.如何将两者结合发挥出最大优势从而造福社会,走出中西医结合的瓶颈是现阶段应思考的问题.高永翔教授认为中西医结合的诊疗过程应该遵循:1)中医、西医取长补短.2)发挥中西医协同效应.3)中西药合用的减毒增效.4)中西药合用的君臣佐使.5)考虑患者的依从性.6)关注国家、患者的经济负担.最后,高永翔教授表示,应全力支持中西医结合医学学科的发展,大力扶持中西医结合科室的建立.

目的:通过网络药理学的方法探讨痛泻要方对肠易激综合征和抑郁症异病同治的作用机制.方法:本研究首先利用中药系统药理学分析数据库(TCMSP)、BATMAN-TCM数据库结合文献挖掘的方法检索痛泻要方的4味中药所有化学成分;通过TCMSP数据库、UniProt数据库和Cytoscape 3.7.2软件构建"中药-化合物-靶点"网络图;使用STRING 11.0数据库和Cytoscape 3.7.2软件进行共有靶点的蛋白质间相互作用分析筛选关键共有靶点,并使用DAVID 6.8数据库和Omicshare在线分析工具对关键共有靶点进行GO分析和KEGG分析.结果:根据化学成分的口服生物利用度(OB)≥30％和类药性指数(DL)≥0.18筛选到痛泻要方共40个活性化合物,139个药物作用靶点,构建中药-成分-靶点网络;检索到肠易激综合征疾病靶点共3554个,抑郁症靶点12610个,与痛泻要方139个作用靶点取交集,获得共有靶点104个,使用STRING 11.0数据库和Cytoscape 3.7.2软件进行共有靶点的蛋白质间相互作用分析,筛选得到关键共有靶点50个;DAVID进行GO功能富集分析得到生物过程350个,细胞组成39个,分子功能77个,KEGG富集分析获得92条信号通路,靠前排序的信号通路有TNF、Toll-like receptor、NOD-like receptor、Prolactin、FoxO和PI3K-Akt等信号通路,主要与氧化应激、免疫调控、炎症、凋亡以及内分泌代谢相关.结论:痛泻要方异病同治肠易激综合征和抑郁症的主要作用机制与氧化应激、免疫调控、炎症、凋亡以及内分泌代谢相关,为进一步试验验证潜在药理学机制及临床拓展应用提供参考依据.

The present study aimed to identify key genes as potential biomarkers for early nephrotoxicity induced by aristolochic acid (AA) in embryonic stem cells (ESCs). An MTT assay was performed to determine the cytotoxicity of AA in ESCs. Differentially expressed genes (DEGs) were identified using the DNA-Chip Analyzer following microarray analysis. Gene Ontology analysis was performed to determine functional terms enriched by the DEGs in the categories biological process, cellular component and molecular function. Furthermore, the DEGs were subjected to Kyoto Encyclopedia of Genes and Genomes analysis to determine pathways they were accumulated in. Furthermore, a protein-protein interaction network was constructed using Cytoscape 3.2 software. Tumor protein 53 apoptosis effector (Perp), cation transport regulator-like 1 (Chac1), adrenoceptor β2 and Wnt6 were selected for confirmation by reverse transcription-quantitative (RT-q) PCR analysis. A total of 72 DEGs (49 upregulated and 23 downregulated) were identified. The DEGs were enriched in functional terms and pathways associated with nephrotoxicity and participated in 92 pathways. A total of two hub genes, fructose-1,6-bisphosphatase (Fbp)1 and Fbp2, were filtered out from the interaction network. Perp and phorbol-12-myristate-13-acetate-induced protein 1 were demonstrated to have vital roles in the p53 signaling pathway which was indicated in the interaction network. The results of the RT-qPCR analysis were consistent with the microarray data. Taken together, the present study suggested that hub genes involved in the p53 pathway, including Fbp1, Fbp2 and Perp, may serve as potential biomarkers for early nephrotoxicity induced by AA.

Kang-Xian (KX) pills have been clinically used for the treatment of chronic hepatic injury (CHI). However, the mechanisms of KX on CHI remain unknown. The aim of this study mainly focused on the anti-inflammatory effects of KX in a CHI mouse model based on modulating gut microbiota and gut permeability. We first established a CHI model using carbon tetrachloride (CCl4) and treated it with KX. The anti-inflammatory effects of KX on CHI model mice and the changes in gut permeability after KX treatment were also investigated. 16S rRNA analysis was used to study the changes of gut microbiota composition after KX treatment. In addition, gut microbiota was depleted using a combination of antibiotics in order to further confirm that KX could inhibit the inflammatory response and decrease gut permeability to treat CHI by modulating the gut microbiota. Results showed that KX treatment significantly improved liver function in CHI model mice. KX could also increase the levels of tight junction proteins in the colon and decrease the expression of proinflammatory cytokines in the liver. 16S rRNA analysis indicated that KX treatment affected the alpha and beta diversities in CHI model mice. Further analysis of 16S rRNA sequencing indicated that KX treatment increased the ratio of Firmicutes to Bacteroidetes at the phylum level. At the genus level, KX treatment increased the relative abundance of Lactobacillus, Bacteroides, and Akkermansia and decreased the relative abundance of Ralstonia, Alloprevotella, and Lachnoclostridium. However, KX could not alleviate CHI after depleting the gut microbiota. The effects of KX on gut permeability and inflammatory response in the liver were also decreased following the depletion of gut microbiota. In conclusion, our current study demonstrated that gut microbiota was significantly affected during CHI progression. KX could inhibit the inflammatory response and decrease the gut permeability in CHI model mice through modulating the gut microbiota.

目的:了解本院抗反转录病毒的用药情况,促进免费药物的临床合理用药,并保证患者用药安全.方法:选取2016—2018年每月固定2 d的免费用药处方,利用Excel表格统计出每种药的使用情况和药物之间的联合使用情况.结果:本院3年中抗反转录病毒免费用药使用情况主要以核苷类反转录酶抑制剂( NRTIs)、非核苷类反转录酶抑制剂(NNRTIs)、蛋白酶抑制剂(PIs)3类药物为主,药物以富马酸替诺福韦二吡呋酯片、拉米夫定片和依非韦伦片三药联合应用为主,3年分别占总处方数的48. 79% 、49. 52%和50. 18% ,用量呈逐年递增状态.结论:本院的抗反转录病毒免费用药的使用情况与2018年的艾滋病诊疗指南基本一致,使用情况基本合理.

Objective: Fatigue has become one of the major threats to human health in the 21st century. Traditional Chinese medicine (TCM), which proved to be safer and more effective, has become a hot spot in antifatigue research. Human placenta, also called "Ziheche", has drawn great attention in the antifatigue effect since the Tang dynasty. However, the shortage of human placenta restricts wide usage of it. According to the theory of TCM, sheep placenta (SP) also has the effect of nourishing blood, tranquilization, nourishing skin, and prolongation of life. The aim of this study was to examine the antifatigue effects of sheep placenta peptide (SPP), an extract of sheep placenta, in mice and the mechanism was also studied. Methods: Peptide from fresh SP was extracted via enzymolysis. SPP (0.13 g/kg) and soybean peptide (0.65 g/kg) were administrated orally and daily to mice for four weeks. Antifatigue effects of SPP were estimated on weight-loaded swimming test; A non-weight-loaded swimming test was conducted to elucidate underlying the mechanisms of the anti-fatigue effects. Results: Administration of SPP prolonged the weight-loaded swimming time in mice. In addition, SPP significantly decreased the levels of muscle malondialdehyde (MDA) and serum lactic acid (LD), and increased the activities of muscle glutathione peroxidase (GSH), and superoxide dismutase (SOD) and liver glycogen in mice after non-weight-loaded swimming test. Moreover, the electron microscope observation showed that the muscle fiber bundle ranked neatly, the H band, I band, Z line as well as M line were clear and the numbers of mitochondria was normal though some of the mitochondria were swell in SPP treated mice after non-weight-loaded swimming test. Conclusion: SPP possesses potent abilities for antifatigue; Increasing the anti-oxidant activities and energy reserve as well as improving the ultrastructures in gastrocnemius muscle cells, which may be the mechanisms of SPP exerting its antifatigue effects. (C) 2018 Tianjin Press of Chinese Herbal Medicines. Published by Elsevier B.V. All rights reserved.

目的:了解本院布鲁氏菌病中保肝药的用药情况,促进保肝药的临床合理用药,并保证患者用药安全.方法:选取2017年1—12月本院门诊布鲁氏菌病的病例中,全部联合使用保肝药物的电子处方,运用频数分析的方法研究其处方的组成,各类保肝药的使用频次、频率及其临床合理使用注意事项.结果:布鲁氏菌病的治疗中联合用保肝药以水飞蓟素和五酯片为主,多数采用中西医结合治疗方法.结论:应严格按照药物适应症用药,降低不合理用药发生率,保证患者用药安全.

基于数据挖掘及辨证论治探讨国家药品标准中中药治疗肝病的用药特点.分析《中国药典》2015年版、《中国药典·临床用药须知》2010年版(中药卷)和中华人民共和国卫生部药品标准中治疗肝病的经典方剂,建立数据库;采用Excel统计分析软件进行频数分析,采用SAS 9.1统计分析软件作为数据挖掘平台,进行关联规则分析.共获得肝病治疗用中药复方114个,涉及药物246种;以使用频次≥10次的27种作为主要药物进行分析,归为补虚药、理气药、活血化瘀药、清热药、利水渗湿药、化湿药、解表药、泻下药、收涩药9大类;经关联规则分析,共获得两联药对18对,三联药对20对;结合病症分析了用药特点.结合数据挖掘和辨证论治有助于解读中药复方治疗肝病的用药规律和特点.

肝损伤是各类肝性疾病肝细胞损伤共同的病例特征.随着传统医学的发展,一些单方和复方中草药在肝损伤保护方面表现出了较大的优势.多糖是一类天然大分子物质,具有抗肿瘤、抗氧化、抗突变、抗病毒、降血脂等生物活性.近来研究表明,多种中草药的多糖类成分都具有对肝损伤的保护作用.本文对多糖类化合物对肝损伤动物模型的作用特点、作用机制和原理进行归纳和总结.

五味子及南五味子具有收敛固涩、益气生津、补肾宁心的作用,用于久咳虚喘、梦遗滑精、遗尿尿频、久泻不止、自汗盗汗、津伤口渴、内热消渴、心悸失眠.五味子多糖是五味子及南五味子的主要化学成分之一,本文对五味子多糖保肝、抗缺氧、抗疲劳、增强免疫力、抗肿瘤、镇静催眠等方面药理作用进行分析综述.

Objective To analyze use of oral Chinese patent drugs for liver disease treatment in The Second People’s Hospital of Tianjin during 2010—2013, and to discuss the feasibility and practicability of DU 90%in the evaluation of clinical drug utilization, so as to provide scientific evidence for the rational usage. Methods The utilization data including consumption sum, DDDs, and DDDc of oral Chinese patent drugs for liver disease treatments in The Second People’s Hospital of Tianjin during 2010—2013 were analyzed statistically, and the use of DU 90% was analyzed. Results The consumption sum of oral Chinese patent drugs for liver disease treatment increased by 21.70%, 9.15%, and 26.43%from 2010 to 2013, and there was an upward trend. Drug varieties high ranking in the consumption sum were relatively stable, including antipyretic and antidote drugs, blood-activating, stasis-eliminating drugs and Qi-restoring drugs. DDDs of DU 90%drugs were relatively stable, and the sales sum and DDDs of synchronicity was better. DDDc of most drugs were low, and the composition ratio increased in 2013. Conclusion The use of oral Chinese patent drugs for liver disease treatment in The Second People’s Hospital of Tianjin during 2010—2013 is basically reasonable, and DU 90%can be used as an effective tool, which is applied to the prescription of evaluation process.

随着社会节奏的加快,长期紧张的工作和学习都容易导致疲劳的发生.疲劳(fatigue)是一个复杂的生理生化过程,发生机制比较复杂.我国具有丰富的中药资源,近年来不少学者在中药抗疲劳的作用机制方面做了大量工作.研究表明,许多单味中药及其成分如当归多糖、人参活性成分人参皂苷、黄酮类化合物葛根素以及补虚类中药西洋参、紫河车和清热药桑叶等;中药复方制剂如益气生津方、理气调补汤等都具有很好的抗疲劳作用.本文就中药抗疲劳的作用机制,从中药有效成分、单味中药和复方制剂三方面做一综述,为指导临床用药提供药理学依据.

Object: To establish an HPLC method for determination of baicalin in Kangxian pills.Methods: The separation was performed on a Phenomenex C_(18) column with methanol-water-phosphoric acid(47∶53∶0.2) as mobile phase.The flow rate was 1.0 ml/min and the detection wavelength was set at 280 nm.Results: The linearity was obtained within a range of 0.1~1.0 μg(r=0.999 5).The average recovery was 99.82% and RSD of 1.76%(n=5).Conclusion: The method is easy and reproducible and can be used for quality control of Kangxian pills.