Experimental teaching is an important part of medical undergraduate and postgraduate education. In the process of teaching and scientific research experiments, various dangerous chemicals are inevitably used. Based on the requirements of hazardous chemicals management and the current situation of hazardous chemicals in school of basic medical sciences, this paper puts forward corresponding management suggestions and countermeasures. Through the formulation of a three-level management system for university, colleges and laboratories, promoting the use of intelligent hazardous chemical cabinets, strengthening the organization of hazardous chemical emergency drills and improving the safety awareness of teachers and students, etc, the safety risks of hazardous chemicals are reduced and safety accidents do not occur during the storage and use of various hazardous chemicals.

肝内胆管细胞癌(ICC)是发病率仅次于肝细胞癌的肝脏恶性肿瘤,它的恶性程度高、术后易复发,且早期无典型症状,大多数患者在确诊时已处于晚期.诊断主要依赖于增强CT、MRI和实验室检查.肝切除术是ICC首选的治疗方法,完整的切缘阴性切除和保留足够残留肝是影响手术预后的重要因素.淋巴结清扫、卡培他滨辅助化疗已被证实对患者有益.局部治疗、分子靶向治疗、免疫治疗等新疗法发展迅速,为晚期ICC患者带来了希望.传统疗法与新疗法的结合为ICC提供新的诊疗思路.

为解决地方医科院校本科教育中普遍存在的重课内教学、轻课外创新实践,学生的创新意识、创新实践能力亟待提高等问题,文章探索在课内理论与实验教学基础上,构建可训练并提高学生的创新思维与创新实践能力、科研基本技能的基础医学创新实践平台,试行双导师制,并摸索大学生创新实践平台运行的有效机制与管理模式.6年多的实践表明,该创新实践平台建设成效明显,大学生创新性思维与创新实践能力得以有效提高.

目的:基于网络药理学和实验验证探究防己茯苓汤治疗缺血再灌注急性肾损伤(AKI)的作用机制.方法:通过中药系统药理学数据库与分析平台(TCMSP)数据库和文献挖掘收集防己茯苓汤活性成分,利用SwissTargetPrediction预测活性成分作用靶点;借助GeneCards、在线人类孟德尔遗传数据库(OMIM)、疾病相关的基因与突变位点数据库(DisGeNET)、治疗靶标数据库(TTD)收集AKI靶点;利用STRING平台构建蛋白质-蛋白质相互作用(PPI)网络;采用Metascape平台对核心靶点进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)通路分析;利用Cytoscape软件构建药物-活性成分-作用靶点-疾病网络图和活性成分-作用靶点-信号通路网络图;使用AutoDock进行分子对接;最后采用动物实验验证分析结果.结果:筛选出防己茯苓汤活性成分137种及药物靶点858个,AKI疾病靶点1 294个,防己茯苓汤治疗AKI作用靶点267个,其中磷脂酰肌醇3-激酶调节亚基1(PIK3R1)、磷脂酰肌醇3-激酶催化亚单位α(PIK3CA)、原癌基因酪氨酸蛋白激酶(SRC)、蛋白激酶B1(Akt1)、促分裂原活化的蛋白激酶3(MAPK3)为防己茯苓汤治疗AKI的关键靶点,GO富集分析得到1 609个结果,主要涉及细胞对脂质的反应、膜筏、蛋白激酶活性等,KEGG通路分析则与PI3K/Akt信号通路、趋化因子信号通路、Toll样受体(TLR)信号通路等140条通路有关,分子对接则发现核心活性成分和关键靶点均有良好的结合能力,苏木精-伊红(HE)染色结果表明防己茯苓汤能明显改善AKI的病理学状态,血清学结果则显示血清肌酐(SCr)、尿素氮(BUN)水平明显下降.结论:该研究初步探讨了防己茯苓汤治疗AKI的作用机制,发现防己茯苓汤治疗AKI具有多成分、多靶点、多通路的作用特点,为后续进一步深入研究具体作用机制奠定基础.

以2019级临床医学专业的单纯线上教学作为对照,在2020级全面开展以学生为中心的线上+线下混合式教学法,探讨其在组织胚胎学实验教学中的应用效果.实施过程以教师为主导,开展小组讨论、翻转课堂、随堂测试等多种学习形式,将知识传授、能力培养和价值塑造融为一体.实践表明,线上线下混合式教学法能全面调动学生学习的积极性,明显提高学生自主学习能力、综合素质和课程实验教学效果.

目的:探讨高校研究生心理健康教育现状及对策.方法:随机选取2018年5月至2019年5月广州医科大学的研究生1000例,采用一般情况调查表、症状自评量表(SCL-90)对高校研究生心理健康教育现状进行分析.结果:1000例高校研究生中,躯体化阳性53例,焦虑阳性69例,抑郁阳性82例,偏执阳性70例,敌对阳性82例,恐怖阳性54例,精神病性阳性63例,强迫症状阳性182例,人际敏感阳性92例,其他阳性75例,阳性率分别为5.3％、6.9％、8.2％、7.0％、8.2％、5.4％、6.3％、18.2％、9.2％、7.5％.高校研究生的SCL-90总分、躯体化、焦虑、抑郁、偏执、敌对、强迫症状、人际敏感评分均显著低于全国成人常模(P<0.05),但二者的恐怖、精神病性评分之间的差异均不显著(P>0.05).20-25岁、>30岁高校研究生的躯体化、抑郁、恐怖评分均显著低于26-30岁(P<0.05),但20-25岁、>30岁高校研究生的躯体化、抑郁、恐怖评分之间的差异均不显著(P>0.05).回归分析显示,高校研究生心理健康影响因素包括年龄、是否应届、专业满意等级、压力等级(P<0.05).结论:高校研究生心理健康教育现状不容客观,需要积极采取有针对性对策改善这一现状.

目的 观察VEGF对TMP治疗大鼠脊髓损伤的影响.方法 实验大鼠随机分为假手术组、 生理盐水组、TMP组和TMP+Avastin组,观察术后8周脊髓损伤处VEGF、vWF和IL-8表达,以及神经纤维再生、 后肢运动功能.结果 TMP+Avastin组内源性VEGF表达减少,限制血管新生及炎性反应(P<0.05).对于神经纤维再生及其后肢运动,TMP组优于生理盐水组(P<0.05),TMP+Avastin组的效果更优(P<0.05).结论 内源性VEGF阻碍了TMP对大鼠脊髓损伤的修复,Avastin通过降低炎性反应提高TMP治疗大鼠脊髓损伤的疗效,促进神经纤维再生及后肢运动功能恢复.

目的 探讨术前肠镜黏膜下注射纳米碳定位在早期大肠癌腹腔镜手术中的临床价值.方法 回顾性分析2014年1月至2017年6月广州市第一人民医院收治的46例早期大肠癌患者的临床资料,其中,采用术前肠镜黏膜下注射纳米碳定位22例(术前纳米碳定位组);实施腹腔镜手术联合术中肠镜定位24例(术中肠镜定位组).对比分析两组腹腔镜手术的手术时间、术中出血量、术后排气时间、住院时间.结果 两组患者均成功准确找到病变部位,并顺利完成腹腔镜手术.术前纳米碳定位组的手术时间显著短于术中肠镜定位组(P<0.05).两组术中出血量、术后排气时间、住院时间比较,差异均无统计学意义(P>0.05).结论 术前肠镜黏膜下注射纳米碳可节约早期大肠癌腹腔镜手术的手术时间,病变定位方便、准确、有效,同时可节省大量人力物力.

Objective To determine the association of high IL-1β levels with the migration of lung cancer H460 cells with acquired resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).&nbsp;Methods The resistant cells were referred to as H460-TR in this study. IL-1β levels in H460-TR cells and the parent H460 (H460-WT) cells were measured through RT-PCR, Western blot, and enzyme-linked immunosorbent assay. The migration capacity of the cells was determined using the migration transwell assay. The extent of migration and the activation of phosphatidyl-inositol 3-kinase/serine-threonine kinase (PI3K/Akt) were detected in H460 cells treated with or without human recombinant IL-1 or IL-1R antagonist. Results Migration capacity of H460-TR cells in the conditioned medium and its IL-1β level were higher than those of H460-WT cells . The migration capacity and Akt activation were consistent with the IL-1β level in lung cancer H460 cells. Conclusions Significantly elevated IL-1β expression in cancer cells is associated with the high migration capacity of H460-TR cells, and Akt activation. Akt signaling as the downstream pathway of IL-1β and IL-1βmay function as a therapeutic target for metastatic lung cancer.

Objective To establish acute kidney disease mouse model caused by aristolochic acid Ⅰ.Methods The mice were divided into experimental groups and control group,experimental groups were respectively given one-offinjection of aristolochic acid Ⅰ 20,50,100 mg/kg body weight in mice,which leaded to acute renal injury in mice.Took blood and kidney tissue samples on the 3rd,7th day of the experiment,tested related renal function indexes and tissue morphology.Results After the injection of aristolochic acid Ⅰ,the contents of creatinine,blood urea nitrogen of mice increased,the kidney slice staining showed kidney tissue morphological changes were associated with dose and time.Conclusion Application of large dose of aristolochic acid Ⅰ successfully established a mouse model of acute kidney disease.

Transforming growth factor (TGF-β1) is among the strongest factors of liver fibrogenesis, but its association with Schistosoma-caused liver fibrosis is controversial. Tissue transglutaminase (tTG) is the principal enzyme controlling TGF-β1 maturation and contributes to Sj-infected liver fibrosis. Here we aim to explore the consistency between tTG and TGF-β1 and TGF-β1 source and its correlation with liver fibrosis after Sj-infection. TGF-β1 was upregulated at weeks 6 and 8 upon liver fibrosis induction. During tTG inhibition, TGF-β1 level decreased in sera and liver of infected mice. TGF-β1 showed positive staining in liver containing Sj adult worms and eggs. TGF-β1 was also detected in Sj adult worm sections, soluble egg antigen and Sj adult worm antigen, and adult worms' culture medium. The TGF-β1 mature peptide cDNA sequence and its extended sequence were amplified through RT-PCR and RACE-PCR using adult worms as template, and sequence is analyzed and loaded to NCBI GenBank (number GQ338152.1). TGF-β1 transcript in Sj eggs was higher than in adult worms. In Sj-infected liver, transcriptional level of TGF-β1 from Sj, but not mouse liver, correlated with liver fibrosis extent. This study provides evidence that tTG regulates TGF-β1 and illustrates the importance of targeting tTG in treating Sj infection-induced fibrosis.