Background: Previous research suggests that maternal parenting stress is a significant predictor of socialemotional problems in children. However, little is known regarding the mother-child relationship and the effect of maternal adverse childhood experiences (ACEs) on this association. Methods: Three waves of longitudinal panel data were collected from 2893 Chinese preschoolers with a follow-up interval of 6 months. The mothers of preschoolers were asked to complete anonymous questionnaires concerning demographic variables, maternal ACEs and parenting stress in Wave 1, mother-child relationships in Wave 2, and children's social-emotional problems in Wave 3. The parallel mediation model was conducted to analyze the mediating role of three dimensions of mother-child relationships, and the moderation model was conducted to examine the moderating role of maternal ACEs. Results: The results showed that maternal parenting stress predicted children's social-emotional problems directly or indirectly through the mother-child relationship, with an intimate mother-child relationship mediating this main effect negatively but a conflicted and dependent mother-child relationship mediating this main effect positively. In addition, moderating results indicated that the main effect of maternal parenting stress on children's social-emotional problems was more marked among participants with at least one maternal ACEs than those without maternal ACEs. Furthermore, the moderating effect was only detected in children whose mothers had a high school education or less. Limitations: The subjectivity of mothers' reports may somewhat reduce the credibility due to the possible overestimation or underestimation of children's social-emotional problems. Conclusion: These findings provide new evidence for the effects of maternal parenting stress on children's socialemotional development and highlight the need for more attention to children with mothers having ACE exposure, lower educational level and poor parent-child relationships.

Background: Previous researches found that maternal ACEs not only affect the psychological behavior of preschool children, but also have direct or indirect negative effects on the executive functions (EFs) and cognition of offspring. And, the possible social psychological mechanism between maternal ACEs and preschool children's EFs is still not clear. Objectives：This study mainly tries to understand the association between parenting stress and child maltreatment in maternal ACEs and children's EFs through longitudinal cohorts. Participants and Setting: Participants were 2160 preschool children and their mothers who finally completed baseline and 3 waves of follow-up. Methods: Using a cohort study, a baseline survey of junior kindergartens was carried out in June 2021 and followed up every six months, with a total of 3 follow-ups. Results: We found that EFs in preschoolers were significantly positively correlated with maternal ACEs, parenting stress, physical assault, psychological aggression, neglect and nonviolent discipline (r = 0.180, 0.386, 0.274, 0.302, 0.189, 0.148, respectively, P<0.01). Further, parenting stress and child maltreatment showed a chain mediating effect between maternal ACEs and EFs in preschoolers, and the total indirect effects accounted for 70.56%, 78.69%, 65.38%, and 68.07% of the total effect, respectively. Conclusions: This study found that maternal ACEs have a significant impact on the EFs of preschool children, and parenting stress and child maltreatment are the mediating factors of their association, revealing the potential mechanism between the two associations from the perspective of social psychology.

Emerging evidence indicates the critical roles of circular RNAs in the development of multiple cancers, containing hepatocellular carcinoma (HCC). Herein, our present research reported the biological function and mechanism of circ_0027791 in HCC progression. Circ_0027791, microRNA-496 (miR-496), programmed cell death ligand 1 (PDL1), and methyltransferase-like 3 (METTL3) levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Cell viability, proliferation, invasion, and sphere formation ability were detected using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, 5-ethynyl-2'-deoxyuridine, transwell, and sphere formation assays. Macrophage polarization was detected using flow cytometry assay. To understand the role of circ_0027791 during the immune escape, HCC cells were cocultured with peripheral blood mononuclear cells or cytokine-induced killer (CIK) cells in vitro. A xenograft mouse model was applied to assess the function of circ_0027791 in vivo. After prediction using circinteractome and miRDB, the binding between miR-496 and circ_0027791 or PDL1 was validated based on a dual-luciferase reporter assay. Interaction between METTL3 and circ_0027791 was determined using methylated RNA immunoprecipitation (MeRIP)-qPCR, RIP-qPCR, and RNA pull-down assays. Circ_0027791, PDL1, and METTL3 expression were upregulated, and miR-496 was decreased in HCC patients and cells. Moreover, circ_0027791 knockdown might repress proliferation, invasion, sphere formation, M2 macrophage polarization, and antitumor immune response. Circ_0027791 knockdown repressed HCC tumor growth in vivo. In mechanism, circ_0027791 functioned as a sponge for miR-496 to increase PDL1 expression. In addition, METTL3 mediated the m6A methylation of circ_0027791 and stabilized its expression. METTL3-induced circ_0027791 facilitated HCC cell progression partly regulating the miR-496/PDL1 axis, which provided a new prognostic and therapeutic marker for HCC.

Abstract Background Previous researches found that maternal adverse childhood experiences not only affect the psychological behavior of preschool children, but also have direct or indirect negative effects on the executive functions and cognition of offspring. And, the possible social psychological mechanism between maternal adverse childhood experiences and preschool children’s executive functions is still not clear. Objectives This study mainly tries to understand the association between parenting stress and child maltreatment in maternal adverse childhood experiences and children’s executive functions through longitudinal cohort. Participants and setting Participants were 2160 preschool children and their mothers who finally completed baseline and 3 waves of follow-up. Methods Using a cohort study, a baseline survey of junior kindergartens was carried out in June 2021 and followed up every six months, with a total of 3 follow-ups. Results We found that executive functions in preschoolers were significantly positively correlated with maternal adverse childhood experiences, parenting stress, physical assault, psychological aggression, neglect and nonviolent discipline (r = 0.180, 0.386, 0.274, 0.302, 0.189, 0.148, respectively, P < 0.01). Further, parenting stress and child maltreatment showed a chain mediating effect between maternal adverse childhood experiences and executive functions in preschoolers, and the total indirect effects accounted for 70.56%, 78.69%, 65.38%, and 68.07% of the total effect, respectively. Conclusions This study found that maternal adverse childhood experiences have a significant impact on the executive functions of preschool children, and parenting stress and child maltreatment are the mediating factors of their association, revealing the potential mechanism between the two associations from the perspective of social psychology.

目的:研究安徽石台县日本血吸虫的核糖体DNA第二内转录间隔区(rDNA-ITS2)基因和线粒体细胞色素C氧化亚基1基因(mtDNA-COX1)的遗传变异.方法:收集两种不同来源的阳性钉螺(石台和南京),逸出尾蚴后感染小鼠,每只小鼠感染(20±2)尾蚴,35天后解剖小鼠,收集血吸虫成虫,PCR-RFLP扩增成虫的rDNA-ITS2和mtDNA-COX1基因,限制性核酸内切酶Taq Ⅰ水解,观察结果;同时收集小鼠的肝脏和血液,HE染色观察肝脏肉芽肿的大小,ELISA检测小鼠血清中的谷丙转氨酶(ALT).结果:石台组和南京组均获得500 bp的ITS2序列和1400 bp的mtDNA-COX1序列;两组的ITS2序列经Taq Ⅰ酶切后,得到一样的片段;而mtDNA-COX1序列经Taq I酶切后,两组结果不同,石台组在1000 bp检测到1条清晰的条带,而南京组在此处无任何条带;两组小鼠的肝脏纤维化病理结果与谷氨酸转氨酶测定结果均无明显差异.结论:石台组和南京组的日本血吸虫虽然在致病性方面具有相似性,但在mtDNA-COX1基因序列存在差异,本研究为今后日本血吸虫的种群遗传结构研究提供科学依据.

《病原生物学与免疫学》作为医学生的重要基础课程,新冠疫情下,为了加强线上课堂教学管理,严格教学纪律,保证整体和系统的课程教学,避免课程随意化、碎片化,某校教研室探索"雨课堂+腾讯极速课堂"混合式教学模式,即依托腾讯极速课堂,开展线上教学,同时结合雨课堂课前预习、课后复习和测试,依托班级QQ群与学生沟通联系.实践证实:这种新型线上混合式教学模式能培养学生的自学能力,提高学生学习的积极性和主动性及学习效果.

Background: Depressive symptoms are the most common mental disorder among adolescents, and its prevalence has been increasing in recent years. Although childhood maltreatment is a contributing factor to depressive symptoms among adolescents, the underlying mechanism of how this factor causes depressive symptoms is unclear.Objective: This study aimed to investigate the mediating effects of parent-child relationship on the association between childhood maltreatment and depressive symptoms among adolescents and to identify how sex and only child status affect this association.Methods: A total of 14,500 middle school students were randomly selected from four cities (Shenzhen, Zhengzhou, Nanchang, and Guiyang) in China. A survey questionnaire was admin-istered to collect information on childhood maltreatment, parent-child relationship, and depressive symptoms. Pearson's correlation analysis was used in analyzing the relationship, Bootstrap method was used to test the mediating effects. A moderated mediation analysis has been used to determine the moderated mediation effect.Results: Childhood maltreatment significantly negatively correlated with parent-child relationship and positively correlated with depressive symptoms (P < 0.001). The indirect effect of parent -child relationship accounted for 20.60% of the total effect between childhood maltreatment and depressive symptoms in all respondents and differed by sex (boys, 17.00%; girls, 25.23%) and only child status (only child, 15.61%; child with siblings, 23.49%). Besides, the indirect effect of the mediation model was moderated by sex and only child status.Conclusions: Childhood maltreatment increased the risk of depressive symptoms, and this rela-tionship was partially mediated by parent-child relationship, sex and only child status have moderated the indirect relationship. These findings showed efforts aimed at enhancing the parent-child relationship may prevent or reduce the prevalence of depressive symptoms among adolescents, especially in girls and children with siblings.

目的 探讨Kiss-1基因表达与过表达对直肠癌细胞SW620增殖的影响.方法 培养人直肠癌SW620细胞,随机将其分为3组:空白对照组(未转染)、实验组(转染pIRES2-Kiss-1)、阴性对照组(转染pIRES2空载体).采用免疫荧光染色和免疫细胞化学染色观察直肠癌SW620细胞中Kiss-1的表达情况;Western blot检测转染pIRES2-Kiss-1基因后直肠癌SW620细胞中Kiss-1的表达;采用CCK-8法、MTT法检测转染对SW620细胞增殖的影响.结果 免疫重组质粒pIRES2-Kiss-1成功转染至直肠癌SW620细胞,与空白对照组和阴性对照组相比较,实验组中Kiss-1蛋白高表达,差异有显著性(P<0.05);3组细胞培养48、72h后,与空白对照组和阴性对照组SW620细胞数相比较,实验组明显降低(P<0.05).结论 Kiss-1基因在直肠癌SW620细胞中能高效、稳定地表达;Kiss-1基因可有效降低直肠癌SW620细胞的增殖.

目的 探讨将信息化手段融入《人体解剖生理学》实验教学,实现实验教学"翻转课堂"的教学模式,对实验教学的影响.方法 以心音听诊和动脉血压测量作为教学内容.以18级药学1班作为实验组采用翻转课堂教学模式,17级药学1班作为对照组采用传统教学方法.两组采用同一实验考核模式,对两组实验考核结果 进行比较、分析.结果 实验组考核成绩心音听诊和动脉血压测量成绩分别为(9.67±0.65)分和(8.38±1.17)分,明显优于对照组的(9.23±0.76)分和(7.85±0.87)分(P<0.05).结论 以信息技术为载体的翻转课堂教学模式,对学生的自主学习、实操动手、语言表达等综合能力提升有较大帮助,教学效果优于传统实验教学方法,值得进一步研究和推广.

The lack of reliable plasma biomarkers limits their use in the diagnosis of gastric cancer (GC). The current study aimed to determine whether plasma nesfatin-1 can be used as a novel non-invasive biomarker for the diagnosis of GC. The levels of nesfatin-1 in 40 patients with GC and 40 healthy individuals, who were selected from the Chaohu Hospital Affiliated to Anhui Medical University, were assessed. ELISA was used for the measurement of plasma nesfatin-1 levels, while immunohistochemistry was applied to determine Ki67 protein expression in GC and normal gastric tissues. The diagnostic value of plasma nesfatin-1 for GC was further assessed using receiver operating characteristic (ROC) curve analysis. The results revealed that, compared with the controls, the mean nesfatin‐1 levels in patients with GC were significantly increased. Furthermore, the protein expression of Ki67 in GC tissue was significantly upregulated compared with that in normal gastric tissue. Plasma nesfatin-1 levels were also demonstrated to be correlated with Ki67 protein expression in GC tissues. Additionally, ROC curve analysis indicated the potential diagnostic value of nesfatin-1, and the area under the ROC curve (AUC) for nesfatin‐1 was 0.857 (95% confidence interval, 0.769-0.946). At a threshold nesfatin-1 level of 1.075 ng/ml, the optimal sensitivity and specificity were 70.0 and 95.0%, respectively, in discriminating patients with GC from healthy controls. These results indicated that plasma nesfatin-1 may serve as a novel biomarker for the diagnosis of GC and determination of GC cell proliferation.

岗位设置管理为医疗卫生事业单位人事制度改革的重要内容,是医疗卫生事业单位自身发展的需要,也是目前众多公立医院管理部门亟待面对的重要问题.本文通过文献分析、问卷调查以及访谈方法,对于以岗位设置管理为基点的人才管理的现状、意义、面临的主要问题、实施的要求和实施过程中应重点关注的问题进行了总结.旨在为公立医院管理部门和岗位设置改革的实施以及相关工作的开展提供思路和依据.

The lack of reliable plasma biomarkers limits their use in the diagnosis of gastric cancer (GC). The current study aimed to determine whether plasma nesfatin-1 can be used as a novel non-invasive biomarker for the diagnosis of GC. The levels of nesfatin-1 in 40 patients with GC and 40 healthy individuals, who were selected from the Chaohu Hospital Affiliated to Anhui Medical University, were assessed. ELISA was used for the measurement of plasma nesfatin-1 levels, while immunohistochemistry was applied to determine Ki67 protein expression in GC and normal gastric tissues. The diagnostic value of plasma nesfatin-1 for GC was further assessed using receiver operating characteristic (ROC) curve analysis. The results revealed that, compared with the controls, the mean nesfatin-1 levels in patients with GC were significantly increased. Furthermore, the protein expression of Ki67 in GC tissue was significantly upregulated compared with that in normal gastric tissue. Plasma nesfatin-1 levels were also demonstrated to be correlated with Ki67 protein expression in GC tissues. Additionally, ROC curve analysis indicated the potential diagnostic value of nesfatin-1, and the area under the ROC curve (AUC) for nesfatin-1 was 0.857 (95% confidence interval, 0.769-0.946). At a threshold nesfatin-1 level of 1.075 ng/ml, the optimal sensitivity and specificity were 70.0 and 95.0%, respectively, in discriminating patients with GC from healthy controls. These results indicated that plasma nesfatin-1 may serve as a novel biomarker for the diagnosis of GC and determination of GC cell proliferation.

KISS 1 and KISS 1R, a novel pair of metastasis suppressors, are likely to be associated with the prognosis of colorectal cancer ( CRC ). Here, a meta‐analysis was performed to study the role of KISS 1 and KISS 1R in CRC . Heterogeneity, stability and publication bias were all estimated. Six publications describing a total of 559 CRC patients were included in the present study. Low KISS 1 expression predicted 70% higher risk of poor prognosis for general patients ( HR , 1.71; 95% CI , 1.28–2.29) and 99% higher risk for East Asian patients ( HR , 1.99; 95% CI , 1.46–2.72). Limited evidence indicated that decreased KISS 1R expression might predict poor outcome ( HR , 2.96; 95% CI , 1.51–5.82). Neither heterogeneity nor publication bias was identified. The current analyses suggest that low KISS 1 expression predicts poor overall survival among East Asian patients with CRC . Evidence on other races and KISS 1R are still insufficient, and additional studies are required to clarify the risk of CRC associated with KISS 1R by race.

Objective To investigate the distribution of common deafness genes in 142 cases of hereditary hearing loss in Anhui Han population by gene chip technique.Methods Children's blood spot were collected and the DNA was extracted,then 9 mutations in GJB2,SLC26A4,GJB3 and mtDNA 12s rRNA deafness genes in the children were detected by using genetic deafness gene detection kit (microarray chip method).Results Of the 142 children with hereditary deafness,68 children with deafness-related gene mutations were detected (47.89％).The mutation rates of GJB2,GJB3,SLC26A4,mtDNA 12S rRNA were 28.17％ (40/142),0.70％ (1/142),18.31％ (26/142),5.63％ (8/142),respectively.Conclusion GJB2,SLC26A4,mtDNA 12S rRNA are the main genes leading to deafness in children with hereditary deafness in Anhui Han nationality,The GJB2 gene 235delC and SLC26A4 gene IVS7-2A ＞ G and mtDNA 12s rRNA gene 1555A ＞ G is the most common mutations of deafness gene in this study group.

Objective This study aimed to investigate whether the copy numbers of the CCL3L1 (Chemokine C-C-Motif Ligand 3 Like Protein 1) gene were associated with susceptibility to ankylosing spondylitis (AS). Methods A total of 806 Chinese individuals including 405 AS patients and 401 healthy controls were enrolled. The CCL3L1 gene copy number was measured by a custom-by-design Multiplex AccuCopyTM Kit based on a multiplex fluorescence competitive polymerase chain reaction (PCR) principle, and 50 samples were randomly selected using the fluorescent quantitative PCR method to verify copy number. Main statistical method was t test, chi-square test and logistic regression model. Results There were no statistically significant differences between the case group and control group in age and gender ( t=1.77, P=0.076, χ2=1.14, P=0.289). The copy number of CCL3L1 gene ranged from 0 to 13 in both AS patients and the controls. After copy numbers were classified into 3 categories by 3, we did not find significant difference between the two groups ( χ2=0.591, P=0.669). And regression analyses also did not support the hypothesis that CCL3L1 gene copy number variation (CNV) could be an impact factor to the severity or function indexes of AS patients ( χ2=0.341, P=0.804 and χ2=0.472, P=0.774, respectively). Conclusion We suggest that the copy number of the CCL3L1 gene does not have a role in the susceptibility and the severity or function to AS.

OBJECTIVE:To investigate the mechanism of delaying the senescence of human diploid fibroblast (2BS) by total flavone of Ginkgo biloba (FG).METHODS:The drug sera of FG was used to treat the 2BS. The population doublings of 2BS cells were observed, the mRNA expression of P16 gene was determined by fluorescence real-time quantitative PCR.RESULTS:FG significantly extended the population doublings of 2BS cells, and decreased the expression of P16 mRNA.CONCLUSION:FG can delay the senescence of cells by inhibiting the P16 gene expression.

The appropriate scaffold is one of the there major factors of tissue engineering.In this paper we discussed the application of natural scaffold materials including collagen,chitosan,HA,coral and silks in tissue engineering.

组织工程学是近年来发展起来的一门新学科,是材料学、工程学和生命科学共同发展并相互融合的产物,其最基本的思路是在体外分离、培养细胞,将一定量的细胞接种到具有一定空间结构的支架上,通过细胞之间的相互黏附、生长繁殖、分泌细胞外基质,从而形成具有一定结构和功能的组织或器官[1],因而,充足的种子细胞、合适的支架及促进种子细胞在支架上增殖分化的因子便成为组织工程的三大要素.