Objective To evaluate the application of COX-2 and other tumor markers in serum in diagnosing non-small cell lung cancer(NSCLC) and their correlation with the clinical stage of NSCLC.Methods Using the ELISA method and tumor markers protein chip,the serum levels of COX-2,CEA,CA125 and CA19-9 in 73 cases of NSCLC,21 cases of benign pulmonary disease and 16 cases of normal controls were measured and compared among the three groups of the cases.Results The concentrations of serum COX-2,CEA,CA125 and CA19-9 in the patients with NSCLC were significantly higher than those in the group with benign lung disease and in the control group(p < 0.01).The levels of COX-2 remarkably went up in NSCLC patients with increase in tumor size,lymph node metastasis and clinical stage development(p < 0.01).Conclusion serum levels of COX-2,CEA,CA125 and CA19-9 were much valuable in diagnosis of NSCLC.Furthermore,the serum COX-2 would also be a good marker for the invasion,metastasis and status of NSCLC.

目的研究过氧化苯甲酰摄入量与肝脏毒性作用关系.方法以wistar大鼠为研究对象,分组灌胃,连续8周.检测血清总胆红素(TBIL),丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST),碱性磷酸酶(ALP),测定肝组织中丙二醛(MDA),超氧化物歧化酶(SOD).做病理组织学检查.结果与对照组相比,各实验组肝组织中SOD活力下降.大剂量组大鼠的血清中TBIL,ALT,AST,ALP,肝组织中MDA量增高.病理学检查可见各实验组肝脏炎性表现.结论长期摄入过氧化苯甲酰对大鼠肝脏存在毒性作用.随剂量增加,毒性增加.

To explore an appropriate management for Paget's disease of the breast through analyzing the clinical manifestation,diagnosis and treatment.Mammary Paget's disease is a rare skin epithelial neoplasm.Because lack of clinician awareness of the disease,often caused by misdiagnosis, wrong treatment.In recent years,with the depth of its pathogenesis,the corresponding diagnosis, treatment,research has also made great progress.As a special type of breast cancer,Paget's disease has the features of low morbidity,higher misdiagnosis and longer survival with early diagnosis and proper treatment.

[Objective]To summarize the experience of laparoscopic cholecystectomy(LC),in order to reduce or prevent the complications.[Methods]The curative effects of 521 LC cases were analyzed retrospectively.[Results]520 cases of LC were successful,and one case was transfer to receive the laparotomy.[Conclusion]If doctors pay attention to the operation details and master the chance of laparotomy,the complications of LC can be reduced or prevented.

With the food industry's progress.Food additives are also increasingly being used by people,but their safety and more concern.Excessive intake of food additives will lead to some diseases.Worthy of general attention of medical researchers.Benzoyl peroxide(BPO) is now widely used additives Flour Enterprises,mainly used to bleach flour.However,their safety has always been widely questioned.However,the study of its toxicity has been reported rarely. In this paper,the pathogenicity of the current food additives research and the toxicity of benzoyl peroxide a comprehensive overview of research.

Objective To construct a mifepristone inducible recombinant adenovirus carrying enhanced green fluorecence protein for future gene therapy in vivo.Methods Full length red fluorescent gene and mifepristone regulator cassette was subcloned into PDC313 shuttle plasmid.The positive clone was identified by sequencing.Recombinant adenovirus were produced after cotransfection of the shutter vector PDC-EGFP and adenovirus DNA helper plasmid pBHGloxE1,3Cre into 293 cells.The recombined adenovirus was purified by CsCl density gradient centrifugation and its titer was determined by end point dilution assay.Fluorescence microscopy and FCM were confirmed by the activation of this regulatable recombinant adenovirus vector in infected cells.Results The adenovirus vector containing enhanced green fluorecence protein gene was identify by PCR.The virus titer of Ad-RUEGFP was 6.6×1010pfu/ml.Without the absence of mifepristone,no significant enhanced green fluorecence protein activation was observed,whereas in the presence of mifepristone activation of the enhanced green fluorecence protein was positive correlation with the inducer under definite range.Conclusion A replication-defective recombinant adenovirus vector containing enhanced green fluorecence protein was successfully constructed,which can be inducible by mifepristone.

<正>为探讨慢性结石性胆囊炎与肝脏损害程度的关系,对3年内因慢性结石性胆囊炎行择期胆囊切除术中60例<60岁患者术中取肝组织进行活检,现将结果分析报道如下。1临床资料1.1病例选择标准①60岁以下;②非急性发

Since the tumor research had entered intomolecular era,the scientific workers have provided some scientific basis and the possibility for illuminating the relationship between the alternation of structure,expression,regulation,function of genes and tumor formation.The research on tumor suppressor gene of breast cancer is benefit to improve the study for the reason of breast cancer,the occurrence,the development and prognosis,which is a new way for breast cancer examination and cure.