目的：探讨Fas及FasL在滋养细胞、正常葡萄胎、侵袭性葡萄胎、绒癌中的表达及意义。方法：利用免疫组织化学方法检测Fas及FasL蛋白在正常滋养细胞(40例)、完全性葡萄胎(36例)、侵袭性葡萄胎(20例)、绒癌(20例)中的表达；用逆转录PCR(RT-PCR)检测Fas、FasL基因在4组组织中的表达。结果：免疫组织化学方法显示Fas蛋白在正常滋养细胞组、完全性葡萄胎组、侵蚀性葡萄胎组和绒癌组的阳性表达率分别为90%（36例）、86.1%（31例）、40%（8例）、30%（6例），FasL蛋白在正常滋养细胞组、完全性葡萄胎组、侵蚀性葡萄胎组和绒癌组的阳性表达率分别为17.5%（7例）,27.8%（10例）、55%（11例）、75%（15例）。在侵蚀性葡萄胎和绒癌中Fas蛋白低表达、FasL蛋白高表达；PCR结果显示Fas基因在正常滋养细胞组、完全性葡萄胎组、侵蚀性葡萄胎组、绒癌组中表达量逐渐降低，而FasL基因表达量逐渐升高。结论：在侵蚀性葡萄胎及绒癌中Fas低表达及FasL高表达，这与侵蚀性葡萄胎及绒癌的凋亡机制密切相关。

目的 探讨雌激素和雌、孕激素联合应用所致大鼠子宫肌瘤后的免疫功能变化及其机制.方法 选取SPF级雌性SD大鼠90只,编号后采用随机数字表法分为空白组、雌激素组和雌+孕激素组各30只,采用苯甲酸雌二醇对雌激素组和雌+孕激素组进行肌肉注射连续3个月(0.2 mg/只,3次/周),雌+孕激素组在造模3个月后加用黄体 酮1.5 mg/只肌注(2次/周,连续1个月).观察比较三组大鼠子宫组织平滑肌厚度、子宫重量、卵巢重量,以及E2水平、ER评分、DR评分,测定T细胞亚群.结果 子宫组织平滑肌厚度值雌+孕激素组＞雌激素组＞空白组且组间差异均具有统计学意义(P＜0.05).雌+孕激素、雌激素组大鼠的子宫重量在造模第3个月、4个月较空白组显著的增加(P＜0.05)、卵巢重量较空白组显著的降低(P＜0.05).雌+孕激素、雌激素组大鼠的E2水平、ER评分、PR评分显著高于空白组(P＜0.05),雌+孕激素组的E2水平、ER评分、PR评分显著高于雌激素组(P＜0.05).雌+孕激素、雌激素组大鼠的CD3+、CD4+、CD4+/CD8+水平显著低于空白组(P＜0.05)、CD8+显著高于空白组(P＜0.05),雌+孕激素组的CD3+、CD4+、CD4+/CD8+水平显著低于雌激素组(P＜0.05).结论 雌激素和雌、孕激素联合应用所致大鼠子宫肌瘤模型免疫功能显著低下,激素受体水平可能是其主要影响因素之一.

Objective To investigate the effect of total trans retinoic A acid(ATRA) on the occurrence and development of uterine leiomyoma in the rat model of estrogen and progesterone induced uterine leiomyoma.Methods Sixty female Wistar rats of SPF grade,numbered by the random number table method were divided into blank group,model group and ATRA group,20 rats in each group. The model group and ATRA group were given estradiol benzoate [0.5mg/(kg·d),intramuscular injection, 12weeks]and progesterone [5.9mg/(kg·d) intramuscular injection,12weeks];ATRA group was also given ATRA at week 10 (0.5mg/d,2weeks).Analysis of each rat uterine weight and uterine coefficient index,and serum hormone,dimensional a acid receptor alpha(RAR alpha),nitric oxide synthase(iNOS)level.Results The weight of uterus,uterus coefficient,left and right uterine horn length value, cornual maximum values of the model group and ATRA group were significantly higher than those of the blank group(P<0.05). The weight of uterus, uterus coefficient,left and right uterine horn length value,cornual maximum, cervical length value of the ATRA group were significantly lower than those of the model group(P<0.05).The estradiol(E2), progesterone(P),luteinizing hormone(LH) values in ATRA group were significantly lower than those in model group(P<0.05). The retinoic acid receptor alpha(RARα)expression level of the model group and ATRA group were significantly higher than that of the blank group(P<0.05). The iNOS expression levels were higher than those of the blank group and ATRA group (P<0.05). The iNOS expression level in the uterine tissue of the ATRA group was significantly higher than that of the blank group(P<0.05).Conclusion All trans retinoic acid can be accomplished by inhibition of no increase in conjunction with RAR alpha,regulate hormone levels,so as to achieve purpose of inhibiting the proliferation of uterine smooth muscle cells.

<正>宫颈癌放疗后,易引起阴道放射性炎症改变。尤其是中、老年患者宫颈放疗后,阴道炎症出现早、症状重,治疗困难,给患者带来痛苦,生活质量下降。本研究对中老年患者宫颈癌放疗后阴道炎采用常规阴道冲洗加复方甲硝唑栓(内含甲硝唑及人参茎叶苷、维生素E)联合结合雌激素软膏(倍美力软膏)局部用药治疗,取得良好疗效,现报道如下。