Purpose: The long noncoding RNA RGMB-AS1 plays an important part in the genesis and progression of multiple human cancers. Nonetheless, little is known regarding its expression, roles, and mechanisms of action in gastric cancer (GC). This study was aimed at investigating the relationship between RGMB-AS1 and GC and illustrating the mechanisms of action of RGMB-AS1 therein. Methods: RGMB-AS1 expression in GC was measured via reverse-transcription quantitative PCR. A series of experiments including Cell Counting Kit-8 assay, flow-cytometric analysis of apoptosis, Transwell migration and invasion assays, and in vivo tumorigenesis experiment were conducted to test the effects of RGMB-AS1 on the malignant phenotype of GC cells. The molecular events behind the oncogenic actions of RGMB-AS1 in GC were elucidated through subcellular fractionation, RNA immunoprecipitation assay, bioinformatics analysis and luciferase reporter assay. Results: RGMB-AS1 upregulation was confirmed in GC tissues and cell lines. Higher RGMB-AS1 expression was associated with adverse clinical parameters and negatively correlated with patient overall survival. RGMB-AS1 knockdown inhibited GC cell proliferation, facilitated apoptosis, and reduced migration and invasion in vitro. Further experiments revealed that RGMB-AS1 knockdown decreased the tumor growth of GC cells in vivo. Mechanistically, RGMB-AS1 functioned as a competing endogenous RNA upregulating histone deacetylase 4 (HDAC4) by sponging microRNA-574 (miR-574). Rescue experiments indicated that miR-574 inhibition and HDAC4 reintroduction reversed the effects of the RGMB-AS1 knockdown on GC cells. Conclusion: The RGMB-AS1-miR-574-HDAC4 regulatory network contributes to the malignancy of GC, thereby offering a novel target for the diagnosis, prognosis, and/or treatment of GC.

内镜下全层切除术(endoscopic full-thickness resection,EFR)治疗胃肠间质瘤,治疗成功的关键是内镜下成功修补术中发生的迟发性穿孔.本例患者胃间质瘤(gastric stromal tumor,GST)行EFR治疗,术后患者恢复良好,术后3个月并发膈下脓肿,现报道如下.

双氯芬酸钠因具有解热、消炎、镇痛等作用,被广泛用于临床,其不良反应研究多以胃十二指肠损害为主,而下消化道损伤报道较少.现报道本院发现的双氯酚酸钠致消化道溃疡2例,以提高临床医生的认识. 1 病例资料 病例1:患者男,56岁,因反复乏力、头晕4年于2016年11月22日入本院.多次在当地医院检查发现贫血,Hb波动于60 ～ 80 g/L,最低至25 g/L,多次便隐血(+).2016年3月5日当地医院胃镜示:胃体、胃窦陈旧出血点及糜烂,胃窦小弯侧一凹陷性溃疡,表面覆白苔及出血,胃窦前壁大片糜烂.2016年3月11日结肠镜示:乙状结肠、横结肠散在数个大小不一溃疡,部分溃疡见新鲜出血.

<正>1临床资料患者女,62岁,发现脾占位3天入院。4周前无明显诱因出现呼吸困难,1周前就诊于我院呼吸内科,超声诊断为胸腔积液,给予穿刺置管引流。入院前超声回报脾肿大伴多发肿物,胸部CT回报脾脏体积增大,实质内可见圆形软组织密度影,其内密度不均匀。以脾占位转入我院普外科,既往史健康,无结核及肝炎病史,未见明显阳性体征。

1一般资料患者男,49岁,吉林省伊通县河源镇新生村人。2010年8月7日入院,主诉中上腹部阵发性钝痛伴腹胀1年,加重1个月。该患于1年前无明显诱因出现上腹部偏左侧阵发性锐痛,持续时间约1-2个小时,无放散痛,无明显加重及减弱趋势,无恶心、呕吐,无呕血及咖啡样物,后腹部疼痛逐渐消失。入院时患者即一般状态良好,既往史健康。入院体格检