目的:探讨DNMT3L(DNA methyltransferase 3-like)蛋白在不同种类卵巢恶性生殖细胞肿瘤中的表达及其在生殖细胞肿瘤发生发展过程中的作用.方法:收集卵巢恶性生殖细胞肿瘤59例,包括原始生殖细胞肿瘤52例及未成熟性畸胎瘤7例,原始生殖细胞肿瘤包括胚胎性癌10例,卵黄囊瘤22例,无性细胞瘤13例,混合性生殖细胞肿瘤7例;同时收集非生殖细胞肿瘤17例,包括浆液性癌5例,颗粒细胞瘤5例,卵泡膜细胞瘤7例,采用SP免疫组织化学方法检测肿瘤组织中的DNMT3L蛋白的表达情况,并结合临床病理特征及组织学类型进行统计学分析.结果:DNMT3L主要在原始生殖细胞肿瘤表达,总体阳性率为73.1％(38/52),其中混合性生殖细胞肿瘤阳性表达率为85.7％(6/7),无性细胞瘤阳性表达率为84.6％(11/13),卵黄囊瘤中阳性表达率为68.2％(15/22),胚胎性癌阳性表达率为60％(6/10).DNMT3L在7例未成熟畸胎瘤及17例非生殖细胞肿瘤中均未见阳性表达.临床病理特征分析显示DNMT3L阳性表达率与原始生殖细胞肿瘤的FIGO分期有关,与年龄及肿瘤大小等无关.结论:DNMT3L在原始生殖细胞肿瘤阳性表达率高,对其诊断具有较好的敏感性和特异性,DNA甲基化的改变可能在卵巢原始生殖细胞肿瘤的发生过程中起到重要作用.

患儿女,10个月,无明显诱因发现枕骨后隆起4个月入院,无特殊外伤史,随访观察数月,枕骨后隆起增大. 行颅脑MIR平扫+DWI成像示右侧枕骨骨质局限性破坏,相应区域可见软组织肿块影,呈等T1稍长T2信号影,FLAIR为稍低信号, DWI 为等信号,范围22 mm × 11 mm × 22 mm (图1),考虑朗格汉斯组织细胞增生症可能性大.

Objective:To investigate the expression and relationship of SUMO specific protease 1 (SENP1) and ERa in type-1 endometrial carcinoma.Methods:Fifty endometrial cancer cases were involved in this study.Immunohistochemical method was performed to study SENP1 protein and estrogen receptor (ER) expression in endometrial cancer tissues and para-carcinoma tissues.Correlations between SENP1 and ERα,as well as clinical pathological parameters were also analyzed.Results:Expression of SENP1 protein in type-1 endometrial carcinoma was significantly higher than that in the para-cancer tissues.Expression of SENP1 protein was significantly higher in well-differentiated tumors than in poorly-differentiated tumors.Expression of SENP1 was positively correlated with the expression of ERα,but not with the age of patients,the depth of tumor invasion,tumor stage and lymph node metastasis.Conclusion:SENP1 may play an important role in the occurrence and development of type-1 endometrial carcinoma,which may be a new target for the treatment of endometrial carcinoma.

Objective To determine the significance of MDR -1 and MRP mRNA expression in periph-eral blood lymphocytes in lung cancer patients with chemotherapy treatment .Methods Peripheral blood samples were taken from each lung cancer patient before chemotherapy and three weeks after the first chemotherapy cycle . The expressions of MDR-1 and MRP were tested for 39 lung cancer cases using RT -PCR technique comparing to 20 normal control cases .Results There was no significant difference for MDR -1 mRNA and MRP mRNA ex-isting in lung cancer cases and control group (P>0.05).MDR-1 and MRP mRNA expressions were increased after treatment of chemotherapy courses in almost all pathological types of lung cancer .Furthermore,the expres-sion level in small cell lung cancer after chemotherapy was significantly higher than that before .Conclusion Chemotherapy may induce the incidence rate of MDR -1mRNA and MRP mRNA expression in lung cancer .Ade-nocarcinoma and squamous cell carcinoma are mainly intrinsic MDR while small cell lung cancer is mainly ac -quired MDR to chemotherapy .Based on the positive expression rate of MDR -1 mRNA and MRP mRNA in pe-ripheral blood ,we may evaluate the results of chemotherapy in lung cancer patients easily and conveniently .

病人,女,32岁;因孕26+2周,下腹痛4d入院.查体:病人有男性化体征.专科检查:右侧子宫旁可扪及一直径约10 cm包块,质较硬,活动,无压痛.产科B超检查示:中期妊娠,单活胎,子宫右侧低回声团,腹水(少量).入院后在硬膜外麻醉下行右卵巢剥除术,术中见:右卵巢内囊实性包块,约11 cm×10 cm×10 cm大小,粘连于侧腹膜,范围约5 cm×5 cm大小,蒂部无扭转,右侧输卵管系膜见囊性包块,约3 cm×3 cm大小,其内为清亮液体,子宫直肠凹内可见淡黄色游离液体约300 mL.病理检查大体观:灰白结节样包块一个,大小12.0 cm×9.0 cm×4.5 cm,包膜完整,切面灰白,质稍脆,部分区域血管丰富,灰红出血.镜检:增生的卵泡膜黄素细胞呈脑回样排列或实性生长,大小形态较一致,圆形肥硕,细胞核位于中央,嗜酸性胞浆内可见大小不一的空泡和小滴(图1).免疫组化:α-inhibin(+)、calretinin(+)、CD99(+)、PLAP( - )、HMB45(-)、SMA(- )、CK(-)、EMA(-)、血管CD34(+)(图2).病理诊断:右侧卵巢妊娠黄体瘤(大小12.0 cm×9.0 cm×4.5 cm),部分区域伴坏死.

目的探讨影响老年小细胞肺癌预后的各种相关因素。方法回顾性分析22例老年小细胞肺癌患者的临床资料。结果小细胞肺癌患者总体中位总生存时间8.0个月,<65岁组10个月、≥65岁组5.5个月(P=0.085),≥65岁治疗组中位总生存8.0个月、非治疗组3.0个月(P=0.007),<65岁治疗组中位总生存10个月、非治疗组5个月(P=0.007),<65岁治疗组与≥65岁治疗组中位总生存时间比较P=0.178;查尔森合并症指数(CCI)≥2组中位生存时间为3.0个月,CCI=1/0组中位生存时间为8.0个月,P=0.001。多因素分析显示,化疗或化疗联合放疗及CCI<2是老年小细胞肺癌患者预后良好的独立因素。结论老年小细胞肺癌患者的生存与能否治疗和CCI评分明显相关。

OBJECTIVE: To establish a method for detecting the expressions of OATP-B and OATP-DmRNA in lung cancers via real-time FQ-RT-PCR and to analysis their relerationship with the clinical data in order to evaluate the clinical significance.METHODS: The expressions of OATP-B and OATP-DmRNA in 40 primary lung cancers and 10 normal lung tissues were detected by SYBRGreen quantitative real-time PCR.The expression differences of OATP-B and OATP-DmRNA between lung cancers and normal lung tissues were studied,and the relerationship between the expressions and their clinical data was also analyzed by the statistical method.RESULTS: The expressions of OATP-B and OATP-DmRNA were all signicantly up-regulated in the lung cancers compared with those in the normal lung tissues(P0.05).There was also a significant difference in the expressions of OATP-B and OATP-DmRNA between lymph metastasis lung cancers and those without lymph metastasis(P0.05).CONCLUSION: Compared with normal lung tissues,the expressions of OATP-B and OATP-DmRNA are all significantly up-regulated in lung cancers,and the expression level in lymph metastasis lung cancers is higher than that without lymph metastasis.

Objective To investigate the expression and clinical significance of organic anion transporting polypeptide(OATP)-B,D mRNA and protein in lung cancer.Methods The expressions of OATP-B and OATP-D mRNA were detected in 40 cases of lung cancer and 10 cases normal lung tissues by SYBRGreen Quantitative Real-time PCR,as well as the expressions of OATP-B and OATP-D were available by immunohistochemistry analysis.Results The expressions of OATP-B and OATP-D mRNA were all significantly up-regulated in different pathological category lung neoplasms compared to normal lung tissues.There was also a significant difference in the expressions of OATP-B and OATP-D mRNA between lymph metastasis lung neoplasms and those without lymph metastasis,P0.05.Immunohistochemistry analysis showed a higher expression of OATP-B and OATP-D protein in lung cancers than normal lung tissues,as well as in lung cancers with lymph metastasis compared to those without lymph metastasis(P0.05).Conclusion The expression of OATP-B and OATP-D mRNA and protein raise in lung cancer.Both of them are associated with lymph metastasis.

目的 探讨人类有机阴离子转运多肽(OATP)家族中的OATP-B和OATP-D的mRNA及其蛋白表达和肺癌的关系.方法 采用实时荧光定量PCR(FQ-PCR)法检测江苏省淮安市第一人民医院和青岛市立医院2004-2006年收集的40例肺癌组织和10例正常肺组织中OATP-B和OATP-D的mRNA的含量;免疫组织化学法检测40例肺癌组织和20例癌旁组织中OATP表达.结果 OATP-B和OATP-D的mRNA及蛋白表达在肺癌组织中表达均上调,与正常肺组织比较,差异有统计学意义(P<0.05).各种病理类型之间比较,差异无统计学意义(P>0.05).有淋巴结转移的肺癌组织中OATP-B和OATP-D的mRNA及蛋白表达上调与无淋巴结转移的肺癌组织比较,差异有统计学意义(P<0.05).OATP-B和OATP-D的mRNA及蛋白表达与肿瘤直径无关.结论 各组织类型肺癌与OATP-B和OATP-D的表达相关,与淋巴结转移相关.OATP-B和OATP-D的表达异常与肿瘤的大小、性别及年龄无关.

Objective To establish a fluorogenic quantitative RT-PCR(FQ-RT-PCR)method for determining expressions of OATP-B and OATP-DmRNA,and to investigate expressions of OATP-B and OATP-DmRNA in lung neoplasms and nrmal lung tissues.Methods The OATP PCR product was sub-cloned into the PGEM-T vector to construct recombinant plasmids.After optimizing the thermocycling conditions,a quantitative RT-PCR assay was successfully developed to quantify OATP expression on the Line-Gene real-time PCR detection system,which was confirmed by 40 samples of lung cancer and normal lung tissues.Results The standard curve was successfully established,and the linear regression value was 0.998.Using FQ-RT-PCR,we can get an accurate quantitative result of the OATP-B and OATP-DmRNA expressions.Moreover,expression levels of OATP-B and OATP-DmRNA in lung neoplasms were higher than those in normal lung tissues.Conclusion FQ-RT-PCR for detecting expressions of OATP-B and OATP-DmRNA was sucessfully established,and is accurate and sensible.