This study aims to evaluate the impact of gynecologic oncologists' laparoscopic proficiency on survival outcomes in cervical cancer patients. A cohort of 1,965 cervical cancer cases from four clinical centers in China was analyzed, including abdominal radical hysterectomy (ARH), laparoscopic radical hysterectomy (LRH), and robotic radical hysterectomy (RRH). The median operative time (MOT) for LRH was used as a measure of surgical proficiency. Survival outcomes of ARH vs. LRH were compared in early-stage cervical cancer patients without adjuvant therapy to identify a critical MOT threshold. Below this threshold, no significant differences in prognosis were observed between ARH and LRH. Propensity score matching and mixed-effects Cox regression were used to adjust for baseline risk factors and random effects, validating the finding across all LRH cases. The Kaplan–Meier analysis showed that improved prognosis was associated with reduced MOT. When gynecologic oncologists had an MOT within 210 min, LRH vs ARH was no longer a significant risk factor (HR 1.1998; 95

As one of the three major treatments for cervical cancer, radiotherapy plays an important role in treating cervical cancer. In recent years, new technologies such as three-dimensional external beam radiotherapy and image-based brachytherapy have been widely used in the treatment of cervical cancer, which can increase the dose of tumor radiation while reducing the dose radiating surrounding normal tissues. In addition, the researches on the use of individualized radiotherapy guided by imaging indicators and proton therapy are also in progress. This paper presents the basic knowledge of radiotherapy for cervical cancer and the application of radiotherapy in the treatment of cervical cancer, summarizes the application of new technologies, and proposes issues that need to be emphasized in the use of new technologies, in order to guide clinical application.

目的 探讨手术分期与影像学分期对局部晚期宫颈癌患者放疗预后的影响及影像学评估淋巴结转移的准确性.方法 收集2015年5月至2017年11月在首都医科大学附属北京妇产医院妇瘤科接受手术分期及放疗的23例ⅠB2～ⅢB期宫颈鳞状细胞癌患者的病例资料及随访结果,随机选取同期进行影像学分期及放疗且资料完整的46例宫颈鳞状细胞癌患者作为对照,根据2019年国际妇产科联盟分期标准再次分期,对比两种分期方法对放疗及预后的影响,评估CT/磁共振成像(MRI)判断淋巴结转移的准确性.结果 从疾病诊断至治疗结束,手术分期组时间(77±6)d,影像学分期组时间(69±5)d;手术分期组手术至放疗开始时间(8.4±1.8)d.手术分期组放疗后下肢水肿发生率高于影像学分期组[43.5％(10/23)比8.7％(4/46)](χ2=11.470,P=0.001).与术后病理结果对比,手术分期组术前CT/MRI评估淋巴结转移的灵敏度为71.4％(10/14),特异度为81.3％(13/16),有4例患者根据术后病理结果分期升级,3例患者分期降级.随访时间8～40个月,平均(31±8)个月,手术分期组3年无进展生存率为78.3％,影像学分期组为51.9％,两组比较差异有统计学意义(P<0.05);手术分期组3年总生存率为81.8％,影像学分期为66.2％,两组比较差异无统计学意义(P>0.05).结论 与影像学分期相比,手术分期能够得到更准确的淋巴结转移结果,从而决定放射剂量及放射野,根据手术分期指导放疗能明显降低宫颈癌复发率.

目的 探索注射用氨磷汀对宫颈癌同步放化疗患者的保护作用.方法 选取2017年3月至2018年3月首都医科大学附属北京妇产医院妇瘤科收治的经病理组织学检查确诊的57例宫颈癌患者为研究对象,随机分为对照组(28例)和试验组(29例).两组均施行放疗同步顺铂化疗,试验组放疗前30 min予氨磷汀静脉滴注.观察两组同步放化疗完成情况以及不良反应发生情况.结果 两组宫颈癌患者一般情况及同步放化疗完成情况比较差异无统计学意义(P>0.05).试验组与对照组恶心呕吐≥G2、急性期腹泻≥G2的发生率比较差异无统计学意义(P>0.05).试验组急性期骨髓抑制发生率明显低于对照组[31.0％(9/29)比57.1％(16/28)](P<0.05),且试验组白细胞计数最低值高于对照组[(2.9±1.5)×109/L比(2.1±1.4)×109/L](P<0.05);但两组血红蛋白及血小板计数最低值比较差异无统计学意义(P>0.05).两组急性期皮肤反应≥G2的发生率比较差异无统计学意义(P>0.05),且两组均未发生3级及以上皮肤不良反应.结论 在宫颈癌同步放化疗过程中,静脉输注氨磷汀可有效降低急性期血液学毒性,保证治疗进程,并有降低急性期腹泻发生率的趋势,但上消化道不良反应发生率可能轻度升高.

Aim: To discuss F11-AS1's effects and mechanisms in ovarian cancer development. Methods: Evaluating F11-AS1 expression by ISH assay and F11-AS1 mRNA level in difference cell lines by RT-qPCR assay. Using MTT, flow cytometry, transwell and wound healing assay to evaluate SKOV3 cell proliferation, cell apoptosis, invasion and migration. And using WB assay to measure PTEN, p-PI3K, AKT, P53 and MMP-9 proteins expressions. Results: F11-AS1 was significantly down-regulation with stage increasing in cancer tissues (P < 0.01, respectively). With F11-AS1 transfection, the SKOV3 cell proliferation rate was significantly depressed with cell apoptosis and G1 phase rate significantly increasing (P < 0.001, respectively). And then, invasion cell number and wound healing rate of lncRNA group which transfected with F11-AS1 significantly down-regulation (P < 0.001). By WB assay, PTEN and P53 proteins expressions significantly up-regulation and p-PI3K, AKT and MMP-9 proteins expressions were significantly down-regulation (P < 0.001). Conclusion: F11-AS1 depresses ovarian cancer biological activity by regulating PTEN by vitro study.

Several microRNAs (miRs) play regulatory roles in cervical cancer. The present study investigated the molecular role of microRNA-29a in cervical cancer via modulation of cell division cycle 42 (CDC42) expression. The results showed significant (P < 0.05) downregulation of miR-29a human in cervical cancer cells. Overexpression of miR-29a inhibited the proliferation and colony formation of the cervical cancer cells via G0/G1 cell cycle arrest. In silico analysis and luciferase reporter assay showed CDC42 to be the molecular target of miR-29a. CDC42 was found to significantly (P < 0.05) overexpressed in cervical cancer cells. Nonetheless, miR-29 overexpression inhibited the expression of CDC42 in DoTc2 cells. Silencing of CDC42 inhibited the proliferation of DoTc2 cells. However, overexpression of CDC42 could prevent the tumour-suppressive effects of miR-29a. Finally, miR-29a was found to suppress the tumour-growth in vivo further confirming the tumour-suppressive effects of miR-29. Collectively, the findings of the present study revealed the tumour-suppressive effects of miR-29 of cervical cancer.

Background Radiosensitivity is limited in cervical cancer (CC) patients due to acquired radiation resistance. In our previous studies, we found that immediate-early response 5 (IER5) is upregulated in CC cells upon radiation exposure and decreases cell survival by promoting apoptosis. The details on the transcriptional regulation of radiation-induced IER5 expression are unknown. Studies in recent years have suggested that Pol II-associated factor 1 (PAF1) is a pivotal transcription factor for certain genes “induced” during tumor progression. In this study, we investigated the role of PAF1 in regulating IER5 expression during CC radiotherapy. Methods PAF1 expression in CC cells was measured by western blotting, immunohistochemistry, and qRT-PCR, and the localization of PAF1 and IER5 was determined by immunofluorescence. The effect of PAF1 and IER5 knockdown by siRNA in Siha and Hela cells was studied by western blotting, qRT-PCR, CCK-8 assay, and flow cytometry. The physical interaction of PAF1 with the IER5 promoter and enhancers was confirmed using chromatin immunoprecipitation and qPCR with or without enhancers knockout by CRISPR/Cas9. Results We confirmed that PAF1 was highly expressed in CC cells and that relatively low expression of IER5 was observed in cells with highly expressed PAF1 in the nucleus. PAF1 knockdown in Siha and Hela cells was associated with increased expression of IER5, reduced cell viability and higher apoptosis rate in response to radiation exposure, while simultaneous PAF1 and IER5 knockdown had little effect on the proportion of apoptotic cells. We also found that PAF1 hindered the transcription of IER5 by promoting Pol II pausing at the promoter-proximal region, which was primarily due to the binding of PAF1 at the enhancers. Conclusions PAF1 reduces CC radiosensitivity by inhibiting IER5 transcription, at least in part by regulating its enhancers. PAF1 might be a potential therapeutic target for overcoming radiation resistance in CC patients.

目的 探讨子宫切除术后高级别阴道上皮内瘤变(HG-VAIN)行高剂量率腔内近距离治疗(HDR-ICR)的临床效果和不良反应.方法 回顾性分析13例HG-VAIN患者的病历资料,其中VAINⅡ1例,VAINⅢ12例,所有患者均仅接受HDR-ICR,选择阴道圆柱体和(或)阴道卵圆体施源器治疗.所有患者均进行随访,观察患者的复发与生存情况及近、远期并发症发生情况.结果 13例HG-VAIN患者的随访时间为12～117个月,中位随访时间为55个月;1例患者于治疗后22个月复发,经手术治疗后得到有效控制,治疗总有效率为92.3％(12/13).13例HG-VAIN患者中,3例患者出现近期不良反应,其中1～2级直肠反应1例,1级泌尿系统不良反应2例,且近期不良反应均较轻;6例患者出现远期不良反应,其中2级直肠反应3例,3级泌尿系统不良反应2例,3～4级阴道狭窄和溃疡1例.结论 HDR-ICR对子宫切除术后HG-VAIN患者是一种非常有效的治疗方法,治愈率高,且近期和远期不良反应可耐受.

目的 掌握后装γ源近距离治疗室(简称后装治疗室)内辐射场剂量水平及其分布,为机房的防护设计和改造以及现场人员的放射防护指导提供科学决策.方法 以北京市某医院1台RL-HZJ 18型192Ir后装γ源近距离治疗机(后装机)及其治疗室为研究对象,在实际放射源活度为254.7 GBq的情况下,使用AT1121型辐射巡测仪分别测量后装机贮源状态下治疗室内辐射场剂量水平和出束状态下迷路的剂量分布;使用热释光剂量计(TLD)测量患者治疗期间治疗室内关注点的辐射剂量水平.结果 后装机贮源状态下距离设备表面5和100 cm处的周围剂量当量率最高值分别为24.0和0.56 μSv/h;照射状态下迷路拐口周围剂量当量率为5.4×103 μSv/h,相应的辐射比率为3.8×10-2,迷路内接近入口防护门处为9.4 μSv/h,相应的辐射比率为6.8×10-5;患者治疗期间治疗室内墙面关注点的辐射比率为(0.235～201.4)×10-6.结论 后装治疗室迷路具有明显的防护效果,迷路的设置和治疗室内辐射剂量水平的实时监测是必要的.

Objective To analyze the therapeutic effect and adverse effect of salvage radiotherapy for occult invasive cervical cancer ( OICC) after simple hysterectomy .Methods Data of 15 OICC patients admitted to Beijing Obstetrics and Gynecology Hospital between Jan .2003 and Jan.2016 were retrospectively reviewed .All patients were staged ⅠA2 toⅡA, of which the number of patients in International Federation of Aynecology and Bbstetrics stage ⅠA2,ⅠB1,ⅠB2,andⅡA were 4,9,1,and 1,respectively.All the patients received regular external beam radiotherapy and /or brachytherapy fol-lowing simple hysterectomy .10 patients received both two aforementioned radiation treatments , and the other 5 patients received pelvic external beam radiotherapy .Of these 15 patients,8 patients underwent concurrent chemotherapy .All patients were followed up to January 2017.Recurrence,disease-free survival and short/long-term complications were investigated . Results ①Recurrence and survival:the follow-up ranges from 12 to 125 months.Median total survival for all 15 patients was 58 months, and median disease-free survival was 50 months.2 patients recurred in 9-18 months after therapy and died from tumor multi-metastasis.②Short/long-term complication:10 patients suffered short-term complication,including stageⅠ-Ⅱrectum toxicity(7 cases) and stage Ⅰ urinary bladder toxicity (3 cases),while 5 patients had long-term complica-tion,including stage Ⅰ-Ⅲrectum toxicity(3 cases) and stage Ⅱurinary bladder toxicity(2 cases).Conclusion Regular external beam radiotherapy and brachytherapy could be safe and effective salvage treatments with acceptable short /long-term complication for the patients with OICC .

AIM:Accumulating studies have revealed that microRNA (miR)-93 may exert an oncogenic role in various cancers via inhibiting CDKN1A. However, the involvement of miR-93/CDKN1A axis in cervical cancer remains unclear. We aimed to investigate expression pattern, clinical significance and potential functions of miR-93/CDKN1A axis in cervical cancer.METHODS:Expression levels of miR-93 and CDKN1A mRNA in 100 pairs of cervical cancer and matched non-cancerous tissue samples were detected by quantitative-PCR. Statistical analyses were performed to evaluate associations of miR-93 and/or CDKN1A expression with various clinicopathologic features and patients' prognosis. The functions of miR-93/CDKN1A axis on cell proliferation and invasion were also examined.RESULTS:Compared to non-cancerous tissues, the expression levels of miR-93 and CDKN1A were dramatically increased and decreased in cervical cancer tissues, respectively (both P < 0.01). High miR-93 and/or low CDKN1A expression were significantly associated with advanced International Federation of Gynecology and Obstetrics stage, the presence of lymph node metastasis and recurrence (all P < 0.05). Importantly, patients with high miR-93 and/or low CDKN1A expression had shorter overall survival than those with low miR-93 and/or high CDKN1A expression. The multivariate analysis identified miR-93 and/or CDKN1A expression as independent prognostic factors of cervical cancer. Functionally, miR-93 promoted cell proliferation and invasion of cervical cancer cells via inhibiting CDKN1A.CONCLUSION:miR-93 upregulation and CDKN1A downregulation may be both associated with the development, progression and patients' prognosis of cervical cancer. miR-93/CDKN1A axis may also play an important role in the malignancy of cervical cancer cells, suggesting its potential as a therapeutic target for this cancer.

Objective: The study aimed to evaluate the incidence of early and late radiation enteritis in cervical cancer patients receiving definitive or adjuvant radiotherapy (RT). Materials and Methods: Three hundred and twenty-four cervical cancer patients receiving definite or adjuvant RT in our hospital from January 2010 to December 2012 were divided into definitive (132 patients) or adjuvant RT (192 patients) and performed detailed analysis. Results: Early radiation enteritis was found in 54.3%, and late radiation enteritis was found in 17.9% of cervical patients. Early (P < 0.007) and late (P < 0.003) radiation enteritis appeared more frequently in patients treated with definitive RT than that with adjuvant RT. In the definitive RT group, incidence of both early and late radiation enteritis in the RT group was higher than in the radiochemotherapy (RCHT) group, and the difference was statistically significant (P < 0.004). Severe cases of late radiation enteritis (Grade 3 and 4) can be seen higher in the definitive radiation group (both the RT and RCHT group) than in the adjuvant radiation group, and the difference was statistically significant (P < 0.005). Conclusion: The incidence of both early and late radiation enteritis in the definitive RT group is higher than in the adjuvant RT group. The occurrence of side effects was associated with the prolongation of total irradiation time due to necessary interruptions of RT. Methods to decrease the interruptions in the RT and the irradiated volume of the small bowel will further lessen enteric morbidity.

[Objective] To assess the efficacy of radiotherapy alone in the treatment of endometrial adenocarcinoma and influencing factors.[Methods] Eighty-three patients with endometrial adenocarcinoma receiving radiotherapy alone were analyzed retrospectively.Patients underwent Iridium192 intracavitary treatment and Cobalt-60 external irradiation.Point A and point F were chosen as the dose reference points in intracavitary treatment.The association of efficacy with age,family history,gravidity and parity history,body mass index,clinical stages,histological grades,and depth of uterine cavity was analyzed by univariate and multivariate regression.Kaplan-Meier method was used to calculate the 5-year survival rate,and chi-square test on relevant factors was conducted to compare the difference in 5-year survival.[Results] The 5-year survival of 83 patients with endometrial cancer was 51.8％,and that of stage Ⅰ,Ⅱ,and Ⅲ patients was 70.8％,60.6％ and 23.1％,respectively.The prognosis of patients was significantly correlated with clinical stage,histological grad,and depth of uterine cavity (cut-off point:8cm) (P＜0.05).[Conclusions] Radiotherapy alone is safe and effective for endometrial cancer,especially for stage Ⅰ,Ⅱ,and Ⅲ patients who could not tolerate surgical treatment.Point A and point F can be applied as dose reference points in intracavitary treatment for endometrial cancer.

OBJECTIVE:The purpose of this study was to evaluate the clinical efficacy of preoperative vaginal intracavitary irradiation for Stage Ib2 and IIa cervical cancer.MATERIALS AND METHODS:From June 2008 to June 2014, data from 78 Stage Ib2 and IIa cervical cancer patients (age ≤75 years) with a diameter of local lesions >4 cm were collected in our hospital. Before treatment, all cases were confirmed by biopsy. The patients' general state was good, Karnofsky Performance Score ≥90, heart and lung functioning was normal, and patients were able to tolerate the surgery. The 78 patients were randomly divided into two groups: neoadjuvant radiotherapy group (NRG) (n = 38) and radical surgery group (n = 40). Patients in NRG received 2000~3000 cGy192 Ir irradiation of preoperatively intracavitary brachytherapy (radioactive source at 1 cm distance). After a rest of 10-14 days, radical hysterectomy with pelvic lymphadenectomy was performed. Surgery alone group (SAG) (n = 40) underwent radical surgery directly. The treatment outcomes between these two groups were compared, and the effect of preoperative intracavitary brachytherapy and presence of postoperative complications were evaluated.RESULTS:The total clinical efficacy for intracavitary brachytherapy was 94.7% (36/38) with complete response 13 (34.2%), partial response 23 (60.5%), and stable disease 2 (5.3%). Moreover, no patients developed progression disease; for SAG patients, 32 cases successfully finished the extensive hysterectomy and pelvic lymphadenectomy. Operation time <240 min was found in 19 patients. Moreover, the positive complication for lymphatic cyst and urinary retention was 20.0% and 15.0%, respectively. For NAG group, 36 patients successfully finished the extensive hysterectomy and pelvic lymphadenectomy. Operation time <240 min was found in 22 patients. Moreover, the positive complication for lymphatic cyst and urinary retention was 15.8% and 13.2%, respectively. The median follow-up time for NRG and SAG was 28 and 30 months, respectively. Three cases lost to follow-up in the SAG with the follow-up rate of 92.5% (37/40). In the NRG group, 3 cases lost to follow-up with the follow-up rate of 92.1% (35/38). The locoregional control rate for 1, 3, and 5 years was 80.0%, 61.3%, and 52.6%, respectively, for SAG group and 89.5%, 82.9%, and 76.9%, respectively, for NRG group with significant statistical difference for 3 and 5 years.CONCLUSION:Preoperative intracavitary brachytherapy is an effective procedure for the treatment for Stage Ib2 and IIa cervical cancer and can significantly improve the locoregional control rate.

Purpose To define the role of immediate-early 5 (IER5) gene as a promising biomarker in predicting the radiosensitivity and prognosis of cervical cancer patients receiving cisplatin-based concurrent chemoradiotherapy (DDP-CCRT). Results Our investigations found that IER5 level was markedly elevated in cervical cancer patients after being treated with irradiation, which indicated IER5 was closely dose induced. By contrast, the correlation between IER5 and radiosensitivity cannot be confirmed by the present study. The up-regulation of IER5 expression effectively increased cell apoptosis after administration of irradiation (P < 0.05). Using an ANOVA model for repeated-measures, we found significant association between the IER5 level and tumor size (P < 0.05). Materials and Methods Forty-three cervical cancer patients stage IIb-IIIb received DDP-CCRT were registered. Biopsy tissues were obtained after administration of irradiation dose of 0 Gy, 2~6 Gy, 10 Gy, 20 Gy, 30 Gy, respectively. The IER5 protein and mRNA levels were measured by immunohistochemistry, western blot and quantitative polymerase chain reaction, respectively; besides, the apoptosis rate was assessed by transferase-mediated dUTP nick end labeling. Conclusions Mechanistically, we confirmed that IER5 induced by radiation dose enhanced apoptosis of cervical cancer, was inversely associated with tumor size. In conclusion, our studies indicate target IER5 is improved to be a potential radiosensitizer for developing effective therapeutic strategies against cervical cancer to radiotherapy and a predictive biomarker for radiosensitivity.

目的:探讨动脉介入新辅助化疗对宫颈癌细胞增殖及凋亡的影响,为中晚期宫颈癌的临床治疗提供依据.方法:选择2012年8月-2015年5月在我院确诊为中晚期宫颈癌并行动脉介入化疗的患者60例作为研究对象.分别在入院时和动脉介入化疗后收集患者肿瘤组织标本,应用免疫组化法检测肿瘤细胞增殖指数(U),TUNEL法检测肿瘤细胞凋亡指数(AI).结果:经动脉介入化疗后,宫颈癌细胞的增殖指数显著降低,而凋亡指数显著提高,与化疗前比较,差异具有统计学意义(P＜0.05).宫颈癌细胞增殖指数LI随临床分期的增加而升高,而凋亡指数AI随临床分期的增加而降低,差异具有统计学意义(P＜0.05);介入化疗后,同一临床分期宫颈癌细胞的增殖指数LI均低于化疗前,而凋亡指数AI则高于化疗前,差异具有统计学意义(P＜0.05).结论:动脉介入新辅助化疗能够抑制中晚期宫颈癌细胞的增殖,并促进其凋亡,为患者创造手术切除的机会.

Objective To investigate the prognosis of high risk human papilloma virus (HPV) infection in patients with moderate cervical intraepithelial neoplasia (CIN) after LEEP treatment. Method 206 cases of CINII with positive high-risk HPV were treated with LEEP, and were tested for TCT, HCII and 23 HPV typing in 3, 6, 12, and 24 months dur-ing follow-up. Result In 206 cases of CINII patients, the rates of negative HR-HPV were 39.3%, 74.8%, 92.7%, and 96.6%in 3, 6, 12, and 24 months after treatment of LEEP, In those patients with positive HPV in 24 months during fol-low-up, 96.6%(199/206) patients finally had negative results, while in those who still had positive HPV, HPV load de-crease≥50%was seen in 1.9%(4/206), and there were 0.1%(2/206) and 0.4%(1/206) patients with HPV load decrease<50%and even increased HPV load. In 3 months after treatment, 54.0%(53/98) patients with HPV type 16 or multiple in-fection (type 16 included) had negative results, with significantly difference observed as compared with patients with oth-er high risk infection at 25.9%(28/108) (χ2=4.25, P<0.05);While in 6, 12 and 24 months after treatment, the HPV testing results were similar between those patients. Conclusion Most patients with cervical intraepithelial neoplasia grade 2 and high risk human papilloma virus infection will have negative results in 2 years after treatment, but infection of HPV type 16 or multiple infection that includes type 16 are similar with other high risk infection in respect of testing results in 6 months after treatment.

[Objective]To explore the application value of cisplatin chemotherapy combined with radiotherapy in the treatment of cervical cancer,and to analyze the relationship between clinical efficacy and expression levels of ribonucleotide reductase M1 (RRM1) in patients with cervical cancer.[Methods]Ninety-eight patients with cervical cancer were divided into group A and group B by the random number table method,49 cases in each group.Group B was given pelvic external radiotherapy combined with brachytherapy.On that basis,group A was given cisplatin chemotherapy.The short-term effects and incidence of side effects were compared between the two groups,and changes of the maximum transverse diameter of tumor and expression of RRM1 in cervical lesions before and after the treatment were recorded.According to short-term effects,Group A was further divided into the good to excellent and poor to medium groups.The expression of RRM1 in cervical lesions was compared between the two groups,and Pearson correlation analysis was used to analyze the relationship between the clinical effect and expression of RRM1 in cervical lesions.[Results]The overall effective rate,incidence rates of Ⅲ ～ Ⅳ level gastrointestinal reactions,Ⅰ ～ Ⅱ level bone marrow suppression,radiocystitis,and factitial proctitis in group A were significantly higher than those in group B (P ＜0.05).After treatment,the maximum transverse diameter of tumor and RRM1 high expression rates were significantly lower than those before treatment,and those in group A were lower than group B (P ＜0.05).The RRM1 low expression rate in excellent and good group was significantly higher than that in the medium and poor group (57.7％ vs 16.7％) (P ＜0.05).Pearson correlation analysis showed that the clinical effect of cisplatin chemotherapy combined with radiotherapy was positively correlated with RRM1 low expression in cervical lesions (P ＜0.05).[Conclusion]Cisplatin chemotherapy combined with radiotherapy improves the short-term effects of patients with cervical cancer,and its efficacy is inversely correlated with RRM1 expression.

Aim: To investigate expression pattern, clinical significance and potential roles of fyn related kinase (FRK) in cervical cancer.Methods: Expression of FRK protein and mRNA in 100 pairs of cervical cancer and matched non-cancerous tissue samples were detected by Western blot, immunohistochemistry and quantitative PCR, respectively. Statistical analyses were performed to evaluate associations of FRK protein expression with various clinicopathologic features and patients' prognosis. The effects of FRK on cell migration and invasion were examined using in vitro migration and invasion assays, respectively.Results: Weak/negative immunostaining of FRK protein was observed in 86 (86.00%) of 100 cervical cancer tissues. Low FRK expression was significantly associated with several aggressive clinicopathologic features of cervical cancer, such as higher International Federation of Gynecology and Obstetric stage (P = 0.01), the presence of lymph node metastasis (P = 0.01) and recurrence (P = 0.02). In addition, the survival analysis showed that cervical cancer patients with low FRK expression often had shorter overall survival than those with high FRK expression. The multivariate analysis also identified FRK expression as an independent prognostic factor of cervical cancer. Functionally, the enforced expression of FRK could efficiently inhibit cell migration and invasion of cervical cancer cells, but the knockdown of FRK dramatically enhanced cell migration and invasion.Conclusion: Our findings suggest that loss of FRK protein may be implicated into the tumorigenesis and cell motility of human cervical cancer. More importantly, FRK expression may function as a promising prognostic marker of this malignancy, highlighting its potentials as a candidate target for gene therapy. (C) 2016 Elsevier Masson SAS. All rights reserved.

Introduction: The aim of the retrospective study was to investigate the therapeutic efficiency of the preoperative intracavitary radiotherapy combined with radical surgery on postoperative complications and long-term survival in patients with stage IB2 and IIA2 cervical cancer (CC).Methods: From January 1995 to December 2012, a total of 171 patients with stage IB2 or IIA2 CC were recruited into the study. They were divided into two groups according to the treatment modality provided: preoperative radiotherapy followed by radical surgery (n = 80), and radical surgery alone (n = 91). The clinical curative effect, postoperative complications and the postoperative prognosis of patients were evaluated and compared in two groups. The tumor response and survival of patients in two groups were observed in follow-up study.Results: There were no significant differences in the incidence of postoperative complications, intraoperative blood loss and surgery duration (P > 0.05) between the two groups. Preoperative radiotherapy did not improve the postoperative prognosis yet. Though patients undergoing preoperative radiotherapy showed the similar 1-(92.50% vs. 84.62%), 3-(85.00% vs. 81.32%) and 5-year (80.00% vs. 74.72%) survival rates, the 3- and 5-year locoregional control rates of them were much higher than those undergoing surgery alone (P < 0.05).Conclusion: Preoperative radiotherapy combined with radical surgery could improve locoregional control rate and would not increase the risk of postoperative complications. It may be a feasible treatment mode for early stage CC carcinoma. (c) 2016 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.