目的 通过抗惊厥、镇静与烫伤治疗实验,研究合成的药用硫化汞纳米粉的主要药效,旨在探讨药用硫化汞纳米化对其药效学的影响.方法 (1)烫伤作用:取小鼠30只,随机数字表法分成3组,90℃水造成浅二度烫伤模型,模型组仅涂抹麻油,阳性对照组涂抹京万红,实验组涂抹硫化汞纳米粉膏剂,观察各组小鼠烫伤部位的结痂情况.(2)镇静作用:取小鼠50只,随机数字表法分为5组,模型对照组灌胃给予蒸馏水,阳性对照组腹腔注射地西泮片,实验组给予药用硫化汞纳米粉,连续给药7天,并于末次给药后,依次给各组小鼠腹腔注射戊巴比妥钠,记录睡眠潜伏期、睡眠发生率及睡眠时间.(3)抗惊厥作用:取小鼠50只,随机数字表法分为5组,模型对照组灌胃给予蒸馏水,阳性对照组腹腔注射给予地西泮片,实验组为药用硫化汞纳米粉.连续给药七天,并于末次给药后,依次给各组小鼠注射戊四唑,制造惊厥模型.观察小鼠惊厥发生率.结果 (1)烫伤作用:药用硫化汞纳米粉对伤口具有显著收敛作用(P≤0.01)(2)镇静作用:药用硫化汞纳米粉高、中、低剂量组均能显著减低睡眠潜伏期(P≤0.01)(3)抗惊厥作用:药用硫化汞纳米粉高、中、低剂量组惊厥率和死亡率明显较低(P≤0.01).结论 合成的药用硫化汞纳米粉具有治疗烫伤、镇静与抗惊厥的药理作用.

Conducted a literature review of the Mongolian Medicine Calcitum ashing agent modern research on the pharmacological action,chemical composition,and quality control.For the further study of the Mongolian Medicine,promotion,development and application to provide reference.

目的 探讨不同砷化合物致人表皮癌细胞(A431)的细胞毒性及氧化应激和砷代谢的情况.方法 培养的A431细胞分别暴露于0.05～50.0 μmol/L一甲基亚胂酸(MMAⅢ),0.05～200.0 μmol/L三氧化二砷(As2O3,As3+),0.5～500.0μmol/L砷酸氢二纳(As5+)和二甲基胂酸钠(DMAV)24 h,应用四甲基偶氮唑盐(MTT)法测定细胞生存率;检测砷化物对丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力的影响;以流式细胞仪检测细胞内活性氧(ROS);以原子荧光分析方法检测细胞内外不同形态砷含量.结果 在一定剂量范围的MMAⅢ(≥5.0 μmol/L)、As2O3(≥200.0 μmol/L)、砷酸氢二钠(0.5,≥200.0 μmol/L)和二甲基胂酸钠(500.0μmol/L)能够显著降低A431的细胞生存率(P＜0.05,P＜0.01),而在低剂量时,四种砷化物能显著促进A431细胞增殖(P＜0.05,P＜0.01).与对照组相比,50.0μmol/L MMAⅢ组,50.0、250.0 μmol/LAs2O3组,0.5 μmol/L砷酸氢二钠组MDA含量均显著升高(P＜0.05,P＜0.01),而250.0 μmol/L砷酸氢二钠和5.0～250.0μmollL二甲基胂酸钠组MDA含量均显著降低(P＜0.05,P＜0.01).与对照组相比,0.5、50.0 μmol/L MMAⅢ组,5.0～250.0μmol/L砷酸氢二钠组和0.5～500.0 μmol/L二甲基胂酸钠组的SOD活力显著升高(P＜0.05,P＜0.01).细胞内活性氧含量依次为0.5 μmol/L MMAⅢ＞50.0 μmol/L As2O3＞100.0 μmol/L砷酸氢二钠＞100.0 μmol/L二甲基胂酸钠.低剂量As2O3、砷酸氢二钠组细胞内砷甲基化率高于高剂量组,且As2O3组甲基化率高于砷酸氢二钠.结论 在本实验条件下,不同砷化物在低剂量促进A431细胞增殖,高剂量抑制增殖,可能与A431细胞的氧化应激和砷代谢有关.

OBJECTIVEHerein, the synthesis, component, microstructure and pharmacological and toxicology researches of the Synthetic Mercury Sulfide (S-HgS) a kind of common drug in Chinese, Mongolia, Tibetan medicine, and Indian medicine system were summarized. The similar cognition about mercury toxicity & pharmacological action from some Asian regions was analyzed, and it can supply some useful direction for the traditional Asian medicine system.METHODRecent literatures both domestic and abroad were summarized and analyzed.RESULTS-HgS is the basis of Vermilion, Mongolia-Vermilion, Zuotai, and Ras-sindoor. Athough the processes of synthesis are very different, but the microstructure and pharmacological & toxicology of S-HgS is similar.CONCLUSIONS-HgS has a far-ranging application,and unique curative effect. New technology such as nanotechnology can be used for improving the advancement of traditional Asian medicine.