Zisu（Perilla frutescens） is a medicinal and food homologous plant. It mainly contains volatile oils, flavonoids, triterpenoids, fatty acids, phenolic acids, anthocyanins and other chemical components. The types of Zisu（Perilla frutescens） compounds are complex, and it is not comprehensive to evaluate the quality by only a few index components. In this paper, based on the understanding of the new concept of quality marker（Q-marker）,combined with the current status of quality control of Zisu（Perilla frutescens）,the quality markers of Zisu（Perilla frutescens） are predicted and analyzed from 7 aspects, such as plant genetic relationship, correlation between components and drug efficacy, different processing methods, different producing areas and harvesting period, component measurability, traditional medicinal properties and different medicinal parts. The chemical components of Perilla aldehyde, Perilla ketone, Perilla isoone, Perilla ene, apigenin, luteolin and anthocyanin are used as the quality markers of Zisu（Perilla frutescens）,in order to provide reference for the quality control research of Zisu（Perilla frutescens）.

Objective:The anti-inflammatory mechanism of the leaves of Litchi chinensis Sonn.was studied by network pharmacological analysis and verified by in vitro cell experiments.Methods:The chemical constituents of the leaves of Litchi chinensis Sonn.were searched by literature.The active ingredients of the leaves of Litchi chinensis Sonn.were screened out by a traditional Chinese medicine systems pharmacology database and analysis platform（TCMSP） and the five principles of SwissADME.Each component target was predicted based on Pubchem database and Swiss target prediction database; inflammation genes were obtained from Disgenet database.The intersection target of the component target and inflammatory gene was obtained by Venny2.1.0 database as a potential anti-inflammatory target, and the protein-protein interaction（PPI） network diagram was constructed by inputting the target into STRING 11.0 database.Gene ontology（GO） enrichment analysis and Kyoto encyclopedia of genes and genomes（KEGG） pathway enrichment analysis of anti-inflammatory target genes were conducted by using DAVID 6.8 database.Cytoscape 3.8.0 software was used to construct the network diagram of active ingredients-anti-inflammatory targets-pathways of the leaves of Litchi chinensis Sonn.The effect of alcohol extract of the leaves of Litchi chinensis Sonn.on the activity of RAW264.7 cells was detected by CCK-8 method.The contents of tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6） were detected by enzyme-linked immunosorbent assay（ELISA）; the protein expression of cyclooxygenase-2（COX-2） was detected by Western blot.Results:Nine active ingredients were screened from the leaves of Litchi chinensis Sonn.,including 47 potential anti-inflammatory targets, 127 GO biological functions, and 68 KEGG signal pathways, involving TNF signal pathway, hypoxia-inducible factor 1（HIF-1） signal pathway, and other inflammation-related pathways.The cell experiments showed that the alcohol extract concentrations of the leaves of Litchi chinensis Sonn.of 12.5,25,and 50 μg/mL displayed no significant cytotoxicity in mouse peritoneal macrophage（RAW264.7）.The concentration could reduce the release of TNF-α and IL-6 generated by RAW264.7 cells induced by lipopolysaccharide and inhibit the expression of COX-2 protein.Conclusion:The constructed network diagram of components-targets-pathways reveals that the leaves of Litchi chinensis Sonn.exert anti-inflammatory effects through joint efforts of multi-components, multi-targets, and multi-pathways.The results can provide a theoretical basis for further elucidating the anti-inflammatory mechanism of the leaves of Litchi chinensis Sonn.

Jingjie（Schizonepeta tenuifolia Briq.） contains volatile oils, flavonoids and triterpenoids. It has the functions of antiviral, anti-inflammatory, analgesic and anti-tumor. This article combined the current status of quality control of SJingjie（Schizonepeta tenuifolia Briq.）. Based on the understanding of quality markers, the components which can be used as Q-markers of Jingjie（Schizonepeta tenuifolia Briq.） were analyzed from plant phylogeny and chemical composition specificity, different compatibility environments, traditional efficacy, traditional medicinal properties, measurable chemical composition, new medicinal effects and uses, different producing areas and different harvesting periods. The literature review found that the Q-markers of Jingjie（Schizonepeta tenuifolia Briq.） may be contained in volatile oils, terpenoids and flavonoids. Pulegone, limonene, caryophyllene, quercetin and menthone can be used as the candidate compounds for the selection of Q-markers of Jingjie（Schizonepeta tenuifolia Briq.）. It is expected to provide a reference for the quality control research of Jingjie（Schizonepeta tenuifolia Briq.）.

目的 研究分析华佗豆的化学成分,为后续的开发和利用奠定基础.方法 采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用法(UPLC-Q-Orbitrap HRMS),以正、负离子模式扫描采集数据,色谱条件为Thermo Gold C18色谱柱(2.1 mm×100 mm,1.9 μm),以0.1％甲酸乙腈(A)-1％甲酸水(含10 mmol乙酸铵)(B)为流动相,流量为0.3 mL/min,进行梯度洗脱.通过分析质谱数据,参考相关文献以及谱库比对,对华佗豆的化学成分进行鉴定.结果 共鉴定出华佗豆中的15个化合物,其中小檗碱、烟酰胺、咖啡因、柠檬酸、甘露醇、棉籽糖、乳果糖、羽扇豆醇、6,7-dehydro-11-hydroxy-12,13-epoxyterpendole A为首次从该植物中推测鉴定出来.结论 建立的方法快速分析鉴别了华佗豆的化学成分,为华佗豆的质量控制提供科学依据.

目的:研究龙眼叶乙酸乙酯提取物(简称龙眼叶提取物)对2型糖尿病大鼠降血糖作用机制.方法:采用高糖高脂饲料结合腹腔注射链脲佐菌素(STZ)制备2型糖尿病大鼠模型,以二甲双胍为阳性对照,考察龙眼叶提取物对2型糖尿病大鼠体质量、空腹血糖(FBG)、糖耐量(OGTT)、胸腺指数、胰岛素浓度(FINS)、超氧化物歧化酶(SOD)、丙二醛(MDA)、总胆同醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆同醇(LDL-C)、肿瘤坏死因子-α(TNF-oα)、白细胞介素6(IL-6)及胰腺病理形态的影响.结果:龙眼叶提取物能显著降低糖尿病大鼠的血糖,增强其葡萄糖耐受能力,并能提高糖尿病大鼠的IRI,同时对糖尿病大鼠有升高SOD活性、降低MDA含量的作用,抑制其胸腺萎缩.并能调节糖尿病大鼠的血脂紊乱情况,降低大鼠血清中TNF-α和IL-6的分泌.对糖尿病大鼠的胰腺病理形态特征也有一定的改善.结论:龙眼叶提取物对2型糖尿病大鼠有降血糖作用,其作用机制可能与修复胰岛细胞损伤、降低炎性反应因子水平及抗脂质过氧化过程有关.

目的:研究龙眼叶不同提取物对α-葡萄糖苷酶的抑制活性.方法:通过建立体外旷葡萄糖苷酶抑制模型,对龙眼叶提取物进行活性筛选,并对提取物浓度与抑制活性关系进行研究.结果:龙眼叶乙酸乙酯提取物(IC50=0.105 2 mg/mL)和正丁醇提取物(IC50=0.1261 mg/mL)均有较好的旷葡萄糖苷酶抑制作用,抑制活性大于阳性对照药阿卡波糖(IC50=0.1960mg/mL),且抑制活性呈浓度依赖性.结论:龙眼叶乙酸乙酯和正丁醇提取物对α-葡萄糖苷酶活性有抑制作用,具有开发治疗糖尿病降血糖药物的价值.

OBJECTIVE:To optimize the hypoglycemic active parts from the leaves of Dimocarpus longan for type 2 diabetes mellitus mice,and to provide reference for material basis study of it. METHODS:Total extract was obtained from the leaves of D. longan with 95% ethanol percolation extraction. After confirming hypoglycemic effect of total extract,extract was suspended with water. Relevant extract was obtained by using petroleum ether,ethyl acetate and n-butanol in turn(respectively marked as ST,YT,ZT and WT). The model of type 2 diabetes mellitus mice was established by high glucose and high fat diet combined with STZ. Model mice were randomly divided into model group(distilled water),positive control group(metformin,0.1 g/kg), ST low-dose,medium-dose and high-dose groups(22.2,29.6,44 g/kg,calculated by crude drug,similarly hereinafter),YT low-dose,medium-dose and high-dose groups(10.7,16,32 g/kg),ZT low-dose,medium-dose and high-dose groups(10.5, 15.8,31.6 g/kg),WT low-dose,medium-dose and high-dose groups(18.5,24.6,37 g/kg),with 10 mice in each group. Other 10 normal mice were included in normal group(distilled water). Those groups were given relevant medicine intragastrically,once a day,for consecutive 28 d.The growth of mice and the change of body weight were observed and recorded. Fasting blood glucose(FBG)of mice was detected,and glucose tolerance test of mice was conducted. The contents of TC,TG,HDL-C and LDL-C in serum of mice were also determined. RESULTS:Compared with normal group,body weight and serum content of HDL-C in model group were decreased;FBG and the blood sugar after giving glucose for 0.5,1,2 h and area under glucose tolerance curve of mice were increased,and serum contents of TC,TG and LDL-C were alse increased,with statistical significance(P<0.01). compared with model group,body weight of mice in YT high-dose group after administration of 25 d was increased,FBG of mice in YT groups and ZT medium-dose,high-dose groups were decreased(P<0.05 or P<0.01);blood sugar of mice in YT medium-dose,high-dose groups and ZT high-dose group after giving glucose for 1 h as well as blood sugar of mice in YT high-dose group after giving glucose for 2 h were all decreased;area under glucose tolerance curve of mice and serum content of TC were decreased in YT groups and ZT high-dose group,while serum content of HDL-C was increased. Serum content of TG and LDL-C in YT high-dose group and ZT high-dose group as well as serum content of TG in YT medium-dose group were all decreased,serum content of HDL-C in ZT medium-dose group was increased,with statistical significance(P<0.05 or P<0.01). CONCLUSIONS:The ethyl acetate part and n-butanol part from the leaves of D. longan show good hypoglycemic effect on type 2 diabetes mellitus mice.

Objective:To establish the quantitative method of linalool in Cortex Illicii.Methods:The PEG resilien-ce quartz capillary column(column length 30 m,ID 0.53 mm,film thickness 1μm),was employed,with temperature programming,the temperature was set at 230℃ both at injection port and detector.Results:The linear correlation of Iinalool was well in the range of 0.02μg～0.25μg(r=0.9994);The average recoveries of linalool were100.5%,with RSD of 2.2%.Conclusion: It was proved that the method was reliable and simple,it could be used as the determination method of the linalool in Cortex Illicii.

Objective To study the antitumor active fraction of roots of Actinidia deliciosa C.F.Liang et A.R.Ferguson in Guangxi.Methods The chemical constituents of roots of Actinidia deliciosa were extracted and isolated by systematic solvents.The 70% alcoholic extracts were divided into four portions including petroleum ether extract,ethyl acetate extract,n-Butyl alcohol extract and 95% alcoholic extract.Antitumor experiment of different extracts in vitro was done by MTT assay on human uterine carcinoma Hela,human gastric adenocarcinoma SGC7901,B16 melanoma cell,human hepatoma Hele7404,mouse entity tumor S180 and hepatoma H22.Results The growth of all tumor cells were inhibited by 70% alcoholic extract,ethyl acetate extract and n-Butyl alcohol extract from roots of Actinidia deliciosa.Conclusion 70% alcoholic extract,ethyl acetate extract and n-Butyl alcohol extracts have some antitumor effects in vitro.