Atherosclerosis (AS) is chronic inflammation in response to lipid accumulation. MicroRNA-155 (miR-155) is being increasingly studied to evaluate its potential as diagnostic biomarkers and therapeutic targets in many diseases. However, delineating the role of miR-155 in AS remains difficult. Here, we detected constitutive expression of several microRNAs (miRNAs) possibly associated with cardiovascular disease in foam cells and clinical specimens from patients with AS. Among them, we found that the level of miR-155 in foam cells was the most significantly elevated in a dose- and time-dependent manner. In addition, the expression of miR-155 was elevated in the plasma and plaque of patients with AS. We also reported for the first time that miR-155 targets calcium-regulated heat stable protein 1 ( CARHSP1 ), which regulates the stability of tumor necrosis factor alpha (TNF-α) mRNA. Furthermore, we investigated the mechanism by which the miR-155 level is elevated. miR-155 upregulation is due to transcriptional regulation by nuclear factor (NF)-κB, which is activated by the inflammatory factor TNF-α. In summary, increased miR-155 relieves chronic inflammation by a negative feedback loop and plays a protective role during atherosclerosis-associated foam cell formation by signaling through the miR-155–CARHSP1–TNF-α pathway.

目的:观察伽马刀治疗原发性肝癌的近期疗效及患者血清低氧诱导因子(HIF-1α)、血管内皮生长因子(VEGF)的动态变化.方法:选取在本院应用伽玛刀治疗的22例原发性肝癌患者(PHC)为研究对象,并将同期在我院体检的健康人群20例纳入对照组,观察PHC组放疗前后血清VEGF、HIF-1α水平的动态变化,以完全缓解、部分缓解视为有效,无变化、进展为无效,分析VEGF、HIF-1α水平与疗效的关系.结果:PHC组放疗前、放疗结束后1d、放疗结束后1个月的血清VEGF、HIF-1α水平显著高于对照组(P＜0.05);动态观察放疗期间PHC组上述血清学变化,放疗结束后1d血清VEGF、HIF-1α显著高于放疗前及放疗结束后1个月(P＜0.05).放疗后随访6个月,22例患者完全缓解(CR)1例,部分缓解(PR) 13例,无变化(NC)4例,进展(PD)4例.不同疗效患者放疗结束后1d血清VEGF、HIF-1α无明显差异,而放疗结束后1个月,NC+ PD患者血清VEGF、HIF-1α显著高于CR+PR患者(P＜0.05).结论:伽玛刀可有效控制肝癌进展,治疗期间PHC患者血清VEGF、HIF-1α水平常呈一过性升高,动态检测VEGF、HIF-1α可评估近期疗效.

目的：探讨联合检测超敏C反应蛋白（hs‐CRP）、降钙素原（PCT ）和B型脑钠肽（BNP）水平在慢性阻塞性肺疾病（COPD）急性恶化期的诊断价值。方法选择56例COPD稳定期患者及44例 COPD急性恶化组患者，分别测定其血常规、PCT、hs‐CRP及BNP ，同时进行痰细菌培养。结果 COPD急性恶化期患者的hs‐CRP、PCT、BNP水平明显高于COPD稳定期患者（P＜0．05），hs‐CRP、PCT、BNP的ROC曲线下面积分别为0．66、0．79、0．82，敏感性和特异性分别为80．2％、88．5％、91．3％和58．8％、71％、73．2％。联合检测hs‐CRP和BNP对COPD急性恶化期诊断的ROC曲线下面积为0．88，敏感性和特异性分别为92．8％和75．7％；联合检测PCT和BNP对COPD急性恶化期诊断的ROC曲线下面积为0．93，敏感性和特异性分别为96．3％和81．3％。结论在COPD急性恶化期，联合检测hs‐CRP、PCT 和BNP是较好的诊断指标，在呼吸系统细菌感染的判断上，PCT较hs‐CRP更有诊断价值，BNP可提示病情的严重程度。

To determine the role of miR-25 in non-small cell lung cancer (NSCLC), we first detected miR-25 expression in clinical specimens and lung cancer cell lines by quantitative real-time polymerase chain reaction. The levels of miR-25 were elevated in the plasma of NSCLC patients and NSCLC cell lines. Transfection of A549 and 95-D cells with a miR-25 inhibitor resulted in reduced cell proliferation and enhanced apoptosis. Moreover, the modulator of apoptosis 1 (MOAP1) gene was identified as a novel target of miR-25. The ability of miR-25 to promote cell proliferation and block apoptosis is attributable to its effect on MOAP1 suppression. In addition, miR-25 antagomir significantly inhibited lung cancer growth via upregulation of MOAP1 in a mouse xenograft model. Collectively, these data demonstrate that miR-25 is an important biomarker for lung cancer, and miR-25 promotes cell proliferation and inhibits apoptosis in NSCLC cells by negatively regulating MOAP1 expression.

BACKGROUND:Primary hepatocellular carcinoma (HCC) is one of the most common malignancies in the world. However, the molecular pathogenesis of HCC is not well-understood, and the prognosis for patients with HCC remains very poor.METHODS:To disclose detailed genetic mechanisms in hepatocellular carcinoma (HCC) with a view toward development of novel therapeutic targets, we analyzed expression profiles HCCs and their corresponding noncancerous tissues by using bioinformatics method.RESULTS:In this paper, we report the identification of genes whose expression has been altered and the changed bio-pathways during hepatocarcinogenesis. Hepatoma cells infect intracellular and intercellular signal transduction through Focal adhesion and cause abnormal expression of important intracellular signaling pathway. In addition, it is worth mentioning that some small molecules still restored to the state similar to normal cells, such as bambuterol and lovastatin. This member gene set would serve as a pool of lead gene targets for the identification and development of novel diagnostic and therapeutic biomarkers to greatly improve the clinical management of HCC patients with different risks of recurrence after curative partial hepatectomy.CONCLUSIONS:The study has great significance for gene therapy and pharmacotherapy and provides a new treatment entry point and a potential new clinical drug for HCC patients.

BackgroundExpression patterns of microRNAs in serum are involved in potentially non-invasive biomarkers for various diseases. The purpose of this study is to examine the expression of miR-21 in serum of patients with diffuse large B-cell lymphoma (DLBCL) and to validate the significance of miR-21 in early diagnosis, genotyping, treatment options as well as its prognosis estimates of Chinese DLBCL.MethodsmiR-21 expression was detected by fluorescent quantity polymerase chain reaction (qPCR) in 9 DLBCL cell lines (OCI-Ly1, OCI-Ly3, OCI-Ly4, OCI-Ly7, OCI-Ly8, OCI-Ly10, OCI-Ly18, OCI-Ly19, and HBL), as well as in tumor tissue and serum samples from patients with DLBCL (germinal center B-cell-like (GCB) DLBCL 32; activated B-cell-like (ABC) DLBCL 30) and 50 healthy subjects.ResultsExpression of miR-21 was increased in DLBCL cell lines. Compared with the miR-21 expression of GCB subgroup (OCI-Ly1, OCI-Ly4, OCI-Ly7, OCI-Ly8, OCI-Ly18, OCI-Ly19), ABC subgroup (OCI-Ly3, OCI-Ly10, and HBL) has higher expression (t=11.18, P<0.01). Circulating miR-21 level in sera from patients with DLBCLwas associated with matched tumor tissue (r(2)=0.931, P<0.0001). Consistent with the in vitro, miR-21 expression levels in serum of patients with DLBCL [21.38(10.26-45.21)] were higher than those in serum of control cases [1.87(1.05-3.97); U=168, P=0.000]. Moreover, miR-21 expression levels in serum of patients with subgroup ABC [28.68(14.92~98.44)] were higher than that of patients with subgroup GCB [18.3(7.32~33.46); U=336, P=0.043]. miR-21 expression in serum of DLBCL with stage I and II were higher than those in stage III and IV (U=62, P=0.013 in GCB type; U=53, P=0.014 in ABC type). Compared with relapse-free survival in patients with DLBCL, high expression of miR-21 was associated with well prognosis (U=259, P=0.035).ConclusionmiR-21 expressed in the serum of patients with DLBCL from Chinese was associated with clinical stage, molecular subgroup, and prognosis estimates. miR-21 may be served as a biomarker in early diagnosis, genotyping, treatment options, and prognosis estimating of Chinese DLBCL.

Objective To investigate the expression levels of serum visfatin ,leptin and other indexes and their correlation and clinical significance in the patients with type 2 diabetes mellitus(T2DM ) .Methods Eighty-two age-matched and gender-matched cases of T2DM (T2DM group)and 71 cases of healthy subjects(health control group)were tested serum visfatin ,leptin ,insulin ,lipid and glycemia levels .Results The serum visfatin ,leptin ,triglycerides(TG) ,total cholesterol(TC) ,high-density lipoprotein choles-terol(HDL-C) and low density lipoprotein cholesterol(LDC-C) ,fasting plasma glucose ,fasting insulin(FINS) ,homeostasis model assessment of insulin resistance(OMA-IR) ,2 h FPG ,2 h FINS had statistically significant differences between the T 2DM group and the health control group(P< 0 .05);the stepwise regression analysis showed that visfatin were positively correlated with BMI , FINS ,HOMA-IR ,TC ,TG ,LDL-C (P<0 .05)and negatively correlated with HDL-C(P<0 .05);leptin were positively correlated with BMI ,FINS ,HOMA-IR(P<0 .05) .Conclusion Serum visfatin and leptin in the T2DM patients are hither than those in the healthy subjects and closely related with blood lipid ,blood glucose and insulin ,which may become the new diagnostic indexes of T2DM .

Various studies have reported that Huaier possesses anti‐tumor effects. However, the mechanisms are not completely elucidated. Here, we found 66 differentially expressed miRNAs in Huaier‐treated pulmonary adenocarcinoma A549 cells, with upregulation of miR‐26b‐5p. Transfection of A549 cells with miR‐26b‐5p mimic inhibited proliferation and induced apoptosis, while transfection of Huaier‐treated A549 cells with a miR‐26b‐5p inhibitor reversed the effects of Huaier. EZH2 was verified as the target of miR‐26b‐5p. Thus, our findings indicate that Huaier might suppress proliferation and induce apoptosis in lung cancer cells via a miR‐26b‐5p‐EZH2‐mediated approach, which provides a new perspective for understanding the anti‐tumor effects of Huaier.

Objective To investigate the dysregulation of microRNA-500 (miR-500) in the development process from hepatitis C liver cirrhosis to hepatocellular carcinoma so as to clarify the role of change of miR-500 expression in the development process from the hepatitis C to liver cirrhosis and hepatocellular carcinoma.Methods From Nov.2012 to Oct.2013,8 cases of hepatitis C liver cirrhosis and 8 cases of hepatitis C virus infection-related liver cancer were selected as the research objects,and 8 cases of the healthy check-up served as the control group.The expression level of miR-500 in the plasma of the above three groups was tested by using real-time quantitative polymerase chain reaction (Real-time PCR).Results The expression level of miR-500 in liver cirrhosis group was 5.72 ± 2.28,higher than 0.95 ± 0.48 in the control group,and that in liver cancer group was 18.62 ± 8.13,higher than in the control group and liver cirrhosis group.Conclusion The miR-500 may be involved in the development process from chronic hepatitis C liver cirrhosis to liver cancer.

Objective:To reveal the relationship between procalcitonin(PCT)level and pathogens in agranulocytosis patients with fever after chemotherapy of blood disease.Method:A total of 242agranulocytosis patients with fever after chemotherapy of blood disease were collected in our hospital during Jul 2010to Sep 2012.Samples were divided into infectious fever group and FUO group according to characteristics of the etiology,imaging,and symptoms.And the infectious fever group included bacterial and non-bacterial infections subgroup.Then,compared the difference of PCT level among groups,and analyzed the effect of the PCT on blood culture result.Result:9.3%samples in FUO group were PCT positive(≥0.5ng/ml),while 69.2% samples in bacterial infections fever subgroup were PCT positive.34.5% samples in non-bacterial infections fever subgroup were PCT positive.For the blood culture results,PCT positive rate significantly increased(83.3%).And the sensitivity of PCT was 85.7%with negative predictive value of 95.7%.Conclusion:PCT could be used as a preliminary indicator of bacterial infections fever in the diagnosis of agranulocytosis with fever.And it also could be used to eliminate bacterial infections in blood,help to identify pathogens and infer the reasons of fever.Thus,this will be beneficial to guide the clinical medication treatments of agranulocytosis with fever after chemotherapy of blood disease.

Objective To study the expression of microRNA-21 ( miR-21 )in serum of patient with diffuse large B cell lymphoma (DLBCL) and DLBCL cell lines and validate the significance of miR-21 in early diagnosis,genotyping and prognosis estimates of DLBCL.Methods miR-21 expression were detected by fluorescent quantity polymerase chain reaction (FQ-PCR)in 9 lymphoma cell lines (OCI-Ly1,OCI-Ly3,OCI-Ly4,OCI-Ly7,OCI-Ly8,OCI-Ly10,OCI-Ly18,OCI-Ly19 and HBL),the serum from DLBCL patients (n =62) and health controls (n =50 ).Kaplan-Meier survival analysis was carried out during the relapsefree survival period of DLBCL patients to explore the relationship between the prognosis and microRNA expression level.Results Real time FQ-PCR result indicated that miR-21 expression was higher in DLBCL cell lines than that in normal B cells (BC).miR-21 expression in normal B cell and 9 DLBCL cell lines separately were 1.04 ± 0.02,2.30 ± 0.35,237.97 ± 56.19,5.27 ± 0.83,3.40 ± 0.30,11.22 ± 2.70,133.55 ± 16.78,6.63 ±0.24,4.91 ±0.37 and 81.59 ±6.64.Compared with BC,the expression of miR-21 were higher in all 9 DLBCL cell lines ( t =7.3,13.7,21.0,6.2,8.8,13.6,6.5,39.5,18.1 ;P ＜ 0.01 ).miR-21 expression segregates with specific molecular subgroups of DLBCL The expression was higher in the ABC type cell lines (OCI-Ly3,OCI-Ly10,HBL) than GCB type cell lines (OCI-Ly1,OCI-Ly4,OCI-Ly7,OCI-Ly8,OCI-Ly18,OCI-Ly19;t =11.18,P ＜ 0.01 ).Consistent with the cell line models,miR-21 expression levels were higher in serum from DLBCL patients [21.38 (10.26-45.21 )] than from controls [1.87 ( 1.05-3.97 ),U =168,P =0.000],and the levels were higher in DLBCL cases with an ABC-type [28.68 ( 14.92-98.44 )] than those in GCB-type [18.30 ( 7.32-33.46 ),U =336,P =0.043].MiR-21 expression levels were different in sera from different clinical stage DLBCL patients.The miR-21 level in serum of patients with subgroup ABC and subgroup GCB in stage Ⅰ and Ⅱ were 47.49( 25.65-295.41 ) and 24.74( 16.08-50.38) respectively and in stage Ⅲ and Ⅳ were 16.66 ( 5.35-44.30 ) and 11.96 ( 4.10-21.05) respectively.The levels were higher in DLBCL cases with Ⅰ -Ⅱ stage than those with Ⅲ-Ⅳ stage (U =62,P =0.013 in GCB type; U =53,P =0.014 in ABC type).Moreover,compare with relapse-free survival in DLBCL patients,high miR-21 expression was associated with well prognosis ( U =259,P =0.035).Conclusions MiR-21 is high expression in DLBCL cell lines and DLBCL patients serum.miR-21 level in sera from DLBCL patients is associated with clinical stage,molecular subgroup and prognosis estimates.MiR-21 may serve as a new biomarker to early detection,genotyping and prognosis estimates of DLBCL.

Objective To study the expression of serum circulating miR-21 in the patient with lymphoma and to validate its significance in early diagnosis of lymphoma.Methods We detected serum circulating miR-21 expression by FQ-PCR in lymphoma patients(n=60) and health controls(n=50).At the same time,we detected miR-21 expression by FQ-PCR in matched tumor tissue and serum from diffuse large B cell lymphoma Cymphoma patients(n=30).Results FQ-PCR results indicated miR-21 higher expression in serum from Cymphoma patients than controls(t=6.9,P<0.0001),and peripheral circulating miR-21 expression levels had very close correlation with those that matched tumor tissue in lymphoma cases(r2=0.931,P<0.0001).Conclusion Circulating miR-21 may be expected to be as a new biomarker to early detection of lymphoma.

目的:探讨引起成分献血者发生不良反应的因素,制订出相应的预防及处理措施,减少不良反应的发生,保证献血者的安全和血液成分产品的质量,促进无偿成分献血者的招募和保留.方法:分析献血不良反应发生的原因,实施不良反应的预防及处理措施,对无偿成分献血者的献血全过程精心护理.结果:献血不良反应的发生与性别、献血次数等因素密切相关,献血不良反应中低钙反应发生率最高.通过采取相应的预防及处理措施,使无偿成分献血者轻松、愉快的完成献血过程,成为固定的无偿成分献血者.结论:针对不同的原因采取相应的预防及处理措施,可大大减少献血不良反应的发生,保证献血者的安全和血液成分产品的质量,促进无偿成分献血者队伍的稳定、壮大.

Objective To study the expression of microRNA-301 in pancreatic carcinoma andvalidate the significance of miR-301 in invasion and metastasis of pancreatic carcinoma.Methods miR-301 expression were detected by FQ-PCR in 5 pancreatic cancer eell lines(PANC-1,PaCa-2,AsPC-1,Hs766T.BxPC-3).Further immunohistochemistry in pancreatic cancer tissue microarrays was detected miR-301 expression,which contained 60 pancreatic cancer specimens along with 10 normal adjacent tissues and 10 normal pancreas tissues.After high expression of miR-301 in pancreatic carcinoma being confirmed.the clinical significance of high expression of miR-301 in invasion and metastasis of pancreatic carcinoma were studed.Pancreatic cancer cell lines(PANC-1.PaCa-2)were transfected by 100 nmoml/L miR-301 inhibitor(anti-miR-301)or negative eontrol(Anti-miR~(TM) Negative Control#1).COX-2 and MMP-2 protein expression in pancreatic cancer cell lines were detected by WB.and cell migration assays were performed using transwell technology.Results FQ-PCR resuhs indicated that miR-301 expression was higher in pancreatic cancer cell lines than normal pancreatic cells.The relative level of miR-301 in 5 pancreatic cancer cell lines(PANC-1,PaCa-2,AsPC-1,Hs-766T,BxPC-3)and normal pancreatic cell were 33.09± 4.21,30.76±3.18,47.57±3.56,20.20 ±1.21,76.75±13.51 and 1.00±0.08 respectively.The miR-301 level in all 5 pancreatic cancer cells were significantly higher than those of normal pancreatic cell(t=8.86,9.53,6.39,6.77,11.18,P<0.01).Immunohistochemistry results also showed miR-301 expression was higher in pancreatic carcinoma tissues than those in the cancer adjacent tissues and normal pancreatic tissues.The relative levels of miR-301 in pancreatic carcinoma tissues.normal adjacent tissues and normal pancreas tissues were 0.88±0.09,0.22±0.04 and 0.14±0.05 respectively.The miR-301 levels in pancreatic carcinoma tissues were significantly higher than those of normal adjacent tissues and normal pancreatic tissues(t=15.1,10.6,P<0.01).There was no significant difference between normal adjacent tissues and normal pancreas tissues(t=1.32,P=0.22).After miR-301 inhibitor was introduced into pancreatic cancer cells PANC-1 and PaCa-2.miR-301 levels were reduced while the protein levels of COX-2 and MMP-2.which were invasion and metastasis related factors,were down-regulated.The cell migration assay indicated the numbers of PANC-1 and PaCa-2 cells,which migrated to lower chamber.were 587±27 and 363±13 respectively after miR-301 inhibitor was applied.The numbers of migrated cells were 1091 4-15.737±44 when the netative control was applied.The cell invasion ability was decreased significantly in the inhibitor group compared with the negative group(t=7.89,7.56,P<0.01).Conclusions miR-301 is highly expressed in pancreatic cancer cell lines and pancreatic cancer tissues.Inhibition of miR-301 expression can effectively supress the invasion of pancreatic cancer cells.miR-301 may serve as a new biomarker for early detection of pancreatic cancer and molecular target for early treatment of pancreatic cancer.

In order to monitor and control the unable managing locale,we design wireless monitoring system based on Samsung S3C2410.And,it' s controlled on wireless way by internet with GPRS/CDMA technology.

Objective:To investigate the clinical value of serum level of vascular endothelial growth factor(VEGF)in patients with lung carcinoma and to explore the mechanism of VEGF affecting lung carcinoma growth,development and prognosis.Methods:The serum level of VEGF was measured by enzyme-linked immunosorbent assay.The correlation between serum VEGF levels and blood cell counts was assessed by linear regression analysis.The relationship between the survival time and the serum level of VEGF was analyzed by the Kaplan-Meier method.Results:The serum level of VEGF was significantly higher in patients with lung cancer(1251.6±427.4 ng/L)than that in control group(343.8±171.3 ng/L).There was significant difference in serum level of VEGF between patients with different clinical stage.There was positive correlation between the serum level of VEGF and platelet counts(r=0.523,P0.01).Serum VEGF levels also correlated with leukocyte counts(r=0.511,P0.01).Survival analysis showed that the high serum VEGF was associated with a high risk of death.Conclusion:The serum VEGF may be an assisting diagnostic marker for lung cancer.VEGF plays an important role in lung carcinoma development.The serum level of VEGF may be a reliable predictor for prognosis.VEGF is secreted by tumor cells and subsequently released into blood.It may be the mechanism of VEGF to promote tumor growth and metastasis.