To explore the role of the miRNA-1297/phospholipase Cβ1 (PLCβ1) axis in intestinal barrier injury. Abnormally expressed miR-1297 and its target gene PLCβ1 as well as their transcriptome sequencing were confirmed by bioinformatics analysis. Next, the intestinal barrier injury was induced by lipopolysaccharide (LPS) in the CCCHIE-2 cells. Subsequently, the impacts of miR-1297 and PLCβ1 on the transcriptome were estimated. QRT-PCR and Western blotting were conducted to detect the relative mRNA and protein expressions, respectively. The cell viability and permeability were analyzed by MTT assay and fluorescent yellow detection. miR-1297 was significantly upregulated in patients with human immunodeficiency virus/acquired immunodeficiency syndrome and targeted PLCβ1. Moreover, overexpressed PLCβ1 was mainly enriched in the transforming growth factor-beta signaling pathway, while the knockdown of miR-1297 was focused on the arginine biosynthesis pathway. The overexpression of miR-1297 could reduce the PLCβ1 expression and inhibit the viability of CCCHIE-2 cells injured by LPS, while the effect of the downregulation of miR-1297 was on the opposite. Western blotting and cell fluorescence localization experiments revealed that the inhibition of miR-1297 increased the expressions of PLCβ1 and ZO-1. In addition, the upregulation of miR-1297 strengthened the permeability in cells injured by LPS, as did the knockdown of PLCβ1. miR-1297 could restrain the repair of intestinal barrier injury via negatively regulating PLCβ1 and its tight junction downstream protein ZO-1 in CCC-HIE-2 cells injured by LPS, which indicated that PLCβ1 and miR-1297 might be important targets for the repair of intestinal barrier injury.

Although accumulating evidence has revealed that metallothioneins (MTs) and its family member MT2A are strongly linked to the risk of various solid tumors, researches on the occurrence and development of acute myeloid leukemia (AML) have rarely been investigated. Here, we constructed a lentiviral vector with MT2A over-expression and the interfering plasmids with MT2A expression inhibition to study the influence of MT2A on the bioactivities of HL60 cells. After cells were infected with a lentiviral vector containing the MT2A gene, both transcription and translation levels of MT2A were significantly increased in the over-expressed group in comparison with control groups. In vitro experiments, all results demonstrated that cell reproductive capacity was inhibited, but cell apoptosis rate was significantly increased. Together, the expression of apoptosis-related protein Bcl2 was remarkably reduced, while a high expression level of Bax protein was detected. Further experiments revealed that up-regulation of MT2A induced cell apoptosis and promoted G2/M phase arrest. The mechanism may be associated with down-regulated p-IκB-α and cyclinD1 expression and up-regulated IκB-α expression in the nuclear factor-kappaB (NF-κB) pathway. On the contrary, MT2A expression was down-regulated by interfering plasmids. We found that cell proliferative potential was notably increased in the interfering group compared with the negative and untreated group. What's more, MT2A may be closely related to AML cell proliferation and function via the NF-κB signal pathway.

Epithelial ovarian cancer (EOC) contributes the majority of death cases among various ovarian malignancies. Although a standard method of treatment is the surgical removal of malignant tissue followed by platinum-based chemotherapy, a group of patients does not respond appropriately to cisplatin. An appropriate response to cisplatin has been linked with the nucleotide excision repair mechanism. The present study aims to investigate the role of polymorphisms in DNA repair genes, excision repair cross-complementation group 1 (ERCC1) with susceptibility to EOC development and tumour response to platinum-based chemotherapy in Chinese EOC patients. Patients (n = 559) reporting to the Department of Oncology and general surgery, the First Affiliated Hospital of Kunming Medical University, were enrolled in the study. Three hundred twenty-three healthy controls hailing from similar geographical areas without a history of cancer enrolled as healthy controls. Excision repair cross-complementation group 1 polymorphisms (rs11615, rs3212986, rs735482, rs2336219, rs3212980, rs3212964, rs3212961 and rs2298881) were genotyped by appropriate methods. Distribution of genotypes and allele for ERCC1 polymorphisms (rs11615, rs3212986, rs735482, rs2336219, rs3212980, rs3212964, rs3212961 and rs2298881) were comparable among healthy controls and EOC patients. Interestingly, homozygous mutant and the minor allele for rs11615 and rs3212986 polymorphisms were significantly higher in nonresponder EOC patients when compared to those with a proper response to cisplatin treatment. The prevalence of other SNPs was comparable among the two treated clinical categories. Furthermore, combined genotype revealed significant association of rs11615: TT/ rs3212986: AA genotype combination with cisplatin nonresponder. Variants of rs11615, rs3212986 polymorphisms are associated with cisplatin resistance in Chinese EOC patients. Combined rs11615 and rs3212986 genotypes can be used as a predictive biomarker for platinum-based chemotherapy outcomes.

Objectives: The rapid spread of Klebsiella spp. is recognised as a major threat to public health owing to a rise in the number both of healthcare- and community-acquired infections. Here we report the draft genome sequence of a high carbapenem-resistant Klebsiella quasipneumoniae subsp. quasipneumoniae strain (Cln185) isolated from a human immunodeficiency virus (HIV)-positive patient with pneumonia. Methods: Classical microbiological methods were applied to isolate and identify the strain. Genomic DNA was sequenced using an Illumina HiSeq platform and the reads were de novo assembled into contigs using CLC Genomics Workbench. The assembled contigs was annotated and whole-genome sequencing (WGS) was performed. Results: WGS analysis revealed that the genome comprised a circular chromosome of 5 406 774 bp with a GC content of 57.73%. Three important antimicrobial resistance genes (bla(IMP-38), bla(OKP-B-6) and bla(DHA-1)) were detected. In addition, genes conferring resistance to aminoglycosides, beta-lactams, fluoroquinolones and tetracycline were also identified. Conclusion: The draft genome sequence reported here will lay the foundation for future research on antimicrobial resistance and pathogenic mechanisms in K. quasipneumoniae subsp. quasipneumoniae and also will promote comparative analysis with genomic features among different sources of clinically important multidrug-resistant strains. (C) 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.

Objective To investigate the clinical efficacy and safety of recombinant activated factor Ⅶ(rFⅦa)in the treatment of spontaneous intracerebral hemorrhage.Methods One hundred and twenty-eight cases of spontaneous intracerebral hemorrhage treated in this hospital were collected as the study subjects and randomly divided into the control group(59 cases)and observation group(69 cases).The control group was given the routine liquid infusion therapy plus ethamsylate;while on this basis the observation group was added with rFⅦa therapy.The change of hematoma volume was evaluated by using CT plain scanning after 24 h treatment.The NIHSS score was used to evaluate the recovery situation of the two groups after 3,7 d treat-ment.The Rankin scale(mRS)and ADL scores were used to evaluate the long term prognosis on 30,90 d after treatment;the occurrence situation of adverse reactions in the two groups during the therapeutic process was recorded.Results After 24 h treatment,the enlarged volume of hematoma in the observation group was less than that in the control group,the difference between them was statistically significant(P<0.05);the symp-toms after 3,5 d treatment in the two groups were significantly improved,the NIHSS score in the observation group was significantly better than that in the control group,the difference between the two groups was statis-tically significant(P<0.05);after 90 d treatment,the mRS score and ADL score in the observation group were better than those in the control group,the differences were statistically significant(P<0.05);the adverse re-actions had no statistically significant difference between the two groups(P>0.558).Conclusion rFⅦa has significant effect for treating spontaneous cerebral hemorrhage,can effectively improve the recovery of neurological function and does not increase the incidence of adverse reactions,and is a safe and effective treatment method.

Objective To investigate the possibility and superiority of array-based comparative genomic hybridization (array-CGH) applied in clinical cytogenetic diagnosis.Methods Comparative analysis was performed between G-brnding karyotype analysis and array-CGH.Both G-banding karyotype analysis and array-CGH were performed on 10 cases referred to the Clinical Medical Research Center Shenzhen People's Hospital for cytogenetic analysis between April 2008 and April 2010,the results of both methods were compared.Fluorescence quantitative polymerase chain reaction (FQ-PCR) and fluorescence in situ hybridization (FISH) were used to verify the results of G-banding karyotype analysis and array-CGH.Results Compared with G-banding karyotype analysis,9 cases' karyotypes except one complex chromosomal rearrangement (CCR),were detected by array-CGH accurately:the source and property of the derivative chromosomes in case 1 and case 2 were accurately determined;CCR in case 3 was indicated to be balanced;the regions,sizes,and breaking points of aberrant chromosome in case 4 were precisely determined;the source and property of the chimeric marker chromosome in case 10 were accurately determined;a large number of submicroscopic copy number variations (CNVs) undetected by G-banding karyotype analysis were detected,including 6 potential pathological CNVs.The results of array-CGH were confirmed by FQ-PCR and FISH.Conclusion Compared with conventional cytogenetic technique,arrayCGH is of comprehensive,high resolution,sensitive,rapid,accurate,less sample demand,which can serve as a useful complement for G-banding to be used in the clinical cytogenetic diagnosis.

目的 :评价STA-Liatest纤维蛋白（原）降解产物[fibrin（ogen）degradation products,FDP]试剂在STA-R Evolution全自动血凝仪上的性能。方法 :选择质控血浆、患者血浆及健康成人血浆样本总共360份,在全国范围内按不同地域分布选取了6家医院进行多中心同步实验检测。分别使用STA-Liatest FDP和N-FDP,在STA-R Evolution全自动血凝仪上行FDP检测,检测结果参考美国临床实验室修正法规1988（CLIA88）标准要求进行统计分析。结果:STA-Liatest FDP多中心总的批内重复性,变异系数分别为8.0%（质控水平1）和4.7%（质控水平2）;与N-FDP的相关系数为0.718（P＜0.05,FDP＜5μg/m L）和0.983（P＜0.01,FDP≥5μg/m L）;稳定性验证中,同一瓶试剂连续使用3 d的重复性,变异系数≤11.5%。结论 :STA-Liatest FDP的上述性能指标符合临床实验室的质量要求,可为临床提供可靠的检测结果。同时,为保证检测系统的溯源性,建议实验室尽可能使用配套试剂,以最大限度保证检验结果的可靠性。

Activation-induced deaminase (AID), a cytidine deaminase, can accelerate the acquisition of BCR-ABL1 kinase domain mutations in human CML. In the present study, we investigated the expression of AID and Bcr-Abl in CML cells derived from 35 clinical patients. We found that both AID and Bcr-Abl were correlatively over-expressed in CML-LBC (lymphoid blast crisis) cells as compared with those in CML-CP (chronic phase) cells. AID expression was significantly decreased in CML-LBC cells after treated with arsenic trioxide, especially together with imatinib. We also observed satisfied therapy effects of As(2)O(3) and imatinib on patients with CML blast crisis. These data suggest that decreasing AID expression in CML-LBC by As(2)O(3) may be a promising approach to CML treatment.

目的:探讨幼儿浆细胞白血病(PPCL)的临床及形态学特点。方法:对1例幼儿患浆细胞白血病的临床及实验室检查进行回顾性研究。结果:①幼儿浆细胞白血病多以发热、出血、贫血、感染及全身多处淋巴结肿大为主要临床表现;②血常规:WBC:0.81×109/L,浆细胞占26%。骨髓检验,浆细胞系统明显增生,占有核细胞的70.5%;③生化检查:各项指标暂无明显异常,提示目前没有组织和器官的病变。各项免疫球蛋白数值没有明显升高;④血培养为铜绿假单胞菌感染;⑤免疫分型表现为浆细胞及B淋巴细胞。结论:幼儿患浆细胞白血病多为原发性,起病急,多以发热、出血、贫血、感染及全身多处淋巴结肿大为主要临床表现,免疫球蛋白不升高,其它组织器官的病变暂不明显,应注意与其他急性白血病相鉴别。

目的观察深静脉血栓形成（DVT）患者应用不同起始剂量华法林治疗时,在相应时间国际标准化比值（INR）的变化,比较INR的达标率及出血并发症的发生率,以期对临床应用华法林抗凝治疗提供实验室依据。方法选取具有华法林抗凝治疗适应证的DVT患者56例,分为A组（37例）华法林起始剂量为2.5 mg;B组（19例）华法林起始剂量为5 mg,于治疗第3、4、5、8天测定INR,根据INR值调整华法林剂量,直到INR达到预期值（INR在2.0～3.0之间）。结果与2.5mg起始剂量组相比,5 mg起始剂量组INR变化速度较快,第5、8天INR的达标率显著高于2.5 mg起始剂量组。本文无1例出现并发症者。结论以5 mg为起始剂量治疗DVT患者,能使INR迅速、安全且有效地达到预期值,而无出血并发症发生。

我们对我院新购置Coulter-750全自动血细胞分析仪在白血病诊断中的应用进行初步观察. 一、材料和方法 1.标本一般血液检查正常健康体检者30名,各型血液病患者45例,其中急性淋巴细胞白血病(ALL)10例,慢性淋巴细胞白血病(CLL)2例,急性粒细胞白血病未分化型(M1)1例,部分分化型(M2)13例,急性多颗粒早幼粒细胞白血病(M3)4例,急性粒-单细胞白血病(M4)9例,急性单核细胞白血病(M5)6例,每例取血2ml置于15%EDTA-K2 0.048 ml的真空抗凝管中混匀,取血后2 h完成测试.