ObjectiveHistone modification has a significant effect on gene expression. Enhancer of zeste homolog 2 (EZH2) contributes to the epigenetic silencing of target chromatin through its roles as a histone-lysine N-methyltransferase enzyme. The development of anoikis resistance in tumor cells is considered to be a critical step in the metastatic process of primary malignant tumors. The purpose of this study was to investigate the effect and mechanism of anoikis resistance in ovarian adenocarcinoma peritoneal metastasis.MethodsIn addition to examining EZH2 protein expression in ovarian cancer omental metastatic tissues, we established a model of ovarian cancer cell anoikis and a xenograft tumor model in nude mice. Anoikis resistance and ovarian cancer progression were tested after EZH2 and N6-methyladenosine (m6A) levels were modified.ResultsEZH2 expression was significantly higher in ovarian cancer omental metastatic tissues than in normal ovarian tissues. Reducing the level of EZH2 decreased the level of m6A and ovarian cancer cell anoikis resistance in vitro and inhibited ovarian cancer progression in vivo. M6a regulation altered the effect of EZH2 on anoikis resistance.ConclusionOur results indicate that EZH2 contributes to anoikis resistance and promotes ovarian adenocarcinoma abdominal metastasis by m6A modification. Our findings imply the potential of the clinical application of m6A and EZH2 for patients with ovarian cancer.

目的 探讨卵泡刺激素（FSH）/黄体生成素（LH）、同源框基因A11（HOXA11）、妊娠相关血浆蛋白A（PAPP-A）与子宫瘢痕妊娠（CSP）患者子宫动脉栓塞术后再妊娠结局关联性。方法 98例接受子宫动脉栓塞术治疗且有生育需求CSP患者，根据术后1年内是否成功再妊娠分为成功组（38例）、未成功组（60例），比较两组一般资料、术前、术后1个月、术后2个月FSH/LH、HOXA11、PAPP-A。结果 成功组术后月经恢复时间早于未成功组（P＜0.05）；未成功组术后1个月、术后2个月FSH/LH高于成功组，HOXA11、PAPP-A低于成功组（P＜0.05）；术后月经恢复时间、术后1个月FSH/LH、HOXA11、PAPP-A均与再妊娠结局相关（P＜0.05）;FSH/LH、HOXA11、PAPP-A的AUC分别为0.754、0.801、0.767，三者联合的AUC为0.928;FSH/LH、HOXA11、PAPP-A高水平患者未成功危险度是低水平患者的2.158、0.299、0.446倍（P＜0.05）。结论 FSH/LH、HOXA11、PAPP-A与CSP患者子宫动脉栓塞术后再妊娠结局有关，联合检测时可为临床预测再妊娠结局提供可靠参考，从而为临床早期干预提供参考。

Background: A major concern about the Laparoscopy Approach to Cervical Cancer trial is the disparities in laparoscopic radical hysterectomy experience between the participating centers and the potential effects of the learning curve of minimally invasive surgery on the oncologic outcomes of patients. Thus, it is necessary to assess the survival of cervical cancer patients undergoing laparoscopy in a minimally invasive gynecology center. Methods: A consecutive series of patients undergoing first laparoscopic radical hysterectomy (LRH) for cervical cancer from May 2008 to December 2017 at a national laparoscopic training center were retrospectively analyzed. The overall survival (OS) and progression-free survival (PFS) were compared between groups. Results: In total, 1316 women with FIGO (2009) stage IA-IIB cervical cancer received LRH. Among them, 1114 (84.7%) were followed-up for 3 months or longer; the median follow-up period was 48 months (range, 3-144 months). In patients with stage IA, IB1 (≤ 2 cm), IB1 (> 2 cm), IB2, IIA1 and IIA2-IIB tumors, the 4-year PFS rates were 98.6%, 94.5%, 87.4%, 65.6%, 80.0% and 67.4%, respectively, and the 4-year OS rates were 98.6%, 96.8%, 91.1%, 77.4%, 85.6% and 76.2%, respectively. The 4-year PFS and OS were as high as 96.2% and 97.5%, respectively, in patients with squamous cell carcinoma of 2 cm or smaller in diameter. A stable high 4-year OS and PFS was achieved after completing 100 LRHs. In patients operated on by the same surgeon, an improvement in survival was observed after 40 LRHs. Conclusion: Favorable oncologic outcomes can be achieved in patients with IA-IB1 cervical cancer after LRH in a center with a high surgery volume.

目的 探讨高危型人乳头瘤病毒(HR-HPV)宫颈上皮内瘤变(CIN)患者术后微小RNA-145(miR-145)、miR-135a-5p、miR-365的表达与HR-HPV转归的关系及预测价值.方法 选取2019年2月至2021年3月武汉市红十字会医院和华中科技大学附属协和医院行手术治疗HR-HPV CIN患者98例,根据HR-HPV感染转归分为转阴组(n=48)、未转阴组(n=50).比较2组一般资料、miR-145、miR-135a-5p、miR-365表达;采用多因素Logistic回归分析术后HR-HPV感染转归的影响因素;采用受试者工作特征曲线(ROC)及ROC下面积(AUC)分析miR-145、miR-135a-5p、miR-365预测术后HR-HPV感染转归的价值.结果 未转阴组miR-145、miR-365表达低于转阴组,miR-135a-5p表达高于转阴组(均P<0.05);miR-145、miR-365是转阴的保护因素,miR-135a-5p是转阴的危险因素(均P<0.05);miR-145、miR-135a-5p联合miR-365预测HR-HPV转阴的AUC(0.925)大于单独的miR-145(0.769)、miR-135a-5p(0.800)、miR-365(0.799);miR-145、miR-365高水平者持续感染风险分别是低水平者的0.449倍、0.465倍,miR-135a-5p高水平者持续感染风险是低水平者的3.712倍(均P<0.05).结论 CIN术后miR-145、miR-365表达降低和miR-135a-5p表达升高与HR-HPV转归密切相关,三者联合检测可能是早期预测HR-HPV感染转归的有效方法.

Objectives: We aimed to identify the risk factors associated with pelvic lymph node metastasis (LNM) at each anatomic location in patients with stage IB1 cervical cancer. Methods: A primary cohort of 728 patients with stage IB1 cervical cancer who underwent radical hysterectomy and systematic pelvic lymphadenectomy were retrospectively studied. All removed pelvic nodes (N=20,134) were pathologically examined. The risk factors for LNM in different anatomic regions (obturator, internal iliac, external iliac, and common iliac) were evaluated by multivariate logistic regression analyses. Nomograms were generated from the primary cohort and validated in another external cohort (N=242). The performance of the nomogram was assessed by its calibration and discrimination. Overall survival and progression-free survival in patients with different LNM patterns were compared. Results: LNM was found in 266 (1.3%) removed nodes and 106 (14.6%) patients. The incidences of LNM at the obturator, internal iliac, external iliac, common iliac, and parametrial regions were 8.5%, 5.4%, 4.7%, 1.9% and 1.8%, respectively. Among others, tumour size and lymph-vascular space invasion (LVSI), which are preoperatively assessable, were identified as independent risk factors of LNM in the common iliac region and the lower pelvis, respectively, and age was an additional independent risk factor of obturator LNM. The negative predictive values of tumour size <2 cm for common iliac LNM and negative LVSI combined with older age (> 50 years) for obturator LNM were 100% and 98.7%, respectively. A nomogram of these two factors showed good calibration and discrimination (concordance index, 0.761 in the primary cohort and 0.830 in validation cohort). The patients with common iliac LNM had poorer survival than those with LNM confined to the lower pelvis, while the differences in survival between patients with LNM confined to one node, one region or single side and those with more widely spreading LNM were not statistically significant. Conclusions: Tumour size, LVSI and age are region-specific risk factors for pelvic LNM in IB1 cervical cancer, which could be used to allocate the appropriate extent of pelvic lymphadenectomy.

目的:研究闭孔神经横断特点,为预防及处理闭孔神经横断提供参考.方法:回顾性分析2018年11-2020年11月期间发生的2例因妇科恶性肿瘤行盆腔淋巴结清扫术中闭孔神经横断的病例资料.结果:2例闭孔神经横断分别发生在入骨盆处和髂总静脉分叉处,经术中立即修补及术后对症治疗,术后3个月同侧股内收肌功能障碍及神经感觉缺失的症状均完全消失.结论:术中及术后积极处理闭孔神经横断,预后良好.

Decreased miR-335 has been reported in a variety of cancers. We previously showed that miR-335 played an important role in ovarian cancer metastasis and prognosis. However, miR-335 is down-regulated in ovarian cancer by mechanisms that remain unclear. In silico analysis identified putative transcription factor specificity protein 1 (SP1) transcription factor binding sites in the miR-335 promoter. To investigate the relation between SP1 and miR-335, qRT-PCR was performed. Our results showed both Sp1 knockdown and mithramycin A increased miR-335 expression in ovarian cancer cell lines. Luciferase reporter assays indicated that Sp1 knockdown increased miR-335 transcriptional activity. ChIP experiments showed that Sp1 bound directly to miR-335 promoter. Moreover, transwell migration and wound-healing assays showed that Sp1 knockdown resulted in inhibited cell migration, which was in turn mitigated by miR-335 inhibitor. We propose that miR-335 was negatively regulated by SP1, which in turn contributes to miR-335 deregulation and tumor cells migration.

Objectives Lymph node metastasis (LNM) is an important determinant of prognosis in patients with cervical cancer. Members of the angiopoietin family have been demonstrated to regulate tumor-associated angiogenesis and lymphangiogenesis. This study aimed to investigate the expression levels of angiopoietin-1 (ANG1) and angiopoietin-2 (ANG2) in clinically early stage of cervical cancer along with their correlations with LNM. Methods In total, 124 human cervical cancer cases classified into stage IA-IIB in accordance with the International Federation of Gynecology and Obstetrics (FIGO) 2009 staging criteria were included. ANG1 and ANG2 expression levels in the tumor sections were assessed by immunohistochemistry (IHC). Univariate and multivariate logistic regression models, including age at diagnosis, FIGO stage, tumor size, pathological type, histological grading, depth of stromal invasion, lymph-vascular space invasion (LVSI) and the expression status of ANG1 and ANG2, were used to evaluate the odds ratios (ORs) for LNM. Results ANG1 and ANG2 were positively expressed in 75 (60.5%) and 89 (71.8%) cervical cancers respectively, with predominant staining in the cytoplasm. ANG1 expression was significantly decreased in tumors with LNM, while no correlation was observed between ANG2 expression and LNM. More importantly, the multivariate logistic regression analysis demonstrated that high ANG1 expression was an independent protective factor of LNM (OR 0.107, 95% confidential interval [CI] 0.020~0.567), while LVSI was an independent risk factor of LNM (OR 34.313, 95% CI 5.914~199.092). Conclusion ANG1 is associated with a significantly decreased risk of LNM in early stage cervical cancer. The predictive value and role of ANG1 in LNM needs to be further investigated in future studies.

The present study aimed to investigate the implication of long non-coding RNA (lncRNA) expression profiles in post-menopausal osteoporosis (PMOP). A total of 10 patients with PMOP and 10 age-matched healthy post-menopausal females as controls were consecutively enrolled. Their peripheral blood mononuclear cells were obtained and lncRNA as well as mRNA expression profiles were detected by RNA sequencing, followed by bioinformatics analyses. The lncRNA expression profiles were able to distinguish patients with PMOP from controls based on principal component analysis and heatmap analysis. In total, 254 upregulated lncRNAs and 359 downregulated lncRNAs were identified in patients with PMOP vs. controls. The top 5 upregulated lncRNAs were RP11-704M14.1, RP11-754N21.1, RP11-408E5.5, ANKRD26P3 and TPTEP1. The top 5 downregulated lncRNAs were RP11-310E22.4, RP11-326K13.4, FABP5P1, SERPINB9P1 and RPL13P2. Based on the interaction of dysregulated lncRNAs and mRNAs by RNA sequencing, functional annotations were then performed. Gene Ontology enrichment analysis revealed that the dysregulated lncRNAs were enriched in terms including apoptotic process and positive regulation of NF-kappa B transaction, and Kyoto Encyclopedia of Genes and Genomes analysis suggested enrichment in PMOP-associated signaling pathways, including osteoclast differentiation, tumor necrosis factor signaling pathway and mitogen-activated protein kinase signaling pathway. In addition, the regulatory network and circos graph further indicated the implication of lncRNA expression profiles in PMOP via interactions with mRNAs. In conclusion, the present study suggested that aberrant lncRNA expression is deeply involved in the pathogenesis of PMOP by affecting osteoclast differentiation, inflammation and apoptotic processes.

PURPOSE:The HOX transcript antisense RNA (HOTAIR) has been reported to be aberrantly expressed in ovarian cancer (OC). Abnormal high expression level of HOTAIR has been found to be associated with poor overall survival of OC patients. Yet, the role of HOTAIR in paclitaxel resistance of OC is unclear. This study aims to investigate the effect, as well as the mechanism of HOTAIR in promoting paclitaxel resistance of OC.METHODS:Ovarian cancer cell lines with down-regulated and up-regulated expression of HOTAIR were, respectively, established. The expression of HOTAIR was confirmed by qRT-PCR. The sensitivity of ovarian cancer cells to paclitaxel was detected by MTT assays, colony formation, EdU assays, flow cytometry, and in vivo experiments.RESULTS:An increased expression level of HOTAIR was observed in ovarian cancer cell lines following treatment with paclitaxel. When the expression of HOTAIR was down-regulated, the proliferation of ovarian cancer cells was found to be inhibited, coupled with enhanced cell sensitivity to paclitaxel. Conversely, when the HOTAIR expression was up-regulated, an opposite effect was observed on the ovarian cancer cells. In addition, cell cycle arrest in G2/M phase was also shown to be accelerated upon HOTAIR suppression. Strikingly, our results also revealed that HOTAIR plays a regulatory role in the expression of checkpoint kinase 1 (CHEK1), and that the restored paclitaxel sensitivity through knockdown of HOTAIR can be weakened by CHEK1 up-regulation. Consistently, in vivo data confirmed that the therapeutic efficacy of paclitaxel can be enhanced through down-regulation of HOTAIR, and that CHEK1 is the down-stream target of HOTAIR in inducing paclitaxel resistance.CONCLUSION:HOTAIR confers paclitaxel resistance in epithelial ovarian cancer by increasing the protein level of CHEK1.

MicroRNA (miRNA/miR‑126) has been shown to be associated with ovarian cancer in previous studies. In ovarian cancer, however, the specific status of miR‑126 remains largely unknown. In the present study, to clarify its role in ovarian cancer, the levels of miR‑126 were first examined using laser microdissection and RT‑qPCR. It was found that the miR‑126 level was decreased in ovarian tissue samples and the restoration of miR‑126 inhibited cell proliferation, cell invasion and migration in vitro and suppressed tumor growth in vivo. A bioinformatics search revealed that the angiogenesis‑related gene, vascular endothelial growth factor (VEGF)‑A, was among the potential targets of miR‑126. The suppression of invasion and proliferation induced by ectopic miR‑126 expression was nullified by the ectopic expression of VEGF‑A, suggesting that these suppressive effects were largely attributable to the ability of miR‑126 to target VEGF‑A. Moreover, the restoration of miR‑126 suppressed the angiogenic potential of human umbilical vein endothelial cells (HUVECs). On the whole, these findings indicate that the loss of expression of miR‑126 contributes to the abnormal VEGF‑A accumulation and subsequent unchecked cell invasion and cell proliferation in epithelial ovarian cancer.

Background: Primary gastrointestinal stromal tumor (GIST)-mimicking gynecologic masses are easily misdiagnosed. This study aimed to investigate the clinicopathological features, management, and prognosis of primary GIST mimicking as gynecologic mass. Methods: Clinicopathological and survival data of GIST mimicking as gynecologic mass admitted to our center from January 2005 to December 2017 were retrospectively analyzed. Results: Thirty-eight patients were included. The most common primary tumor site was the jejunoileum (n = 33, 86.9%), and 33 patients (86.9%) were classified as high recurrence risk. The short-term outcomes of operative incision length (P = 0.004), time to gas passage (P = 0.002), and hospital stay (P = 0.012) with laparoscopy-assisted resection were significantly shorter than those with open resection, showing no significant differences of long-term outcomes. With a median follow-up of 56 mo for 31 patients (81.6%), 18 (58.1%) received adjuvant therapy with imatinib. The 5-year disease-free survival and disease-specific survival of pelvic high-risk GIST was 37.0% and 48.3%, respectively; both were significantly lower than those of the other female high-risk group (both P < 0.05). Conclusions: GIST mimicking as gynecologic mass is not uncommon, most originate from the jejunoileum and have a high risk of recurrence. Laparoscopy-assisted resection may be preferable for more favorable short-term outcomes. Compared with the other female high-risk GIST, pelvic high-risk GIST has a significantly worse prognosis; a longer duration of adjuvant therapy with imatinib than recommended may be beneficial. (C) 2019 The Author(s). Published by Elsevier Inc.

The utility of placental growth factor (PlGF) and its receptor VEGFR-1 (Flt-1) as biomarkers for cervical cancer has not been clarified yet. To address this issue, we investigated the levels of soluble PlGF (sPlGF) and soluble Flt-1 (sFlt-1) in the serum from patients with early cervical cancer, cervical intraepithelial neoplasia (CIN) and controls in this study. sPlGF and sFlt-1 were detected in 44 preoperative patients with cervical cancer, 18 cases with CIN, and 20 controls by ELISA. It was found that both sPlGF and sFlt-1 were significantly increased in the cervical cancer group as compared with those in CIN and control groups. sPlGF presented a high diagnostic ability of cervical cancer, with a sensitivity of 61.36% and a specificity of 89.47%; and sFlt-1 with a sensitivity of 50.00% and a specificity of 92.11%. Importantly, the combined use of sPlGF and sFlt-1 could increase the diagnostic rate of cervical cancer, with a sensitivity of 70.45% and a specificity of 92.11%. These results indicated that both sPlGF and sFlt-1 in circulation can serve as possible valuable diagnostic biomarkers for cervical cancer, and the combined use of them can be more valuable to diagnose the patients with early cervical cancer.

目的 检索、评价和汇总宫颈癌患者根治术后尿潴留的预防和管理的最佳证据,为临床护理工作提供参考. 方法 计算机检索BMJ Best Practice、UpToDate、Cochrane图书馆、Joana Briggs Institute循证卫生保健国际合作中心数据库、OVID循证数据库、中国指南网、美国指南网、ClincalKey for Nursing、加拿大安大略注册护士协会、PubMed、荷兰医学文摘数据库、护理文献累积索引数据库关于宫颈癌患者根治术后尿潴留预防及管理的所有证据,包括临床实践指南、最佳临床实践信息册、证据汇总及系统评价.检索时限从建库至2018年5月. 结果 共纳入16篇文献,包括临床实践指南5篇、系统评价5篇、证据总结4篇、临床决策2篇,最终获取14条最佳证据.结论 临床科室在应用证据时,应结合文化背景、具体的临床环境、患者意愿及偏好等有针对性地选择证据,及时关注相关证据的更新.

Objective:To analyze the reasons of related organs injuries in laparoscopic radical surgery for cervical cancer and investigate the methods to reduce the related organs injuries.Methods:We retrospectively analyzed the data of 288 cases of patients with cervical cancer who received the laparoscopic in our hospital from January 2010 to January 2016.According to the statistics of the incidence rate of intestinal injury,urinary organ injury,obturator nerve injury and vessels injury in the operations,we analyzed the possible reasons for these injuries and investigated the treatment and prevention approaches as well as experience during or after the operations.Results:In the 288 cases of patients with laparoscopic radical surgery for cervical cancer,there were 10 cases of intestinal injury,2 cases of obturator nerve injury,22 cases of urinary organ injury and 5 cases of vessels injury.The incidence rate was respectively 3.5％,0.6％,7.6％ and 1.7％.The intes-tinal injury was cured by suture repair during the operation or by the secondary operation.Obturator nerve injury had a good prognosis by repair during the operation in time.The urinary organs injury was cured by anastomosis,leaving ureteral stent or catheterization during or after the primary operations.The vessels injury was cured by hemostasis during the primary operations or secondary operations.Conclusion:The organs injuries of laparoscopic radical surgery for cervical cancer may be related to the diseases themselves and experience of the doctors.The full preoperative preparation,standard operation and close observation contribute to reduce the injuries of related organs and the occurrence of severe consequences,improve the clinical curative effect and prognosis of the patients.

The present study was performed to identify and characterize genes involved in osteoblasts function. Firstly, we constructed and sequenced a human osteoblast full-length cDNA library to screen for genes whose functions have not been reported and further identify these candidate genes through detecting the relationship with the activator protein-1 (AP-1) transcription factor complex using a dual luciferase reporter system. Only one gene, namely METRNL (Meteorin, glial cell differentiation regulator-like) has been screened out. We performed immunohistochemistry to analyze expression patterns in bone and established a stable transfection MG63 cell line of METRNL-EGFP fusion protein overexpression to analyze the function of METRNL in mineralized nodule formation. Immunohistochemistry showed METRNL expression in hypertrophic chondrocytes and osteoblasts lining trabecular bone surfaces. Overexpression of METRNL inhibited mineralized nodule formation by the MG63 osteosarcoma cell line. Thus, the identified gene, METRNL, which is associated with AP-1 transcription factor complex activity, has a unique expression pattern in bone. In addition, the anomalous expression of METRNL may inhibit bone cell differentiation.

目的 评估绝经过程、年龄和其他相关因素对北京市社区妇女生活质量的影响.方法 招募北京市某社区35～ 64岁健康女性,运用中国版绝经期特异性生活质量量表(Menopause-Specific Quality of Life Questionnaire,MEN-QOL)问卷获取数据.妇女按年龄每5岁分成一组,并同时分为生育期、绝经过渡期和绝经后期,以评价绝经状态和年龄及其他相关因素对生活质量的影响,并分析不同绝经状态之间生活质量的差异.结果 在同一年龄段,生活质量从生育期、绝经过渡期到绝经后期呈逐步下降趋势.在绝经过渡期和绝经后期妇女中,血管舒缩症状和躯体领域的生活质量下降显著;绝经后期妇女的心理和性领域生活质量下降显著.随着绝经的进展,性领域的生活质量逐渐下降,但绝经后期早期血管、心理和躯体三方面的生活质量均最差.年龄与血管舒缩领域、性领域生活质量均呈负相关(标准参数分别为0.12、0.19,P均＜0.01).评价自我健康状态为一般或较差的妇女生活质量差于自我评价健康状态为好的妇女(P＜0.05).体重指数高的妇女血管舒缩症状更严重(P＜0.05).结论 绝经是对北京社区妇女的生活质量产生负面影响的独立因素,妇女的生活质量随着绝经的到来发生某些规律性的改变.也应考虑年龄和其他因素对生活质量的影响.

Objective:To explore the role of chemokine receptor 4(CXCR4) and its ligand stromal derived factor-1α(SDF-1α) in the processes of adhesion and invasion of ovarian cancer cells through activating the mitogen-activated protein kinases(MAPK) signaling pathway and its possible mechanism.Methods:Intracellular calcium mobilization in SKOV3 cells was detected with a laser scanning confocal fluorescence microscopy before and after SDF-1α treatment.Western blotting was used to detect the phosphorylation of extracellular signal-regulated kinase(ERK1/2) in SKOV3 cells after exposure to SDF-1α.Adhesion capability and matrix metalloproteinase(MMP) activity of ovarian cancer cells after exposure to SDF-1α were mesured by adhesion assay and gelatin zymography,respectively.Results:SDF-1α induced rapid intracellular calcium mobilization in a metastatic ovarian cancer cell line SKOV3,as well as rapid phosphorylation of ERK-1/2.Adhesion capability of ovarian cancer cells to fibronectin(FN) and collagen Ⅳ(COL) was increased by SDF-1α treatment,while the addition of PD98059,an inhibitor of ERK-1/2 signaling,reduced the effects of SDF-1α.SDF-1α also increased the activities of MMP-2 and MMP-9 secreted by SKOV3 cells.Conclusion:SDF-1α/CXCR4 regulates adhesion ability of ovarian cancer cells by activating MAPK signaling pathway and stimulates secretion of MMP-2 and MMP-9,thereby participates the processes of invasion and metastasis of ovarian cancer.

This study was aimed to explore the role of stromal-derived factor 1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis in mediating the metastasis of ovarian cancer cells through activation of extracellular signal-regulated kinase-1/2 (ERK-1/2) signaling pathway. A highly metastatic ovarian cancer cell line, SKOV3, was used in the study. Intracellular calcium mobilization was detected by using laser scanning confocal fluorescence microscopy. Western blotting was used to detect the phosphorylation of ERK1/2 in SDF-1α-treated SKOV3 cells. Adhesion capability and matrix metalloproteinase (MMP) activity of ovarian cancer cells after exposure to SDF-1α were measured by adhesion assay and gelatin zymography. The results showed that SDF-1α induced rapid intracellular calcium mobilization in SKOV3 cells, as well as the phosphorylation of ERK-1/2. The adhesion of ovarian cancer cells to fibronectin and collagen IV was increased after SDF-1α treatment. An inhibitor of ERK-1/2 signaling, PD98059, could antagonize such effects of SDF-1α. SDF-1α could also increase the secretion of active MMP-2 and MMP-9. It was concluded that the SDF-1/CXCR4 axis played a critical role in the metastasis of human ovarian cancer by increasing the adhesion capability of cancer cells and the activity of MMP-2 and MMP-9 via ERK1/2 signaling pathway.

Objective:To study the expression of placental growth factor(PLGF) in precancerous lesions of cervical cancer and early invasive cervical squamous cancer and explore the relationship between the expression of PlGF and microvessel density(MVD),lymphatic vessels density(LVD) as well as its clinical significance.Methods:Immunohistochemical staining and RT-PCR were used to detect the protein and mRNA expressions of PLGF in 22 pre-cancerous lesions and 36 early invasive cervical squamous cancer specimens.CD34 and D2-40 related immunohistochemical methods were used to determine the MVD and LVD levels.The association of PLGF expression with MVD and LVD levels was analyzed.Results:The positive rate of PLGF protein was 22.73%(5/22) in pre-cancerous lesions and 58.33%(21/36) in early invasive cervical squamous cancer tissues.Its expression was significantly related with the clinical staging(P0.01).The mRNA expression of PLGF was consistant with its protein expression.There was a positive correlation between expression of PLGF and MVD and LVD(P0.01,P0.05).Conclusions:Both mRNA and protein expression levels of PLGF in precancerous lesions of cervical cancer were related to its clinical staging and tumorigenesis.PLGF has the potential to become a new target for neoadjuvent chemotherapy for cervical cancer.