环介导等温扩增(LAMP)技术是一种恒温核酸扩增方法,具有较强特异性,并且操作快速简单,具有较高的灵敏度.目前,此技术被广泛应用到多方面,并且具有一定的改进,主要为多重环介导等温扩增技术实现及环介导等温扩增技术和其他技术的联合使用.该文对环介导等温扩增技术在食品检测中的应用进行分析,从而为环介导等温技术的发展及应用提供参考.

冠突散囊菌具有独特的功能与生理特性,随着现代生物检测技术的不断优化,冠突散囊菌的安全检测、基因研究也越发自如.为提高冠突散囊菌在发酵茶中的应用效益,还需加强对微生态学和发酵工程的研究,以更好的改善发酵茶品质,以及发挥其保健功效.

利用环介导等温扩增技术建立肠炎沙门菌快速检测方法.根据肠炎沙门菌SEN1393基因特异序列设计引物,用凝胶电泳测试实验结果,再优选反应体系,最后检测牛乳样品中肠炎沙门菌.试验结果显示该方法能够在60℃条件下1h内检出肠炎沙门菌,对5株沙门菌和27株非沙门菌检测,只有肠炎沙门菌可以检测出阳性扩增,所设计的引物有良好的特异性;DNA检测灵敏度为94 fg/μL.该方法具有高效、特异性强和敏感性高等特点,可满足肠炎沙门菌快速检测筛选的要求.

目的：分析高职高专护理类专业病原生物与免疫学教材编写成败得失。方法以3种高职高专护理类专业病原生物与免疫学教材为分析样本，在近3届学生和教师中开展调查研究，对其结果进行分析、探讨。结果有100%的教师、超过90%的学生认为3本教材的“免疫学”部分均难度太大、内容太多、语言太抽象；超过85%的师生认为3本教材知识量过大。结论在高职高专病原生物与免疫学教材编写过程中，应尽量使教材的知识量、语言、编排顺序、附录等适应教学实际，以便于教师教学和学生学习。

Purpose: A loop-mediated isothermal amplification(LAMP) method was established for the detection of Alicyclobacillus acidoterrestris in foods. Methods: After optimization of the reaction conditions of LAMP including the concentrations of primers, reaction time and amplification temperature, the LAMP method was developed, and its sensitivity and specificity were evaluated. Results: The method was capable of rapidly and specifically detecting A. acidoterrestris in foods within 1 hour at a constant temperature of 63 ℃. The sensitivity of the method was 6.7 CFU/m L and the specificity was 100%. Conclusions: The LAMP method is efficient, highly sensitive and specific, and suitable for the rapid detection of A. acidoterrestris in various food samples.

Objective:To study success and failure to write a new teaching material Pathogenic Biology Immunology about nursing specialities of medical institutions of higher learning.Methods:Used three kinds new teaching material Pathogenic Biology Immunology about nursing specialities of medical institutions of higher learning as sample book,fact-finding results in recent three years′students and teachers were analysed.Results:100% of the teachers and 93%-97% of the students thought that immunology part was too hard,numerous and abstract.89%-96% of the teachers and stu-dents thought that knowledge volume of three copies new teaching materials were all too large or a bit too large,and Zhao ′s was specially large.Conclusion:Teaching material′s knowledge volume,words of writing,in order to write,appendix and others important things must be specially paid attention when we write a new teaching material Pathogenic Biology Im-munology about nursing specialities of medical institutions of higher learning,so that new teaching material having been written is still more practical and perfect.