Post-stroke depression (PSD) poses a serious impact on patients' life quality. Effective drugs to treat this annoying disease are still being sought. Yi-nao-jie-yu (YNJY) prescription has been found to relieve PSD; however, the underlying mechanisms remain unelucidated. This work elucidated the therapeutic effects and mechanisms underlying YNJY prescription in PSD. PSD rat model was treated with YNJY prescription and ML385. Depression-like behaviors of rats was appraised. Hematoxylin-eosin, Nissl, and NeuN immunofluorescence staining were performed to observe hippocampal neuronal damage. Transmission electron microscopy was used to assess hippocampal mitochondrial damage. Commercial kits and western blotting were adopted to research ferroptosis-related factors and Nrf2/GPX4/SLC7A11 signals. In vitro experiments were performed using rat hippocampal neurons to explore the mechanism by which YNJY prescription relieves PSD. In PSD rats, YNJY prescription relieved depression-like behaviors, attenuated hippocampal neuronal damage, mitigated hippocampal ferroptosis and mitochondrial damage, and activated hippocampal Nrf2/GPX4/SLC7A11 pathway. By in vitro experiments, erastin treatment exacerbated hippocampal neuronal damage, ferroptosis, mitochondrial damage, and lipid peroxidation; however, YNJY prescription abolished these erastin-induced effects. In the erastin-treated hippocampal neuronal model of PSD, ML385 treatment increased ferroptosis, hippocampal neuronal damage, and lipid peroxidation; however, YNJY prescription counteracted these ML385-induced effects. By in vivo study, ML385 reversed the relief of YNJY prescription on depressive-like behaviors of PSD rats, and the inhibition on ferroptosis in PSD rats' hippocampus. YNJY prescription relieves PSD by blocking ferroptosis via activating the Nrf2/GPX4/SLC7A11 pathway. It may be a promising agent for treating PSD clinically.

黄元御"一气周流"理论指在中央土气斡旋下,左路木火之气升发,右路金水之气敛降,从而形成气机在人体内"如环无端,周流不息"的运行状态,其中任何环节出现问题都会导致气机周流不畅,百病乃生.轻度认知功能障碍(MCI)属中医学"健忘"范畴,其病因病机错综复杂,涉及多脏腑多经络,与气血津液密切相关.本研究应用"一气周流"理论,立足于整体,将MCI的病机概括为中土不运、肝木不疏、肾水不温3 个方面,治以培植中土、疏达肝木、温煦肾水,并以黄元御的核心方剂为基础加减治疗,结合临床典型案例阐释,以期为中医临床防治MCI拓宽思路.

目的:基于数字图像处理技术探讨针刺治疗阈下抑郁的舌象变化及临床疗效.方法:选择就诊于北京中医药大学第三附属医院门诊的阈下抑郁患者50例,最终共48例完成观察(脱落2例),采用自身前后对照的方法,给予颐神调气法针刺治疗,留针30 min,隔日1次,共治疗4周.采用TFDA-1型舌诊仪采集患者治疗前后的舌象,分析舌色、舌苔在RGB、Lab、HSV 3种颜色空间下的9种特征的变化规律,并通过评测蒙哥马利抑郁量表(montgomery depression scale,MADRS)的评分观察针刺的临床疗效.结果:治疗4周后,舌色分析显示:在不同颜色空间中,舌色R、a、S、V值较治疗前明显升高(P<0.05),提示舌色在针刺治疗后明显红润、明亮;舌苔分析显示:在不同颜色空间中,舌苔R、a、H、S、V值治疗后明显升高(P<0.05),提示经治疗后舌苔由厚变薄,舌苔颜色无明显变化;蒙哥马利抑郁量表评分显著低于治疗前(P<0.01),有效率为81.25％.结论:针刺治疗阈下抑郁具有良好的临床疗效,并可通过分析治疗前后舌色、舌苔客观化指标的明显变化得到验证.

目的:观察颐脑解郁方对抑郁大鼠行为学和NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein do-main associated protein 3,NLRP3)相关炎性蛋白的影响.方法:采用随机数字表法将32 只雄性Wistar大鼠随机分为正常组、模型组、颐脑解郁方组(6.2g·kg-1)和盐酸氟西汀组(2.3g·kg-1),每组8 只.除正常组外,其余大鼠采用慢性不可预知温和刺激(chronic unpredictable mild stress,CUMS)法连续刺激21d联合孤养法建立抑郁模型,并于应激结束后给予相应药物干预28 d,正常组和模型组灌胃等体积蒸馏水,灌胃体积均为 10 mL·kg-1.采用旷场实验和糖水偏好实验评估各组大鼠的行为学变化;ELISA法检测大鼠血清中白细胞介素-1β(interleukin 1β,IL-1β)、干扰素-γ(interferon γ,IFN-γ)的水平;RT-PCR检测海马和前额叶皮质组织中NLRP3 mRNA的表达量;Western Blot检测海马和前额叶皮质中NLRP3 和gasdermin D(GSDMD)的蛋白表达水平.结果:与正常组比较,模型组大鼠糖水偏好率、活动总路程、水平运动得分和垂直运动得分显著降低,血清IL-1β、IFN-γ水平及海马和前额叶皮质中NLRP3 mRNA、NLRP3 蛋白以及GSDMD蛋白表达水平显著升高;与模型组比较,颐脑解郁方组大鼠糖水偏好率、活动总路程、运动时间、水平运动得分和垂直运动得分显著升高,静止时间显著减少,血清IL-1β、IFN-γ水平及海马和前额叶皮质中NLRP3 mRNA、NLRP3 蛋白以及GSDMD蛋白表达水平下降,差异均具有统计学意义(P<0.05).结论:颐脑解郁方可能通过抑制海马和前额叶皮质中NLRP3 的激活抑制下游GSDMD和炎症因子IL-1β、IFN-γ的成熟和释放,进而改善大鼠的抑郁样行为.

目的 观察清肝化痰方治疗高血压前期(prehypertension,PHT)的临床疗效.方法 招募健康体检人群中PHT(BP:120～139 mm Hg/80～89 mm Hg)受试者60例.以随机数字表法分为观察组及对照组各30例,对照组实际观察27例(3例脱落),给予健康宣教,引导改善生活方式;观察组实际观察28例(2例脱落),在对照组基础上给予清肝化痰方治疗.共治疗4周,并以治疗前后的诊室血压、中医证候量表及动脉粥样硬化性心血管疾病(atherosclerotic cardiovascular disease,ASCVD)发病风险预测模型(China-PAR)进行疗效评价.结果 与干预前相比,干预后两组受试者的中医证候积分、收缩压(systolic blood pressure,SBP)水平及观察组的舒张压(diastolic blood pressure,DBP)水平、ASCVD十年风险与终身风险均有降低(P＜0.01,P＜0.05);观察组干预后的中医证候积分、SBP、DBP及ASCVD十年风险均低于对照组(P＜0.01,P＜0.05).观察组血压值疗效及中医证候积分疗效分别为64.29％和82.14％,对照组分别为22.22％和37.03％,观察组总体疗效水平明显优于对照组(P＜0.01).从具体症状上看,两组急躁易怒、头重如裹、胸闷、畏寒肢冷、口淡、口干、失眠、气短症状均有改善(P＜0.05).此外,观察组受试者的眩晕、头痛、呕吐痰涎、面赤、口苦、耳鸣症状也有改善(P＜0.01,P＜0.05),且观察组的眩晕、急躁易怒、呕吐痰涎、面赤、口干、口苦、心悸、耳鸣症状改善程度较对照组差异有统计学意义(P＜0.05).结论 清肝化痰方治疗PHT相较于单纯生活方式干预降压效果更显著,且能安全有效地改善临床症状并降低罹患ASCVD的风险.

Objective To explore the effects of Yinao Jieyu Formula（YNJYF） on pro-inflammatory factors for depression based on network pharmacology and experimental verification. Methods The ingredients of YNJYF were obtained from TCMSP and HERB databases, and the targets were detected by SwissTargetPrediction. The genes related to depression and inflammation were obtained from GeneCards database. Intersection of ingredients and disease are the potential targets of YNJYF. The network of herbs,ingredients, potential targets and diseases was built by Cytoscape 3.8.0 software. And the degree was calculated by CytoNCA to find the important active ingredients. Then the protein-protein interaction（PPI） network about the potential targets through STRING database was constructed to obtain the core proteins, and the potential targets were analyzed by GO and KEGG with the R language, then the barplot and dotplot were drawn. Finally, the more important core proteins were selected to verify by experiment.SPF male rats were randomly divided into normal group, model group, YNJYF group（Chinese medicine group） and fluoxetine group（western medicine group）. Chronic unpredictable mild stress（CUMS） and solitary rearing were used to replicate the depression model, and the depressive-like behavior was evaluated by open field experiment. Finally, the serum of each group was collected for test. Results A total of 272 active ingredients and 1167 targets of YNJYF were obtained. There were 93 targets related to depression and inflammation, and 35 potential targets of YNJYF. The important active ingredients were quercetin and caffeic acid.The core proteins were interleukin-6（IL-6）, interferon（TNF）, Toll-like receptors 4（TLR4）, interleukin-1β（IL-1β）, etc. GO and KEGG enrichment analysis showed that YNJYF had multiple targets and multiple pathways, and nuclear factor kappa-B（NF-κB）pathway was more important. Animal experiment showed that YNJYF could reduce the levels of IL-6, TNF-α and IL-1β in depressed rats. Conclusion YNJYF may play an antidepressant role by decreasing the level of pro-inflammatory cytokines in serum,which results from the joint action of multiple components, multiple targets and multiple pathways.

儒、道、佛作为源远流长的中国传统文化中占主流地位的三大主干思想体系,相辅相成地从不同角度为中医学输注养分.文章以儒、道、佛三家思想为切入点,揭示中医疾病预防调护、诊治、医德修养等内容的丰富文化内涵,加深对中国传统文化与中医医道联系的认识.

目的 观察在抗生素治疗的基础上给予蓝芩利咽解毒汤对于急性化脓性扁桃体炎的临床疗效.方法 选择80例化脓性扁桃体炎患者随机分为对照组及观察组各40例,对照组常规给予抗生素抗感染治疗,观察组在对照组基础上口服蓝芩利咽解毒汤,对比观察两组患者临床疗效.结果 观察组患者咽痛消失时间、扁桃体红肿消失时间、扁桃体脓性分泌物消失时间及体温恢复正常时间均显著短于对照组(P＜0.05);治疗后,观察组中医证候评分显著低于对照组(P＜0.05),超敏C反应蛋白(hs-CRP)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量显著低于对照组.结论 蓝芩利咽解毒汤能够有效促进急性化脓性扁桃体炎患者肿痛消退,加快退热,减轻患者临床症状,提高临床疗效,其机制可能与该方案能降低急性化脓性扁桃体炎患者炎性因子水平、抑制炎症反应有关.

目的 探讨不同浓度益气化痰活血法代表方剂调脂通脉颗粒对血脂异常痰浊阻遏证小鼠动脉粥样硬化相关炎性因子白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达的影响.方法 选取雄性ApoE基因敲除小鼠24只,均采用高脂饲料喂养方法构建血脂异常痰浊阻遏证的病-证模型.将造模成功小鼠随机分为模型组、调脂通脉颗粒低剂量组、调脂通脉颗粒中剂量组、调脂通脉颗粒高剂量组,每组6只.除模型组外,调脂通脉颗粒低、中、高剂量组分别给予2g/(kg·d)、4 g/(kg·d)、8 g/(kg·d)的调脂通脉颗粒灌胃4周.灌胃结束后,采用ELISA法检测各组小鼠血清IL-6、TNF-α水平.结果 ApoE基因敲除小鼠均无脱失.模型组、调脂通脉颗粒低剂量组、调脂通脉颗粒中剂量组、调脂通脉颗粒高剂量组小鼠血清IL-6和TNF-α水平均依次下降,差异均有统计学意义(P均<0.05);调脂通脉颗粒高剂量组血清IL-6水平均明显低于模型组、调脂通脉颗粒低剂量组、调脂通脉颗粒中剂量组(P均<0.05),血清TNF-α水平明显低于模型组、调脂通脉颗粒低剂量组(P均<0.05);调脂通脉颗粒低、中剂量组与模型组比较,调脂通脉颗粒低剂量组与调脂通脉颗粒中剂量组比较,血清IL-6和TNF-α水平差异均无统计学意义(P均>0.05).结论 调脂通脉颗粒能降低血脂异常痰浊阻遏证小鼠血清IL-6、TNF-α水平,其中高剂量调脂通脉颗粒可更明显下调IL-6水平,而TNF-α水平的降低对调脂通脉颗浓度的依赖程度不大.调脂通脉颗粒可能通过抑制动脉粥样硬化相关炎性因子的表达,从而减轻血脂异常痰浊阻遏证下血管内皮的炎症反应,延缓病变进展.

目的:观察揿针结合恢刺法治疗肾虚型腰肌劳损的临床疗效.方法:将60例肾虚型腰肌劳损患者随机分为观察组30例、对照组30例.观察组运用揿针结合恢刺的方法,隔日1次,每周3次,共4周;对照组运用普通针刺,隔日1次,每周3次,共4周.治疗结束后,分别对其疗效与安全性予以评价.结果:本研究有52例患者完成治疗,观察组27例、对照组25例.治疗完成后,两组患者疼痛视觉模拟量表评分、Oswestry功能障碍指数(ODI)、全血粘度、血浆粘度、血清TNF-α和IL-6等指标均优于治疗前,差异具有统计学意义(均P＜0.01).治疗后观察组的疼痛视觉模拟量表评分、Oswestry功能障碍指数、全血粘度、血浆粘度、血清TNF-α和IL-6等指标均优于对照组,差异具有统计学意义(P ＜0.05或P＜0.01).结论:揿针结合恢刺法可以有效治疗肾虚型腰肌劳损,且具有安全性.

目的 采用核磁技术观察抑郁大鼠大脑相关部位脑白质纤维束完整性、局部灌注情况等变化,从神经影像角度验证颐脑解郁方对抑郁症的正向调节作用.方法 根据行为学评估结果将雄性Wistar大鼠随机分为正常组、模型组、颐脑解郁方组(6.2 g/kg)、盐酸氟西汀组(2.33 mg/kg)4组,之后采用慢性不可预知温和性刺激法构建抑郁模型,造模成功后灌胃相应药物或等量蒸馏水,每日1次,共4周.灌胃结束应用扩散张量成像(DTI)、动脉自旋标记成像(ASL)方法,测定各组大鼠各向异性比值(FA)、表观扩散系数(ADC)以及血流量(CBF)值.结果 DTI显示:模型组大鼠双侧海马、前额叶皮层FA值低于正常组(P<0.05,P<0.01),ADC值高于正常组(P<0.05,P<0.01).与模型组比较,颐脑解郁方组和盐酸氟西汀组在右侧海马及双前额叶皮层FA值升高(P<0.05,P<0.01);右侧海马及左侧前额叶皮层ADC值降低(P<0.05,P<0.01).ASL显示:模型组左侧海马、双侧前额叶皮层CBF值较正常组降低(P<0.05,P<0.01),颐脑解郁方组和盐酸氟西汀组双侧前额叶皮层CBF值较模型组升高(P<0.05,P<0.01).结论 抑郁大鼠可见海马、前额叶皮层出现纤维束不同程度的损伤和微循环变化.颐脑解郁方可以通过改善神经微循环和物质代谢,修复受损的神经纤维束、恢复神经元完整、改善神经元活性及重塑能力等途径缓解或阻断抑郁症状.应用磁共振影像组学技术可较好地重现抑郁发展过程中的结构和功能变化,且具有一定的疗效评价意义.

目的 观察加味酸枣仁汤联合五行音乐治疗原发性失眠的临床疗效.方法 选择2021年1—6月就诊于北京中医药大学第三附属医院治未病中心的原发性失眠患者共66例,采用随机数字表法将其分为观察组与对照组,每组各33例.对照组实际观察30例(3例脱落),给予有中医特色的睡眠健康教育.观察组实际观察31例(2例脱落),在对照组基础上给予加味酸枣仁汤联合五行音乐治疗.治疗周期为4周,分别于治疗前、治疗后使用匹兹堡睡眠指数量表、失眠严重程度量表、过度觉醒量表、疲劳量表评价2组疗效.结果 观察组总有效率为90.32％,对照组总有效率为63.33％.2组患者的睡眠状态、失眠严重程度、过度觉醒状态、疲劳程度均较治疗前改善(P<0.01,P<0.05),且观察组在改善患者睡眠质量、入睡时间、躯体疲劳程度方面疗效优于对照组(P<0.01,P<0.05).结论 加味酸枣仁汤联合五行音乐治疗原发性失眠确有疗效,能有效改善患者睡眠质量、入睡时间、躯体疲劳,这可能与加味酸枣仁汤联合五行音乐对神经的调控作用有关.

Objectives: The objective of this study was to provide a new classification method by analyzing the relationship between urine color (Ucol) distribution and urine dry chemical parameters based on image digital processing. Furthermore, this study aimed to assess the reliability of Ucol to evaluate the states of body hydration and health. Methods: A cross-sectional study among 525 college students, aged 17–23 years old, of which 59 were men and 466 were women, was conducted. Urine samples were obtained during physical examinations and 524 of them were considered valid, including 87 normal samples and 437 abnormal dry chemistry parameters samples. The urinalysis included both micro- and macro-levels, in which the CIE L*a*b* values and routine urine chemical examination were performed through digital imaging colorimetry and a urine chemical analyzer, respectively. Results: The results showed that L* (53.49 vs. 56.69) in the abnormal urine dry chemistry group was lower than the normal group, while b* (37.39 vs. 33.80) was greater. Urine color can be initially classified based on shade by grouping b*. Abnormal urine dry chemical parameter samples were distributed more in the dark-colored group. Urine dry chemical parameters were closely related to Ucol. Urine specific gravity (USG), protein, urobilinogen, bilirubin, occult blood, ketone body, pH, and the number of abnormal dry chemical parameters were all correlated with Ucol CIE L*a*b*; according to a stepwise regression analysis, it was determined that more than 50% of the variation in the three-color space values came from the urine dry chemical parameters, and the b* value was most affected by USG (standardized coefficient β = 0.734, p < 0.05). Based on a receiver operating characteristic curve (ROC) analysis, Ucol ≥ 4 provided moderate sensitivity and good specificity (AUC = 0.892) for the detection of USG ≥ 1.020. Conclusions: Our findings on the Ucol analysis showed that grouping Ucol based on b* value is an objective, simple, and practical method. At the same time, the results suggested that digital imaging colorimetry for Ucol quantification is a potential method for evaluating body hydration and, potentially, health.

"瞑眩"反应是指患者服用药物后突然出现的一种暂时性的眩冒现象,其特点是突发性、短暂性、临床表现的多样性.此反应在患者服药过程中出现,持续时间短暂,一般在患者临床症状好转之前消失."瞑眩"反应产生机理在于阴阳之间的相互转化,是机体服用药物后正气振奋,拒邪外出,由阴阳失和的状态向阴阳平衡转化的过程.临床需将此与药物不良反应相鉴别.附验案2则以佐证.

OBJECTIVE:To investigate the effects of acupuncture on ovary morphology and function in dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) model rats. METHODS:A total of 40 adult female Wistar rats were randomly allocated to 4 groups by a random number table, including control, model, metformin and acupuncture groups, 10 rats in each group. PCOS rat model was developed by injecting with DHEA (6 mg/100 g body weight) in 0.2 mL of oil subcutaneously. Electrical stimulation (2 Hz, 3 mA) was applied to Guanyuan (CV 4), Zigong (EX-CA1) and Qihai (CV 6) acupoints for 30 min daily in the acupuncture group, and metformin (200 mg/kg) was given to rats in the metformin group, both once per day for 21 consecutive days, and rats in the normal group was fed with normal saline and fed regularly. After 21 days of administration, the rat blood samples were collected for detecting the reproductive hormonal levels [luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2), progesterone (P), testosterone (T)] and inflammatory factors (visfatin, IL-6) analysis. Ovary tissue was used for histopathological analysis. RESULTS:Compared with the model group, rats in the acupuncture and metformin groups were significantly lower in weight gain, FSH, LH and T levels, and E2 and P levels significantly increased (alll P<0.05). Meanwhile, LH and FSH levels were significantly decreased, and P, T and E2 levels significantly increased in the acupuncture group, compared with the metformin group (P<0.05). Compared with the model group, IL-6 and visfatin levels were significantly decreased in the acupuncture and metformin groups (P<0.05). There were no significant differences in IL-6 and visfatin levels between the acupuncture and metformin groups (P>0.05). Ovarian diameter in the acupuncture and metformin groups were smaller than the model group (P<0.05). However, there were no significant differences in ovarian diameters between the acupuncture and metformin groups (P>0.05). CONCLUSION:Acupuncture might improve ovary morphology and its function in DHEA-induced PCOS model rats.

目的 观察中药丹栀逍遥散对广泛性焦虑(GAD)大鼠脑Papez环路上神经营养因子[脑源性神经营养因子(BDNF)、碱性成纤维生长因子(bFGF)、神经生长因子(NGF)]及脑源性神经营养因子受体(TrkB)、碱性成纤维生长因子受体(bFGFR)表达的影响.方法 将雄性Wistar大鼠随机分为正常组、模型组、中药组(丹栀逍遥散组)和西药组(盐酸丁螺环酮组),每组8只.模型组、中药组、西药组采用天敌暴露饮水冲突刺激法建立GAD大鼠模型.中药组和西药组分别以丹栀逍遥散和盐酸丁螺环酮水溶液灌胃,每天1次,连续21 d.正常组和模型组蒸馏水灌胃21 d.造模成功后,取脑组织,行石蜡切片,免疫组织化学SP法检测Papez环路内海马(CA1、CA2、CA3、DG区)和杏仁核、丘脑前核的BDNF、TrkB、bFGF、bFGFR及NGF的表达.结果 与正常组比较,模型组海马各区和杏仁核区TrkB、NGF的表达水平明显降低(P<0.05或P<0.01);模型组海马各区bFGF、bFGFR的表达水平明显升高,杏仁核区的表达水平明显下降(P<0.05或P<0.01);模型组海马各区及杏仁核区、丘脑前核区的BDNF表达与其他3组间比较,差异均无统计学意义(P>0.05),但海马CA1区模型组与正常组有下降趋势.与模型组比较,中药组海马各区和杏仁核区bFGF、TrkB、NGF的表达水平明显升高(P<0.05或P<0.01);中药组海马各区和杏仁核区bFGFR的表达水平明显升高,西药组海马各区和bFGFR的表达水平明显降低(P<0.05);丘脑前核区的表达在模型组与正常组以及中药组与西药组之间的比较,差异均无统计学意义(P>0.05).结论 经过中药丹栀逍遥散干预的GAD大鼠,海马和杏仁核区内bFGF与其受体bFGFR,BDNF的受体TrkB和NGF的表达水平明显回升,有利于bFGF、BDNF、NGF更好地发挥其生理功能,促进损伤脑区局部微环境的恢复,增强神经可塑性,保护神经元,抑制凋亡与坏死,促进神经的修复以及神经干细胞的增殖与分化.提示疏肝清热健脾法能够通过刺激神经营养因子在海马及杏仁核内的表达来发挥其促神经修复功能.

目的 明确颐脑解郁方对血管性痴呆(vascular dementia,VD)的治疗作用,并探索颐脑解郁方改善VD认知障碍是否与调节甲状腺激素的变化相关.方法 大鼠VD模型选择改良版的线栓法造成大鼠脑血管性病变后,不予任何干预4周后经跳台实验筛选出VD模型.实验分为正常组、假手术组、VD模型组、VD中药组4组,每组大鼠15只.VD中药组大鼠灌胃中药颐脑解郁方,正常组、假手术组、VD模型组大鼠灌胃0.9％生理盐水,连续灌胃4周.灌胃1周与4周末次行跳台实验及Morris水迷宫相关检测后,每组随机选择6只大鼠麻醉处死,取下丘脑HE染色观察其病理形态.灌胃4周末次行为学检测后,随机选择8只大鼠进行腹主动脉取血,采用放射免疫法检测血清促甲状腺素(thyroid-stimulating hormone,TSH)、游离三碘甲状腺原氨酸(free triiodothyronine,FT3)、游离甲状腺素(free thyroxine,FT4)的含量.结果 跳台实验:与正常组比较,VD模型组及VD中药组大鼠灌胃1周和4周逃避潜伏期时间显著减少,错误次数显著升高(P<0.01);与VD模型组比较,VD中药组大鼠灌胃1周和4周逃避潜伏期时间明显增多,4周错误次数明显减少(P<0.05).Morris水迷宫实验:与正常组比较,VD模型组及VD中药组大鼠灌胃4周逃避潜伏期时间明显增多(P<0.05,P<0.01),VD模型组大鼠灌胃1周穿越平台次数显著下降(P<0.01),VD模型组及VD中药组大鼠灌胃4周后穿越平台次数明显下降(P<0.05);与VD模型组比较,VD中药组大鼠灌胃1周和4周逃避潜伏期时间减少,穿越次数增多(P>0.05).HE染色光镜下显示,VD中药组较VD模型组大鼠下丘脑细胞变性减轻,但仍见大片液化性坏死灶,呈筛网状结构,坏死区内神经元细胞变性、坏死,小胶质细胞增生,炎症细胞浸润,坏死边缘带窄,灌胃4周较1周大鼠下丘脑细胞变性改善明显.放射免疫法检测显示,与正常组比较,VD模型组、VD中药组FT3、FT4含量显著下降(P<0.01),TSH含量明显升高(P<0.05,P<0.01);与VD模型组比较,VD中药组FT3、FT4含量升高(P<0.05,P<0.01),TSH含量显著下降(P<0.01).结论 颐脑解郁方对血管性痴呆认知功能有改善作用,机制可能与调节下丘脑—垂体—甲状腺轴的TSH、FT3、FT4含量有关.

眼肌型重症肌无力是肌无力尚局限于上睑提肌、眼外肌、眼轮匝肌的自身免疫性疾病,鉴于现行药物及非药物治疗的不足,本文从经筋理论入手,探讨经筋理论在眼肌型重症肌无力临床上的应用.眼肌型重症肌无力的发病部位(胞睑)是各阳经经筋联属或结聚之处,首发症状与筋纵相似,病程进展与经筋病一致.在治疗上,根据眼肌型重症肌无力临床特性,宜经筋刺法调之,辅以补中升提刺法,完善了经筋理论在眼肌型重症肌无力的应用,为其诊疗提供新的思路和参考,具有重要的基础与现实意义.

目的:通过探讨血管性痴呆(VD)大鼠脑内单胺神经递质变化,研究益肾调气法对VD大鼠的干预机制.方法:选购雄性Wistar大鼠,通过旷场试验分为正常组、卒中组.MCAO法制备缺血性脑卒中模型,常规饲养28 d后经水迷宫试验筛选VD大鼠,并随机分为观察组和模型组.正常组和模型组灌胃蒸馏水,观察组灌胃颐脑解郁方,干预4周.灌胃第1、2、4周对各组大鼠行水迷宫检测;第4周灌胃后取材,采用高效液相检测大鼠右侧海马、前额叶皮质5-HT、NE、DA含量.结果:与正常组比较,模型组大鼠1、2、4周跳台潜伏期缩短、错误次数增加、水迷宫潜伏期明显延长,差异有统计学意义(P<0.01);与模型组比较,观察组第2、4周水迷宫潜伏期缩短,差异有统计学意义(P<0.01),第4周跳台潜伏期延长、错误次数减少,差异有统计学意义(P<0.01).模型组皮质及海马NE、DA、5-HT含量较正常组降低,差异有统计学意义(P<0.01),观察组海马DA含量较模型组升高,差异有统计学意义(P<0.01).结论:益肾调气法可改善VD大鼠学习记忆能力,其作用机制可能与调节大鼠脑内NE、DA及5-HT含量相关.

目的 基于对中国古代方剂的挖掘,探索防风治疗中风病中经络的作用机制.方法 检索中国中医科学院中医药信息研究所提供的中国方剂数据库有关防风治疗中风病中经络的方剂.中风病靶点信息来源于GeneCards数据库.采用频数分析挖掘方剂数据库,选取频次较高药物,借助BATMAN-TCM系统进行系统生物学分析.结果 治疗中风病中经络的方剂以防风用药频次最高;选取防风进行系统生物学预测,结果显示防风中28个化合物可通过作用于153个靶点治疗中风病,关键靶点集中在钠离子通道及乙酰胆碱相关受体亚基上,生物信息学分析结果显示其主要功能为调控离子通道或神经配体活性.结论 防风通过调控离子通道从而影响神经元电压门控,起到神经修复作用;或通过调控胆碱能受体活性,影响神经免疫功能,延缓脑损伤进程.