Gout is a prevalent form of inflammatory arthritis that occurs due to high levels of uric acid in the blood leading to the formation of urate crystals in and around the joints, particularly affecting the elderly. Recent research has provided evidence of distinct differences in the gut microbiota of patients with gout and hyperuricemia compared to healthy individuals. However, the link between gut microbiota and age-related gout remained underexplored. Our study found that gut microbiota plays a crucial role in determining susceptibility to age-related gout. Specifically, we observed that age-related gut microbiota regulated the activation of the NLRP3 inflammasome pathway and modulated uric acid metabolism. More scrutiny highlighted the positive impact of ‘younger’ microbiota on the gut microbiota structure of old or aged mice, enhancing butanoate metabolism and butyric acid content. Experimentation with butyrate supplementation indicated that butyric acid exerts a dual effect, inhibiting inflammation in acute gout and reducing serum uric acid levels. These insights emphasize the potential of gut microbiome rejuvenation in mitigating senile gout, unraveling the intricate dynamics between microbiota, aging, and gout. It potentially serves as a therapeutic target for senile gout-related conditions.

Klotho, a protein primarily expressed in the kidneys and brain, plays a critical role in aging, vascular health, and various metabolic processes. Lower serum Klotho levels have been associated with several chronic diseases, including cardiovascular disease, diabetes, and kidney disease. Although the role of Klotho in platelet regulation remains underexplored, thrombocytosis may be influenced by Klotho levels. Investigating this relationship could offer new insights into thrombocytosis pathogenesis. This study aimed to examine the relationship between serum Klotho levels and thrombocytosis in a U.S. cohort. We hypothesized that lower Klotho levels would be associated with an increased risk of thrombocytosis, potentially providing a novel perspective on thrombocytosis regulation. We conducted a cross-sectional analysis of data from 12,700 participants in the NHANES 2007-2016 cohort. Multivariate logistic regression models were used to assess the association between serum Klotho levels and thrombocytosis, adjusting for relevant covariates. Of the 12,700 participants, 86 had thrombocytosis. The thrombocytosis group had significantly lower mean serum Klotho levels compared to the non-thrombocytosis group (p < 0.01). After adjusting for confounders, an inverse association between serum Klotho levels and thrombocytosis was observed (odds ratio 0.89, 95% CI 0.82-0.97, p = 0.007). Compared to the lowest Klotho quartile (≤ 700.7 pg/ml), the adjusted odds ratios for thrombocytosis in the second (700.8-915.3 pg/ml) and third (≥ 915.4 pg/ml) quartiles were 0.6 (95% CI: 0.36-1.01, p = 0.055) and 0.49 (95% CI: 0.29-0.84, p = 0.01), respectively. Our findings suggest an inverse correlation between serum Klotho levels and thrombocytosis in adults aged 40 and older. These results highlight the potential role of Klotho in thrombocytosis regulation, and future longitudinal studies are needed to establish causality and explore the underlying mechanisms.

BACKGROUND:Antifungal resistance is increasing worldwide, which is exacerbating the serious conditions faced by the public healthcare system: there are only a few kinds of antifungal drugs. It is pressing to identify new antifungal agents and therapies to prevent and treat fungal infections, especially those caused by resistant Candida species, such as Candida glabrata. This study was to explore the antifungal effects of saponin extract from rhizomes of Dioscorea panthaica Prain et Burk (HSE, abbreviated from the commercial name Huangshanyao Saponin Extract) on the growth, biofilm formation of C. glabrata and its preliminary mechanism. METHODS:The minimum inhibitory concentration (MIC) of HSE against C. glabrata and other Candida species were evaluated following the guidelines of CLSC-M27-A3. The minimum fungicide concentration (MFC) were defined as the lowest concentration of HSE that caused no colony grown on SD agar after 48-h culture. The effects of HSE on biofilm formation and development were determined through XTT reduction assays. Confocal laser scanning microscope (CLSM) was employed to detect the influence of HSE on biofilm formation, cell membrane permeability and cell membrane potential of C. glabrata. Transmission electron microscope (TEM) was also employed to detect the ultrastructural changes caused by HSE. Checkerboard assays were performed to detect the interactions between HSE and antifungal drugs. RESULTS:HSE inhibited the growth of C. glabrata and C. albicans, as well as C. parapsilosis, with MIC of 32, 32 and 64 μg/mL, respectively. The MFC of HSE against C. glabrata and C. albicans were 32 or 64 μg/mL. While the MFC against C. parapsilosis was above 512 μg/mL. The adhesion, biofilm formation and development could also be inhibited by HSE. HSE caused cell membrane damages, as revealed by confocal microscopic analysis of PI staining and DiBAC4(3) staining. Results from TEM observation also confirmed the damages of cell membrane. In addition, HSE can synergize with AmB, while CAS and HSE can produce synergistic or additive effects. CONCLUSION:HSE showed antifungal activities against C. glabrata, as well as Candida parapsilosis, and inhibited C. glabrata biofilm formation and development. Its antifungal mechanism involves the increase in cell membrane permeability. HSE potentiated AmB and caspofungin in C. glabrata, C. parapsilosis and C. albicans. Our results suggested that HSE may be explored as antifungal agents to control Candida infections.

Abstract Background The incidence of multiple primary cancers is increasing with age and improved medical screening, but the simultaneous coexistence of solitary lung cancer and multiple colon cancers is rare. Case demonstration A 69-year-old male patient was admitted to the hospital with a physical examination that revealed a mass in the right lung and another in the hepatic flexure of the colon. Furthermore, a colonoscopy identified three masses in the colon. Therefore, the patient was diagnosed with a "pulmonary mass" along with a "colonic mass”. Following a comprehensive multidisciplinary discussion, the patient underwent a "lobectomy", and the postoperative pathology report indicated a "neuroendocrine tumor". The patient was admitted to the hospital again three months later due to abdominal pain and difficulty with bowel movements. He was diagnosed with multiple colon cancer and underwent surgery. The pathology report confirmed adenocarcinoma, and then it was confirmed to be a primary tumor originating from two different organs. Postoperative adjuvant CapeOx chemotherapy was performed after surgery. After two consecutive years of follow-up, no recurrence or metastasis of the tumor was observed. Conclusion Clinical efforts should not only focus on diagnosing one cancer but also consider the possibility of other diseases. The importance of a systematic approach to comprehensive diagnosis should be highlighted. Intraoperative colonoscopy and PET-CT have certain advantages in detecting MPCs. The treatment of MPCs should follow the principle of an individualized therapeutic approach. Tumors with a poor prognosis and higher risks should receive priority treatment.

Infections caused by Candida species, especially Candida albicans, threaten the public health and create economic burden. Shortage of antifungals and emergence of drug resistance call for new antifungal therapies while natural products were attractive sources for developing new drugs. In our study, fangchinoline, a bis-benzylisoquinoline alkaloid from Chinese herb Stephania tetrandra S. Moore, exerted antifungal effects on planktonic growth of several Candida species including C. albicans, with MIC no more than 50 μg/mL. In addition, results from microscopic, MTT and XTT reduction assays showed that fangchinoline had inhibitory activities against the multiple virulence factors of C. albicans, such as adhesion, hyphal growth and biofilm formation. Furthermore, this compound could also suppress the metabolic activity of preformed C. albicans biofilms. PI staining, followed by confocal laser scanning microscope (CLSM) analysis showed that fangchinoline can elevate permeability of cell membrane. DCFH-DA staining suggested its anti-Candida mechanism also involved overproduction of intracellular ROS, which was further confirmed by N-acetyl-cysteine rescue tests. Moreover, fangchinoline showed synergy with three antifungal drugs (amphotericin B, fluconazole and caspofungin), further indicating its potential use in treating C. albicans infections. Therefore, these results indicated that fangchinoline could be a potential candidate for developing anti-Candida therapies.

Inflammatory bowel disease (IBD) is a chronic, non-specific inflammatory condition characterized by recurring inflammation of the intestinal mucosa. However, the existing IBD treatments are ineffective and have serious side effects. The etiology of IBD is multifactorial and encompasses immune, genetic, environmental, dietary, and microbial factors. The nanoparticles (NPs) developed based on specific targeting methodologies exhibit great potential as nanotechnology advances. Nanoparticles are defined as particles between 1 and 100 nm in size. Depending on their size and surface functionality, NPs exhibit different properties. A variety of nanoparticle types have been employed as drug carriers for the treatment of inflammatory bowel disease (IBD), with encouraging outcomes observed in experimental models. They increase the bioavailability of drugs and enable targeted drug delivery, promoting localized treatment and thus enhancing efficacy. Nevertheless, numerous challenges persist in the translation from nanomedicine to clinical application, including enhanced formulations and preparation techniques, enhanced drug safety profiles, and so forth. In the future, it will be necessary for scientists and clinicians to collaborate in order to study disease mechanisms, develop new drug delivery strategies, and screen new nanomedicines. Nevertheless, numerous challenges persist in the translation from nanomedicine to clinical application, including enhanced formulations and preparation techniques, enhanced drug safety profiles, and so forth. In the future, it will be necessary for scientists and clinicians to collaborate in order to study disease mechanisms, develop new drug delivery strategies, and screen new nanomedicines.

肿瘤细胞暴露于基因组不稳定、氧化应激、营养缺乏和蛋白稳态失衡等复杂应激环境下,通过激活整合应激反应(ISR)来抵抗各种形式应激产生的损伤作用.如果ISR无法修复损伤,将通过启动死亡程序清除受损细胞.ISR介导的这种存活和死亡之间的平衡对于肿瘤的发生至关重要.目前研究认为ISR主要包括内质网未折叠蛋白反应(UPRER )和线粒体未折叠蛋白反应(UPRmt ),已经明确 UPRER和UPRmt中多个效应分子在肿瘤发生过程中的作用.近来研究表明,ISR 通过其枢纽分子 eIF2α整合UPRER和UPRmt的应激信号,eIF2α表达和磷酸化水平决定了 ISR 是促进肿瘤细胞存活还是死亡.因此,探讨ISR在肿瘤发生中的作用机制有望为肿瘤的治疗提供新靶点.

The human fungal pathogen Candida albicans can cause many kinds of infections, including biofilm infections on medical devices, while the available antifungal drugs are limited to only a few. In this study, alantolactone (Ala) demonstrated antifungal activities against C. albicans , as well as other Candida species, with a MIC of 72 μg/mL. Ala could also inhibit the adhesion, yeast-to-hyphal transition, biofilm formation and development of C. albicans . The exopolysaccharide of biofilm matrix and extracellular phospholipase production could also be reduced by Ala treatment. Ala could increase permeability of C. albicans cell membrane and ROS contribute to the antifungal activity of Ala. Overall, the present study suggests that Ala may provide a promising candidate for developing antifungal drugs against C. albicans infections.

Perineural invasion (PNI) is the process of neoplastic invasion of peripheral nerves and is considered to be the fifth mode of cancer metastasis. PNI has been detected in head and neck tumors and pancreatic, prostate, bile duct, gastric, and colorectal cancers. It leads to poor prognostic outcomes and high local recurrence rates. Despite the increasing number of studies on PNI, targeted therapeutic modalities have not been proposed. The identification of PNI-related biomarkers would facilitate the non-invasive and early diagnosis of cancers, the establishment of prognostic panels, and the development of targeted therapeutic approaches. In this review, we compile information on the molecular mediators involved in PNI-associated cancers. The expression and prognostic significance of molecular mediators and their receptors in PNI-associated cancers are analyzed, and the possible mechanisms of action of these mediators in PNI are explored, as well as the association of cells in the microenvironment where PNI occurs.

Objective:To investigate the application value of modified Overlap esophago-gastric tube (MO-EG) reconstruction in totally laparoscopic radical proximal gastrectomy.Methods:The retrospective and descriptive study was conducted. The clinicopathological data of 7 patients with upper gastric cancer or adenocarcinoma of esophagogastric junction (AEG) who underwent totally laparoscopic radical proximal gastrectomy with MO-EG reconstruction in the Second Hospital of Jilin University from January 2019 to December 2020 were collected. There were 4 males and 3 females, aged 62(range, 55-72)years. The body mass index of the 7 patients was 21.5(range, 18.5-26.0)kg/m 2. Of the 7 patients, 2 cases had early upper third gastric cancer and 5 cases had Siewert Ⅱ AEG. All patients underwent totally laparoscopic radical proximal gastrectomy with MO-EG recons-truction using barbed sutures. Observation indicators: (1) surgical situations; (2) postoperative recovery situations; (3) postoperative histopathological examinations; (4) follow-up. Follow-up was conducted using outpatient examination and telephone interview to detect postoperative esophageal reflux, endoscopic classification of esophageal reflux, anastomotic complications, tumor recurrence and metastasis and survival of patients up to December 2021. Measurement data with normal distribution were represented as Mean±SD and measurement data with skewed distribution were represented as M(range). Count data were described as absolute numbers. Results:(1) Surgical situations. All the 7 patients underwent totally laparoscopic radical proximal gastrectomy and D 1+ lymph node dissection with MO-EG reconstruction through abdominal transhiatal approach. None of the 7 patients underwent conversion to open surgery or additional thoracotomy. The operation time, time of digestive reconstruction, volume of intraoperative blood loss and length of esophagus dissected of 7 patients were (271±36)minutes, (44±10)minutes, (53±26)mL and (6.4±0.3)cm, respec-tively. (2) Postoperative recovery situations. The time to postoperative initial out-of-bed activities, time to postoperative first flatus, time to postoperative initial liquid food intake and duration of hospital stay of 7 patients were (21±5)hours, (2.9±0.9)days, (5.0±0.7)days and (10.1±1.9)days, respectively. None of the 7 patients had postoperative severe complications such as bleeding, anasto-motic leakage or mortality. One patient had postoperative pulmonary infection and recovered after anti-infection treatment. Two patients had pleural effusion and were improved after conserva-tive treatment. (3) Postoperative histopathological examinations. The tumor diameter of 7 patients was (2.5±0.7)cm. Histopathological examination of upper margins of 7 patients was negative. The distance between the esophagus margin and the superior margin of the tumor of patients with AEG was (1.8±0.6)cm. The number of lymph node dissected and the number of inferior mediastinum lymph node dissected of 7 patients were 26.0±3.6 and 3.7±1.1, respectively. Pathological TNM stages of 7 patients were 2 cases of stage ⅠB, 4 cases of ⅡA, 1 case of ⅡB. (4) Follow-up. All the 7 patients were followed up for 18(range, 12?36)months. Of the 7 patients, 4 cases reported asymptomatic, 2 cases had symptoms of reflux and 1 case had chocked feeling after eating. All the 7 patients underwent barium meal examination of gastrointestinal tract without anastomotic dysfunction or anastomotic stenosis. Six of the 7 patients underwent gastroscopy at postoperative 1 year and only 1 of them reported grade B reflux esophagitis according to Los Angeles classification, while the rest of 5 patients had no evidence of obvious reflux. None of the 7 patients had postoperative gastric cancer tumor recurrence, metastasis or death. Conclusion:Application of MO-EG reconstruction in totally laparoscopic radical proximal gastrectomy is safe and feasible, with satisfactory short-term effects.

Objective: This study was to explore the inhibitory effects of carnosol on the growth and biofilm of Candida albicans. Results: Our results showed that carnosol inhibited the planktonic growth of C. albicans with a MIC of 100 mu g/mL. Carnosol can also inhibit the biofilm formation and development of C. albicans. 25-100 mu g/mL of carnosol can obviously inhibit the yeast-to-hyphal transition in four kinds of hyphal-inducing media and the adhesion of C. albicans to polystyrene surfaces. Results from PI staining indicated that carnosol may disrupt cell membrane of C. albicans. Conclusion: Carnosol can inhibit the planktonic growth and virulence factors of C. albicans, such as biofilm formation, adhesion and hyphal growth. The antifungal mechanism may involve the increase in cell membrane permeability. (C) 2021 SFMM. Published by Elsevier Masson SAS. All rights reserved.

TMEM16A is a recently identified calcium-activated chloride channel (CaCC) and its overexpression contributes to tumorigenesis and progression in several human malignancies. However, little is known about expression of TMEM16A and its clinical significance in colorectal cancer (CRC). TMEM16A mRNA expression was determined by quantitative real time-PCR (qRT-PCR) in 67 CRC tissues and 24 para-carcinoma tissues. TMEM16A protein expression was performed by immunohistochemistry in 80 CRC tissues. The correlation between TMEM16A expression and clinicopathological parameters, and known genes and proteins involved in CRC was analyzed. The results showed that TMEM16A mRNA expression was frequently detected in 51 CRC tissues (76%), whereas TMEM16A protein expression was determined at a relatively lower frequency (26%). TMEM16A mRNA expression in tumor tissues was higher than its expression in normal para-carcinoma tissues (P < 0.05). TMEM16A mRNA expression was significantly correlated with TNM stage (p = 0.039) and status of lymph node metastasis (p = 0.047). In addition, there was a strong positive correlation between TMEM16A mRNA expression and MSH2 protein. More importantly, TMEM16A protein expression was positively associated with KRAS mutation, and negatively correlated with mutant p53 protein. Logistic regression analysis demonstrated that TMEM16A mRNA expression was an important independent predictive factor of lymph node metastasis (OR = 16.38, CI: 1.91–140.27, p = 0.01). TMEM16A mRNA and protein expression was not significantly related with patient survival. Our findings provide original evidence demonstrating TMEM16A mRNA expression can be a novel predictive marker of lymph node metastasis and TMEM16A protein expression may be an important regulator of tumor proliferation and metastasis in CRC.

The past decades have seen a growing interest in natural products. Caffeic acid phenethyl ester (CAPE), a flavonoid isolated from honeybee propolis, has shown multiple pharmacological potentials, including anti-cancer, anti-inflammatory, antioxidant, antibacterial, antifungal, and protective effects on nervous systems and multiple organs, since it was found as a potent nuclear factor κB (NF-κB) inhibitor. This review summarizes the advances in these beneficial effects of CAPE, as well as the underlying mechanisms, and proposes that CAPE offers an opportunity for developing therapeutics in multiple diseases. However, clinical trials on CAPE are necessary and encouraged to obtain certain clinically relevant conclusions.

BACKGROUND:Colon cancer (CC) is one of the most common malignant tumors, while Parkinson's disease (PD) is the second most common neurodegenerative disorder. Recent accumulating evidence indicates that these two diseases are associated with each other. Also, from the perspective of long non-coding RNAs, some well-known genes such as H19 and PVT1 can link these two diseases together. Several studies have shown that patients with PD had a decreased risk of developing CC compared with patients without PD. However, controversies surround the relationship between PD and CC, and to date, no concordant conclusion has been drawn. METHODS:In this study, we aimed to assess the association between these two diseases based on lncRNA-to-lncRNA interactions. Motivated by the weighted gene co-expression network analysis method, a customized procedure was proposed and used to identify differentially correlated edges (DCEs) in the respective interaction networks for PD and CC and explore how these two diseases are linked. RESULTS:Of the two sets of DCEs for PD and CC, 16 pairs overlapped. Among them, 15 edges had opposite signs, with positive signs for CC indicating a gain of connectivity, whereas negative signs for PD indicating a loss of connectivity. CONCLUSIONS:By using the lncRNA expression profiles, and a customized procedure, an answer to the question about how PD and CC are associated is provided.

Liver InternationalVolume 41, Issue 1 p. 241-242 LETTER TO THE EDITOR The potential missing links between hepatoprotective dioscin and alcoholic liver disease Xin Liu, Xin Liu orcid.org/0000-0001-9743-3934 Eye Center, The Second Hospital of Jilin University, Changchun, ChinaSearch for more papers by this authorZhiming Ma, Corresponding Author Zhiming Ma mazhiming@jlu.edu.cn orcid.org/0000-0002-7560-9043 Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, Changchun, China Correspondence Zhiming Ma, Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: mazhiming@jlu.edu.cn Longfei Yang, Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: yanglongfei@jlu.edu.cnSearch for more papers by this authorLongfei Yang, Corresponding Author Longfei Yang mazhiming@jlu.edu.cn yanglongfei@jlu.edu.cn orcid.org/0000-0003-4153-9670 Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun, China Correspondence Zhiming Ma, Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: mazhiming@jlu.edu.cn Longfei Yang, Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: yanglongfei@jlu.edu.cnSearch for more papers by this author Xin Liu, Xin Liu orcid.org/0000-0001-9743-3934 Eye Center, The Second Hospital of Jilin University, Changchun, ChinaSearch for more papers by this authorZhiming Ma, Corresponding Author Zhiming Ma mazhiming@jlu.edu.cn orcid.org/0000-0002-7560-9043 Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, Changchun, China Correspondence Zhiming Ma, Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: mazhiming@jlu.edu.cn Longfei Yang, Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: yanglongfei@jlu.edu.cnSearch for more papers by this authorLongfei Yang, Corresponding Author Longfei Yang mazhiming@jlu.edu.cn yanglongfei@jlu.edu.cn orcid.org/0000-0003-4153-9670 Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun, China Correspondence Zhiming Ma, Department of Gastrointestinal Nutrition and Hernia Surgery, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: mazhiming@jlu.edu.cn Longfei Yang, Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, #265, Ziqiang Street, Nanguan District, Changchun, 140041, China. Email: yanglongfei@jlu.edu.cnSearch for more papers by this author First published: 18 September 2020 https://doi.org/10.1111/liv.14665Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume41, Issue1January 2021Pages 241-242 RelatedInformation

There is an almost unlimited interest in searching and developing new drugs, especially when we are in an era that are witnessing more and more emerging pathogens. Natural products from traditional medicines represent a large library for searching lead compounds with novel bioactivities. Sodium houttuyfonate is such one bioactive compound derived from Houttuynia cordata Thunb which has been employed in traditional medicine for treating infectious and inflammatory diseases. Sodium houttuyfonate has demonstrated multiple kinds of pharmacological effects, including antifungal, antibacterial, anti-inflammatory, and cardiovascular protective activities, which are discussed here to provide insights into our understanding of the pharmacological effects of SH and the underlying mechanisms.

Introduction: Gastric cancer (GC) is a cancer that develops from the lining of the stomach. Parkinson’s disease (PD) is a long-term degenerative disorder of the central nervous system that mainly affects the motor system. Even though these two diseases seem to be distinct from each other, increasing evidences suggest that they might be linked. The objective of this study is two-fold. One is to explore if these two diseases are associated from the perspective of gene expression profiles. The other is to examine if race plays an interaction role on the association between these two diseases. Methods: Moderated t-tests or moderated paired t-tests were carried out to identify differentially expressed genes (DEGs) between the diseased people and the normal controls. Upon the overlapped DEGs between PD and GC, biological relevance was investigated using GeneCards database. Results and Discussion: Our analysis results showed that PD and GC are related to each other. Furthermore, our analysis suggest that these two diseases may be associated negatively with each other, and race is unlikely to play an interaction role on this association. Conclusions: This study may serve as a pilot study, and it will stimulate more work to investigate the link between distinct diseases using big omics data.

背景:低位前切除术后临时性回肠造口关闭的最佳时机仍然是未能明确的. 目的:本研究旨在对早期(2周)和晚期(12周)造口关闭进行比较. 设计:平行、多中心、随机、对照临床研究. 设置:该研究在3家瑞士医院开展. 患者:因癌症而接受低位前切除术和临时性回肠造口术的患者. 干预:患者被随机分配至早期关闭组或晚期关闭组.在造口关闭之前,对结肠吻合的完整性进行检查. 主要结局指标:主要研究终点是切除术后6周的生活质量(胃肠道生活质量指数).次要研究终点包括安全性、可行性和低位前切除术后4个月的生活质量.