The pathogenesis of obstructive sleep apnea hypopnea syndrome (OSAHS) is multifactorial and has garnered increasing attention due to its significant societal impact and long-term health consequences. Investigation into the underlying mechanisms of OSAHS remains critical to advancing public health. The genioglossus (GG), recognized as a key muscle in maintaining upper airway patency, exhibits structural and functional changes that correlate with OSAHS severity. As such, therapeutic strategies targeting genioglossus dysfunction have become a focal point in OSAHS management. Human umbilical cord mesenchymal stem cells (hUCMSCs), characterized by their capacity for self-renewal and multipotent differentiation, demonstrate promising regenerative potential. These cells are readily obtainable, and can be efficiently isolated, cultured, expanded, and purified. Their low immunogenicity enhances their suitability for allogeneic transplantation. Given these properties, investigation into the potential protective effects of hUCMSCs on genioglossus injury in individuals with OSAHS holds considerable therapeutic promise.

BACKGROUND:Serpin peptidase inhibitor clade H member 1 (SERPINH1) is implicated in collagen processing and tumor progression, yet its role in laryngeal squamous cell carcinoma (LSCC) remains unclear. This study aimed to elucidate the clinical significance and molecular mechanism of SERPINH1 in LSCC. METHODS:Multi-cohort bioinformatics analysis (TCGA, GEO) identified SERPINH1 as a prognostic marker. SERPINH1 expression was validated in LSCC tissues (IHC, immunofluorescence, Western blot). Functional assays (CCK-8, EdU, Transwell) and xenograft models assessed malignant behaviors. Transcriptomics and co-IP/LC-MS revealed downstream pathways and interactors. Wnt agonist (SKL2001) rescue experiments confirmed pathway dependency. RESULTS:SERPINH1 was overexpressed in LSCC tissues versus adjacent normal and predicted poor survival. SERPINH1 knockdown suppressed proliferation, migration/invasion, and tumor growth in vitro and in vivo. Mechanistically, SERPINH1 bound COL7A1 to stabilize the Wnt/β-catenin signaling complex, reducing β-catenin phosphorylation and enhancing nuclear translocation. Wnt activation via SKL2001 rescued SERPINH1-knockdown phenotypes. CONCLUSION:SERPINH1 drives LSCC progression via COL7A1-mediated Wnt/β-catenin signaling activation. Targeting this axis may offer novel therapeutic strategies for LSCC.

目的:观察小儿化脓性中耳炎实施医护一体化护理模式的效果分析.方法:选择2020年3月至2021年5月我院收治的64例中耳炎患儿为实验研究对象,随机分为对照组和观察组,每组32例,对照组患儿实施常规护理模式,观察组患儿实施医护一体化模式,对比两组患儿并发症发生率.结果:观察组患者出现恶心、头痛、耳痛、耳内瘙痒等并发症的发生率明显低于对照组,差异有统计学意义(P<0.05).结论:针对小儿化脓性中耳实施医护一体化护理方式,可显著降低患儿并发症发生率,强化治疗效果,值得推广.

Many kinds of cancer cells are intrinsically sensitive to ferroptosis, and research interest regarding ferroptosis has been sparked by its significant role in many detrimental diseases. Ferroptosis is a novel type of iron-dependent cell death mediated by accumulation of reactive oxygen species and lipid peroxidation. Furthermore, a large number of small agents can induce ferroptosis in numerous kinds of cancer cells, including prostate cancer, pancreatic cancer, breast cancer, lymphomas, and renal cancer. These insights may help discover novel approaches for cancer therapeutic strategies; however, there is considerable uncertainty regarding ferroptosis in head and neck cancer (HNC). So far, no review of the current studies on this topic has been published. Therefore, we here elaborate the mechanisms of ferroptosis and summarize the latest findings regarding its role in HNC according to current literature. The respective findings shed light on the role of ferroptosis in HNC treatment with a number of important implications for future practice in HNC management, as outlined in this review.

Background Ovarian cancer represents one of the most frequent gynecological cancers and is significant cause of death for women around the world. Long non-coding RNAs (lncRNAs) are recognized as critical governors of gene expression during carcinogenesis, but their effects on the occurrence and development of ovarian cancer require further investigation. In this report, we characterized LINC00494 as a novel oncogenic lncRNA in ovarian cancer. Methods Bioinformatics analysis predicted potential interactions among LINC00494, NFκB1, and FBXO32 in ovarian cancer, which were tested by dual-luciferase reporter assay, RNA pull-down, RIP, and ChIP assay. Cancer cells were transfected with relevant treated plasmids, followed by scratch and Transwell assays. The treated cells were injected into nude mice to establish a xenograft model for testing effects of LINC00494 and its target gene in vivo. Results LINC00494 and NFκB1 were highly expressed whereas FBXO32 had low expression in ovarian cancer cells and tissues. LINC00494 was found to bind NFκB1 and increase its activity, while NFκB1 was enriched at the FBXO32 promoter region, where it acted to reduce FBXO32 transcription. Overexpression of LINC00494 elevated NFκB1 expression and enhanced cell migration, invasion and tumorigenesis, but additional overexpression of FBXO32 interfered with the tumorgenicity of ovarian cancer cells in vitro and in vivo. Conclusion Our work demonstrated that LINC00494 promoted ovarian cancer progression by modulating FBXO32 via binding with the transcription factor NFκB1. These results provided new insight into the mechanism of ovarian cancer pathogenesis and suggested new therapeutic targets.

Vanillin, the main constituents of vanillin beans, has been reported to exhibit anti-inflammatory effects. However, the effects of vanillin on the cadmium-induced lung injury are still unclear. Therefore, we assay whether vanillin has potential preventive activity on cadmium-induced lung injury in mice. Mice were given vanillin (5, 10, 20 mg/kg) and treated with cadmium for 7 days. The detection data of vanillin on lung tissue changes were analyzed after the cadmium treatment. The results displayed that vanillin obviously decreased the lung histological alterations and myeloperoxidase (MPO) activity. Vanillin also suppressed the levels of TNF-α, IL-1β, and IL-6 in BALF. Furthermore, vanillin prevented cadmium-induced NF-κB activation and upregulation the expression of tight junction protein ZO-1 and occludin. In addition, vanillin significantly increased the expression of aryl hydrocarbon receptor (AhR), and inhibition of AhR by its agonist could reverse the protective effects of vanillin on cadmium-induced lung injury. To sum up, vanillin could be a potential drug for the treatment of cadmium-induced lung injury.

全面改善黑土地耕地保护质量,充分释放黑土地保护社会生态效益是夯实东北地区粮食生产能力与推动农业绿色发展的内在要求.社会资本作为农户嵌入社会结构的重要资源,在农户黑土地保护意愿形成过程中发挥着关键性作用.基于社会资本理论,以黑龙江省绥化市为对象,利用农户调查数据,采用双栏模型,分析农户黑土地保护意愿水平,探讨社会资本对农户黑土地保护参与意愿和支付意愿的影响机理.研究表明,大多数农户在有黑土地保护参与意愿的基础上,其支付意愿水平为1181.40～1859.85元/(hm2·a).社会网络和社会参与正向激励着农户黑土地保护参与意愿,强关系网络、社会参与强度和社会参与广度对农户黑土地保护支付意愿有显著促进作用;社会信任中的一般信任正向影响农户黑土地保护参与意愿;尽管特殊信任抑制了农户黑土地保护参与意愿,却正向激励着农户黑土地保护支付意愿.因此,政府要夯实农户社会资本水平、搭建农村社区内部信息交流平台、完善黑土地生态补偿制度.

Objectives MicroRNAs regulates varieties of molecular pathways and involve in breast carcinogenesis. Here both breast cancer cell lines and human breast cancer tissues were used to investigate the roles of miR-328-3p in breast cancer. Methods The impact of miR-328-3p on proliferation of MDA-MB-231 and T47D cells was determined by MTT assay. transwell migration and matrigel invasion assays were performed to evaluate effects of miR-328-3p on migration and invasion of breast cancer cells. Caspase 3/7 activities were measured to examine the impact of miR-328-3p on radiotherapy-induced apoptosis in breast cancer cells. The possible binding site of miR-328-3p was verified by dual-luciferase reporter assay. Quantitative real-time polymerase chain reaction was performed to detect miR-328-3p expression level in breast cancer tissues. Western blot and immunohistochemical studies were used to examine protein expression in breast cancer cells and breast cancer tissue, respectively. Results miR-328-3p involved growth, migration and invasion in breast cancer cells and was associated with radiotherapy sensitivity. MiR-328-3p enhanced radiation-induced apoptosis in breast cancer cells by regulating BAX and Bcl-2 expression. Meanwhile, aberrant expression of miR-328-3p was associated with altered expression of PTEN and p-AKT in breast cancer cells. Further study showed miR-328-3p bound to 3’-UTR of PTEN. In addition, breast cancer tissues showed higher level of miR-328-3p than normal breast tissue and higher level of miR-328-3p was seen in lower stage in breast cancer. Conclusions miR-328-3p displayed essential functions in breast carcinogenesis and might be used to predict radiotherapy response and prognosis in breast cancer.

Retraction: "microRNA-539 functions as a tumor suppressor in papillary thyroid carcinoma via the transforming growth factor beta 1/Smads signaling pathway by targeting secretory leukocyte protease inhibitor," by Cheng-Bi Xu, Xue-Shi-Bo-Jie Liu, Jin-Qiu Li, Xue Zhao, Ding Xin, Dan Yu, J Cell Biochem. 2019; 10830-10846: The above article, published online on 31 January 2019 in Wiley Online Library (), has been retracted by agreement between the journal's Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. As several flaws and inconsistencies between results presented and experimental methods described were found, the editors consider the conclusions of this article to be invalid.

Aberrant expression of LINC00520 has been identified in head and neck squamous carcinoma (HNSCC). However, its function in the radiosensitivity of HNSCC remain unclear. Herein, we aimed to define the role LINC00520 in the radiosensitivity of HNSCC and identify the underlying mechanism. Tumour tissues and adjacent normal tissue were collected from HNSCC patients. Differentially expressed genes (DEGs) in HNSCC tumour were obtained from the cancer genome atlas (TCGA) database. Interactions between LINC00520 and miR-195, homeobox A10 (HOXA10) and miR-195 were evaluated by dual-luciferase reporter gene assay, RNA Immunoprecipitation (RIP), and RNA pull-down assay. The effects of LINC00520/miR-195/HOXA10 on radiosensitivity of HNSCC were analysed in the evaluation of radiotherapy outcome. Cell proliferation, invasion, migration, and apoptosis of HNSCC cells were accessedviagain- and loss-of-function approaches. Tumour xenograft in nude mice was conducted in order to confirm the resultsin vivo. LINC00520 was upregulated while miR-195 was downregulated in HNSCC cells and tissues. Silencing LINC00520 or overexpressing miR-195 promoted radiosensitivity and inhibited cell proliferation, invasion, migration, and apoptosis in HNSCC. Moreover, thesein vitrofindings were reproducedin vivoin human HNSCC xenograft in nude mice. LINC00520/miR-195/HOXA10 is involved in the radiosensitivity mediation, providing potential therapeutic target for HNSCC treatment.

目的:评估完全腹腔镜胃腔内胃手术治疗胃黏膜下肿瘤的可行性、安全性及临床应用价值.方法:回顾分析2017年9月至2018年12月为15例胃黏膜下肿瘤患者行完全腹腔镜胃腔内手术切除术的临床资料.结果:15例手术均顺利完成,术中术野稳定、清晰,无一例需要内镜干预,术后病理报告示切缘阴性.中位手术时间64(48～92)min,中位失血量25(5～80)mL,术后中位住院时间4(2～5)d.术后1例患者发现经胃管出血,经对症治疗后治愈.术后随访12个月,无局部复发病例,无一例发生胃出血、胃瘘、胃穿孔等相关并发症.结论:利用专用腹腔镜装置施行完全腹腔镜胃内手术安全、可行,经济实惠,临床应用价值较高.

喉肿物在耳鼻喉科属于发病率极高的常见性疾病,其病因尚未明了,不排除与致癌多种因素协同作用的结果有关.临床多见于呼吸困难、声音嘶哑及吞咽困难等症状,通常以手术治疗为主,传统手术技术因存在很多局限性,视觉系统不完善,术后极易造成多种不良反应.吻合器最早用于胃肠手术,经国外学者将切割吻合器应用在全喉切除术,获得良好的效果.

2019年末,我国湖北省武汉市暴发了新型冠状病毒肺炎(COVID-19)并在短时间内迅速波及全国.COVID-19患者病情变化迅速,常累及多个器官,需要多学科协作进行综合性诊疗.虽然患者最常见的临床表现为发热、乏力及干咳,但临床数据分析显示有部分患者可以鼻塞、咽痛、嗅觉障碍等耳鼻咽喉科相关症状就诊.同时在标准诊疗方案中也涉及到耳鼻咽喉科的相关内容,故充分认识耳鼻咽喉科与COVID-19诊治的相关性,有助于提高对该疾病的认识与掌握.本文从耳鼻咽喉科角度出发,对二者之间的相关性作一综述,为COVID-19诊断及治疗过程中的耳鼻咽喉科相关问题提供参考.

: NUT carcinoma (NC) is a rare, highly invasive and fatal tumor and often misdiagnosed. It typically arises from the mediastinum and midline organs and has complicated pathogenesis and poor outcome. Genetically, its pathogenesis is related to a chromosomal rearrangement involving the NUTM1 gene. In most cases, the main oncoprotein is BRD4-NUT with a translocation between NUTM1 and BRD4 genes, but in a few cases, the oncoprotein is BRD3-NUT, or NSD3-NUT. Studies have shown that the histone hyperacetylation and BRD4 hyperphosphorylation may lead to the activation of cancer circuits. Abnormal production of microRNA, inactivation of tumor suppressor genes and abnormal activation of several signaling pathways are proposed as potential mechanisms underlying the pathogenesis of NC. Currently, there is no consensus on its standard treatment for NC. Extent of surgical resection with negative margins, initial radiotherapy and part of chemotherapy regimens may significantly associated with the improvement of progression-free survival (PFS) rate and overall survival (OS) rate. Some bromodomain and extraterminal inhibitors (BETis) have shown encouraging results in the clinical trials on NC, but delayed drug resistance is still an important issue that needs to be resolved. Histone deacetylase inhibitors are also found to possess the potential in the treatment of NC. Herein, we summarize recent advances in the pathogenesis and treatment of NC.

目的:探讨双通道法应用于早期胃癌近端胃切除术中的临床价值及疗效.方法:回顾分析2015年1月至2016年12月接受腹腔镜胃癌D2根治术的41例胃癌患者的临床资料,其中19例于近端胃切除术中采用双通道法行消化道重建(双通道组),22例于全胃切除术中采用空肠食管吻合法行消化道重建(传统组).对比分析两组手术时间、消化道重建时间、出血量、术后并发症、术后进食时间及住院时间.结果:两组均顺利完成手术,无一例中转开腹,双通道组消化道重建吻合时间[(33.76±6.95)min vs.(34.5±5.12)min]、术中出血量[(91.59±19.28)mL vs.(93.40±21.14)mL]、淋巴结清扫数量[(28.00±5.21)枚vs.(27.40±4.47)枚]、术后排气时间[(4.24±1.75)d vs.(4.25±2.10)d]及术后住院时间[(12.53±3.28)d vs.(13.60±3.62)d]与传统组相比差异均无统计学意义;两组术后并发症发生率[5.3％(1/19)vs.31.8％(7/22)]差异有统计学意义(P<0.05).术后平均随访(23±11)个月,双通道组出现肠梗阻1例(5.3％),无远期并发症发生;传统组出现反流性食管炎2例(9.1％),营养不良5例(22.7％);两组均无肿瘤复发及死亡病例.结论:近端胃切除术中采用双通道吻合法较空肠食管(Roux-en-Y)吻合法更具优势,值得进一步推广应用.

模式介绍 护士为主导的多学科协作食管发音培训小组由5名护士、3名医生、1名技师组成.护士负责筛查、制定培训计划、培训、随访工作;医生负责病情评估、选择发音方式、制定培训计划;技师负责制定培训计划、语调培训.小组成员应用"食管发音筛查表"对患者进行筛查,对符合要求的患者制定培训计划,通过嗓音治疗、发音培训及跟踪辅导等方法,最终采用经国家统一认证的"食管发音评定表"对患者发音进行评定.

The first step in the development of biochips is to prepare biochip carriers. In this process, the surface of the carrier needs to be chemically treated to enable rapid and efficient immobilisation of the various probes. The quality of the carrier directly affects the performance of the microarray and is critical to the preparation of the chip. In this paper, the surface-smoothed slides were used to assemble nanofilms with large specific surface area and super-adsorption properties on the slides, and then functionalised surface modification, giving full play to the dual advantages of physical adsorption and chemical coupling. The glass cuboid is used as the chip substrate, and the cylindrical glass capillary is used as the microfluidic channel. By simply combining it with the flow pump, a three-dimensional microfluidic immunoassay device is constructed. By comparing the properties of protein immobilisation, immune response efficiency and signal-to-noise ratio in three-dimensional devices, it is compared with traditional two-dimensional microfluidic chips. The scores in the experimental group and the control group were significantly lower than those in the initial diagnosis, P < 0.001. The symptom scores of the experimental group and the control group were P > 0.04, indicating that there was no statistical difference between the two groups. The use of topical drugs to treat chronic localisation problems can effectively alleviate the patient's discomfort and reduce the symptoms of skin lesions. At the same time, it has high safety and good promotion value.

Papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy, with growing incidence every year. microRNAs (miRs) are known to regulate the physiological and pathological processes of cancers, such as proliferation, migration, invasion, survival, and epithelial-mesenchymal transition (EMT). Herein, this study aimed to investigate the effect of miR-539 on cell proliferation, apoptosis, and EMT by targeting secretory leukocyte protease inhibitor (SLPI) via the transforming growth factor β1 (TGF-β1)/Smads signaling pathway in PTC. First, PTC-related differentially expressed genes and regulatory miR were screened using bioinformatics analysis, dual luciferase reporter gene assay, and ribonucleoprotein immunoprecipitation, which identified the SLPI gene and the regulatory miR-539 for this study. We identified SLPI as a highly expressed gene in PTC tissues, and SLPI was targeted and negatively regulated by miR-539. Then, we introduced a series of miR-539 mimics, miR-539 inhibitors, and small interfering RNA against SLPI plasmids into CGTHW-3 cells to examine the effects of miR-539 and SLPI on the expression of TGF-β1/Smads signaling pathway-, EMT-, and apoptosis-related factors, as well as cell proliferation, migration, invasion, and apoptosis. The obtained results indicated that CGTHW-3 cells treated with silenced SLPI or overexpressed miR-539 suppressed the cell proliferation, migration, invasion abilities, and resistance to apoptosis of PTC cells, corresponding to increased expression of Bcl-2-associated X protein, TGF-β1, Sekelsky mothers against dpp 4, and epithelial cadherin, and decreased B cell lymphoma 2, Vimentin, and N-cadherin. Altogether, we concluded that overexpressed miR-539 could inhibit the PTC cell proliferation and promote apoptosis and EMT by targeting SPLI via activation of the TGF-β1/Smads signaling pathway.

胃癌在我国占肿瘤总死亡率的第二位[1],主要依靠胃镜下对可疑肿瘤部位取活检后做病理来确诊。肿瘤标志物（CEA,CA125等）对癌症患者的筛查具有预测作用[2],但敏感性和特异性较低。近年来随着非编码RNA的发现,其在癌症组织和健康组织中存在差异性表达成为研究热点,并有望成为肿瘤的新的血清学标志物及治疗的靶点[3]。HOTAIR作为一种长链非编码RNA已经被证实在胃

喉全切除术后食管发音康复训练方法包括食管基音形成、食管音基本功练习、食管音与语言配合和食管语言完成四个阶段,要根据患者康复前和康复过程中出现的心理问题给予针对性心理干预,解除患者心理负担,使其以良好心态积极配合食管发音康复训练,尽快重新建立第二语言功能,方能提高生存质量,回归正常的工作学习和生活.