ABSTRACTHosta (Hosta plantaginea L.) is a perennial herbaceous plant belonging to the family Liliaceae. It is widely cultivated for its decorative foliage and dainty, colourful flowers. Fusarium is a globally prevalent plant pathogen. During 2020–2021, 116 Fusarium isolates were isolated from 300 hosta leaf blight samples collected from eight cities in China, and 65 representative isolates we selected for further study. The 65 isolates were identified based on the concatenated sequences of the tef1 and rpb2 genes, which grouped them into 12 Fusarium species, including F. acuminatum (27.59%), F. verticillioides (15.52%), F. oxysporum (10.34%), F. armeniacum (10.34%), F. ipomoeae (8.62%), F. proliferatum (6.9%), F. subglutinans (6.9%), F. humuli (3.45%), F. petersiae (3.45%), F. brachygibbosum (3.45%), F. graminearum (1.72%), and F. compactum (1.72%). Pathogenicity tests demonstrated that all Fusarium isolates exhibited high pathogenicity towards hosta leaves. Whereas in F. proliferatum isolates were found to be the most pathogenic. All Fusarium species obtained in this study are first reported as the causal agents of hosta leaf blight in China except F. oxysporum and F. ipomoeae. The current research offers insights into disease management, phylogenetic relationships amongst Fusarium species and the diversity of Fusarium species associated with hosta leaf blight.

Haploid androgenetic stem cells (haSCs) are revolutionary resources for studying animal traits and greatly impact livestock breeding, but livestock haSCs have yet to be obtained. Here, we report the derivation of cattle and sheep haSCs using a novel FACE medium, and these cells harbor formative pluripotency features. Notably, knockout cattle and sheep can be efficiently obtained upon injection of the protaminized haSCs with targeted gene mutations into oocytes. ### Competing Interest Statement The authors have declared no competing interest. All WGBS, CUT&Tag, buRNA-Seq, scRNA-seq, WGS data are available through GEO under the accession numbers GSE250491, GSE250493, GSE250494, GSE250495, and GSE250496. In addition, the SuperSeries GSE250497 links all the above data sets together.

Sweet cherry (Prunus avium L.) is commonly affected by fruit rot, leading to reduction in yield and economic loss. In 2022, 144 symptomatic cherry fruit were randomly collected from 11 orchards in Yantai city. A total of 107 fungal strains were isolated and classified into eight species based on morphological and multi-locus phylogenetic analysis, including Alternaria alternata/tenuissima species complex (79.44%), Fusarium lateritium species complex (FLSC) (9.34%), F. verticillioides (2.80%), F. incarnatum (2.80%), F. proliferatum (1.87%), Diaporthe eres (1.87%), Botryosphaeria dothidea (0.94%), and Nothophoma quercina (0.94%), wherein A. alternata/tenuissima species complex was the most dominant genus. All fungal isolates showed aggressiveness on both wounded and unwounded cherry fruit, wherein D. eres exhibited the strongest virulence, and F. verticillioides had the lowest pathogenicity. This work systematically studied the fungal pathogens related to cherry fruit rot and first reported D. eres, B. dothidea, F. verticillioides, F. incarnatum, and N. quercina as the causal agents associated with cherry fruit rot in China. The results of this study will be helpful to get a better understanding about the causal agents of cherry fruit rot in China and provide a theoretical basis for the field control of this disease.

利用脂多糖(LPS)诱导奶牛乳腺上皮细胞(Bovine mammary epithelial cells,BMECs)建立炎症细胞模型,采用RT-qPCR检测BMECs中炎症因子及AKT1 mRNA表达变化;采用BSP(Bisulfite sequencing PCR)检测AKT1基因启动子区域甲基化水平;采用组织细胞甘油三酯(Triglyceride,TG)含量酶法测定试剂盒检测细胞中甘油三酯含量;采用Bovine lactose ELISA KIT检测细胞中乳糖含量;采用Western blot技术检测β-casein、AKT1、P-AKT1、DNA甲基转移酶DNMT1和DNMT3A蛋白表达情况.试验结果表明,LPS诱导后奶牛乳腺上皮细胞中甘油三酯、乳糖和β-酪蛋白合成减少,AKT1表达量减少,P-AKT1蛋白表达量也减少,同时DNMT1和DNMT3A蛋白表达量增加,AKT1基因启动子甲基化水平升高,表明DNA甲基化参与乳腺炎条件下奶牛泌乳调控,LPS也通过促进DNA甲基转移酶表达提高AKT1基因甲基化水平而抑制AKT1表达,抑制乳脂、乳糖和乳蛋白合成.

Mastitis is among the main factors affecting milk quality and yield. Although DNA methylation is associated with mastitis, its role in mastitis remains unclear. In this study, a bovine mastitis mammary epithelial cells (BMMECs) model was established via Staphylococcus aureus infection of bovine mammary gland epithelial cells (BMECs). Bisulfite sequencing PCR was used to determine the methylation status of the AKT1 promoter in BMMECs. We found that the degree of the AKT1 promoter methylation in BMMECs was significantly greater than that in BMECs, and the expression levels of genes related to milk protein synthesis were significantly decreased. We used the pdCas9-C-Tet1-SgRNA 2.0 system to regulate the methylation status of the AKT1 promoter. High-efficiency sgRNAs were screened and dCas9-guided AKT1 promoter demethylation vectors were constructed. Following transfection with the vectors, the degree of methylation of the AKT1 promoter was significantly reduced in BMMECs, while AKT1 protein levels increased. When the methylation level of the AKT1 promoter decreased, the synthesis of milk proteins and the expression levels of genes related to milk protein synthesis increased significantly. The viability of the BMMECs was enhanced. Taken together, these results indicate that demethylation guided by the pdCas9-C-Tet1-SgRNA 2.0 system on the AKT1 promoter can reactivate the expression of AKT1 and AKT1/mTOR signaling pathway-related proteins by reducing the AKT1 promoter methylation level and promoting the recovery milk protein expression in BMMECs, thereby alleviating the symptoms of mastitis.

Maize leaf spot occurs worldwide and affects maize production. Maize can be infected by several pathogens causing leaf spot, such as Bipolaris zeicola, Bipolaris maydis, Curvularia species, Alternaria species, etc. In the current study, 30 Epicoccum isolates recovered from symptomatic maize leaves were identified based on morphological characteristics, pathogenicity, and multilocus sequence analyses of nuLSU, ITS, tub2, and rpb2. These maize isolates were grouped into five Epicoccum species, including E. nigrum, E. layuense, E. sorghinum, E. latusicollum, and E. pneumoniae. Pathogenicity tests showed that all five Epicoccum species could produce small ellipse- and spindle-shaped spots on maize leaves. The lesion center was grayish yellow to dark gray and surrounded by a chlorotic area. Furthermore, the Epicoccum isolates exhibited high pathogenicity to 20 main maize varieties of Heilongjiang Province but showed different sensitivities to the commonly used fungicides carbendazim and tebuconazole. In addition, these Epicoccum isolates showed different production capacity of pectinase, cellulase, protease, amylase, laccase, and gelatinase, but all showed high lipase activity. This is the first report globally of E. layuense, E. latusicollum, and E. pneumoniae as causal agents of maize leaf spot. E. pneumoniae was first reported as a plant pathogen.

Panicle Hydrangea (Hydrangea paniculate) is an ornamental flowering plant native to China and Japan. In August 2019, leaf spot symptoms with about 30% disease incidence were observed on panicle hydrangea in two grower fields (about 0.1 ha in total) of Northeast Agriculture University, China (126.72°E, 45.74°N). Symptoms initially appeared on the lower and older leaves and showed small subcircular brown spots with dark-brown edges on both sides. As the disease progressed, the necrotic spots enlarged, became irregular, coalesced, and the infected leaf blighted in approximately 2 weeks. Panicle hydrangea leaf samples (n=15) from different plants that showed spot symptoms were collected and surface sterilized with 70% ethanol for 10 s, followed by 0.5% NaClO treatment for 4 min, and rinsed in sterile water 3 times. Thereafter, leaf samples were placed on potato dextrose agar (PDA) and incubated at 25°C for 7 days. Fifteen hyphal-tipped pure cultures were obtained. Colonies growing on PDA for 7 days were olive green to dark green, exhibited a velvet-like texture and sometimes were radially furrowed and wrinkled. Margins varied from white gray to dark green without prominent exudates. The back of the plate showed dark green to black. Conidiophores were up to 180 to 600 µm long, 2.8 to 4.5 µm wide (n=50), subcylindrical-filiform, straight, septate, and unbranched or rarely branched. Ramoconidia were 0 to 1 septate, cylindrical to clavate, smooth-walled, 8 to 22 μm long (n=50). Conidia were single-celled, lemon-shaped, smooth-walled and 2.0 to 5.0 µm (diameter) (n=50). To confirm the identity, three genomic DNA regions, internal transcribed spacer (ITS), partial translation elongation factor-1 alpha (EF), and actin (ACT) of the representative isolate BAI-1 were amplified with primer pairs ITS1/4, EF1-728F/986R, and ACT-512F/783R, respectively (Bensch et al. 2012; Jo et al. 2018). DNA sequences of the isolate from ITS, EF, and ACT showed 99.81% (514/515 bp), 99.10% (219/221 bp), and 99.54% (216/217 bp) nucleotide identity with those of C. tenuissimum CBS 125995, respectively (GenBank accession nos. HM148197, HM148442, and HM148687). The sequences of isolate BAI-1 were deposited in GenBank (accession nos. MW045455, MW052465, and MW052466). To fulfill Koch's postulates, five healthy 2-year-old panicle hydrangea plants grown in pots were surface sterilized with 70% ethanol, washed twice with sterile distilled water, and sprayed with a conidial suspension of strain BAI-1 (adjusted to 1×106 conidia/ml using a hemocytometer), maintained in a greenhouse at 25°C and 85% relative humidity. Five plants sprayed with sterilized water served as controls. The inoculated plants showed leaf spot symptoms that were similar to those previously observed in the fields after 7 days, whereas control leaves remained healthy. The fungus was reisolated from symptomatic leaves and its identity was confirmed by morphological and molecular method. These experiments were repeated twice. So far, C. tenuissimum was reported to cause leaf spot of alfalfa (Han et al. 2019) and castor (Liu et al. 2019). To our knowledge, this is the first report of leaf spot disease in panicle hydrangea caused by C. tenuissimum in China. Leaf spot has a negative effect on the aesthetic value of panicle hydrangea, and this report will assist with monitoring distribution of the disease as well as developing management recommendations.

MicroRNA是一种由20～24个核苷酸组成的短链非编码RNA,可以通过与靶基因3'UTR结合调控基因表达,但miR-214在奶牛泌乳过程中的研究还很少.本研究应用奶牛乳腺上皮细胞模型,通过过表达miR-214,检测奶牛乳腺上皮细胞中乳糖、乳脂和乳蛋白的合成情况.结果表明,在奶牛乳腺上皮细胞内转染miR-214后,miR-214的表达显著上升,而乳糖、乳脂和乳蛋白的含量均显著下降,说明miR-214能够抑制乳糖、乳脂和乳蛋白的合成,影响奶牛乳腺上皮细胞的泌乳功能.

Maize (Zea mays L.) is the most important crop in Heilongjiang province. In July 2021, maize stalk rot was observed on approximately 10% of maize in a 2.4 ha field of Xiangfang District, Harbin City (N45°44'23″, E126°43'19″). Infected plants showed softening of the stalks at the lower internodes, and the pith tissue was disintegrated and brown to reddish. Fifteen symptomatic plants were collected from the field. The discolored stalk pith tissues were cut into small pieces (4 × 2 mm), superficially disinfected with 1% NaClO for 3 min, 70% ethanol for 10 s, and then washed three times with sterile distilled water. The disinfected tissues were placed on potato dextrose agar (PDA) amended with streptomycin sulfate (50 mg/L) and incubated at 25°C for 1 week. Twenty-one cultures were obtained using hyphal tip technology and cultured on PDA for 7 days at 25°C for morphological and molecular analyses. The mycelia of the cultures were initially white but became grayish with time, and reddish-brown diffusible pigments were produced. A dark green discoloration was produced on malt extract agar (MEA) using the NaOH spot test (REF). Pycnidia were brown, predominantly spheroidal, and measured 80.1 to 130.2 × 110.5 to 220.6 μm. Conidia were ellipse, aseptate, and in a size range of 4.3 to 6.8 × 2.1 to 3.2 μm. The isolates were initially identified as Epicoccum latusicollum based on morphological features (Chen et al. 2017). To confirm the identity of E. latusicollum, primers TUB2Fd/TUB4Rd, LR0R/LR5, ITS1/ITS4 and RPB2-5F2/fRPB2-7cR (Valenzuela-Lopez et al. 2018) were used to amplify beta tubulin (tub2), nuclear large subunit rDNA (LSU), internal transcribed spacer (ITS), and RPB2 genes, respectively for the representative isolate JF3. These sequences were deposited in GenBank (GenBank accession no. OK490498, OK445527, OK483136, and OK490497) and had 100% (276/276 bp), 100% (842/842 bp), 100% (501/501 bp), and 100% (589/589 bp) nucleotide identity with E. latusicollum isolate GZDS2018BXT010 (GenBank accession no. MK516208, MK516207, MK516206, and MK852278). To fulfill Koch's postulates, pathogenicity tests for all isolates were performed by individually inoculating surface-disinfected stalks of five healthy maize plants (10-leaf stage) between the 2nd and 3rd stem nodes with 20 μL conidial suspension at a concentration of 106 conidia/mL as described by Zhang et al. (2016). Five other healthy surface-disinfected maize plants inoculated with sterile distilled water served as control. All plants were kept at 25 ± 0.5°C in a greenhouse with a photoperiod of 12 h and approximately 90% relative humidity. After 10 days, all inoculated plants showed symptoms that were similar to those of the infected maize plants observed in the field, whereas the control plants were asymptomatic. The Epicoccum isoaltes were re-isolated from symptomatic plants, and species identification was performed using the morphological and molecular methods described above. To our knowledge, this is the first report of E. latusicollum causing maize stalk rot in China, and this report will assist with monitoring distribution of the disease and developing management recommendations.

A novel cellulase-producing actinobacterium, designated strain NEAU-L178(T), was isolated from soil sample collected from Qiqihaer, Heilongjiang Province, PR China. A polyphasic study was carried out to determine the taxonomic status of the strain. On the basis of 16S rRNA gene sequence analysis, strain NEAU-L178T should be classified into the genus Nonomuraea and is closely related to Nonomuraea cavernae SYSU K10005(T) (99.31% 16S rRNA gene sequence similarity), Nonomuraea glycinis NEAU-BB2C19(T) (98.75%), Nonomuraea guangzhouensis NEAU-ZJ3(T) (98.75%) and `Nonomuraea rhizosphaerae' NEAU-mq18(T) (98.34 %). The digital DNA-DNA hybridization values between them are 27.1, 26.1, 42.0 and 30.9%, and the whole-genome average nucleotide identity values between them are 83.1, 82.3, 90.3 and 85.8 %, respectively. The whole-cell hydrolysates contained glucose, ribose, arabinose and madurose. The menaquinones were identified as MK-9(H 0), MK-9(H 4) and MK-9(H 2). The major fatty acids were C-16: 0, iso-C-17 :0 and C-17: 0 10-methyl. The detected polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy--phosphatidylethanolamine, phosphatidylinositol and three unidentified phospholipids. The genomic DNA G+C content was 69.7mol%. In addition, whole-genome analysis indicated that strain NEAU-L178(T) had the potential to degrade cellulose. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic data, strain NEAU-L178(T) can be differentiated from its close phylogenetic relatives and represents a novel species of the genus Nonomuraea, for which the name Nonomuraea aurantiaca sp. nov. is proposed. The type strain is NEAU-L178(T) (=JCM 34799(T)=CGMCC 4.7741(T)).

以健康的泌乳期中国荷斯坦奶牛的乳腺上皮细胞(Dairy cow mammary epithelial cells,DCMECs)为实验模型,探究miR-9-5p对奶牛乳腺上皮细胞乳脂合成的影响.通过体外转染miR-9-5p mimics的方式处理奶牛乳腺上皮细胞.随后采用MTT方法测定细胞增殖能力,采用western blotting技术检测乳脂合成相关信号通路蛋白SREBP1的表达情况,再采用甘油三脂检测试剂盒检测奶牛乳腺上皮细胞中甘油三脂含量的变化.实验结果显示miR-9-5p对奶牛乳腺上皮细胞的增殖有明显的抑制作用,同时miR-9-5p对乳脂合成相关信号通路蛋白SREBP1的表达也有明显的抑制作用,且显著抑制甘油三脂的合成,说明miR-9-5p对奶牛乳腺上皮细胞乳脂的合成有着负调控作用.本实验试图从分子水平揭示中国荷斯坦奶牛乳品质差异的原因,进一步完善中国荷斯坦奶牛泌乳的分子机制.

以健康的泌乳期中国荷斯坦奶牛的乳腺上皮细胞(DCMECs)为实验模型,构建DNMT3A过表达载体pcDNA3.1-DNMT3A,用脂质体转染奶牛乳腺上皮细胞,采用荧光定量PCR技术检测奶牛乳腺上皮细胞中DNMT3A的mRNA表达,采用Bovine milk fat ELISA KIT检测细胞中的乳脂含量,采用Bovine lactose ELISA KIT检测细胞中的乳糖含量.结果表明,过表达DNMT3A后,奶牛乳腺上皮细胞生成的乳脂和乳糖含量均减少,表明DNA甲基化水平升高能够抑制奶牛乳腺上皮细胞乳脂和乳糖的合成与分泌.

为探究miR-142-3p在MCF-7细胞中作用机理,采用RNA免疫共沉淀技术和双荧光素酶报告基因技术筛选及验证miR-142-3p作用靶基因;蛋白质免疫印迹技术验证靶基因及其介导的PI3K-AKT-mTOR信号通路蛋白表达量及通路活性;应用实时荧光定量PCR验证靶基因PTEN对miR-142-3p调节关系.结果表明,miR-142-3p靶向调节AKT和PTEN表达;miR-142-3p过表达组中PI3K-AKT-mTOR通路活性显著降低;miR-142-3p抑制组中PI3K-AKT-mTOR通路活性显著上升;抑制PTEN表达显著提高miR-142-3p表达量.因此miR-142-3p靶向调节AKT和PTEN表达继而抑制PI3K-AKT-mTOR信号通路活性,PTEN与miR-142-3p存在调节关系.

Leucine is an essential amino acid in the milk production of bovine mammary glands, but the regulatory roles and molecular mechanisms of leucine are still not known well. This study investigated the roles of leucine on milk synthesis and explored the corresponding mechanism in bovine mammary epithelial cells (BMECs). Leucine (0, 0.25, 0.5, 0.75, 1.0, and 1.25 mM) was added to BMECs that were cultured in FBS-free OPTI-MEM medium. Leucine significantly promoted milk protein and milk fat synthesis and also increased phosphorylation of mTOR signaling protein and the protein expression levels of SREBP-1c, with the most significant effects at 0.75 mM concentration. Leucine increased the expression and nuclear localization of DDX59, and loss and gain of gene function experiments further reveal that DDX59 mediates the stimulation of leucine on the mRNA expression variation of mTOR and SREBP-1c genes. PI3K inhibition experiment further detected that leucine upregulated expression of DDX59 and its downstream signaling via PI3K activation. ChIP-qPCR analysis further proved the binding of DDX59 to the promoter regions of mTOR and SREBP-1c. In summary, these data prove that DDX59 positively regulates the mTOR and SREBP-1c signaling pathways leading to synthesis of milk, and leucine regulates these two signaling pathways through the PI3K-DDX59 signaling.

Murine mammary gland is an ideal model for studying the development and milk synthesis of dairy animals. MicroRNAs play an important role in milk synthesis and mammary gland development，however, the molecular mechanism of miR-142-3p continue to be poorly understood. Here, we knocked down miR-142-3p expression in vitro and vivo increased prolactin receptor expression and activated many downstream cellular proteins, such as mammalian target of rapamycin, sterol regulatory element-binding transcription factor 1, cyclin D1 and signal transducer and activator of transcription 5. Additionally, miR-142-3p knockdown in mouse mammary gland epithelial cells increased proliferation but not viability, induced cell cycle progression, decreased apoptosis, and increased the expression of triglycerides and β-Casein. Moreover, knockdown miR-142-3p in murine mammary gland tissue in vivo affected the structure and function of the mammary gland, which showed an increased number of lobules and ducts and was more capable of producing milk. However, overexpression of miR-142-3p had the opposite effects. In summary, these data uncover that miR-142-3p regulates milk synthesis and structure of murine mammary glands via PRLR-mediated multiple signaling pathways.

为确定FGF2调节奶牛乳腺分支主要信号通路,首先将青春期奶牛乳腺类器官镶嵌在鼠尾Ⅰ型胶原中并添加FGF2作三维培养;通过FGFR1受体抑制剂(FGF2+PD173074组)、PI3K/AKT信号通路抑制剂(FGF2+LY-294002组)或MAPK/ERK信号通路抑制剂(FGF2+U0126组)阻断信号通路,确定不同处理下84h时奶牛乳腺分支率;利用Western blot检测处理时间0、15、60、240和600 min时各组p-AKT、AKT、p-ERK和ERK相对蛋白表达量.研究发现,FGF2主要通过MAPK信号通路影响奶牛乳腺分支.文章揭示FGF2在影响奶牛乳腺分支形态发生中起主要作用的下游信号通路,对奶牛乳腺分支形态发生分子机制研究具有推动作用.

Enzyme-assisted aqueous extraction processing (EAEP) is an alternative method to extract oil which is safer and more environmental-friendly.EAEP results in four fractions:free oil,oil-in-water emulsion,liquid fraction (skim),and residual fraction.The surplus amounts of residual are the limitation of EAEP.The residue contains a large amount of fiber.EAEP residue fiber based edible films containing citric acid and glycerol was prepared.In the single-factor experiment,the effect of citric acid concentration,glycerol concentration,crosslink time on the tensile strength,elongation at break,water vapor permeability of EAEP residue fiber based edible films was studied.In order to optimize the process parameters and investigate the interrelationship between the three factors and water vapor permeability,response surface methodology was employed.Results indicated that the water vapor permeability was influenced the most by glycerol concentration,crosslink time and the least by citric acid concentration.The optimum conditions were as follows:citric acid concentration of 20％,glycerol concentration of 36％ and the crosslink time of 5 min.Under the optimum conditions,the water vapor permeability of the EAEP residue based edible film was 1.81 g·m/(h·Pa·m2).The scanning electron microscopy showed that the edible film prepared under the optimum process conditions had a relatively smooth surface.The infrared spectrum analysis indicated that crosslink may have taken place between citric acid and the fiber of EAEP residue.Glycerol may affect the reaction between citric acid and the fiber.The results can provide a reference for the production of EAEP residue based edible film.

Many studies have indicated that microRNAs (miRNAs) influence the development of the mammary gland by posttranscriptionally affecting their target genes. The objective of this research was to identify novel miRNAs in the mammary gland of dairy goats with a bioinformatics approach that was based on expressed sequence tag (EST) and genome survey sequence (GSS) analyses. We applied all known major mammals, miRNAs to search against the goat EST and GSS databases for the first time to identify new miRNAs. We, then, validated these newly predicted miRNAs with stem–loop reverse transcription followed by a SYBR Green polymerase chain reaction assay. Finally, 29 mature miRNAs were identified and verified, and of these, 14 were grouped into 13 families based on seed sequence identity and 85 potential target genes of newly verified miRNAs were subsequently predicted, most of which seemed to encode the proteins participating in regulation of metabolism, signal transduction, growth and development. The predicting accuracy of the new miRNAs was 70.37%, which confirmed that the methods used in this study were efficient and reliable. Detailed analyses of the sequence characteristics of the novel miRNAs of the goat mammary gland were performed. In conclusion, these results provide a reference for further identification of miRNAs in animals without a complete genome and thus improve the understanding of miRNAs in the caprine mammary gland.

传统的核酸检测技术如放射性核素、荧光、化学修饰的探针以及核酸扩增等技术无法检测活细胞中核酸的表达量.而活细胞RNA纳米检测技术和传统的检测技术相比,利用纳米金颗粒为探针能对活细胞进行检测,实验步骤更为简单,可以在自然的、无扩增的条件下观察RNA,这可真实地反应基因表达与表型之间的关系.miRNA是一类非编码RNA,其长度为20～ 24个碱基,在生命活动中起重要的作用.本文应用活细胞RNA检测纳米技术结合荧光定量PCR分别检测正常的乳腺上皮细胞系及乳腺上皮癌细胞系中内源性miR-142-3p的表达,发现乳腺癌细胞系内源性miR-142-3p的表达显著高于正常乳腺上皮细胞系中miR-142-3p的表达,结果提示miR-142-3p可能在乳腺癌细胞发生发展中起到调控作用.