Background Accurate identification of complete responders before neoadjuvant chemoradiotherapy (nCRT) is essential for organ-preservation strategies for rectal cancer. However, current preoperative radiological assessment methods lack sufficient accuracy. The purpose of this study is to evaluate the diagnostic value of contrast-enhanced ultrasound (CEUS) in distinguishing complete response (CR) in patients with rectal cancer after nCRT. Methods In this prospective study, 100 patients with rectal cancer treated between January 2023 and February 2025 underwent endorectal ultrasound (ERUS) and CEUS examinations before and 6–8 weeks after nCRT. Quantitative perfusion parameters were derived using time–intensity curve analysis. Surgical histopathology served as the reference standard for pathological CR, and multimodal clinical evaluation defined complete CR. Diagnostic performance was assessed using logistic regression and receiver operating characteristic curve analysis. Result A total of 100 participants (mean age, 57 ± 11 years; 77 men) were included. Pathological CR occurred in 29 (29.0%) participants, and clinical CR in 13 (13.0%), yielding an overall CR rate of 42%. Post-nCRT CEUS parameters—relative area under the time–intensity curve (rAUC*), relative enhancement intensity, and enhancement intensity difference—were significant predictors of CR (all p < 0.05). The AUCs were 0.72 (95% CI: 0.617, 0.829), .76 (95% CI: 0.665, 0.858), and 0.76 (95% CI: 0.695, 0.875), respectively. Combined models achieved AUCs of 0.80 (95% CI: 0.708, 0.887) and 0.82 (95% CI: 0.741, 0.903), respectively. Conclusion Quantitative contrast-enhanced ultrasound (CEUS) parameters, including enhancement intensity (EI), relative AUC*, and their derived ratio values (rEI, rAUC*) and difference (ΔEI), demonstrated robust discriminative capacity in complete response participants after nCRT. The CEUS-based diagnostic model also demonstrated a high level of diagnostic efficacy. The clinical diagnostic model constructed from the above parameters demonstrated higher diagnostic value.

Introduction:The diagnostic accuracy of traditional imaging examination in predicting ypT stage of rectal cancer after neoadjuvant therapy is significantly reduced, which would affect patients’ subsequent treatment choices. This study aimed to investigate the use of endorectal shear wave elastography (SWE) for diagnosing ypT0 stage of rectal cancer after neoadjuvant chemoradiotherapy (nCRT).Methods:Sixty patients with rectal cancer were prospectively recruited in this study. Data on endorectal ultrasound (ERUS) and SWE parameters were collected before nCRT and 6–8 weeks after nCRT. Postoperative pathological results were the gold standard for evaluating the diagnostic accuracy of SWE and ERUS in predicting the ypT0 stage of rectal cancer after nCRT. Receiver operating characteristic (ROC) curve analysis was used to determine the cut-off values of the SWE parameters that best corresponded to the ypT0 stage and analyze the sensitivity, specificity, and accuracy.Results:The diagnostic accuracies of using ERUS to predict the ypT and ypT0 stages of rectal cancer after nCRT were 58.1% (18/31) and 64.3% (9/14), respectively. The ROC curve was constructed with the lesion’s Emean, Emean corrected (EC), Emean difference (ED), Emean corrected differencede (ECD), Emean descendding rate (EDR) and Emean corrected descendding rate (ECDR) values after nCRT, the cut-off values of diagnosing the ypT0 stage were 64.40 kPa, 55.45 kPa, 72.55 kPa, 73.75 kPa, 50.15%, and 55.93%, respectively; the area under the curve (AUC) for diagnosing the ypT0 stage was 0.924, 0.933, 0.748, 0.729, 0.857 and 0.861, respectively. The EC value showed the best diagnostic performance.Conclusion:SWE could improve the accuracy of conventional ERUS in diagnosing the ypT0 stage of rectal cancer after nCRT. It is expected to become a new method to help predict pathological complete responses in clinical practice and provide new evidence for the watch-and-wait approach.

e14583 Background: Interleukin (IL)-12 is a potent immunomodulatory cytokine demonstrating robust antitumor activity in multiple preclinical studies and potent immune-stimulating potential in humans. However, systemic delivery of IL-12 can result in life-threatening toxicity and therefore has shown limited efficacy at doses that can be safely administered. ABO2011 is a human IL-12 mRNA encapsulated in lipid nanoparticle and has been developed to be more effective and less toxic through the use of novel delivery technology and intratumoral injection. Methods: This phase I trial enrolled adults with advanced solid tumors who failed available standard treatments (NCT05392699). The trial was designed to primarily evaluate the safety of ABO2011 in single-dose cohorts and multiple-dose cohorts using “3+3” dose escalation approach. Patients (pts) would receive intratumoral ABO2011 at either a single dose, QW with 3 doses overall, every 3 weeks (Q3W) or QW followed by Q3W. Results: As of 09 December 2023, a total of 20 pts were enrolled and received at least one dose of ABO2011 at 4 μg single dose, 4 μg QW for 3 times, 12 μg single dose, 12 μg QW for 3 times followed by Q3W, 24 μg Q3W and 48 μg Q3W. Seventeen (85%) pts experienced treatment-related adverse events (TRAEs), and all were grade 1 or 2, including pyrexia (35%), pain (25%), and gamma-glutamyl transferase increased (25%). There were no dose-limiting toxicities or treatment-related SAEs reported and no TRAEs leading to treatment discontinuation. Of the 17 evaluable pts per RECISIT v1.1, 5 maintained stable disease, and the progression free survivals achieved 6.0 months, 5.6 months and 4.1 months for pts with colorectal cancer (6th line), non-small cell lung cancer (5th line) and pancreatic cancer (4th line), respectively. Peripheral blood IL-12 and IFN-γ levels increased and peaked at 24 hours and 48 hours after dosing, respectively, indicating expression of IL-12 and subsequent induction of IFN-γ after ABO2011 administration. Dose-dependent elevation of CXCL9, CXCL10, CXCL11 in peripheral blood was observed, consistent with the activation of downstream pathway of IFN-γ. CD8 T cells infiltration and PD-L1 expression were increased in tumor microenvironment (TME) at day 7, and further RNA sequencing analysis revealed increase of activated CD8 T cell and antigen processing and presentation after study treatment, suggesting the regulation of TME by ABO2011 and supporting the combination with PD-1(L1) inhibitor. Conclusions: Intratumoral ABO2011 was safe and well-tolerated in pts with advanced solid tumors. The pharmacodynamic changes were as expected. Promising preliminary antitumor activities of the single agent and the pharmacodynamic profiles support further investigation of ABO2011 in combination with immune checkpoint inhibitor. Clinical trial information: NCT05392699 .

BACKGROUND:Intestinal lymphoma is a rare tumor. Contrast-enhanced ultrasound (CEUS) findings of intestinal lymphoma have not been reported previously, and the relationship between CEUS and clinicopathological features and prognostic factors is still unknown.AIM:To describe the B-mode US and CEUS features of intestinal lymphoma and investigate the correlation of CEUS and histopathological features.METHODS:This was a single-center retrospective study. Eighteen patients with histologically confirmed intestinal lymphoma underwent B-mode US and CEUS examinations between October 2016 and November 2019. We summarized the features of B-mode US and CUES imaging of intestinal lymphoma and compared the frequency of tumor necrosis in intestinal lymphomas with reference to different pathological subtypes (aggressive or indolent) and clinical stage (early or advanced). The time-intensity curve parameters of CEUS were also compared between patients with normal and elevated serum lactate dehydrogenase.RESULTS:In B-mode imaging, four patterns were observed in intestinal lymphoma: Mass type (12/18, 66.7%), infiltration type (1/18, 5.6%), mesentery type (4/18, 22.2%) and mixed type (1/18, 5.6%). All cases were hypoechoic and no cystic areas were detected. On CEUS, most cases (17/18, 94.4%) showed arterial hyperechoic enhancement. All cases showed arterial enhancement followed by venous wash out. A relatively high rate of tumor necrosis (11/18, 61.1%) was observed in this study. Tumor necrosis on CEUS was more frequent in aggressive subtypes (10/13, 76.9%) than in indolent subtypes (1/5, 20.0%) (P = 0.047). There were no correlations between tumor necrosis and lesion size and Ann Arbor stage. There was no significant difference in time-intensity curve parameters between normal and elevated lactate dehydrogenase groups.CONCLUSION:B-mode US and CEUS findings of intestinal lymphoma are characteristic. We observed a high rate of tumor necrosis, which appeared more frequently in aggressive pathological subtypes of intestinal lymphoma.

目的 探讨经直肠腔内超声弹性成像对局部进展期直肠癌新辅助放化疗后T分期的应用价值.方法 收集就诊于中国医学科学院肿瘤医院的局部进展期直肠癌患者57例,均于术前接受新辅助放化疗,并进行根治性手术.所有患者新辅助放化疗前后行经直肠腔内超声(ERUS)及剪切波弹性成像(SWE)检查.以术后病理为金标准,评价ERUS及SWE对直肠癌新辅助放化疗后T分期的准确性.结果 ERUS对局部进展期直肠癌新辅助放化疗后T分期的诊断准确率为59.6％(34/57).ERUS判断肿瘤局限于肠壁(T0～T2)的准确性为39.3％(11/28).46.2％(6/13)的ypT0期患者被过高分期.放化疗前后,杨氏模量最大值(Emax)平均值分别为(103.27±29.23)kPa、(49.91±32.67)kPa,差异有统计学意义(t=6.236,P<0.001).新辅助治疗后病理T分期越高,Emax平均值随之升高.以放化疗后病灶Emax构建ROC曲线,选取58 kPa作为最佳诊断临界点时,诊断放化疗后残余病变局限于肠壁内的敏感性、特异性、准确性分别为76.2％、86.4％、78.9％;以43 kPa为最佳诊断临界点,诊断ypT0期的敏感性、特异性、准确性分别为100％、84.6％、89.7％,均较ERUS有改善,说明SWE可提高新辅助治疗后直肠癌再分期的准确性,有助于临床治疗方案的制定.结论 经直肠腔内SWE可提高局部进展期直肠癌新辅助放化疗后T分期的准确性,可丰富现有影像检查方法.

目的 探讨直肠间质瘤腔内超声及超声造影表现.方法 回顾性收集2013年2月至2019年2月于中国医学科学院肿瘤医院就诊的直肠间质瘤患者的临床资料,包括临床表现,耦合剂充盈法腔内超声、超声造影、CT、MRI、肠镜检查结果等.按照间质瘤危险度分级将患者分为极低危、低危、中危、高危4个级别,分析不同级别超声造影检查特点,并比较不同检查方式诊断直肠间质瘤的准确率.结果 共12例符合纳入和排除标准的患者入选本研究,其中男性8例,女性4例,中位年龄54.0(50.5,65.25)岁.极低/低危组6例,中/高危组6例.肿瘤最大直径0.8～11.2 cm,腔内超声表现为向肠腔外生长的类圆形(7例,58.3％)或分叶状(5例,41.7％)低回声肿块,边界多较清晰(11例,91.7％),内部回声多不均匀(11例,91.7％),较大者可见囊变区(5例,41.7％),内部血流信号多较丰富(11例,91.7％).超声造影增强时相表现为动脉期不同程度快速显著增强[上升时间(8.10±4.03)s],并于动脉期或静脉期达峰[达峰时间(25.54±12.16)s],随后造影剂逐渐缓慢退出;中/高危组5例(83.3％)患者造影剂呈不均匀分布、向心性增强,且存在造影剂未灌注区;而极低/低危组仅1例(16.7％)造影剂呈不均匀分布或存在造影剂未灌注区,且6例(100％)均为弥漫性增强.与CT(60.0％)、MRI(66.7％)、肠镜(0)相比,腔内超声联合超声造影检查(100％)对直肠间质瘤的诊断准确率更高.结论 耦合剂充盈法腔内超声联合超声造影技术可很好地显示肿瘤起源和血管灌注情况,有助于明确诊断直肠间质瘤,且对直肠间质瘤的危险度分级具有提示意义.

BACKGROUND:Although primary thyroid lymphoma (PTL) and anaplastic thyroid carcinoma (ATC) both account for a rare portion of the morbidity of all thyroid malignancies, the therapeutic methods and prognosis for these two diseases are different. The purpose of this study was to investigate the sonographic characteristics of PTL and ATC and to compare the sonographic findings of PTL and ATC.METHODS:The study included 42 patients with histopathologically proven PTL (n=27) and ATC (n=15). The Clinical characteristics and sonographic findings were retrospectively reviewed and compared between the two groups.RESULTS:The mean age of patients with ATC was not significantly different from that in patients with PTL (P=0.601). The female-to-male ratio of patients with ATC was significantly lower than that of patients with PTL (P=0.029). Both PTL and ATC commonly present as a relatively large, solid mass on sonography with compressive symptoms, in which hoarseness was seen more frequently in ATC group (66.7%) than in PTL group (14.8%) (P=0.001). There is no significant difference in thyroid size, nodular size, margin, shape, echo texture, echogenicity, cystic change, vascularity and local invasion on sonography between ATC and PTL groups. Echogenic strands, markedly hypoechoic and enhanced posterior echo were seen more frequently in PTL group (92.6%, 92.6%, and 85.2%, respectively) than those in ATC group (6.7%, 60.0%, and 33.3%, respectively) (P<0.05), and calcification was seen more frequently in ATC group (80.0%) than in PTL group (0%) (P<0.001). Three ultrasound patterns were observed for PTL including diffuse type (25.9%), nodular type (48.2%) and mixed type (25.9%), while all ATC cases presented with nodular type (100.0%). Associated Hashimoto's thyroiditis occurred more frequently in PTL group (59.3%) than in ATC group (20.0%) (P=0.023).CONCLUSIONS:Certain sonographic features as a markedly hypoechogenicity, the presence of an enhanced posterior echo and linear echogenic strands, lack of calcification and associated Hashimoto's thyroiditis were valuable for distinguishing PTL from ATC. In contrast, heterogeneous echogenicity, uncircumscribed margin, irregular shape, and vascular pattern were not specific features for differential diagnosis.

Objective To investigate the sonographic features of metastatic carcinoma of the thyroid gland. Methods The ultrasonographic and histopathological data of 11 cases with metastatic carcinoma of thyroid gland were analyzed retrospectively. The ultrasonic types of metastatic carcinoma of the thyroid gland were classified according to the appearances of ultrasonography. In the 11 cases, there were 8 males and 3 females with a mean age of 62.3 ±6.3 years, aged from 49~73 years old. Results In 11 the cases, six patients had unilateral thyroid involvement and 5 had bilateral thyroid involvement. Five cases showed single nodule type (from lung in 2 cases, and from esophagus, kidney, and rectum 1 for each). The range of the largest diameter of lesions was from 3.0 to 5.6 cm (mean 3.9 cm). 4 cases showed multiple nodules type (from esophagus, kidney, rectum, and tongue 1 for each). The range of the largest diameter of lesions was from 1.5 to 3.8 cm (mean 2.5 cm). Two cases showed diffuse type (from lung and breast 1 for each). The single nodule and multiple nodules type presented as hypoechoic lesion with heterogeneous echo, irregular, ill defined contour, abundant flood and cervical lymph node enlargement, without halo, cystic degeneration and calcification. The diffuse type presented as hypoechoic lesion with diffused enlargement of the gland, heterogeneous echo, and abundant flood. All of them had cervical lymph node enlargement. Result The metastatic carcinoma of the thyroid gland has certain characteristics on ultrasonography. The ultrasonography is valuable in the diagnosis of the metastatic carcinoma of the thyroid gland when combining patient’s clinical history.

Background: Contrast-enhanced ultrasound (CEUS) permits evaluation of intratumoral vascularity in real time. The purpose of the present study was to compare the performance of conventional ultrasound (US) and CEUS in the evaluation of malignancy risk in patients with gastrointestinal stromal tumors (GISTs). Methods: In this retrospective study, we included patients with suspected GISTs who underwent conventional US and CEUS preoperatively. Fifty patients with pathologically confirmed GISTs were enrolled. Tumor risk stratifications were determined according to the 2008 NIH criteria. Pre-and post-CEUS findings were reviewed. Tumor size, lobular border, heterogeneity, cystic areas, calcification and intratumoral gas on US, irregular vessels, heterogeneous enhancement, and non-enhancing areas on CEUS were compared among different risk groups of GISTs. The relationship between time-intensity curve parameters and risk stratification was also analyzed. Results: Larger lesion size, lobulation, and cystic areas were associated with higher risk (P<0.05); however, margin, heterogeneity, calcification or intratumoral gas on US did not show significant association with tumor risk (P>0.05). Visualization of intratumoral irregular vessels and non-enhancing areas on CEUS were significantly correlated with higher risk classification (P<0.001), but heterogeneous enhancement was not. Quantitative analysis of time-intensity curve by CEUS showed that higher enhanced intensity (EI) was correlated with higher tumor risk, whereas arrival time (AT), time to peak enhancement (TTP), and area under the curve (AUC) were not correlated with tumor risk. Conclusions: The combination of conventional US and CEUS provides valuable information for preoperative risk stratification of GISTs.

Objective To study the ultrasonographic features and their corresponding pathological appearances of primary thyroid lymphoma (PTL). Methods The ultrasonographic features of 29 cases with PTL were analyzed and compared with the result of pathology by using the color Doppler ultrasound scanner and the histological and immunohistochemical staining sections. In the 29 cases, there were 3 males and 26 females with a median age of 67 years old, which aged from 50 to 83 years. Results In 29 cases, 15 patients had bilateral thyroid involvement, 13 patients had unilateral thyroid involvement and 1 patient had isthmus involvement. The sonographic findings of primary thyroid lymphoma could be classified into:diffuse type (9/29), diffuse type with multiple nodular type (12/29), mass type (7/29), and multiple nodular type (1/29). In 29 cases, 21 cases (72.4%) have cervical lymph node enlargement. Each pathologic type of PLT had its specific sonographic appearances. The mucosa associated lymphoid tissue node extranodal marginal zone B-cell lymphoma showed diffuse type on ultrasonography (3/4);The mixed type lymphoma showed diffuse type with multiple nodules (7/7);The diffuse large B-cell non Hodgkin’s lymphoma showed diversity on ultrasonography (5 cases of diffuse type, 4 cases of diffuse type with multiple nodules and 7 cases of mass type);And the full of T cell/tissue cells large B cell lymphoma showed diffuse type. Conclusions Different pathological types of PTL have their specific characteristics on sonography.

ObjectiveTo investigate the diagnostic value of endorectal ultrasonography in rectal tumors with sterile coupling gels filling the rectum.MethodsSixty patients with rectal tumors underwent endorectal ultrasonography with sterile coupling gels filling the rectum. The ultrasound findings were compared with histological diagnosis.ResultsThere were 8 patients with rectal adenoma, 40 patients with rectal cancer and 12 patients with rectal stromal tumors.The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of endorectal ultrasonography for rectal tumors was 7.27%, 100%, 100%, 94.2%, 95.0%, respectively.ConclusionEndorectal ultrasonography with sterile coupling gels filling the rectum is a valuable diagnostic method for rectal tumors and to provide reference for clinical management.

Objective To investigate the diagnostic value of ultrasound in thyroid cancer in children.Methods The ultrasonographic features of 27 patients with renal metastases confirmed by biopsy or surgical pathology or follow up were reviewed.Results A total 33 cases of the kidney were identified in the 27 patients.19 of these 33 cases were both unilateral and solitary;2 were unilateral and diffuse,and the rest 12 cases were bilateral and multiple.73.7％ (14/19) of solitary lesions were located at either pole of the kidneys.22 cases were detected by ultrasound and 11 cases were missed.The rate of missed diagnosis was 33.3％.The renal metastases were more often irregular shape(13/22),ill defined(15/22),exophytic(13/22),higher or close to the echo of the renal cortex(20/22),heterogenous(18/22),with or without cystic degeneration(12/22).There was no significant difference in the sonographic features of renal metastases derived from lung cancer and esophageal cancer.Conclusions In the detection and diagnosis of renal metastasis,ultrasound has certain limitations.Thus,as for the new renal lesion in patients with a history of primary tumor,we should be vigilant the possibility of metastasis.

Background: With the development of tyrosine kinase receptor inhibitor target therapy for gastrointestinal stromal tumors (GISTs), pre-treatment histopathological and immunocytochemical diagnosis of GISTs becomes important for clinical management. The purpose of this study was to compare the diagnostic accuracy of conventional ultrasound (US) guided vs. contrast-enhanced ultrasound (CEUS)-guided core needle biopsy for GISTs.Materials and methods: Between September 2011 and July 2015, 53 GIST patients underwent 61 conventional US guided or CEUS guided core needle biopsy at the Cancer Hospital & Institute, Peking Union Medical College & Chinese Academy of Medical Sciences. The outcomes of the biopsies were analyzed.Results: The diagnostic yield of CEUS guided biopsy group (96.2%, 27/28) was higher than conventional US guided biopsy group (78.8%, 26/33; P=0.042). The risk of undeterminable biopsy specimens in CEUS group (7.2%, 2/28) was lower than conventional US group (27.3%, 9/33; P=0.042). In both groups none patients had significant complications such as bleeding, pain, perforation or peritonitis after the biopsy.Conclusions: CEUS guided core needle biopsy for the diagnosis of GIST improved the diagnostic yield and therefore the pre-treatment risk assessment for GIST. The inclusion of CEUS guided biopsy in the diagnostic work-up of advanced or metastatic GIST is recommended.

Objective To investigate the value of ultrasonography in the diagnosis and differential diagno-sis of lymphoma and stromal tumor of the small intestine. Methods The clinical characteristics and ultrasound findings of 36 patients with small intestine lymphoma and 69 patients with small intestine stromal tumor confirmed pathologically in Cancer Hospital of Chinese Academy of Medical Sciences between January 2009 and January 2016 were retrospectively analyzed and compared. Results There were statistically significant differences in echotexture,presence of echoless area,intratumoral gas,and ultrasonographic pattern between small intestine lymphoma and small intestine stromal tumor( all P ﹤0. 001 ),whereas no statistically significant difference in clinical characteristics,lesion size,contour,or lobulated mass. On ultrasound,small intestine lymphoma mainly&nbsp;demonstrated as infiltrative or mass-forming type,with homogeneous echotexture without cystic necrotic area;while small intestine stromal tumor demonstrated as mass-forming type with heterogeneous echotexture and cystic necrotic area. Conclusions Lymphoma and stromal tumor of the small intestine both have some characteristic ultrasound features,the difference between which may help in the diagnosis and differential diagnosis of these two conditions.

Objective To investigate the value of ultrasonography (US) in estimating the risk of malignancy in small intestinal stromal tumors. Method The US features were evaluated retrospectively in 55 patients with patholog-ically and immunohistochemically confirmed small intestinal stromal tumors, who were divided into high-risk (n =44), moderate-risk (n = 3), and low-risk (n = 8) of malignancy groups based on mitotic activity, location and size of the tumors. The US features included tumor size, lobulation, heterogeneity, cystic change, hyperechoic foci, calcifica-tion and color Doppler flow. The correlation of US features with the risk of malignancy was evaluated using either Fisher ’s exact test or ANOVA. Result The maximum diameter of the tumors was 3.5 - 28.0 cm, and the mean di-ameters of tumors were 4.3 cm, 7.2 cm, 11.2 cm in low-, moderate-, and high-risk groups, respectively. The propor-tions of heterogeneity, lobulation, cystic change in low-risk group were less than that of moderate- and high-risk group (25% vs 100.0% vs 88.6%, 37.5% vs 100.0% vs 86.4%, and 25.0% vs 66.7% vs 81.8%), indicating a correla-tion with the risk of malignancy (P < 0.05). Tumor size, heterogeneity, lobulation and cystic changes differed signifi-cantly among groups (P < 0.05). Hyperechoic foci appeared more in moderate- (1/3, 33.3%) and high-risk group (8/44, 18.2%) than in low-risk group (0/8, 0); and abundant color signals were more frequent in high-risk group (17/44, 38.6%) than in intermediate-risk group (1/8, 12.5%) and low-risk group (0/8, 0), but without significant difference. Conclusion Certain US features are associated with increased risk of malignancy in small intestinal stromal tumors.

目的 探讨超声对原发性小肠淋巴瘤的诊断价值.方法 对29例原发性小肠淋巴瘤的声像图特征和病理结果进行对照分析.结果 单发13例,多发16例,位于回肠者最多.声像图分为4型:浸润型(5例)、肿块型(13例)、肠系膜型(2例)、混合型(8例);29例均为非霍奇金淋巴瘤,B细胞淋巴瘤26例,T细胞淋巴瘤3例.超声诊断准确度75.9％.结论 原发性小肠淋巴瘤超声表现具有一定特点,超声检查在其诊断中具有较高价值.

The primary aim of this study was to investigate the relationship between contrast-enhanced ultrasonography (CEUS) imaging parameters and clinicopathological features of rectal carcinoma and assess their potential as new radiological prognostic predictors. A total of 66 rectal carcinoma patients were analyzed with the time-intensity curve of CEUS. The parameter arrival time (AT), time to peak enhancement (TTP), wash-in time (WIT), enhanced intensity (EI), and ascending slope (AS) were measured. Microvessel density (MVD) was evaluated by immunohistochemical staining of surgical specimens. All findings were analysed prospectively and correlated with tumor staging, histological grading, and MVD. The mean values of AT, TTP, WIT, EI, and AS value of the rectal carcinoma were 10.84±3.28 s,20.61±5.52 s,9.78±2.83 s,28.68±4.67 dB, and 3.20±1.10, respectively. A positive linear correlation was found between the EI and MVD in rectal carcinoma (r=0.295,P=0.016), and there was a significant difference for EI among histological grading (r=-0.264,P=0.007). EI decreased as T stage increased with a trend of association noted (P=0.096). EI of contrast enhanced endorectal ultrasonography provides noninvasive biomarker of tumor angiogenesis in rectal cancer. CEUS data have the potential to predict patient prognosis.