目的:探讨二甲双胍联合炔雌醇环丙孕酮治疗多囊卵巢综合征(PCOS)的效果.方法:选取我院2017年1月至2018年1月PCOS病例90例,以随机数表采用简单随机分组方法分为对照组(口服炔雌醇环丙孕酮)和观察组(口服炔雌醇环丙孕酮+二甲双胍),每组45例,进行相应治疗,记录两组患者临床资料,检测治疗前后患者血清半乳糖凝集素-3(Galectin-3)水平、性激素、血脂、空腹血糖和胰岛素水平等指标.结果:治疗前,观察组和对照组各指标比较均无显著性差异(P>0.05);治疗后,观察组体质量指数(BMI)、腰臀比(WHR)、Galectin-3、黄体生成素(LH)、睾酮(T)、雌二醇(E2),甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、空腹血清葡萄糖(FPG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)治疗前后差值及排卵率、妊娠率均显著高于对照组,流产率均显著低于对照组,差异均有统计学意义(P<0.05);观察组卵泡刺激素(FSH)、总胆固醇(TC)与对照组比较,差值均无显著性差异(P>0.05).结论:二甲双胍联合炔雌醇环丙孕酮对PCOS有较好的治疗效果,可有效调节性激素水平、缓解胰岛素抵抗.

目的 研究6-酮-前列腺素F1α(6-k-PGF1α)、血栓素B2(TXB2)在硫酸镁联合低分子肝素治疗子痫前期中的作用和意义.方法 选取该院2015年5月至2017年5月收治的子痫前期孕妇60例作为观察组,给予患者硫酸镁联合低分子肝素治疗2个疗程;另选取健康孕妇60例作为对照组.观察子痫前期孕妇临床治疗疗效;收集两组孕妇血清并采用酶联免疫吸附试验法检测其血清中TXB2和6-k-PGF1α 的表达;检测子痫前期孕妇治疗后血压 、凝血功能 、肾功能变化,并与对照组进行比较.结果 与对照组比较,观察组治疗前血压(收缩压 、舒张压)和肾功能指标尿素氮 、24 h尿蛋白显著升高,血小板计数(PLT)、血D-二聚体(DD)水平和凝血酶原时间(PT)显著升高,TXB2显著降低,6-k-PGF1α 水平显著升高,差异均有统计学意义(P<0.05).轻度PE、晚发重度PE孕妇血中sCD40L和sFlt-1水平与早发重度PE呈显著正性相关关系,sVEGFR2和PLGF水平与早发重度PE呈显著负相关关系(P<0.05).与治疗前比较,观察组应用硫酸镁联合低分子肝素治疗后,收缩压 、舒张压 、尿素氮和24 h尿蛋白及PLT、血DD、PT均显著降低,TXB2显著升高,6-k-PGF1α 显著降低,差异均有统计学意义(P<0.05).结论 子痫前期孕妇应用硫酸镁联合低分子肝素治疗后疗效显著,6-k-PGF1α 、TXB2可以作为子痫前期预后良好的临床预测因子.

Astragalus polysaccharide (APS) is a natural compound extracted from astragalus membranaceus which has an antidepressant activity. Initially APS was studied for its immunomodulatory potential, and later was found to exhibit multiple pharmacological effects, including anti-inflammatory activity. More recently APS was shown to attenuate the lipopolysaccharide (LPS) induced neuroinflammation and improve the learning and memory ability of rats. The major objectives of this study were to investigate whether APS would exhibit antidepressant effects in an animal model of depression induced by LPS, and whether this effect might be associated with regulating nuclear factor-kappaB (NF-kappa B) and mitogen-activated protein kinase (MAPK) signaling pathways. Three groups of Wistar rats were injected LPS (i.p.), two groups of which were pretreated with APS (200 mg or 400 mg, i.p.). Behaviors were evaluated by forced swim test, saccharin preference test and open field test. Levels of NF-kappa B p65, phospho-NF-kappa B p65, phospho-I kappa B alpha, ERK1/2, JNK, p38 MAPK, phospho-ERK1/2, phospho-JNK and phospho-p38 MAPK in hippocampus and hypothalamus were measured to assess the activities of NF-kappa B and MAPK signaling pathways. In addition, levels of TNF-alpha, IL-1 beta and IL-6 (both protein and mRNA levels) in hippocampus and hypothalamus were determined. Results showed LPS induced depressive behaviors, as well as activated the NF-kappa B and MAPK signaling pathways in rats. APS treatment dose-dependently alleviated depressive-like symptoms and inhibited the activation of NF-kappa B and MAPK signaling pathways induced by LPS. The data indicate an antidepressant-like activity of APS in a LPS-induced animal model of depression possibly via inhibition of NF-kappa B and MAPK signaling pathways.

目的:研究血栓素A2(TXA2)及磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)信号通路在早发型子痫前期的作用和意义.方法:选取本院2015年5月至2017年5月收治的60例子痫前期孕妇为观察组,同期30例正常孕妇为对照组,采用免疫组织化学法检测两组胎盘组织中TXA2的表达,采用Caspase 3活性检测试剂盒检测凋亡蛋白Caspase 3表达,采用Real-time PCR和Western blotting检测患者血清和胎盘组织中PI3K、AKT的基因和磷酸化蛋白的表达.结果:与对照组相比,观察组患者血清TXA2、PI3K、AKT含量明显升高,Caspase 3活性明显升高,差异具有统计学意义(P＜0.05);观察组患者胎盘组织内TXA2、PI3K和AKT的蛋白和mRNA表达显著升高,差异具有统计学意义(P＜0.05).与对照组相比,观察组患者胎盘组织内TXA2蛋白平均光密度水平明显升高,差异具有统计学意义(P＜0.05).结论:早发型子痫前期患者体内TXA2呈异常过表达状态,且PI3K/AKT信号通路在早发型子痫前期的发病过程中具有重要的作用.

目的 研究血栓素A2(TXA2)、转录因子激活蛋白(AP-2α)、内皮素-1(ET-1)和 β-连环素(β-cate-nin)在子痫前期的作用和意义.方法 选取该院2015年5月至2017年5月收治的子痫前期孕妇60例,其中轻度子痫前期组 、早发重度子痫前期组和晚发重度子痫前期组各20例,另选取健康孕妇30例为对照组.收集受试者血清并采用酶联免疫吸附试验(ELISA)检测血清中TXA2、AP-2α 、ET-1和 β-catenin的表达;采用Real-Time PCR法检测血清中TXA2、AP-2α 、ET-1和 β-catenin基因表达.结果 与对照组比较,轻度子痫前期组 、早发重度子痫前期组和晚发重度子痫前期组患者TXA2、AP-2α 、ET-1和 β-catenin血清内水平和mRNA表达明显升高,差异具有统计学意义(P<0.05).与轻度子痫前期组比较,早发重度子痫前期组和晚发重度子痫前期组患者TXA2、AP-2α 、ET-1和 β-catenin血液内水平和mRNA表达明显升高,差异具有统计学意义(P<0.05).与早发重度子痫前期组比较,晚发重度子痫前期组患者TXA2和 β-catenin血清内水平和mRNA表达明显升高,AP-2α 和ET-1血清内水平和mRNA表达明显下降,差异具有统计学意义(P<0.05).轻度子痫前期组 、早发重度子痫前期组和晚发重度子痫前期组患者血清内TXA2、AP-2α 、ET-1和 β-catenin呈显著正相关关系(P<0.05).结论 子痫前期患者体内TXA2、AP-2α 、ET-1和 β-catenin呈异常过表达状态,且TXA2、AP-2α 、ET-1和 β-catenin与早发型子痫的严重程度具有明显的相关性.

目的 探讨胃癌干细胞CSC-G在胃癌侵袭和转移中的作用.方法 体外培养胃癌干细胞CSC-G和普通胃癌细胞SGC7901采用Western blot法检测两种胃癌细胞中干细胞标志物的表达情况,对比两种胃癌细胞的侵袭性检测结果和球形克隆形成情况.结果 胃癌干细胞CSC-G的干细胞标志物SOX2、OCT4的表达水平高于胃癌细胞SGC7901(P<0.05).球形克隆检测结果显示,胃癌干细胞CSC-G的瘤球数量为(65.49±10.22)个,多于胃癌细胞SGC7901的(7.36±1.14)个(P<0.05).侵袭能力检测结果显示,胃癌干细胞CSC-G穿过基底膜的数量为(168.71±32.50)个,多于胃癌细胞SGC7901的(92.44±13.06)个(P<0.05).结论 胃癌干细胞CSC-G在胃癌侵袭和转移过程中发挥着促进作用.

目的 探讨精细化护理在2型糖尿病肥胖患者饮食干预中应用的意义.方法 选取120例在本院内分泌科门诊及住院的2型糖尿病肥胖患者进行饮食干预,将患者随机分为观察组和对照组.对照组采用常规护理模式并进行饮食干预,观察组除常规护理外进行精细化饮食干预.比较两组患者体重增长情况、膳食营养摄取及并发症的发生率.结果 对照组体重增长指数高于观察组,观察组合理摄入膳食的情况优于对照组,并发症的发生率也低于对照组P<0.05.结论 在2型肥胖型糖尿病患者饮食干预中应用精细化护理模式,对饮食干预护理的各个方面进行细化,能更好地改善患者的生存状态,减少并发症的发生.

Non‐small cell lung cancer (NSCLC) comprises nearly 80% of lung cancers and the poor prognosis is due to its high invasiveness and metastasis. CC chemokine ligand 18 (CCL18) is predominantly secreted by M2‐tumor associated macrophages (TAMs) and promotes malignant behaviors of various human cancer types. In this study, we report that the high expression of CCL18 in TAMs of NSCLC tissues and increased expression of CCL18 in TAMs is correlated with the lymph node metastasis, distant metastasis, and poor prognosis NSCLC patients. CCL18 can increase the invasive ability of NSCLC cells by binding to its receptor Nir1. In addition, CCL18 is capable of modulating cell migration and invasion by regulating the activation of RAC1 which resulted in cytoskeleton reorganization in an ELMO1 dependent manner. Furthermore, we found that CCL18 could enhance adhesion of NSCLC cells via activating ELMO1‐integrin β1 signaling. Thus, CCL18 and its downstream molecules may be used as targets to develop novel NSCLC therapy. © 2016 Wiley Periodicals, Inc.

目的 探讨不同药物在宫颈上皮内瘤变(Cervical Intraepithial Neoplasia,CIN)环形电切除术(Loop ElectrosurgicalExcision Procedure,LEEP)术后的疗效.方法 将113例CIN患者随机分为辛复宁(重组人干扰素α-2b阴道泡腾胶囊)组、爱杰特(纳米银妇女外用抗菌凝胶)组和对照组,均行LEEP术,术后给予口服抗生素48 h预防感染.辛复宁组和爱杰特组分别给予辛复宁、爱杰特阴道后穹窿上药3个疗程,对照组常规处理,观察3组患者术后创面愈合时间、伴随症状持续时间、术后并发症、人乳头瘤状病毒转阴率.结果 辛复宁组、爱杰特组和对照组在愈合时间、伴随症状持续时间、并发症发生率、HPV转阴率等方面存在不同程度差异,其中辛复宁在以上各方面均存在优势.结论 CIN术后阴道局部用药治疗对术后恢复有积极意义;辛复宁明显缩短了创面愈合时间并减少了并发症的发生,提高了HPV转阴率,值得临床推广.

Aminopeptidase N (APN, also known as CD13) is involved in cellular processes of various types of tumors and a potential anti-cancer therapeutic target. Here, we report the effect of an APN inhibitor 4cc in enhancing sensitivity of hepatocellular carcinoma (HCC) cell lines and xenograft model in response to 5-fluorouracil (5-FU) in vivo and in vitro. The treatment of the combination of 4cc with 5-FU, compared to the combination of bestain with 5-FU, markedly suppressed cell growth and induced apoptosis of HCC cells, accompanying the increase in the level of reactive oxygen species (ROS) and followed by a decrease in the mitochondrial membrane potential (ΔΨM). Furthermore, the combination of 4cc and 5-FU showed a significant inhibitory effect on the growth of HCC xenograft tumors. In addition, following the treatment of 4cc, APN activity and clonogenic formation and the number of CD13-positive cells in PLC/PRF/5 cells were significantly decreased, suggesting that 4cc may also inhibit liver cancer stem cells by CD13 inhibition. These results showed that the APN inhibitor 4cc synergizes antitumor effects of 5-FU on human liver cancer cells via ROS-mediated drug resistance inhibition and concurrent activation of the mitochondrial pathways of apoptosis.

Determining cell quantity is a common problem in cytology research and anti-tumor drug development. A simple and low-cost method was developed to determine monolayer and adherent-growth cell quantities. The cell nucleus is located in the cytoplasm, and is independent. Thus, the nucleus cannot make contact even if the cell density is heavy. This phenomenon is the foundation of accurate cell-nucleus recognition. The cell nucleus is easily recognizable in images after fluorescent staining because it is independent. A one-to-one relationship exists between the nucleus and the cell; therefore, this method can be used to determine the quantity of proliferating cells. Results indicated that the activity of the histone deacetylase inhibitor Z1 was effective after this method was used. The nude-mouse xenograft model also revealed the potent anti-tumor activity of Z1. This research presents a new anti-tumor-drug evaluation method.

Aminopeptidase N (APN) is important in tumour processes. The present study detected the anti‑tumour activity of the novel APN inhibitor DH‑12a, which is an indoline‑2,3‑dione derivative. In the present study, Bestatin, a clinical APN inhibitor was used as a positive control. The expression of APN in the ES-2 and 3AO cell lines were assessed using flow cytometry and the drug inhibition constants of DH‑12a (Ki=13.15 µM) and Bestatin (Ki=16.57 µM) were assessed using a double reciprocal method of competitive inhibition. The in vitro effects of DH‑12a on cell proliferation were assessed using a 3‑(4,5‑dimethyl‑thiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide assay on human cell lines of ES‑2 (IC50=43.8 µM), A549 (inhibition rate=41.5% at 160 µM DH‑12a), HL60 (inhibition rate=47.83% at 160 µM DH‑12a) and 3AO (IC50=70.2 µM). The inhibition rates were consistently higher than those of Bestatin. The effects of DH‑12a on cell migration (inhibition rates in ES‑2 cells and 3AO cells were 56.4 and 76.5%, respectively at 15 µM) and invasion (inhibition rates in ES‑2 cells and 3AO cells were 75.6 and 66.5%, respectively at 15 µM) were assessed using transwell plates. The in vivo effects of DH‑12a on tumour proliferation and lung tumour metastasis were determined using an H22 xenograft mice model, where DH‑12a was administered in combination with genotoxic 5‑fluorouracil. The anti‑tumour activities of DH‑12a in vivo were also greater than those of Bestatin. In conclusion, the in vitro effects of DH‑12a on tumour proliferation, migration and invasion were consistent with the in vivo effects. In addition, DH‑12a exhibited greater anti‑tumour properties compared with Bestatin.

Multi-target drug design, in which drugs are designed as single molecules to simultaneously modulate multiple physiological targets, is an important strategy in the field of drug discovery. QT-011, a tamibarotene-furoxan derivative, was here prepared and proposed to exert synergistic effects on antileukemia by releasing nitric oxide and tamibarotene. Compared with tamibarotene itself, QT-011 displayed stronger antiproliferative effects on U937 and HL-60 cells and was more effective evaluated in a nude mice U937 xenograft model in vivo. In addition, QT-011 could release nitric oxide which might contribute to the antiproliferative activity. Autodocking assays showed that QT-011 fits well with the hydrophobic pocket of retinoic acid receptors. Taken together, these results suggest that QT-011 might be a highly effective derivative of tamibarotene and a potential candidate compound as antileukemia agent.

OBJECTIVE To investigate the improvement effect and mechanism of metformin(MF) on insulin resistance in castrated female rats.METHODS The levels of serum estradiol(E2),progesterone(P) and insulin(INS) were determined by radioimmunoassay method.Tumor necrosis factor-a(TNF-α) level was measured by enzyme-linked immunosorbent assay.Gene expression of pancreas islet suppressor of cytokine sighting-3(SOCS-3) was determined by using reverse transcriptase polymerase chain reaction.RESULTS Compared with NS group,the serum E2 and P content in OVX group were significantly decreased,the TNF-α,INS content were significantly increased,while the SOCS-3 mRNA expression of pancreas islets were significantly increased(P<0.01).Compared with the OVX group,MF low group has no significant change.Serum E2 and P content were significantly increased(P<0.05) in MF high group.The TNF-α,INS content significantly decreased(P<0.05 or P<0.01),while the SOCS-3 mRNA expression of islets were significantly decreased(P<0.01).CONCLUSION Long-term with a large dose of MF 270 mg?kg?1?d?1can improve ovariectormized rats insulin resistance phenomenon.The mechanism may be related with the decreased of TNF-α and SOCS-3 mRNA expression of islets.

Gliomas are characterized by high invasiveness and poor prognosis. Better understanding of the mechanism of invasion in glioma cells is essential to the design of effective therapy. Recently Grb2-associated binder 2 (Gab2), a member of the DOS/Gab family of scaffolding adapters, has been reported to play important roles in the development and progression of human cancers. However, it is not known whether Gab2 has any role in the migration and invasion of gliomas. This study attempts to investigate the association between Gab2 expression and progression of gliomas and the molecular mechanism of Gab2 in the glioma cell invasion. Methods. The expression of Gab2 in pairs of matched glioma tissues and their normal brain tissues was detected by Western blot. Immunohistochemistry was applied to evaluate the expression of Gab2 in 163 cases of histologically diagnosed gliomas. The invasive character of Gab2 decreased glioma cells and control glioma cells were investigated in vitro and in vivo in SCID mice brain. Results. Gab2 is found to be high expressed in gliomas and a subset of cancer cell lines. Statistical analysis suggested that the up-regulation of Gab2 correlated with the WHO grade of gliomas (p < 0.01) and that patients with high Gab2 expression levels exhibited shorter survival time (p < 0.01). In an animal experiment, knockdown of Gab2 through siRNA inhibited invasive ability of glioma cells into the brain of SCID mice. In cell research, reduction of Gab2 by siRNA inhibits the migration and invasion of glioma cells by mediating cytoskeleton rearrangement and MMPs expression. Additionally, IGF-1-induced pAkt and pmTOR phosphorylation was suppressed by the knockdown of Gab2. Conclusion. Gab2 may be a useful prognostic marker for gliomas and a novel therapeutic target for glioma invasion intervention.

The fast and diversified development of modern society requires medical technology talents to grasp the international cutting-edge information and the latest development,most of which come from english reports,so the importance of english teaching in medical colleges and universities is increasingly apparent.The teaching and research room of pharmacology of our university made a series of exploration in bilingual teaching reform by strengthening the integration of bilingual teaching and english case teaching and have made some achievements in the training of highly skilled bilingual medical technology talents.

OBJECTIVE:To optimize the extraction technology of diuretic ingredients in Luffae cylindrica.METHODS:The appropriate method for the diuretic ingredients extraction was chosen.The extraction technology of diuretic ingredients in L.cylindrica was optimized by orthogonal experiment with urine volume and itegracted assessment of percent of extracts of L.cylindrica as index and with concentration of alcohol,the extracting time and the amount of alcohol as factors.RESULTS:Alcohol backflow technology was the most appropriate method.The extracting efficiency was affected by the concentration of alcohol significantly,and the influence of the extracting time and the amount of alcohol had no significance.The optimal process is as follows:extracting for 2 times in 70% alcohol,8 folds alcohol,1.5 hour for the first time,and 1 hour for the second time.CONCLUSION:The method is simple,science,and coulde be provided the theory basis for pharmacological effects and clinical application of L.cylindrica.

<正>妇科恶性肿瘤主要的治疗手段是手术治疗。由于手术范围较广、创伤大、涉及盆腔诸多脏器,术后并发症多。因此,术后全面严格的护理是手术成败的关键。我科自2008年3月—2009年5月对116例妇科恶性肿瘤病人实施手术治疗,现将护理体会总结如下。

[目的]了解白内障病人围术期对健康教育内容及形式的需求,提高健康教育质量,创造安全的围术期环境。[方法]利用自行设计的健康教育需求调查表对我院眼科468例(588眼)行白内障超声乳化人工晶体植入术的病人术前术后进行问卷调查。[结果]病人需求率在90%以上的内容主要表现在治疗方法及疗程、手术方式及效果、并发症的发生及预防、疾病的预后与发展、术前术中术后指导、出院指导及自护方法、心理疏导等7个项目上。病人最喜欢的健康教育方式是医护合作讲解演示。[结论]加强围术期知识的宣教和指导是非常必要的,创造安全的围术期环境可以保证和巩固手术效果。

目的探讨psa、fpsa、fpsa/psa比值三项指标对前列腺癌早期诊断的意义。方法采用IRMA方法分别检测正常对照组、前列腺增生(BPH)和前列腺癌(Pca)组血清的psa、fpsa值,并计算fpsa/psa值进行统计学分析。结果BPH组、Pca组的psa和fpsa含量均高于正常对照组(p<0.01);BPH与Pca组在4<psa<10ng/ml时,fpsa/psa比值有显著性差异(p<0.05)。当psa>10ng/ml时BPH与Pca组PSA和FPSAR有显著性差异(p<0.01)。结论血清psa仍是前列腺癌的主要诊断手段之一,结合f/t比值可以更好的鉴别Pca和BPH,对Pca的早期诊断尤为重要。