Porcine rotavirus (PoRV), a major enteric pathogen, causes severe diarrhea, dehydration, high morbidity, and mortality, leading to significant economic losses in the porcine industry. In this study, we established a porcine small intestinal organoid culture system and developed an in vitro model for PoRV infection. Jejunal crypts were isolated from neonatal piglets and subsequently cultured to generate porcine small intestinal organoids in both three-dimensional (3D) and two-dimensional (2D) organoid formats, properties of which were verified via morphological assessment and immunostaining for Lgr5, villin, Ki-67, and lysozyme. Furthermore, a PoRV infection model was established in both 3D and 2D organoid cultures, and growth kinetics demonstrated efficient PoRV replication. PoRV infection also significantly increased the expression of inflammatory cytokines, including IL-6 and IFN-α. Collectively, these results demonstrate that the established porcine small intestinal organoid models support efficient PoRV replication and provide a physiologically relevant platform for studying PoRV pathogenesis and evaluating potential antiviral strategies.

Porcine rotavirus is one of the most important pathogens causing diarrhea in newborn piglets, and the genome of this virus contains 11 double-stranded RNA segments, which are easy to be recombined among strains to produce new strains with different antigenic properties. The reverse genetics system is an informative tool for studying virus biology. Recently, adaptable plasmid-based reverse genetics systems were developed for the porcine rotavirus OSU strain; however, such systems have not been developed for epidemic porcine rotavirus genotypes in China. In this study, we successfully established a reverse genetic system based on an epidemic strain of porcine rotavirus JXAY01 isolated in recent years, which was characterized by a specific genotype constellation: G5-P[23]-I12-R1-C1-M1-A8-N1-T7-E1-H1. 11 gene segments of porcine rotavirus JXAY01 were cloned into plasmid vectors similar to the SA11 system. JXAY01 genome segment plasmids were co-transfected with 10 complementary SA11 genome plasmids, and 11 monoreassortant strains were successfully rescued. Viral replication analyses of the parental SA11 strain and the monoreassortant strains showed that the structural protein replacement monoreassortants had reduced cell proliferation compared with the parental SA11 and non-structural protein replacement strains. The recombinant rJXAY01 strain could be successfully rescued using 11 pRG-JXAY01 plasmids. Whole genome sequencing showed 12 amino acid differences between the isolate JXAY01 and the recombinant rJXAY01, but there was no significant difference in their in vitro replication ability. This study reports the reverse genetic system, which lays the foundation for further understanding of porcine rotavirus molecular biology and novel vaccine development.

It is well established that PRRSV elimination is an effective strategy for PRRS control, but published reports concerning successful PRRSV elimination cases in farrow-to-finishing herds are rare. Here, we have reported a successful PRRSV elimination case in a farrow-to-finish herd by employing a "herd closure and rollover" approach with some modifications. Briefly, the introduction of pigs to the herd was stopped and normal production processes were maintained until the herd reached a PRRSV provisional negative status. During the herd closure, strict biosecurity protocols were implemented to prevent transmission between nursery pigs and sows. In the current case, introducing gilts before herd closure and live PRRSV exposure were skipped. In the 23rd week post-outbreak, the pre-weaning piglets started to show 100% PRRSV negativity in qPCR tests. In the 27th week, nursery and fattening barns fully launched depopulation. In the 28th week, nursery and fattening houses reopened and sentinel gilts were introduced into gestation barns. Sixty days post-sentinel gilt introduction, the sentinel pigs maintained being PRRSV antibody negative, manifesting that the herd matched the standard of the provisional negative status. The production performance of the herd took 5 months to bounce back to normal. Overall, the current study provided additional information for PRRSV elimination in farrow-to-finish pig herds.

[目的]确定江西省某猪场哺乳仔猪发生腹泻的病因.[方法]对送检的仔猪小肠样品进行猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)、猪传染性胃肠炎病毒(Transmissible gastroenteritis virus,TGEV)和猪轮状病毒(Porcine rotavirus,PoRV)的RT-PCR检测,将阳性样品接种MA104细胞传代进行PoRV的分离;对分离毒株进行电镜观察、间接免疫荧光试验、PoRV VP4和VP7基因序列测序和动物回归等试验.[结果]猪小肠病料样品经终浓度15 μg/mL的胰酶处理37℃孵育2 h,接种MA104细胞,能在细胞上增殖传代,第6代开始表现稳定的细胞病变;电镜观察可见病毒粒子直径大小为61～70 nm,平均大小为65 nm,呈带有短纤突且外缘光滑的形似车轮状的粒子,具有轮状病毒粒子典型的形态特征;间接免疫荧光试验和RT-PCR检测均为PoRV阳性,确定该分离株为PoRV.分离株VP4和VP7基因序列分析显示,VP4基因型与P[23]基因型相似性最高,VP7基因型与G5基因型相似性最高,根据A群轮状病毒最新分类方法,分离株属于G5P[23]型.动物回归试验结果显示,经口感染该分离株的1日 龄初生仔猪于感染后24 h左右陆续出现水样腹泻、呕吐等临床症状,并能在粪便中检测到PoRV.[结论]通过MA104细胞连续传代,从江西某猪场的腹泻仔猪小肠样品成功分离到1株PoRV,该分离株属于G5P[23]型PoRV,为哺乳仔猪发生腹泻的病原.

[目的]探寻鱼源乳酸菌与嗜水气单胞菌二者之间的相关性.[方法]采用琼脂扩散法开展体外抑制试验,比对法开展影响因素试验.[结果]鱼源乳酸菌对嗜水气单胞菌有较强的抑制作用;鱼源乳酸菌产酸强度越大,抑制作用越强;嗜水气单胞菌浓度越大,抑制作用越弱.[结论]鱼源乳酸菌可以抑制嗜水气单胞菌的生长,且对低浓度的嗜水气单胞菌抑制效果尤佳.

Rabbit Hemorrhagic Disease (RHD) is an economically significant infectious disease of rabbits, and its infection causes severe losses in the meat and fur industry. RHD Virus (RHDV) is difficult to proliferate in cell lines in vitro, which has greatly impeded the progress of investigating its replication mechanism and production of inactivated virus vaccines. RHDV VP60 protein is a major antigen for developing RHD subunit vaccines. Herein, we constructed a TK-deactivated recombinant Swinepox virus (rSWPV) expressing VP60 protein and VP60 protein coupled with His-tag respectively, and the expression of foreign proteins was confirmed using immunofluorescence assay and western blotting. Transmission electron microscopy showed that the recombinant VP60, with or without His-tag, self-assembled into virus-like particles (VLPs). Its efficacy was evaluated by comparison with available commercial vaccines in rabbits. ELISA and HI titer assays showed that high levels of neutralizing antibodies were induced at the first week after immunization with the recombinant strain and were maintained during the ongoing monitoring for the following 13 weeks. Challenge experiments showed that a single immunization with 106 PFU of the recombinant strain protected rabbits from lethal RHDV infection, and no histopathological changes or antigenic staining was found in the vaccine and rSWPV groups. These results suggest that rSWPV expressing RHDV VP60 could be an efficient candidate vaccine against RHDV in rabbits.

[目的]构建表达绿色荧光蛋白(green fluorescent protein,GFP)的重组猪痘病毒(recombinant Swinepox viruis,rSWPV),制备抗GFP单克隆抗体.[方法]首先合成3'-端含His标签的增强型绿色荧光蛋白(EGFP)序列EGFP-His,用双酶切方法将其插入到基础质粒载体pSW中,构建重组转移载体pSW-EGFP-His;采用脂质体转染的方法使该载体与SWPV(SWPV-JX20G株)同源重组,经蚀斑纯化获得rSWPV-EGFP-His.重组病毒进行PCR和SDS-PAGE鉴定,扩增并纯化EGFP-His蛋白免疫BALB/c小鼠,将小鼠脾细胞与SP2/0细胞融合,筛选分泌抗GFP特异性抗体的杂交瘤细胞,制备腹水,对抗GFP单克隆抗体进行效价及特异性鉴定.[结果]PCR和SDS-PAGE结果显示,成功构建并纯化到rSWPV-EGFP-His,该病毒感染PK15细胞可稳定表达EGFP-His蛋白,蛋白大小约27 ku,为可溶性表达,镍柱纯化的EGFP-His蛋白溶液呈明显的绿色.EGFP-His蛋白免疫BALB/c小鼠,免疫小鼠血清抗体效价高达1 ∶ 256 000.采用杂交瘤技术制备了 9株能稳定分泌抗EGFP-His单克隆抗体的杂交瘤细胞株,间接ELISA和Western blotting结果显示,9株单克隆抗体中的7株针对GFP的线性表位,另外2株单克隆抗体针对GFP的构象表位.[结论]本研究成功制备了 EGFP-His蛋白和9株抗EGFP-His的单克隆抗体,为后续建立GFP的免疫学检测方法提供了必备材料.

为建立用于检测临床粪便样品中胞内劳森菌(Lawsonia intracellularis,L.intracellularis)的TaqMan荧光定量PCR方法,本研究参考GenBank中发表的L.intracellularis PHE/MN1-00株基因组序列设计50对引物,通过SYBR Green Ⅰ荧光染料法PCR验证引物的特异性,针对扩增效率最好的特异性上、下游引物合成相应的TaqMan探针,优化反应条件,对其线性范围、敏感性和重复性进行验证,系统的完成了该方法的建立.结果表明,所建立的TaqMan荧光定量PCR方法,特异性强;在靶标核酸为2.95×101～2.95×106拷贝/μL区间线性关系良好,相关系数R2=0.998 1,扩增效率为96.51％;最低检测浓度为5拷贝/μL;批间和批内的CV均小于2％,重复性好.使用普通PCR方法与本方法对2020年10月至2021年2月采自江西地区猪场的373份粪便样品进行检测,L.intracellularis的总检出率分别为21.4％(80/373)和27.6％(103/373),显示本方法具有更高的敏感性,其检测场阳性率为88.9％(8/9),各阶段生产猪群阳性率分别为经产母猪28.7％、保育仔猪36.4％和育肥猪14.3％.该检测结果为江西地区的L.intracellularis的流行情况提供参考.

为了满足现代畜牧业、宠物业及其相关行业快速发展对兽医创新型人才的需求,从创新型人才的培养目标、培养方案的制定、创新型教师队伍的建设以及创新型人才评价体系的建立四个方面对高等农业院校兽医创新型人才培养模式进行分析和探讨,旨在为兽医创新型人才的培养提供行之有效的理论参考.

针对江西某猪场肥猪出现血便症状,取病变肠组织提取DNA,采用猪胞内劳森菌荧光定量PCR试剂盒检测,并对PCR扩增产物进行序列测定和分析,结果表明,其与胞内劳森菌参考序列(AM180252.1和CP004029.1)同源性为100％.同时采集7个猪场的正常猪群粪便通过猪胞内劳森菌的荧光定量PCR检测方法进行检测,结果显示,猪增生性肠炎在各猪场的感染率为16.7％～40％,平均感染率为30.06％.

为探究猪痘病毒作为载体表达猪圆环病毒2型(PCV2)-Cap蛋白,插入外源基因P28-ORF2的拷贝数与PCV2-Cap蛋白表达量的关系.本试验以猪痘病毒为载体,通过双酶切连接及无缝克隆方法构建重组质粒,并纯化到了8株含不同拷贝数(1～8拷贝)P28-ORF2的重组猪痘病毒,基于荧光斑大小、电镜观察病毒粒子及Western blotting结果进行判断.纯化到的8株重组病毒的荧光斑直径为317.41～384.96μm,大小无明显差异.重组猪痘病毒粒子形态与亲本病毒一致.Western blotting结果为插入1拷贝P28-ORF2的重组蛋白表达量最低;随着P28-ORF2拷贝数的增加,PCV2-Cap表达量也随之增加;至插入4拷贝P28-ORF2时,PCV2-Cap表达量达到峰值;随后减少.8株重组猪痘病毒荧光斑大小无明显差异,表明猪痘病毒基因组中插入不同拷贝数P28-ORF2对重组病毒在PK15细胞内的增殖无影响.重组猪痘病毒粒子的形态未发生变化说明多拷贝外源基因的插入并未对猪痘病毒的结构造成影响.本研究结果表明,猪痘病毒为载体表达外源蛋白时,单启动子启动多拷贝外源序列能明显提高外源蛋白的表达量,插入4拷贝的外源序列为较合适的拷贝数.

对某猪场送检的一份出现神经症状的保育仔猪病料进行了细菌分离,并对该分离菌株进行了染色镜检、生化鉴定、PCR检测和药敏试验.革兰氏染色结果表明从病料中分离到1株革兰氏阳性链状球菌,其生化鉴定结果与猪链球菌生化特性一致.分离菌株16srRNA基因测序结果表明其与2型猪链球菌亲缘关系最近,同源性达100％,确定该分离菌株为2型猪链球菌.药敏试验结果表明该分离菌株对青霉素、恩诺沙星、氨苄西林、氟苯尼考、头孢噻肟、阿莫西林、氧氟沙星、诺氟沙星等高度敏感;对复方新诺明、多西环素、土霉素、头孢曲松、丁安卡那等中度敏感,对磺胺异恶唑耐药.

Thousands of human deaths occur annually due to Japanese encephalitis (JE), caused by Japanese encephalitis virus. During the virus infection of the central nervous system, reactive gliosis, uncontrolled inflammatory response, and neuronal cell death are considered as the characteristic features of JE. To date, no specific treatment has been approved to overcome JE, indicating a need for the development of novel therapies. In this article, we focused on basic biological mechanisms in glial (microglia and astrocytes) and neuronal cells that contribute to the onset of neuroinflammation and neuronal cell damage during Japanese encephalitis virus infection. We also provided comprehensive knowledge about anti-JE therapies tested in clinical or pre-clinical settings, and discussed recent therapeutic strategies that could be employed for JE treatment. The improved understanding of JE pathogenesis might lay a foundation for the development of novel therapies to halt JE. Abbreviations AKT: a serine/threonine-specific protein kinase; AP1: activator protein 1; ASC: apoptosis-associated speck-like protein containing a CARD; ASK1: apoptosis signal-regulated kinase 1; ATF3/4/6: activating transcription factor 3/4/6; ATG5/7: autophagy-related 5/7; BBB: blood-brain barrier; Bcl-3/6: B-cell lymphoma 3/6 protein; CCL: C-C motif chemokine ligand; CCR2: C-C motif chemokine receptor 2; CHOP: C/EBP homologous protein; circRNA: circular RNA; CNS: central nervous system; CXCL: C-X-C motif chemokine ligand; dsRNA: double-stranded RNA; EDEM1: endoplasmic reticulum degradation enhancer mannosidase alpha-like 1; eIF2-ɑ: eukaryotic initiation factor 2 alpha; ER: endoplasmic reticulum; ERK: extracellular signal-regulated kinase; GRP78: 78-kDa glucose-regulated protein; ICAM: intercellular adhesion molecule; IFN: interferon; IL: interleukin; iNOS: inducible nitric oxide synthase; IRAK1/2: interleukin-1 receptor-associated kinase 1/2; IRE-1: inositol-requiring enzyme 1; IRF: interferon regulatory factor; ISG15: interferon-stimulated gene 15; JE: Japanese encephalitis; JEV: Japanese encephalitis virus; JNK: c-Jun N-terminal kinase; LAMP2: lysosome-associated membrane protein type 2; LC3-I/II: microtubule-associated protein 1 light chain 3-I/II; lncRNA: long non-coding RNA; MAPK: mitogen-activated protein kinase; miR/miRNA: microRNA; MK2: mitogen-activated protein kinase-activated protein kinase 2; MKK4: mitogen-activated protein kinase kinase 4; MLKL: mixed-linage kinase domain-like protein; MMP: matrix metalloproteinase; MyD88: myeloid differentiation factor 88; Nedd4: neural precursor cell-expressed developmentally downregulated 4; NF-κB: nuclear factor kappa B; NKRF: nuclear factor kappa B repressing factor; NLRP3: NLR family pyrin domain containing 3; NMDAR: N-methyl-D-aspartate receptor; NO: nitric oxide; NS2B/3/4: JEV non-structural protein 2B/3/4; P: phosphorylation. p38: mitogen-activated protein kinase p38; PKA: protein kinase A; PAK4: p21-activated kinase 4; PDFGR: platelet-derived growth factor receptor; PERK: protein kinase R-like endoplasmic reticulum kinase; PI3K: phosphoinositide 3-kinase; PTEN: phosphatase and tensin homolog; Rab7: Ras-related GTPase 7; Raf: proto-oncogene tyrosine-protein kinase Raf; Ras: a GTPase; RIDD: regulated IRE-1-dependent decay; RIG-I: retinoic acid-inducible gene I; RIPK1/3: receptor-interacting protein kinase 1/3; RNF11/125: RING finger protein 11/125; ROS: reactive oxygen species; SHIP1: SH2-containing inositol 5ʹ phosphatase 1; SOCS5: suppressor of cytokine signaling 5; Src: proto-oncogene tyrosine-protein kinase Src; ssRNA = single-stranded RNA; STAT: signal transducer and activator of transcription; TLR: toll-like receptor; TNFAIP3: tumor necrosis factor alpha-induced protein 3; TNFAR: tumor necrosis factor alpha receptor; TNF-α: tumor necrosis factor-alpha; TRAF6: tumor necrosis factor receptor-associated factor 6; TRIF: TIR-domain-containing adapter-inducing interferon-β; TRIM25: tripartite motif-containing 25; VCAM: vascular cell adhesion molecule; ZO-1: zonula occludens-1.

为了寻找番鸭肠道内优势益生菌株,用益生菌替代抗生素,将番鸭肠道细菌分离鉴定及体外抑菌试验.用微量生化法及16S rRNA基因序列检测分析法进行细菌分离鉴定;用纸片扩散法与牛津杯法做体外抑菌试验;用口腔灌服法进行动物安全性试验.结果显示,分离出的4株菌生化特性与乳酸杆菌基本一致,16S rRNA基因序列与唾液乳杆菌同源性大于99％;菌株生长速度快、产酸能力强,对胃蛋白酶、胆盐有一定的耐受性;对阿莫西林等敏感,对卡那霉素等耐药;对常见病原菌均有较强的抑制作用,上清液抑菌活性受pH和蛋白酶的影响,试验小鼠生长状况良好.说明分离菌具有广谱的抗菌效果,可以与卡那霉素等配合用药,作为微生态制剂用于多种病原菌疾病的防治,减少抗生素的使用.

兽医微生物学是一门面向动物医学、动物药学本科专业学生的专业基础课,掌握该门课程为后期学好其他专业课程奠定基础.为解决传统兽医微生物学教学过程存在内容繁杂、记忆和理解困难等问题,笔者将雨课堂工具应用于兽医微生物学教学过程中,将师生的智能终端联系起来,通过对课前、课上、课后每一个教学环节的设计与信息反馈,让教师及时掌握学生的学习动态,同时激发学生学习兴趣、提高学生的积极性和主动性,实现了师生之间良好的相互交流,提高了课堂教学效果,培养了学生独立思考、拓展思维等综合素质.

为筛选猪痘病毒（SWPV）复制非必需区并测定其缺失株的生物学特性,本研究根据同源重组原理分别针对SWPV的TK（ORF063）、ORF121和ORF143基因设计引物。应用重叠延伸PCR技术拼接同源重组左右臂及EGFP筛选标记表达盒,将拼接片段分别转染到感染SWPV的PK15细胞。利用荧光显微镜观察标记含EGFP的单个蚀斑,筛选获取重组毒株。应用PCR及Western blotting对重组毒株进行鉴定。分别测定各毒株感染PK15细胞的蚀斑大小和皮下接种保育猪致病性。PCR结果表明,目的基因成功整合到相应位点,成功获得重组毒株rSWPV-TK、rSWPV-121和rSWPV-143;Western blotting结果显示,连续传代的重组毒株在PK15细胞上稳定表达外源蛋白。重组毒株在PK15细胞上形成的痘斑均小于亲本毒株,其中ORF121缺失株差异极显著（P＜0.01）。各毒株均可导致保育猪痘斑形成,其中ORF121缺失株引起病变时间短,产生痘斑小,毒力较亲本下降。综上所述,本研究筛选到2个SWPV基因组中可供外源蛋白插入的复制非必需区ORF121和ORF143,为构建SWPV基因工程载体奠定了基础。

2017年4-7月份,江西某规模化猪场的育肥猪发生呼吸道疾病,为了找出病原,遏制病情扩散,试验对患病猪内脏进行细菌分离鉴定及体外抑菌试验,用微量生化法结合PCR法鉴定病原菌的种属,用琼脂扩散法进行药敏试验和抑菌试验,用腹腔注射法对小鼠进行攻毒试验.结果 表明:共分离得到6株分离菌,其中分离菌株JA1、JA2、JA4、JA5均不发酵糖类,M.R.、V-P、H2S等试验均为阴性,尿素、硝酸盐还原等试验均为阳性;分离菌株AP1、AP2发酵果糖、葡萄糖等,不发酵乳糖、鼠李糖等;分离菌株JA1、JA2、JA4、JA5与波氏杆菌同源性最高,分别为99.2％、99.4％、99.6％、99.5％;分离菌株AP1、AP2与胸膜肺炎放线杆菌同源性最高,分别为97.3％、98.4％.分离菌株JA1、JA2、JA4、JA5对青霉素G、氨苄西林、林可霉素等表现为耐药或中介,分离菌株AP1、AP2对恩诺沙星等表现为中介,对氟苯尼考等表现为敏感.乳酸菌(J2-100-R2、ZCR1-2)对分离菌有明显的抑菌作用,抑菌圈直径为17～26mm;注射分离菌的小白鼠36 h死亡率为66.67％,耐过小白鼠皮肤出现溃疡.说明该病是由支气管败血波氏杆菌与胸膜肺炎放线杆菌混合感染所致的呼吸道疾病;氟苯尼考与乳酸菌联合用药可以防治由支气管败血波氏杆菌与胸膜肺炎放线杆菌引起的呼吸道疾病.

为掌握江西省猪伪狂犬病病毒(PRV)的分子流行病学及遗传变异情况,本研究运用实验室已建立的PCR方法对2013～2018年采集自江西南昌、宜春、赣州、吉安、九江、上饶、抚州、新余8个地区的PRV阳性猪病料进行gE和gB基因扩增,并对PCR产物进行测序和序列分析.结果 显示,共获得64株PRV的gE基因序列和12株PRV的gB基因序列;gE基因遗传进化树表明,64株PRV同属一个大分支,与亚洲毒株及近年来国内分离株亲缘关系较近,全部为GⅠ型,而与GⅡ型的欧美经典毒株亲缘关系较远;江西毒株gE基因与19株参考毒株的核苷酸和氨基酸序列同源性分别为97.2％～100％和94.6％～100％;gB基因与11株参考毒株的核苷酸和氨基酸序列同源性分别为98.2％～I00％和96.5％～100％;相较于Bartha-K61疫苗株,江西流行毒株的gB基因存在碱基缺失、插入和位点突变现象.本研究从分子流行病学角度证实了江西省PRV的流行与变异情况,为江西省科学防控PRV提供理论依据.

为了寻找具有优势性能的乳酸菌,减少或替代抗生素在肉鸽养殖中的使用,试验采集2只健康肉鸽肠道内容物,用微量生化法结合分子生物学法进行细菌分离鉴定,用比对法进行生长特性试验,用琼脂扩散法进行体外抑菌试验,分离的乳酸菌腹腔接种小鼠进行菌株安全性试验.结果表明从2只健康肉鸽肠道内容物中分离得到4株生长迅速、产酸能力强的分离菌(分别标记为G2、G3、G4和G5),其生化特性与乳酸菌基本一致;分离菌16S rRNA核苷酸序列与唾液乳杆菌相似性最高,均大于99％;分离菌对氨苄西林等耐药,对罗红霉素高度敏感,对巴氏杆菌等指示菌均有较强的抑制作用;pH值越小分离菌G2发酵液抑菌活性越强,高温会略微降低抑菌活性但变化较小,酶可增加抑菌活性;腹腔接种分离菌发酵液的小鼠生长良好,未见异常.说明分离菌均为唾液乳杆菌,产酸能力强,耐胃蛋白酶和胆盐,对部分病原菌有较强抑制作用,安全无毒,具备成为益生菌的优势.

为了找到益生优势菌,扩大微生态制剂菌群资源,试验以获得的猪源益生肠球菌为研究对象,采用微量发酵管进行生化鉴定,PCR扩增法进行分子生物学检测,琼脂扩散法进行药敏试验和体外抑制试验,饮水法进行动物保护试验,对猪源益生肠球菌进行了筛选.结果 表明:分离出的6株菌ZDC1-80、Z2-80-R2、Z3-90-R1、Z2-90-R2、Z3-100-R1、Z3-100-R2均为有明显溶钙圈的革兰氏阳性球杆菌,除了棉籽糖,分离菌对大多数糖类都能发酵分解,1％马尿酸钠试验为阴性;分离菌在1 437 bp处出现目的条带,与肠球菌同源性最高,其中ZDC1-80、Z2-80-R2与希拉肠球菌同源性最高,Z3-90-R1、Z2-90-R2、Z3-100-R1、Z3-100-R2与粪肠球菌同源性最高;分离菌对阿莫西林等抗生素高度敏感,对诺氟沙星中等敏感,对链霉素等抗生素耐药;分离菌代谢物对病原指示菌均有抑制作用,对肠道病原菌抑制作用尤为显著;经过3周的观察,试验小鼠的生长状况良好.说明分离获得的猪源益生肠球菌具有广谱抗菌作用,其中Z3-100-R2的抑菌效果最强,可作为益生菌候选优势菌,与链霉素、克林霉素等配合用药,以减少抗生素的使用.