BACKGROUND:Preservation of normal-quality infrapatellar fat pads (IPFP) during total knee arthroplasty (TKA) has been proposed, and IPFP of abnormal quality are recommended for resection. METHODS:In this trial, patients with knee osteoarthritis (OA) undergoing TKA were stratified by IPFP status on magnetic resonance imaging (Group 1 with normal IPFP [signal intensity score ≤1; ranging from 0 to 3]; Group 2 with abnormal IPFP [signal intensity score ≥2]) and randomly assigned in a 1:1 ratio to IPFP preservation or IPFP resection. The primary outcome was 12-month change in the mean of five Knee Injury and Osteoarthritis Outcome Score (KOOS) subscales (KOOS5), which range from 0 (worst) to 100 (best). RESULTS:In Group 1, 179 participants were randomly allocated to IPFP preservation (n=90) and IPFP resection (n=89), and 198 participants were randomly assigned to IPFP preservation (n=101) and IPFP resection (n=97) in Group 2. The increases in KOOS5 score were 38.1 and 36.1 in Group 1 and were 39.2 and 37.5 in Group 2 in IPFP preservation and IPFP resection groups, respectively, resulting in between-group differences of 1.9 (95% confidence interval [CI], -1.7 to 5.6) in Group 1 and 1.6 (95% CI, -1.3 to 4.6) in Group 2. Adverse events were predominantly nonserious musculoskeletal or skin-related events (three vs. two in Group 1 and eight vs. two in Group 2). CONCLUSIONS:Resection of abnormal IPFP and preservation of normal IPFP did not improve postoperative outcomes 12 months after TKA. (Funded by National Key Research & Development Program of China, Clinical Research Startup Program of Southern Medical University, and National Natural Science Foundation of China; ClinicalTrials.gov number, NCT03763448.).

N6-methyladenosine (m6A) plays a critical role in osteoarthritis (OA) pathogenesis. This study investigates the role of the m6A reader insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) in OA progression. OA models were established in vivo through destabilization of the medial meniscus (DMM) surgery in mice and in vitro by stimulating primary chondrocytes with interleukin-1β (IL-1β). Cartilage destruction was assessed histopathologically. Chondrocyte viability, proliferation, and apoptosis were measured by CCK-8, EdU, and flow cytometry, respectively. Inflammatory cytokine levels, lactate, glucose, and total m6A were quantified using commercial kits. Expression levels of IGF2BP3, extracellular matrix protein 1 (ECM1), ECM turnover markers, and glycolysis-related genes were analyzed by RT-qPCR, Western blot, immunohistochemistry, and immunofluorescence. Potential m6A modification sites in ECM1 transcripts were predicted using the SRAMP database, and IGF2BP3-mediated, m6A-dependent regulation of ECM1 was validated through RNA immunoprecipitation (RIP), MeRIP-qPCR, and actinomycin D decay assays. Results revealed elevated levels of IGF2BP3, ECM1, m6A modification, and glycolytic activity in OA cartilage and IL-1β-treated chondrocytes. IGF2BP3 knockdown reversed IL-1β-induced reductions in chondrocyte viability and proliferation, suppressed apoptosis, decreased inflammatory cytokine secretion, attenuated ECM degradation, and reduced glycolytic flux in vitro, while also mitigating cartilage damage and matrix breakdown in vivo. Mechanistically, IGF2BP3 stabilized ECM1 mRNA in an m6A-dependent manner, thereby modulating ECM1 expression. Restoration of ECM1 expression abolished the chondroprotective effects of IGF2BP3 knockdown against injury, inflammation, and enhanced glycolysis. In conclusion, IGF2BP3 enhances ECM1 expression via an m6A-dependent pathway, thereby promoting glycolysis and exacerbating OA progression.

BACKGROUND:This study analyzes the disease burden of elderly knee osteoarthritis (KOA) and its risk factors in individuals aged 55+ years using Global Burden of Disease data from 1990 to 2021, aiming to inform public health policy and interventions. METHODS:Data from the Global Burden of Disease and Global Health Data Exchange were used to investigate age- and sex-specific trends in KOA incidence, prevalence, and disability-adjusted life years (DALYs). Age-standardized incidence rate (ASIR) and age-standardized disability rate were calculated, and global maps were created to visualize trends. A Bayesian Age-Period-Cohort model predicted the disease burden for 2050. Analyses were done using R software (v4.0.4). RESULTS:From 1990 to 2021, the number of new KOA cases and DALYs increased globally in both men and women, with higher ASIR and DALYs rates in women. ASIR rose across all sociodemographic index (SDI) strata, with the largest increase in low-middle SDI countries. Regionally, ASIR increased in most areas except high-income Asia Pacific. The highest disease burden was observed in the 55 to 59 years age group, with DALYs peaking in the 75 to 79 years age group. At the country level, China, Myanmar, and Malaysia had the highest incidence, whereas China, India, and the United States had the highest DALYs. High body-mass index emerged as a notable contributor to the disease burden in 2021. Projections indicate rising DALYs rates across all age groups by 2050, particularly in the oldest age groups. DISCUSSION:The global burden of elderly KOA has increased markedly over three decades, disproportionately affecting women. ASIR rose across all SDI strata, with the largest increase in low-middle SDI countries. These findings provide evidence for the development of elderly health intervention measures.

Background The acetabular distraction technique utilizing porous shells and augments is effective for chronic pelvic discontinuity. We report a modified technique employing a novel bispherical augment to achieve controllable distraction and enhanced reconstruction. Methods We conducted a retrospective case series of 15 consecutive revision total hip arthroplasties performed for the treatment of chronic pelvic discontinuity at a single institution between 2017 and 2021. A modular revision system of bispherical augment with a highly porous hemispherical shell was used for defect reconstruction and acetabular distraction. The mean follow-up was 5.4 years (4.0-8.1 years). Primary outcomes were implant survivorship free from aseptic loosening and radiographic healing of the discontinuity were assessed, and complications were documented. Results One patient (6.7%) required acetabular revision for aseptic loosening at 3.5 years. One patient required bearing exchange for recurrent dislocation at 3 months postoperatively. Radiographic healing of the discontinuity was achieved in 93.3% (14/15) of hips. One asymptomatic patient had a nonprogressive radiolucent line. Mean Harris scores improved significantly from 28.6 preoperatively to 76.3 at latest follow-up (P = .002). The hip center of rotation was significantly restored inferiorly (41.5 mm-24.4 mm, P = .003) and lower limb discrepancy corrected (24 mm-5 mm, P = .012). Cumulative survival free from revision for aseptic loosening was 90.9% (95% confidence interval: 74%-100%) at mean 5.4 years. No neurovascular complications occurred. Conclusions The application of a bispherical augment to enable a modified, controllable acetabular distraction technique provides a reliable alternative method for reconstructing chronic pelvic discontinuity; further larger cohorts and longer follow-up are needed.

Implant-related infections (IRIs) present a significant challenge in clinical treatment because of the formation of biofilms. The complex architecture of biofilms not only impedes antibiotic penetration, fostering the evolution of multidrug resistance in bacteria under minimal selective pressure but also suppresses the antimicrobial activity of macrophages and induces their pyroptosis in large quantities. This excessive pyroptosis impairs the collective immune function of macrophages, enabling pathogens to evade immune system clearance and rendering infection difficult to eradicate. Existing treatment strategies often necessitate extensive surgical debridement, which not only causes significant harm to patients' physiological health and quality of life but also results in limited therapeutic outcomes. To address these challenges, this study developed a mesoporous silica nanoparticle system (MRL) modified with the RGD (Arginine-Glycine-Aspartic acid) tripeptide and loaded with the antimicrobial peptide LL-37. The LL-37 released from MRL can not only directly disrupt bacterial cell membranes, preventing bacteria from developing resistance through conventional mutation mechanisms, but also enhance antimicrobial activity by modulating macrophage polarization toward the M1 phenotype. However, LL-37 may induce and exacerbate macrophage pyroptosis within biofilms. Therefore, we modified the nanoparticles with RGD to increase macrophage viability and reduce their number of deaths, thereby alleviating the immunosuppression caused by excessive macrophage pyroptosis. In vitro and in vivo experiments demonstrated that MRL, while preserving the antimicrobial activity and immunomodulatory function of LL-37, significantly reduced macrophage pyroptosis and protected the collective immune activity of macrophages. Thus, the fine-tuned regulation of immune response was achieved, providing new insights and strategies for the treatment of IRIs.

Novel bispherical augments developed for acetabular defect reconstruction, which combined with acetabular components, have shown excellent results in clinical follow-up. This study aims to evaluate the primary stability of them in severe acetabular defect reconstruction and biomechanical effect using different combine methods in the component-augment interface, and to further explain their value in clinical applications. Nine composite hemipelvis specimens were prepared with a Paprosky II defect. A porous titanium acetabular shell and bispherical augment were combined and implanted for acetabular defect reconstruction. The augment was fixed to the hemipelvis using screws, while three specimens per groups of three groups were varied by different fixation techniques between the augments and acetabular components: (1) screw fixation only, (2) cement fixation only, and (3) screw and cement fixation. The combined components and hemi pelvic specimens were cyclically loaded (500 cycles) with three different levels of loads (maximum 0.5 kN, 0.9 kN, and 1.8 kN). All constructs had micromotions of less than 99 μm, and bony ingrowth could be achieved between the prostheses and the bone in 3–30

In osteoporotic bones, the stability of orthopedic implants is compromised, and excessive M1 macrophage polarization at the bone-implant interface disrupts bone-immune homeostasis, leading to implant loosening or failure. To address this, this study develops a bionic magnesium alloy internal fixation coating inspired by the "brick-and-mortar" structure of pearl, aiming to improve bone-implant integration and vascularization in osteoporotic conditions. The multifunctional coating consists of a calcium phosphate (Ca-P) "brick" layer, which serves as a mineralization template and corrosion barrier, and fibronectin-mimetic peptides (Fn-mimetic peptides) as the "mortar" to promote cell adhesion, regulate immune responses, and stimulate angiogenesis. This bionic multilayer structure not only alleviates oxidative stress in the osteoporotic microenvironment but also fosters immune regulation-osteogenesis coupling and improves the bone-vascular-immune microenvironment. It precisely controls the degradation rate of Mg alloys and enhances tissue repair. The CaP layer reduces rapid degradation and prevents hydrogen gas release and local alkalinization, whereas Fn-mimetic peptides enhance early bone integration and vascularization. The synergistic effect of the magnesium alloy implant and bionic coating significantly improved bone implant stability, regeneration, and vascularization, as demonstrated in osteoporotic rat models, offering a promising strategy for the design of bone repair materials under pathological conditions.

Sensitive detection of microRNA-204 (miR-204) is critical for the early diagnosis and management of osteoarthritis (OA). This work presents a novel surface-enhanced Raman scattering (SERS) biosensor for the ultrasensitive and specific detection of OA-associated miR-204. The platform integrates a self-amplifying nucleic acid circuit with DNAzyme-catalyzed etching of a plasmonic nanoprobe. At its core is a single, rationally designed overhang-containing hairpin probe (O-HP) that functions as both the recognition element and amplification initiator. Upon binding to miR-204, the O-HP triggers polymerase-mediated extension, generating G-quadruplex structures. These structures bind hemin to form DNAzymes that catalyze the localized production of reactive oxygen species (ROS), which subsequently etch the silver shell of the gold nanostars core with silver shell and 4-aminothiophenol (AuNS/Ag@4-ATP) SERS nanoprobe. This etching causes the desorption of Raman reporters and a quantifiable 'signal-off' response. This biosensor achieves a remarkably low detection limit of 8.13 fM with a broad dynamic range from 10 fM to 150 nM, and exhibits high specificity, capable of discriminating single-nucleotide variants. Furthermore, it successfully quantified miR-204 in clinical cartilage samples, showing a strong correlation with real-time quantitative polymerase chain reaction results. The modular design of the O-HP also facilitated the adaptation of the platform for detecting miR-21, demonstrating its generalizability. This work provides a robust and versatile biosensing strategy with significant potential for clinical miRNA diagnostics.

Cysteine sulfenylation (Cys-SOH) is a transient redox-sensitive post-translational modification that regulates protein activity and cellular stress responses, yet its in vivo dynamics remain difficult to capture due to short half-life and low abundance. Here, we report the design and application of BTD-Az, a cell-permeable probe that incorporates an azide handle into the benzothiazine scaffold, enabling rapid bioorthogonal labeling of Cys-SOH both in vivo and in vitro, followed by efficient enrichment through strain-promoted azide-alkyne cycloaddition. This approach streamlines the detection and analysis of Cys-SOH with exceptional specificity and convenience. BTD-Az exhibited negligible cytotoxicity, efficiently enriched sulfenylated proteins in vitro, and achieved direct in vivo labeling in mouse tissues through SPAAC-mediated pull-down. Coupling BTD-Az with 4D-DIA proteomics allowed global sulfenylome profiling, revealing >5000 labeled proteins across tissues. As a proof-of-concept biological application, we applied BTD-Az to aging cartilage and identified 95 proteins with differential sulfenylation, including the mitochondrial enzyme IDH2, whose Cys-SOH modification promoted proteasomal degradation and exacerbated redox imbalance. Collectively, this study establishes BTD-Az as a robust chemical tool for in vivo sulfenylation profiling, providing a broadly applicable platform for redox proteomics and the discovery of redox-sensitive regulatory mechanisms in health and disease.

The surgical treatment of bone tumors generally causes bone defects, whilst postoperative infection and tumor recurrence may also occur. Currently, it is still a huge challenge to design multi-functional biomaterials that both eradicate residual tumor cells and bacteria whilst also promoting osteogenesis. Herein, hafnium oxide with an oxygen vacancy (HfO2-x) was synthesized via a deoxidation reaction and was shown to exhibit excellent photothermal performance. Moreover, a composite based on polyetheretherketone (PEEK) containing HfO2-x (PBHC) was fabricated and displayed good photothermal performance that effectively ablated the tumor cells and eradicated bacteria in vitro under 808 nm NIR laser irradiation. Moreover, the PBHC with good photothermal performance exhibited excellent ability for the treatment of tumors and infection in vivo. Further, PBHC significantly boosted the osteoblastic differentiation of bone mesenchymal stem cells in vitro and promoted bone regeneration in vivo due to the osteogenic activity of HfO2-x. PBHC is a multi-functional biomaterial with good biocompatibility, photothermal performance and pro-osteogenic properties that can overcome the triple challenges of tumor recurrence, bacterial infection and bone defects. In summary, PBHC with tumor/bacteria-eradicating and pro-osteogenesis effects could be applicable for the simultaneous treatment of tumors and infection as well as the repair of bone defects.

BACKGROUND:Crowe type IV developmental dysplasia of the hip (DDH) presents unique challenges in total hip arthroplasty. This study compares the clinical outcomes and efficiency of the direct anterior approach (DAA) in the lateral decubitus position combined with proximal femoral osteotomy versus the posterolateral approach (PLA). METHODS:A retrospective analysis of 73 hips from 64 patients who had Crowe type IV DDH was conducted. Patients underwent total hip arthroplasty via DAA or PLA. Clinical and radiological outcomes, including postoperative complications, radiological evaluations (implant alignment and osteotomy healing included), Harris Hip Score, limb length discrepancy, Visual Analog Scale pain scores, operative time, and intraoperative bleeding, were evaluated. In addition, the operation time and the learning curve of the DAA group were also evaluated. RESULTS:There was no significant difference in postoperative complications and radiological evaluations between the two groups. The DAA group achieved superior functional recovery, postoperative pain relief, and lower intraoperative bleeding than the PLA group. The DAA group had longer initial operative times, but showed significant reductions with experience. CONCLUSIONS:The lateral decubitus DAA combined with proximal femoral osteotomy offers superior functional recovery, pain relief, and reduced bleeding compared to PLA, with comparable implant stability. These findings support DAA as an effective option for Crowe type IV DDH. Further studies are warranted to confirm these results. LEVEL OF EVIDENCE:Level III.

The number of hip revision surgeries is expected to increase in recent years, and reconstruction of acetabular defects is a challenge for joint surgeons. The principle of reconstruction of acetabular defects is to achieve initial and long-term stability between the prosthesis and the host bone. With the development of surgical techniques, prosthetic materials, and revision concepts, there is an urgent need for new acetabular bone defect evaluation systems to meet clinical needs. The uncemented porous hemispherical cup has become the main prosthesis in clinical application, and metal augments are gradually replacing the structural allograft. Modular reconstruction combined cups and augments has shown favorable clinical results, which can be used for large acetabular defects with acetabular distraction technique, such as pelvic discontinuity. The advantages and disadvantages of impaction bone grafting, jumbo cups, metal augments, acetabular reinforcement rings, custom components (including custom triflanged acetabular components), and acetabular distraction technique still need to be observed in long-term follow-up.

This study used finite element analysis (FEA) to compare the biomechanical stability of bispherical metal augment (BA) and wedge-shaped trabecular-metal augment (TA) in different acetabular defect reconstruction models, thereby explaining the application value of this novel bispherical augment in complex hip revision. Three different acetabular defect pelvis models originating from three representative patients with different types of severe acetabular defects (Paprosky IIC, IIIA, and IIIB) were constructed and reconstruction with BA and TA technique was simulated. Based on the FEA models, the displacement of reconstruction implants, relative displacement of bone implants, and hemi-pelvic von Mises stress were investigated under static loads. BA acquired smaller reconstruction system displacement, less relative displacement of bone implants, and lower pelvic von Mises stress than TA in all Paprosky IIC, IIIA, and IIIB defect reconstructions. The FEA results show that BA could acquire favourable biomechanical stability in severe acetabular defect reconstruction. This technique is a reliable method in complex hip revision.

Implant-associated infections (IAIs) pose a significant threat to orthopedic surgeries. Bacteria colonizing the surface of implants disrupt bone formation-related cells and interfere with the osteoimmune system, resulting in an impaired immune microenvironment and osteogenesis disorders. Inspired by nature, a zeolitic imidazolate framework (ZIF)-sealed smart drug delivery system on Ti substrates (ZSTG) was developed for the "natural-artificial dual-enzyme intervention (NADEI)" strategy to address these challenges. The subtle sealing design of ZIF-8 on the TiO2 nanotubes ensured glucose oxidase (GOx) activity and prevented its premature leakage. In the acidic infection microenvironment, the degradation of ZIF-8 triggered the rapid release of GOx, which converted glucose into H2O2 for disinfection. The Zn2+ released from degraded ZIF-8, as a DNase mimic, can hydrolyze extracellular DNA, which further enhances H2O2-induced disinfection and prevents biofilm formation. Importantly, Zn2+-mediated M2 macrophage polarization significantly improved the impaired osteoimmune microenvironment, accelerating bone repair. Transcriptomics revealed that ZSTG effectively suppressed the inflammatory cascade induced by lipopolysaccharide while promoting cell proliferation, homeostasis maintenance, and bone repair. In vitro and in vivo results confirmed the superior anti-infective, osteoimmunomodulatory, and osteointegrative capacities of the ZSTG-mediated NADEI strategy. Overall, this smart bionic platform has significant potential for future clinical applications to treat IAIs.

Robust bacterial metabolism and the immunosuppression on peripheral immune cells cause biofilm-associated infections (BAIs) extremely refractory to be eradicated via antibiotics alone. Herein, hierarchical mesoporous UiO-66 metal-organic framework is decorated with selenite, polypyrrole, and macrophage membrane (MM) to develop a biomimetic nanosphere (USPM). Following the recruitment of USPM to the biofilm microenvironment (BME) via the pathogen-targeting ability derived from MM. The BME-responsive USPM can precisely release selenite to penetrate the loosened biofilm in synergy with near-infrared-induced mild photothermal therapy (mPTT). Selenite can quickly react with reducing substances to generate hydrogen selenide (H2Se) inside the biofilm. H2Se can competitively inhibit bacterial metabolic processes and disrupt biofilm metabolic homeostasis by cascade amplification effects. Furthermore, H2Se inside the biofilm further sensitizes photothermia to exert a precise local photothermal effect. Outside the biofilm, USPM can simultaneously promote the phagocytosis and autophagy of macrophages to kill and decompose the phagocytosed bacteria. Finally, the well-decomposed bacterial antigens in macrophages can be presented to antigen-presenting cells to arouse adaptive immune responses and enhance anti-biofilm effectiveness further. Such powerful mPTT-enhanced bacterial metabolic disruption and macrophagic autophagy-promoted adaptive immune activation suggest an alternative therapeutic strategy to cure refractory BAIs. Fabrication of USPM for treating BAIs via bacterial metabolic interference and autophagy-promoted immunity is presented. USPM releases selenite into the biofilm under acidity. Selenite reacts with reducing substances to yield H2Se, which exerts a disruptive effect on bacterial metabolic homeostasis. Furthermore, H2Se sensitizes photothermia within the biofilm. USPM stimulates M1 polarization and autophagic activation of macrophages and promotes the release of pro-inflammatory cytokines.image

Objective: To evaluate the clinical effect of bispherical augment in acetabular defects reconstruction in hip revision. Methods: This is a retrospective case series study. A retrospective analysis of 119 patients (124 hips) patients who underwent hip revision surgery and reconstructed with bispherical augment for acetabular bone defects from January 2019 to December 2023 was performed. There were 57 males (58 hips) and 62 females (66 hips), aged (65.0±11.8) years (range:40 to 102 years). The body mass index was (23.9±3.5) kg/m2 (range:16.1 to 32.2 kg/m2). Acetabular bone defects were typed as follows: 2 hips in Paprosky type ⅡA, 29 hips in type ⅡB, 34 hips in type ⅡC, 31 hips in type ⅢA, and 28 hips in type ⅢB, of which 9 patients (9 hips) were combined with pelvic discontinuity. Differences in Harris hip score (HHS) and lower limb discrepancy (LLD) were compared between preoperatively and final follow-up. The height of the hip center of rotation and the horizontal distance from the center of rotation to the teardrop were measured by radiographs before and after surgery, and prothesis stability and the occurrence of postoperative complications were evaluated. Data were compared using the paired sample t test. Results: All patients successfully completed the operation. The operation time was (167.0±53.4) minutes (range:90 to 380 minutes) and the intraoperative bleeding was (345.3±124.2) ml (range:100 to 1 200 ml). The height of the hip center of rotation decreased from (39.7±13.0) mm preoperatively to (21.8±7.1) mm postoperatively and the horizontal distance from the center of rotation to the teardrop increased from (34.0±10.1) mm preoperatively to (38.5±5.9) mm postoperatively, and the differences were statistically significant (t=15.859, P<0.01; t=5.266,P<0.01). All the patients were followed up for (26.1±15.4) months (range:6 to 60 months). At the last follow-up, HHS improved from (35.2±10.0) points preoperatively to (85.5±9.5) points, and the difference was statistically significant (t=50.723,P<0.01). LLD decreased from (2.1±1.1) cm preoperatively to (0.5±0.5) cm, and the difference was statistically significant (t=13.767, P<0.01). All acetabular components were stable and free of displacement on imaging during follow-up. Three patients suffered dislocation and received closed reduction, all prosthesis were in good position during follow-up. No dislocation, loosening, fracture, recurrence of infection and vascular nerve injury occurred in other patients. Conclusion: Bispherical augment can effectively reconstruct acetabular bone defects, restore the hip center of rotation, and improve hip joint function scores at short and mid-term follow-up.

Pathogen-host competition for manganese and intricate immunostimulatory pathways severely attenuates the efficacy of antibacterial immunotherapy against biofilm infections associated with orthopaedic implants. Herein, we introduce a spatiotemporal sono-metalloimmunotherapy (SMIT) strategy aimed at efficient biofilm ablation by custom design of ingenious biomimetic metal-organic framework (PCN-224)-coated MnO2-hydrangea nanoparticles (MnPM) as a metalloantibiotic. Upon reaching the acidic H2O2-enriched biofilm microenvironment, MnPM can convert abundant H2O2 into oxygen, which is conducive to significantly enhancing the efficacy of ultrasound (US)-triggered sonodynamic therapy (SDT), thereby exposing bacteria-associated antigens (BAAs). Moreover, MnPM disrupts bacterial homeostasis, further killing more bacteria. Then, the Mn ions released from the degraded MnO2 can recharge immune cells to enhance the cGAS-STING signaling pathway sensing of BAAs, further boosting the immune response and suppressing biofilm growth via biofilm-specific T cell responses. Following US withdrawal, the sustained oxygenation promotes the survival and migration of fibroblasts, stimulates the expression of angiogenic growth factors and angiogenesis, and neutralizes excessive inflammation. Our findings highlight that MnPM may act as an immune costimulatory metalloantibiotic to regulate the cGAS-STING signaling pathway, presenting a promising alternative to antibiotics for orthopaedic biofilm infection treatment and pro-tissue repair.

Abstract Background This study aimed to investigate the potential mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension (ICMT). Methods According to the Pfirrmann grade evaluation classification, 30 human endplate cartilage tissues were divided into the lumbar vertebra fracture (LVF) group and lumbar disc herniation (LDH) group. Then, quantitative reverse transcription polymerase chain reaction, western blot, flow cytometry, hematoxylin–eosin staining, and senescence-associated β-galactosidase staining were performed. The difference in extracellular matrix expression between LVF and LDH endplate cartilage was detected. Second, the effect of ICMT on endplate chondrocytes degeneration was observed. Finally, the key regulatory role of YAP1 in ICMT-induced endplate cartilage degeneration was further verified. Results In degraded human endplate cartilage and tension-induced degraded endplate chondrocytes, the expression of YAP1, COL-2A, and Sox9 was decreased. Conversely, the expression of p53 and p21 was increased. By regulating YAP1 in vivo and in vitro, we can achieve alleviation of ICMT-induced senescence of endplate chondrocytes and effective treatment of disc degeneration. Conclusions ICMT could induce senescence and degeneration of endplate chondrocytes, and ICMT-induced senescence and degeneration of endplate chondrocytes could be alleviated by regulating YAP1 expression.