Objective:To investigate the expression and clinical significance of Jagged1 and Budding uninhibited by benzimidazoles-1 (BUB1) in non-small cell lung cancer (NSCLC).Methods:A total of 108 cases of NSCLC tissues and non-cancerous adjacent lung tissues from Affiliated Hospital of Guangdong Medical University with complete clinicopathological and medical records from August 2018 to May 2022 were collected. The expression levels of Jagged1 and BUB1 in the above tissues were detected by immunohistochemistry, and Chi-square test was used for statistical analysis. The relationship between Jagged1 and BUB1 expression and clinicopathological parameters of NSCLC was analyzed.Results:The positive expression rate of Jagged1 in cancer tissues of NSCLC patients was 81.48% (88/108), and the positive expression rate of Jagged1 in the non-cancerous adjacent lung tissues was 21.30% (23/108) with the difference being statistically significant ( χ2=78.301, P<0.01). The expression level of Jagged1 was significantly correlated with histological differentiation, lymph node metastasis and TNM stage of NSCLC (respectively, χ2=8.062, 5.268, 4.477, P<0.05), but not with gender and age of NSCLC (respectively, χ2=0.238, 0.008, P>0.05). The positive expression rate of BUB1 in cancer tissues of NSCLC patients was 75.00% (81/108), and the positive expression rate of BUB1 in adjacent tissues was 33.33% (36/108) with the difference being statistically significant ( χ2=37.762, P<0.01). The expression level of BUB1 was significantly correlated with lymph node metastasis and TNM stage of NSCLC (respectively, χ2=4.945, 6.008, P<0.05), but not significantly with age, histological differentiation and gender of NSCLC (respectively, χ2=0.013, 0.012, 0.234, P>0.05). Conclusion:Jagged1 and BUB1 are highly expressed in the cancer tissues of NSCLC patients, the abnormally elevated expression levels of Jagged1 and BUB1 are related to the malignant degree and poor prognosis of NSCLC, and are involved in the occurrence and development of NSCLC to a certain extent, which can be used as candidate targets for the diagnosis and treatment of NSCLC.

Objective:To investigate the expression and clinical significance of forkhead box P3 (Foxp3) and membrane-organizing extension spike protein (Moesin) in non-small cell lung cancer (NSCLC).Methods:Foxp3 and Moesin expression in 102 cases of human NSCLC tissues and non-cancerous adjacent lung tissues pathologically confirmed from April 2016 to December 2020 on Affiliated Hospital of Guangdong Medical University was examined by using envision immunohistochemistry. The statistical analysis of Chi-square test was carried out in combination with the relationships between the expression of Foxp3 and Moesin and the clinicopathological features of NSCLC.Results:Foxp3 expression in NSCLC tissues and the non-cancerous adjacent lung tissues was 65.69% (67/102) and 22.55% (23/102) respectively, and differences were statistically significant ( χ2=38.494, P<0.01). The expression of Foxp3 was associated with NSCLC to lymph node metastasis and tumor node metastasis stage (TNM stage) (respectively, χ2=5.890, 4.603, P<0.05). Moesin expression in NSCLC tissues and the non-cancerous adjacent lung tissues was 83.33% (85/102) and 15.69% (16/102) respectively, and differences were statistically significant ( χ2=93.362, P<0.01). The expression of Moesin was associated with NSCLC to histology grade, TNM stage and lymph node metastasis (respectively, χ2=8.483, 4.910, 4.175, P<0.05). Conclusion:Foxp3 and Moesin up-regulation is closely related to disease status of patients with NSCLC, which may be indicators of the occurrence, invasion, metastasis and prognosis of NSCLC.

Objective:To investigate the expression of Yes-associated protein 1 (YAP1) and Erythropoietin producing human hepatocellular receptor A2 (EphA2) in non-small cell lung cancer (NSCLC) and their correlation with clinicopathologic characteristics of NSCLC.Methods:YAP1 and EphA2 expression in 98 cases of human NSCLC tissues and non-cancerous adjacent lung tissues pathologically confirmed from january 2015 to october 2019 in Affiliated Hospital of Guangdong Medical University was examined by envision immunohistochemical method. The χ2 test was carried out for statistical analysis. Results:The positive rate of YAP1 expression was 67.35% (66/98) in NSCLC tissues, higher than 35.71% (35/98) in non-cancerous adjacent lung tissues with significant difference ( t=19.631, P<0.01). The positive rate of EphA2 expression was 72.45% (71/98) in NSCLC tissues, higher than 34.69% (34/98) in non-cancerous adjacent lung tissues with significant difference ( t=28.082, P<0.01). The expression levels of YAP1 were associated with NSCLC to histology grade, TNM stage and lymph node metastasis (respectively, t=10.382, 6.753, 5.315, P<0.05). The expression levels of EphA2 were associated with NSCLC to lymph node metastasis and TNM stage (respectively, t=5.017, 6.607, P<0.05). Conclusion:The positive rate of YAP1 and EphA2 was significantly up-regulated in NSCLC, and YAP1 and EphA2 may be considered as biomarkers for high invasion of NSCLC.

目的 比较EuroSCOREⅡ和阜外瓣膜手术风险评分在心脏瓣膜置换术后预后评估中的效用.方法 回顾分析2013年1月-2017年12月在本院行心脏瓣膜置换手术的376例患者的临床资料,分别使用EuroSCOREⅡ和阜外瓣膜手术风险评分对患者术后预后进行风险评分,评价两种评分系统对心脏瓣膜术后发生死亡及主要并发症的预测效能,采用患者特征曲线下面积(AUC)评价两种模型的区分能力,采用Hosmer-Lemeshow拟合优度检验评价其校正能力.结果 共376例患者施行了瓣膜置换术,共10例患者死亡,实际死亡率2.66％,EuroSCOREⅡ评分和阜外瓣膜手术风险评分预测死亡率分别为(3.5±6.8)％和(3.6±4.2)％;ROC曲线下面积分别为0.872和0.674;Hosmer-Lemeshow检验的P值分别为0.354和0.012;在预测术后主要并发症方面,EuroSCOREⅡ对术后肾功能不全、低心排综合征、机械通气时间长的区分度较好,而阜外瓣膜手术风险评分术后总并发症、术后低心排综合征、术后机械通气时间延长、重症肺炎发生率的区分度较好.结论 两种评分系统对本地区心脏瓣膜置换术后主要并发症的风险预测均有较好的应用价值.

目的 了解保留瓣下结构对二尖瓣狭窄为主型患者瓣膜置换术后早期心功能的影响.方法 121例二尖瓣狭窄为主型患者作瓣膜置换术,其中保留全瓣瓣下结构5例(保留全瓣组),保留后瓣瓣下结构58例(保留后瓣组),二尖瓣及瓣下腱索全切58例(全切组).术后1～6个月行心脏彩色超声多普勒检查.结果 各组未见左心室流入道及流出道梗阻、瓣膜功能障碍,左房舒张期前后径、左室射血分数差异无统计学意义(P＞0.05);保留全瓣组左室舒张期横径、左室短轴缩短率大于保留后瓣组及全切组(P＜0.05).结论 保留瓣下结构对术后早期左室射血分数无明显影响,但全瓣保留者左室短轴缩短率明显提高.

目的 研究保留二尖瓣瓣下结构瓣膜置换术对患者中期心功能的恢复影响,为临床广泛开展该术式提供理论依据.方法 选择2013年1月至2015年8月在高州市人民医院及2009年1月至2015年8月在广东医科大学附属医院因二尖瓣关闭不全为主要病变合并或不合并主动脉瓣疾病行保留二尖瓣瓣下结构二尖瓣膜置换术、二尖瓣及主动脉瓣双瓣置换术患者83例,收集患者出院后6个月以上回手术医院行彩色多普勒超声心动图复查的患者资料.平均随访时间(22.94±16.20)个月.其中保留全瓣瓣下结构9例(组1),保留后瓣瓣下结构42例(组2),二尖瓣及瓣下腱索全切32例(组3).各组均应用双叶机械瓣.测定患者左房舒张期末前后径、左室舒张期末横径、左室射血分数、左室短轴缩短率;同时测定左室流入道及流出道、各瓣膜功能、瓣周情况.结果 组1、组3、组2患者左房舒张期末前后径分别为(38.11±7.27)mm、(39.39±7.13)mm、(42.47±8.82)mm,左室舒张末横径分别为(44.00±6.18)mm,(44.54±7.01)mm,(45.93±6.69)mm,左室射血分数分别为(59.00±3.87)mm、(58.70±8.16)mm、(57.86±9.72)mm,左室短轴缩短率分别为(31.00±4.12)％、(30.58±6.70)％、(28.25±10.43)％,以上各项指标比较差异均无统计学意义(P>0.05);所有患者无左室流入道及流出道梗阻,无瓣周漏,人工机械瓣膜启闭良好.结论 对二尖瓣关闭不全为主病变或同时合并主动脉瓣病变行二尖瓣置换或双瓣膜置换术患者进行中期随访,虽然各项指标无明显差异,但数据表明保留全瓣及瓣下结构较保留后瓣及瓣下结构及全切对中期心功能恢复帮助更明显.术中应用双叶机械瓣,术中精心操作,术后不会导致左室流入道及流出道梗阻、瓣膜功能障碍.

目的 研究聚乙烯醇辅助治疗中央型肺癌的疗效和安全性.方法 纳入70例中央型肺癌患者作为研究对象,分为观察组和对照组,每组各35例.观察组行经微导管灌注化学治疗再进行聚乙烯醇栓塞治疗,对照组行常规支气管动脉内灌注化学治疗.比较2组近期疗效和安全性,随访记录2组患者远期预后.结果 2组患者介入治疗均获得成功,观察组治疗后近期总体疗效优于对照组(P<0.05),组间不良反应发生率比较差异无统计学意义(P>0.05).治疗结束后5年,观察组的生存率为23％(8/35),生存时间(22.8±7.7)月,无进展生存率11％(4/35),无进展生存时间(18.3±5.7)月;对照组的生存率为17％(6/35),生存时间(19.0±8.1)月,无进展生存率9％(3/35),无进展生存时间(15.3±6.2)月.2组总生存率和无进展生存率比较差异均无统计学意义(P均>0.05).结论 聚乙烯醇经微导管栓塞治疗中央型肺癌近期疗效好,安全性高,但其对改善患者远期生存率的效果有限.

Objective To investigate the clinical effect of selective bronchial artery infusion chemotherapy for central squamous cell carcinoma of the lung.Methods 93 cases of central lung squamous cell carcinoma patients who were treated in our hospital from February 2015 to February 2017 were enrolled in the study.They were divided into the observation group (n =45) and the control group (n =48) according to the patients'final treatment.The observation group was treated with bronchial artery perfusion chemotherapy,and the control group was treated with systemic venous chemotherapy.The short-term effect and the improvement of lung function in the two groups were observed,and the prognosis of the patients was followed up.Results The short-term effect of the observation group was better than that of the control group (P ＜0.05),and the total effective rate of the observation group was 95.56％;The forced vital capacity (FVC)and forced expiratory volume in the first second (FEV,) of the observation group were (75.39 ± 6.88)％and (78.19 ± 7.79)％,respectively,which were significantly higher than those in the control group (P ＜0.05).The reoperation ratio of the observation group was 44.44％ after chemotherapy,which was significantly higher than that of the control group,with statistically significant difference (P ＜ 0.05).The median total survival time of the observation group was 17 months,which was significantly higher than that of the control group,with statistically significant difference (P ＜ 0.05).The incidence of adverse reaction in the observation group was 6.67％,which was significantly lower than that in the control group,with statistically significant difference (P ＜ 0.05).Conclusions Selective bronchial artery infusion chemotherapy for central lung cancer is effective and worthy of clinical application.

Objective To investigate the changes and clinical significance of serum tumor markers in patients with non-small cell lung cancer before and after gefitinib targeted therapy.Methods 80 cases of non-small cell lung cancer patients in our hospital from June 2015 to May 2017 were divided into control group and observation group randomly,40 cases in each group.The control group were treated with docetaxel conventional chemotherapy,and the observation group were treated with gefitinib targeted therapy.The clinical treatment effect,changes of serum tumor markers cancer antigen125 (CA125),carcinoembryonic antigen (CEA),neuron specific enolase (NSE) and adverse reactions were observed and compared between the two groups before and after treatment.Results The effective rate and disease control rate of the observation group were higher than that in the control group,with statistically significant difference (P ＜ 0.05).The levels of serum tumor markers CA125,CEA and NSE in the control group and the observation group before treatment were not significantly different (P ＞ 0.05).After 1 months of treatment,the levels of serum tumor markers CA125,CEA and NSE in the two groups were all decreased,and the level of serum tumor markers,CA125,CEA and NSE in the observation group were lower than those in the control group,with statistically significant difference.The incidence of adverse reactions in the observation group was lower than that in the control group (P ＜ 0.05),with statistically significant difference.Conclusions Gefitinib is effective in the treatment of non-small cell lung cancer.It reduces the level of serum tumor markers CA125,CEA,NSE,and reduces postoperative adverse reactions.It is worthy of clinical application.

目的 了解及评价不同二尖瓣置换术式对双瓣置换术后患者中远期左室收缩功能变化的影响.方法 回顾分析二尖瓣主动脉瓣双瓣置换术后患者,术后出院6个月以上回院复查超声心动图的199例患者的临床资料.根据术中二尖瓣瓣下结构保留程度的不同分为3组:A组20例患者为保留全部瓣下结构;B组95例保留后瓣叶瓣下结构;C组84例全部切除二尖瓣前后瓣叶下结构.平均随访时间(21.61±14.72)月.对比分析3组患者术后超声心动图检查结果.结果 术后中期A组的左心室短轴缩短率高于其余两组,差异有统计学意义(P<0.01),但3组患者在左房前后径、左室前后径和左心室射血分数方面差异均无统计学意义(P>0.05).结论 双瓣置换术中,完全保留二尖瓣瓣下结构比仅保留后瓣或不保留瓣下结构更有利于中期左心室收缩功能的恢复.

目的 探讨下肺叶切除术后应用腹带以促进患者快速康复的可行性.方法 2015年3月至2016年5月在本院行下肺叶切除术的患者50例,随机分为两组,观察组26例,对照组24例.对照组术后接受加强呼吸功能锻炼、保持引流管通畅等传统方法治疗,观察组加用腹带束腹,余治疗措施相同.比较两组患者术后胸腔积液引流量、拔胸管时间等,观察两组术后胸内残腔情况.结果 观察组术后胸腔积液引流量、拔管时间均明显小于对照组(P<0.05).结论 下肺叶切除术后应用腹带可减少胸腔积液,缩短拔管时间而有助于术后快速康复.

目的 比较钢丝、可吸收髓内钉、记忆合金环抱器、条形钢板四种肋骨内固定材料在肋骨骨折内固定中的差异.方法 近4年来我科共实施肋骨骨折内固定术83例,其中钢丝固定者11例,可吸收髓内钉固定者34例,记忆合金环抱器固定者25例,条形钢板固定者13例.比较四种固定方式在手术费用,手术时间,胸廓畸形矫正程度,断端复位情况,牢固程度及疼痛程度方面的差异.结果 钢丝肋骨固定术操作简单,手术时间短,手术费用低廉,断端复位情况及牢固程度较差,且不可吸收.可吸收髓内钉肋骨固定手术费用较高,断端复位情况及牢固程度较差,但可吸收.记忆合金环抱器操作复杂,手术时间长,费用较高,断端复位情况及牢固程度好.条形钢板手术操作简单,断端复位情况及牢固程度可,术后无肋间神经卡痛现象,但不可吸收,放射线下表现明显.结论 四种材料的肋骨固定各有其优缺点,临床上应结合固定材料的特点,病情以及患者经济能力综合考虑应用.

Objective To investigate the expression of B7-H4 and cell division cycle 42(Cdc42)in non-small cell lung carcinoma(NSCLC),and their relation to the clinicopathological features.Methods Envision immunohistochemical method were used to assess the expression of B7-H4 and Cdc42 in 92 cases of human NSCLC tissues and non-cancerous adjacent lung tissues.Results The expression rate of B7-H4 in breast carcinoma tissues was higher than that of non-cancerous adjacent breast tissues(44.57％vs.8.70％,P＜0.01),the expressions of B7-H4 were related NSCLC to TNM stage and lymph node metastasis(P＜0.05);the expression rate of Cdc42 in breast carcinoma tissues was higher than that of non-cancerous adjacent breast tissues(56.52％vs.4.35％,P＜0.01);there was a close relation between the expression of Cdc42 and histology grade,histology grade and TNM stage(P＜0.05).Conclusion B7-H4 and Cdc42 may be participate in the development and progression of NSCLC.

Objective To detect the serum receptor-binding cancer antigen expressed on SiSo cells (RCAS1) in patients with esophageal carcinoma,and to investigate the effects of operation on it.Methods Enzyme linked immunosorbent assay (ELISA) was used to detect the level of serum RCAS1 in 64 cases of esophageal carcinoma and 30 cases of normal controls,and the serum RCAS1 leves were compared in 40 cases of esophageal carcinoma before and after operation.Results The levels of serum RCAS1 were elevated in all subgroups of patients with esophageal carcinoma [(38.89 ±13.00) U/ml] compared to the controls [(15.59 ± 4.41) U/ml,P ＜ 0.01].Serum RCAS1 was not correlated with sex,age,and histological grade of patients with esophageal carcinoma,but with clinical stage.The serum RCAS1 level in stage Ⅳ [(61.21 ±5.32) U/ml] was higher than that in comparison with stage Ⅲ [(47.33 ±3.72) U/ml,P ＜ 0.01].Serum RACS1 level in stage Ⅲ was higher than that in stage Ⅱ [(36.28 ± 4.81) U/ml,P ＜0.01],that in stage Ⅱ higher than that in stage Ⅰ [(22.47 ±5.77) U/ml,P ＜0.01],and that in stage Ⅰ higher than that in normal controls (P ＜ 0.05).The serum RCAS1 levels were remarkably reduced in patients with esophageal carcinoma after operation (P ＜ 0.01).Conclusion The serum RCAS1 is closely correlated with the malignant degree and prognosis in esophageal carcinoma.

Objective To investigate the changes and its clinical value of serum Thymidine Kinase 1 (TK1) in patients with non-small cell lung cancer(NSCLC).Methods Sensitive chemiluminescence dot-blot assay were available to detect the level of serum TK 1 in 64 cases of patients with NSCLC and 31 cases of normal control subject, compared with 44 patients with NSCLC pre-and post-operation.Results Serum TK1 was correlated with clinical stage , and not with pathologic types , age and sex of patients with NSCLC , the level of serum TK1 were elevated in all subgroups of patients with NSCLC compared to the controls (P<0.01), the serum TK1 level in stage Ⅳwas higher than that in stage Ⅲ, in stage Ⅲ it was higher than that in stage Ⅱ, and in stage Ⅱit was higher than that in stageⅠ(P<0.01~0.05), the serum TK1 levels were remarkably reduced in patients with NSCLC after operation(P<0.01).Conclusions This study demonstrates that the expression of serum TK1 have a relationship with clinical pathological feature of patients with NSCLC , it may be act as one index to assess the disease status and the prognosis of NSCLC .

Objective To explore the serum expression of insulin like growth factor-1 (IGF-1) in patients with non-small cell lung cancer (NSCLC) and its clinical significance in the evaluation of treatment outcome.Methods The level of serum IGF-1 was measured by radioimmunoassay (RIA) in 32 cases of normal control subjects and 63 cases of NSCLC,and compared in 44 patients with NSCLC before and after operation.Results The serum IGF-1 was correlated with clinical stage of patients with NSCLC,but not with sex,age and pathologic types.The levels of serum IGF-1 were elevated in all subgroups of patients with NSCLC [(258.22 ± 52.49) μg/L] compared to the controls [(155.04 ± 21.37) μg/L] (P ＜0.01).The serum IGF-1 level in stage Ⅳ [(322.08 ± 27.46) μg/L] was higher than that in stage Ⅲ [(280.42 ±26.12) μg/L] (P＜0.01),that in stage Ⅲ was higher than that in stage Ⅱ [(241.59 ± 22.84) μg/L] (P ＜ 0.05),that in stage Ⅱ was higher than in stage Ⅰ [(195.20 ± 19.27) μg/L(P ＜0.01),and that in stage Ⅰ was higher than that in normal control subjects (P ＜0.01).The level serum IGF-1 was obviously reduced in patients with NSCLC after operation (P ＜ 0.01).Conclusion Detection of serum IGF-1 may be a useful reference index to state the illness and evaluate the therapeutic effect of NSCLC.

Objective To investigate the related risk factors of low cardiac output syndrome after heart valve replacement in elderly patients.Patients were divided into case group (n =45) and control group (n =234) according to whether they suffered from low cardiac output syndrome after surgery or not.We collected clinical information among the patients and utilized Univariate analysis,chi-square test and ttest to analyze data,and then used two classification logistic regression analysis to analyze the risk factors which having significant difference in the univariate analysis.Results Univariate analysis showed that preoperative mean arterial pressure,renal insufficiency situation,intraoperative aortic clamping time and the volume of blood loss,postoperative electrolyte imbalance,cardiac arrhythmias and multiple organ failure all had statistically significant differences between two groups (P ＜ 0.05).Two classification logistic regression analysis showed that blood loss/total blood volume [odds ratio(OR) =0.32,P ＜ 0.05],aortic clamp time (OR =1.85,P ＜ 0.05),electrolyte disorders (OR =1.43,P ＜ 0.05),arrhythmia (OR =1.61,P ＜ 0.05) are all the risk factors for low cardiac output syndrome after heart valve replacement surgery.Conclusion Preoperative mean arterial blood pressure,intraoperative aortic clamping time,the amount of blood loss and postoperative electrolyte imbalance,cardiac arrhythmias,and multiple organ failure are all the factors which can affect elderly patients with heart valve replacement surgery emerged low cardiac output syndrome or not,among which aortic clamping time,blood loss,postoperative cardiac arrhythmias and electrolyte imbalance are the important risk factors.

Background: Natural rubber latex glove use is widespread in mainland China, but the prevalence and risk factors for latex glove allergy among clinical nurses have previously been unreported.Methods: A questionnaire was used to collect information on latex glove-related allergy among clinical nursing staff in 35 hospitals of eight provinces in the southern, central southern, and northern regions of China, and the risk factors were calculated with logistic regression analysis. Some subjects with glove dermatitis were patch tested with a modified European standard series of allergens.Results: Among 8485 female nurses in eight provinces of China, overall prevalence of latex glove allergy was 8.8%. Of 743 symptomatic nurses, 573 (77.1%) and 475 (63.9%) reported symptoms suggestive of glove dermatitis and type I latex allergy, respectively. Of 69 randomly selected subjects with glove dermatitis, 18 (26.1%) had a positive patch to rubber additives. Employment seniority, positive family and personal history of allergic diseases, and longer extent of time spent in a single hospital room were associated with latex allergy, while using >5 pairs of gloves per working day may be a protective factor.Conclusion: Chinese nurses are at high risk for latex sensitization. Nurses who develop latex-related symptoms after exposure to latex gloves should undergo screening tests for latex allergy. Low-protein, powder-free natural rubber latex gloves, or latex-free gloves should be widely adopted in China, along with other preventive measures.

Objective To investigate the expression of tumor suppressor in lung cancer 1 (TSLC1) and microvessel density (MVD) marked with CD105 in esophageal carcinoma.Methods Envision immunohistochemical method was used to assess the expression of TSLC1 and MVD marked with CD105 in 45 cases of esophageal carcinoma tissues and non-cancerous adjacent esophageal tissues.Results The expression rate of TSLC1 was 42.22％ (19/45) in esophageal carcinoma tissues,and 91.11％ (41/45) in non-cancerous adjacent esophageal tissues (P ＜ 0.01).The expression of MVD marked with CD105 was 38.25 ± 4.37 in esophageal carcinoma tissues,and 11.39 ± 1.52 in non-cancerous adjacent esophageal tissues (P ＜ 0.01).The expression of TSLC1 was correlated to TNM stage and lymph node metastasis (P ＜ 0.05).There was a close correlation between the expression of MVD marked with CD105 and histologic grade,TNM stage and lymph node metastasis (P ＜ 0.05).Conclusion TSLC1 and CD105 may play a significant role in the onset and progression of esophageal carcinoma.