为了解加味小柴胡散对鹌鹑生长性能、血液生化指标及免疫功能的影响,选取8日龄鹌鹑300只,随机分为5组,A组为对照组,B组为添加抗菌药组,C组、D组和E组分别在基础日粮中添加0.5％、1％和2％的加味小柴胡散.结果表明,与抗菌药B组相比,加味小柴胡散可显著促进鹌鹑日增重和采食量(P＜0.05),耗料增重比显著降低(P＜0.05).试验组的免疫器官指数显著高于抗菌药B组(P＜0.05),血清总蛋白(TP)、白蛋白(ALB)显著高于抗菌药B组(P＜0.05),加味小柴胡散可显著提高肠绒毛长度,降低隐窝深度和肠壁厚度(P＜0.05).表明加味小柴胡散可明显促进鹌鹑的生长发育和免疫器官的发育,且以1％添加剂量效果最佳.

本试验从25个绿壳蛋鸡场进行样本的采集,每个鸡场随机采集24枚鸡蛋,总计采集600份样本,通过HA-HI试验方法进行新城疫和禽流感抗体的检测,结果显示,所检测的25个养殖场中,新城疫(ND)的抗体效价合格率为80％(20/25);禽流感(AI)H9亚型的抗体效价合格率为68％(16/25),H7亚型的抗体效价合格率为52％(13/25),H5Re-11和H5Re-12亚型的抗体效价合格率都较低,说明本地区的绿壳蛋鸡新城疫的免疫情况较好,而禽流感的免疫情况不理想.

肾型传染性支气管炎病毒( IBV )因血清型多、易变异,相互间的交叉保护率低,致使该病难以控制,特别是发病后主要引起肾脏的肿大,西药治疗效果不佳. 笔者通过对1例肾型传染性支气管炎并发大肠杆菌病的诊断并采用中西药进行治疗,收到良好的治疗效果. 现将诊治情况介绍如下,供参考.

肾型传染性支气管炎病毒因血清型多、易变异,相互间的交叉保护率低,而使其难以控制,特别是发病后主要引起肾脏的肿大,西药治疗效果并不理想,笔者通过采用中药对麻鸡肾型传染性支气管炎进行治疗,达到了良好的临床治疗效果. 1 临床症状 2017年12月吉林市金珠乡某养殖户,饲养的2 000羽9周龄麻鸡发病,临床表现呼吸道症状,排黄白色稀粪、肿眼、精神沉郁为主并出现死亡的传染病,而且死亡数量逐渐增加.

猪瘟（Classical Swine Fever,CSF）亦称为"烂肠瘟",其由猪瘟病毒引发在野猪和家猪身上的一种接触性、热性和急性传染病,主要特征是伴有肠道特征,每年给养猪业造成严重经济损失[1]。随着养猪业合理科学的免疫程序的制定和运行,猪瘟发病情况也发生了变化,呈现散发、发病年龄提前、混感、非典型症状或者隐性感染等特征。笔者曾接诊到一起猪瘟和葡萄球菌混合感染的病例,该病例来自于

吉林省长春市双阳区某梅花鹿场发生以鹿蹄冠部和体表皮肤化脓、坏死和跛行为特征的疾病,根据临床检查、病理变化和实验室检验,确诊为鹿坏死杆菌病,通过采取综合防治措施控制了病情的发展,效果较好. 现将诊治情况报道如下.

从猪萎缩性鼻炎的病猪鼻腔内分离和纯化病原菌,通过药敏试验筛选出对猪萎缩性鼻炎病原菌敏感的药物,并通过鼻腔喷雾给药、肌肉注射给药和拌料给药的方法防治猪萎缩性鼻炎.结果表明:通过鼻腔喷雾给药的猪平均日增重显著高于拌料组和肌肉注射组(P<0.01).说明鼻腔给药,药物可直接作用于患部,疗效迅速,方便快捷,是防治猪萎缩性鼻炎的最佳给药途径.

鸡毒支原体(Mycoplasma gallisepticum,MG)会引发鸡的慢性呼吸道疾病(chronic respiratory disease,CRD).本研究从吉林经济技术开发区鸡场采集的疑似支原体感染病料中分离、培养获得一菌株,经菌落形态观察、菌落红细胞吸附试验、生化特性测定及血清学鉴定等试验,证明该分离株为鸡毒支原体(MG),并命名为JL株.

Materials from pigs with diarrhea were collected from development zone,Jilin surrounding three large-scale pig farms,cultural characteristics and bacterial form of observation,biochemical identification combined with clinical symptoms and epidemiological characteristics were explored.And sensitivity tests were used in 12 kinds of drugs.The results showed salmonella against amikacin and kanamycin,highly sensitive,against tetracycline and trimethoprim sulfa six pyrimidine and levofloxacin,moderately sensitive;against penicillin,streptomycin,tetracycline,soil doxycycline,erythromycin,enrofloxacin,norfloxacin,resistance.

目前对肉鸡腹水综合征尚无理想的治疗方法,主要采取"预防为主,防治结合"的方法.近年来,随着肉鸡产业的不断发展,肉鸡腹水综合征发生率呈上升势态,已成为危害肉鸡养殖业的三大疾病之一.本试验采取三种不同的中西医结合的治疗方案对肉鸡腹水综合征进行治疗,试图找到治疗效果比较好的方案.

从开发区周边一些肉仔鸡养殖场采集腹泻病死鸡的病料,经培养特性和细菌形态观察,生化鉴定,结合临床症状及流行病学特点,最终确定主要病原菌有沙门氏杆菌和多杀性巴氏杆菌。并用12种药物做了药敏试验,结果多杀性巴氏杆菌对恩诺沙星、阿米卡星、左氧氟沙星、庆大霉素高度敏感;对青霉素、链霉素、四环素、土霉素、磺胺六甲氧嘧啶中度敏感;对氟哌酸、卡那霉素、红霉素耐药。而沙门氏杆菌对阿米卡星、卡那霉素高度敏感;对四环素、磺胺六甲氧嘧啶、左氧氟沙星中度敏感;对青霉素、链霉素、四环素、土霉素、红霉素、恩诺沙星、氟哌酸耐药。

吉林省某种羊场2009年用仔猪"551"饲料饲喂波尔羔羊,饲喂5个多月后,先后有16只羔羊发病,发病率为13.2%.临床可见可视黏膜苍白黄染和排血红蛋白尿.剖检眼观病变血液稀薄,肝肿大呈土黄色;光镜下,肝细胞变性坏死,结缔组织纤维化,肾小管上皮细胞变性坏死;电镜下,肝细胞核变形,内质网扩张,溶酶体内铜颗粒过载,毛细胆管微绒毛肿胀或断裂.血清铜含量高于正常值,天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)等酶活性均升高.仔猪"551"饲料检测,铜含量为200 mg/kg,用此饲料进行饲喂试验,结果受试羊于153d内全部发病死亡,临床症状和病理变化与自然病死羔羊基本相同.最终确诊为羔羊慢性铜中毒.对病羔羊实施治疗,治愈7只,死亡9只.

<正>吉林市某鸡场的肉仔鸡在30日龄时发病,通过对病鸡的剖检,以及血凝试验、血凝抑制试验,诊断该鸡场病鸡所患为非典型新城疫。用3种不同方剂对发病的肉仔鸡进行了治疗。诊疗过程报道如下。

In order to determine the technology of CO2 supercritical fluid extraction of the essential oil from Artemisia argyi and the antibacterial activity of the essential oil,Used the technology of CO2 supercritical fluid extraction,filter paper diffusion method to determine inhibitory effect.The results showed that the optimizing technology of CO2 supercritical fluid extraction was using fresh raw materials of Artemisia argyi without entertainer,the pressure 35Mpa,temperature 45 ℃,extraction time 90min.The essential oil from Artemisia argyi had significant inhibitory effect on escherichia coli and staphylococcus aureus,and the effect increased with the concentration.Indicated that the essential oil from Artemisia argyi extracted by the optimizing technology of CO2 supercritical fluid extraction had antibacterial effects.

<正>从吉林市部分养猪场发病死亡病猪采集了病料,经细菌形态和培养特性观察、染色镜检、生化试验,结合临床症状及流行病学特点,最终确定病原体为猪多杀性巴氏杆菌。并用9种药物做了药敏试验,

The attenuated GPMV F gene was cloned into the eukaryotic expression vector of pCI-neo and the pCI-rf plasmid was obtained.The pCI-rf plasmid was transfected into BHK cells and the fusion protein was detected through Western blotting successfully.The 7 to 9-day-old chicken was immunized intramuscularly with pCI-rf and pCI-f separately.The antibody level was detected by indirect ELISA and the cell immunity was detected by lymphocyte proliferation assay and specific lysis activity of CTL test.The immunized chicken was challeged finally.The result showed that both of pCI-rf and pCI-f induced cell immunity and humoral immunity responses and the difference of immune effect was not obvious comparing with Lasota vaccine.The PCR result showed that F gene fragment was not detected from the genome of immunized chicken tissues.

鸭瘟又称鸭病毒性肠炎,是由鸭瘟病毒引起的一种高死亡率、急性败血性传染病.通过消化道、眼结膜、呼吸道、伤口及接触等途径而感染.本病的主要特征是头颈肿大、高热、流泪、下痢、粪便呈灰绿色,两腿麻痹无力.发病后期体温降低至正常体温以下,最后衰竭死亡.笔者将一例绿头野鸭患鸭瘟致死诊断情况报告如下.

本试验应用ELISA法对吉林地区8个PRRS未免疫猪场和9个PRRS免疫猪场的后备母猪、生产母猪和5～10周龄的断奶仔猪三个猪群进行了PRRSV抗体检测.结果表明:吉林地区8个PRRS未免疫猪场均受到PRRSV感染,猪场抗体阳性率为100%,猪群抗体阳性率在50%-75%之间,平均阳性率为62.26%;吉林地区9个PRRS免疫猪场猪群PRRSV抗体阳性率在70%-100%之间,平均阳性率为87.78%.PRRS未免疫猪场与PRRS免疫猪场猪群抗体平均阳性率相比,差异极显著(P＜0.01).

Using red raspberry of fullness as raw materials,the enzyme extract juice,and ultrafiltration technology were applied to the raspberry juice in the process of clarification,after clarification and flavor deployment,a kind of clarification of raspberry drink was developed. It remained the original fruit relish,color and nutrients. It was delicious in sour and sweet and rich in nutrient.It had of better stability.

本试验应用ELISA法对吉林地区8个猪繁殖与呼吸综合征(PRRS)未免疫猪场和9个PRRS免疫猪场的后备母猪、生产母猪和5～10周龄的断奶仔猪3个猪群进行了猪繁殖与呼吸综合征病毒(PRRSV)抗体检测,结果表明:吉林地区8个PRRS未免疫猪场均受有PRRSV感染,猪场抗体阳性率为100%,猪群抗体阳性率在50%～75%之间,平均阳性率为62.26%;吉林地区9个PRRS免疫猪场猪群PRRSV抗体阳性率在70%～100.00%之间,平均阳性率为87.78%.PRRS未免疫猪场与PRRS免疫猪场猪群抗体平均阳性率相比,差异极显著(P<0.01).