目的建立高效液相色谱法测定前列腺素E1的含量方法.方法选用Shimadzu C18柱,5μm,4.6mm×150mm,0.02mol/L磷酸二氢钾(pH 4.9)-乙腈(3:2)为流动相,检测波长214nm,流速1.0ml/min,柱温:室温,进样量:10μl.结果在100～350μg/ml-1浓度范围内,线性关系良好,回归方程Y=0.4858x-0.0042,r=0.9992.结论本法测定前列腺素E1的含量,结果准确.

Objective The treatise reported the PSA levels in the sera of 526 asymptomatic men(over 50 years old),16 men(age between 40-49) and 101 men(younger than 40years,control group) were determined.Methods Ninety-three men whose serum PSA levels were highere than 4.0?ng/ml were selected for biopsy.Tissues pathology analysis as well as PSA expression of prostatic tissues derived from biopsy were analyzed by methods of immunohistochemistry in biopsied men.Results Prostatic hyperplasia was 81.08% in 40 case sample,prostatic cancer was 0.95%.Conclusions The incidence rates of analysis benign prostatic hyperplasia was more than 60% and that of prostatic cancer was about 1% in men over 50 years old.

Objective To study the effect of AR and sex hormones as well as change with age on prostatic disease. Methods AR was expressed by dot blot.Sex hormones contents(testosterone?LH?cortisone?estradiol)were determined by radioimmune method. Results A progressive declines of serum testosterone levels and increase of serum LH levels with age were found.Serum cortisol contents were found increased with age.Serum estradiol levels in surveyed men over 40 were higher than that of the men in control group.The estradiol concentration did not change more in the men over 50.Dot blot:AR hybrid signal of prostate cancer was stronger than that prostate hyperplasia and prostate tissues. Conclusion Testosterone levels was negatively correlated with age(r=-0.45,P0.000?1),Chang of LH was opposite.Cortisol contents was positively correlated with age.Analysis of dot blot showed that ARmRNA expression in CaP tissues was higher than that of controls,and that ARmRNA expression in BPH tissues was no difference compared with controls.

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的　pSFJ1 6与pSFJ38均是以牛痘病毒包涵体 （ATI）启动子和分别串联的 1 6个和 38个p7 5突变型早期启动子为基本构件组成的复合型启动子 （ATI-p7 5）、痘苗病毒复制非必需区血凝素 （HA）基因为侧翼的痘苗病毒表达载体。分别插入编码艾滋病病毒 （HIV - 1 ）外膜蛋白env、核心蛋白gag与编码干扰素 （IFNα - 2b）基因 ,观察其表达产物诱导的机体内产生抗体效价的变化规律。方法　利用分子克隆技术构建重组质粒 ,经脂质体转染与野生型痘苗病毒在Cos- 7细胞内同源重组 ,筛选、纯化、获得重组痘苗病毒vJ1 6env/IFNα - 2b和vJ38gag/IFNα - 2b。免疫小鼠后 ,经间接ELISA分折免疫小鼠血清抗体的OD4 90 值变化。结果　免疫小鼠后体内抗体的OD4 90 值 ,实验组与阴性对照组平均数有显著性差异 （P &lt;0 0 5） ,实验组与阳性对照组之间无显著性差异 （P &gt;0 0 5）。结论　重组痘苗病毒vJ1 6env/IFNα - 2b和vJ38gag/IFNα- 2b免疫小鼠后可诱导机体产生高滴度的抗HIV - 1env、gag与IFNα - 2b巨蛋白抗原的抗体 ,蛋白稀释度与血清抗体OD4 90 值呈负相关</span>

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的　以 7所医院近 5年来前列腺癌手术与组织活检为样本 ,研究TGFβ1、EGF、P5 3及PCNA与前列腺癌发生发展的内在规律。方法　通过免疫组化的方法对 3 6例患者进行定位、定性分析。结果　TGFβ1、EGF在前列腺癌组织中其腺上皮的癌细胞的胞浆、胞核与周围基质呈阳性达 80 %以上 ,P5 3及PCNA使癌细胞的胞核染色呈强阳性 ,胞浆染色弱阳性约占 60 %。结论　TGFβ1、EGF是基质和上皮相互作用信号传导通路的重要组成部分 ,与前列腺癌的病因及异常生长具有相关性。P5 3与PCNA阳性染色 ,说明P5 3蛋白基因发生突变与癌细胞增殖活跃。</span>

目的探讨前列腺增生与前列腺癌同时伴有前列腺特异性抗原(PSA)增高患者的组织病理变化特点.方法以PSA为标记物,在626人中进行前列腺疾病普查.通过手术或经会阴四点穿刺取活体组织观察其形态学.前列腺组织标本经10%福尔马林固定,石蜡包埋, HE与Masson染色.结果 37例活检标本中,30例伴有不同程度的前列腺增生,5例为前列腺癌.结论 50岁以上人群中前列腺增生的发病率占81.08%,前列腺癌的发病率＞1%.

目的 本实验选择的真核表达载体 pJ16 ,是以痘苗病毒复制非必需区血凝素 （HA）基因为侧翼 ,以牛痘病毒包涵体 （ATI）启动子和串联的 p7 5突变型早期启动子为基本构件的痘苗病毒表达载体。插入编码AIDS病毒 （HIV -1）外膜蛋白env与编码干扰素IFNα - 2b基因。方法 经与野生型痘苗病毒在细胞内同源重组 ,挑选HA-斑。获得重组痘苗病毒vJ16env/IFNα - 2b。经免疫荧光、Dotblot、SDS -PAGE、Westernblot和免疫小鼠后血清抗体IgGOD值检测。结果 vJ16en/IFNα - 2b能表达Env -IFNα - 2b融合蛋白 ,分子量约 10 0kDa。免疫小鼠实验 ,血清抗体IgGOD值组间均数比较 （P ＜0 0 0 1） ,含IFNα- 2b与vJ6env组比较 （P ＞0 0 5 ） ,但有增高趋势。结论 重组病毒表达的Env -IFN融合蛋白具有良好的反应原性和免疫原性。