Objective:To study the expression and clinical significance of MUC1,hTERT mRNA and DPC4 in pancreatic carcinoma.Methods:Our study applied an immunohistochemical SABC assay to discover the expression of MUC1 mRNA,hTERT mRNA and DPC4 mRNA in the 8 cases of normal pancreatic tissue and 47 cases of pancreatic cancer tissue in paraffin blocks.After that,we analyzed whether or not this expression was in relation to the indicators of the patient's gender,age,tumor size,tumor location,the pathological differentiation or TNM.Results:The positive expression rate of MUC1 mRNA,hTERT mRNA and DPC4 mRNA in eight cases of normal pancreatic tissue were 25.0%,12.5%,100%.The positive expression rate in 47 cases of pancreatic cancer tissue were 78.72%,76.60% and 55.32%.It showed significant difference in the expression rate between two groups(P0.05).In human pancreatic cancer tissues,both the MUC1 mRNA expression and hTERT mRNA expression showed significant differences between patients suffered lymphatic metastasis and patients who were not found lymphatic metastasis(P0.05).Moreover,hTERT mRNA was also in relation to the degree of tissue differentiation(P0.05).However,the positive expression rate of DPC4 mRNA did not show significant difference in any clinical feature in pancreatic cancer tissues(P0.05).It did not show statistical correlations in the expressions of MUC1 mRNA,hTERT mRNA and DPC4 mRNA in pancreatic cancer tissues(P0.05).Conclusion:In pancreatic cancer tissues,the expression of MUC1 mRNA,hTERT mRNA were increased,while the expression of DPC4 mRNA was reduced.All of the three genes play an important role in the occurrence,growth and metastasisof pancreatic cancer.MUC1 mRNA,hTERT mRNA and DPC4 mRNA combined detection contribute to the early diagnosis of pancreatic cancer.

Objective To discuss the epidemiological feature,the pathogenesis.the diagnosis and the treatment of retroperitoneal tumor in China from 1998 to 2007.Methods The document on retroperitoneal tumor was reviewed and analyzed for the epidemiological feature and the experiences on diagnosis and treatment from 1998 to 2007 during 10 years.Results 10 782 patients were reported in China during 10years.The ratio between male and female was approximately 1.30:1.The mean age of the patients was 41.8years.The 48.4%of the patients distributed in north China.The abdominal mass was the main clinical manifestation in 44.3%of the patients and 68.3%of the patients was malignant retroperitoneal tumor,ultrasound and CT were major diagnostic measure and surgical management was the most important therapeutic method.Conclusions Retroperitoneal tumor was more common in north China.It was difficult to early make diagnose before operation.Surgical removal was the most effective method for the treatment of the patients with retroperitoneal tumor.

目的 分析中国近20年侵袭性纤维瘤的流行病学特征及诊疗经验.方法 通过维普数据库和中国知网等多家中文数据库,检索中国近20年有关侵袭性纤维瘤的文章,总结分析侵袭性纤维瘤的流行病学特征和诊治经验.结果 中国近20年共报道侵袭性纤维瘤969例,男女比例约为1∶1.71,平均年龄29岁,高发于10 ～40岁；42.31％分布于华北地区,33.23％分布于华东地区；首发症状中81.23％为无痛或痛性肿块,13.86％为肿瘤压迫引起功能障碍或疼痛；术前诊断为侵袭性纤维瘤仅86例(8.88％),27例于术前行穿刺活检,确诊为侵袭性纤维瘤15例(55.56％)；655例施行根治性手术,95例行姑息性手术,52例术后行放疗,15例于明确诊断后放弃治疗；766例报告随访结果,其中于2个月～8a内复发188例,复发率24.54％.结论 侵 袭性纤维瘤主要分布在华北、华东地区,发病率较低；以无痛或者痛性肿块为主要的临床表现形式,无特异性,术前诊断较困难；治疗以肿瘤一期根治性切除为主,放疗是有效的辅助方法.

Objective To explore the epidemiological feature,pathogenesis, diagnosis and treatment on retroperitoneal fibrosis(RPF) in China from 1998 to 2007. Methods The epidemiological feature and the experiences of diagnosis and treatment on RPF was reviewed and analyzed from the clinical materials on documents during 10 years.Results 652 patients with RPF were reported in China during 10 years,of which the ratio of male patients to female patients was approximately 1.81:1.The mean age of these patients was 48.6 years. 31.7﹪ of these patients was distributed mainly over North Part of China.The back or flank pain and abdominal pain was the main clinical manifestation in 70.7﹪ of these patients and surgical resection was the main therapeutic method. Conclusion The patients with RPF was distributed mainly over East and North Part of China. Preoperative diagnosis was more difficult. Pathological diagnose is the golden standard for diagnose of RPF. Surgical therapy and adhesions release were the effective methods for the treatment of this disease.

AIM:To analyze the clinical significance of expression of human telomerase reverse transcriptase (hTERT) and mucin 4 (MUC4) mRNAs in peripheral blood of patients with pancreatic cancer. METHODS:Real-time fluorescence quantitative RT-PCR was applied to investigate the quantitative expression of hTERT and MUC4 mRNAs in peripheral blood of patients with pancreatic cancer and healthy controls. RESULTS:The expression levels of hTERT and MUC4 mRNAs in peripheral blood of patients with pancreatic cancer were significantly higher than those in normal controls (7.95 ± 5.46 vs 0.92 ± 1.07, P < 0.01; 38.25 ± 25.07 vs 4.37 ± 5.96, P < 0.05). The expression of both hTERT and MUC4 mRNAs was associated with lymph node metastasis, surrounding organ infiltration, distant metastasis, and TNM stage (hP = 0.036, 0.027, 0.019; M P = 0.041, 0.022, 0.017). CONCLUSION:Abnormal expression of hTERT and MUC4 mRNAs closely correlates with invasion and metastasis of pancreatic cancer, and hTERT and MUC4 mRNAs in peripheral blood cells might be useful markers for pancreatic caner.

目的：探讨中国胆管乳头状瘤的流行病学特征及临床特点。方法：联合检索维普数据库和中国知网等多家中文数据库中国1979～2011年有关胆管乳头状瘤的文献，总结分析胆管乳头状瘤的临床流行病学特征。结果：共报道胆管乳头状瘤39例，男女比例为1．06：1，平均年龄60岁，高发于50～70岁，74．4％分布于华东地区，17.2％分布于西南地区，以腹痛、黄疸为主要症状。肿瘤根治性切除是最主要的治疗手段。结论：胆管乳头状瘤主要分布在华东、西南地区，发病率较低，术前诊断较困难，易被误诊，手术切除是治疗此病有效方法，手术以肿瘤根治性切除为主。

Objective: To investigate the epidemiological features,diagnosis and treatment of splenic inflammatory myofibroblastic tumor in China during the past 25 years.Methods: The clinical data of 64 patients diagnosed splenic inflammatory myofibroblastic tumor(Splenic inflammatory pseudotumor) published from 1985 to 2010 from various databases,including China National Knowledge Infrastructure(CNKI),VIP,and the epidemiological features,diagnosis and treatment of SIMT patients were retrospectively analyzed.Results: There were 64 patients,including 39 males and 25 females(1.56:1).And their age spread from 24 to 74 with an average of 49.2.Most of patients had not special symptoms,and were diagnosed through routine examinations such as type-B ultrasonic or CT scan.Epigastric pain,debilitation,low-grade fever and some other untypical symptoms might appear in some patients.A solitary splenic lesion might be seen during medical imageologcal examination.Histological types: hypocellular fibrous pattern(n=26),myxoid/vascular pattern(n=12),compact spindle cell pattern(n=5).Immunophenotypic findings show that the spindle cells were positive for Vimentin,SMA in most cases.For most patients,splenectomy were the mainly treatment,and there were no signs of relapse and metastasis after the operation.Conclusion: Splenic inflammatory myofibroblastic tumor is extremely rare in china,and there are not special clinical symptoms or signs.It can be identified by characteristics of medical imaging,histologic and immunophenotypic findings with other splenic lesions.But it is difficult to define its nature preoperatively.Splenectomy is the mainly treatment with a satisfactory prognosis.

脾脏炎性肌纤维母细胞瘤(splenic inflammatory myofibroblastic tumor,SIMT)是一种少见的脾脏肿瘤,多发于中老年人,预后良好.我院2008年1月至2011年10月共收治SIMT患者5例,现报道如下.临床资料1.一般资料:本组患者男4例,女1例.年龄30～53岁,平均40.8岁.临床表现及病史:3例查体时B超发现脾占位性病变,另外2例表现为左上腹疼痛.病程最短1d,最长3个月。