To assure the pathogenic bacteria of white-spot disease from skin surface in Gymnocypris prze-waiskii and treat the disease,three bacterial strains (Citrobacter freundii ,Aeromonas veronii ,Morganella fulton)were isolated from naturally infected Gymnocypris przewaiskii and identified by morphology,cul-tural characteristics,physical and chemical characters,and 16 S rRNA sequence analysis.The three strains were Gram negative.Colony morphologies were consistent.Drug sensitivity test revealed that the three strains were highly sensitive to cefepime,amikacin and gentamicin,which will benefit the treatment of dis-ease in Gymnocypris przewaiskii .

青海湖是我国第一大内陆湖泊和最大的咸水湖,湖区内生存的主要鱼种为裸鲤,属硬骨鱼纲、鲤形目、鲤科、裂腹鱼亚科,此鱼种仪分布于中同青海湖及其邻近水系,是高原低温盐碱性水域经济鱼类.青海湖甘子河位于海晏县两北部,海拔3000～3500米,以盛产裸鲤而闻名,鱼类资源十分丰富.本项目组于2013年开始共剖检了裸鲤128尾,收集体内寄生虫,鉴定种类,统计感染率、感染强度、平均丰度,分析季节动态.为丰富青海省渔业资源的保护,对寄生虫给裸鲤养殖业带来的危害提供理论依据.

BL-420F生物机能实验系统是一台程序可控的,带4通道生物信号采集与放大功能,并集成高精度、高可靠性以及宽适应范围的程控刺激器于一体的处理系统。在机能学实验中以其功能强大、灵活、实验结果客观可信,为机能学经典实验的简化、深化及综合性实验的开展提供了有力的支持。

为了掌握青海省大通种牛场和海晏县牦牛病毒性腹泻/黏膜病的感染和流行情况,我们于2010年3月至5月对大通种牛场和海晏地区的252份牦牛血清样品,其中海晏县141份,大通种牛场111份,应用定量酶联免疫吸附试验(ELISA)进行牛病毒性腹泻/黏膜病感染的血清抗体检测。结果:大通种牛场牦牛群中阳性26份,阳性率为23.42%(26/111),可疑9份,可疑率8.11%(9/111);海晏县阳性28份,阳性率为19.86%(28/141),可疑15份,可疑率为10.64%(15/141),其中金滩村、托勒村和德州村牦牛群中阳性分别为12份、11份和5份,阳性率分别为22.36%(12/41)、22.00%(11/50)和10.00%(5/50),可疑分别为9份、5份和1份,可疑率分别为21.95%(9/41)、10.00%(5/50)和2.00%(2/50)。

The seral antibodies of Mycoplasma gallisepticum were detected from 286 samples collected from the area of Huangzhong,Huangyuan,Datong and Xining by ELISA.The results showed the positive samples were 146,and the average positive rate was 51.08%(146/286).

By using indirecthemagglutination assay(IHA),100 serum samples from 7 areas yak farms of Huzhu county were tested for antibody against Toxoplasma gondi.i.The results were as follow:Average infection rate of 12.15 tested sera antibody titers were≥1:64 which was regarded as positive.Simultaneously,infection rate which yak were different sexes had no significant difference showed that infection rate had no reference to sexes.

<正>1.2.2热应激对母猪繁殖力的影响1.2.2.1对发情的影响母猪受热应激后常出现乏情和行为乏情(即无行为发情而排卵),其发情期稍延长。1.2.2.2对配种的影响母猪配种前后三周受热应

<正>鸡败血支原体感染是由鸡败血支原体引起的,其特征为呼吸困难、咳嗽、流鼻液,病程发展缓慢,成年鸡多为隐性感染,可在鸡群中长期存在和蔓延,感染此病后,幼鸡生长发育不良,成年鸡产蛋量下降,并且由于

Pathogenic bacteria of liver,spleen,lung and kidney frouw case fatality sheep were isolated and identified.The same time Pathological tissue was examined.The colibacillosis in Han-sheep with small tail was diagnosed according epidemiological information,clinical symptoms and results of laboratory examination.

2004年10月17日,某地耕牛突然死亡,据临床症状,怀疑为炭疽(未进行尸体剖检).经流行病学、死后变化和实验室检查,诊断为炭疽.

Pathogenic bacteria were isolated from eleven yaks suspected dying of Anthrax and then identified. Integrating with epidemiological survey, clinical symptom and examination in laboratory, it was diagnosed that the yaks were mixed infection with Anthrax bacillus and Pasteuria.

大肠杆菌病是由致病性大肠杆菌引起的一类多种动物共患的传染病,尤其是幼龄动物多发.因致病性大肠杆菌的血清型不同,表现的病理过程和临床症状也不一致.一般以肠炎较多见,有时还可引起败血症和毒血症,引起动物死亡,或者严重影响幼畜生长发育,给畜牧业发展造成重大损失[1].

随着养殖业的飞速发展,饲料行业原料短缺的状况越来越突出.粗纤维作为自然界中含量最丰富、来源最广泛,又可再生的饲料资源,由于利用率低而至今未得到充分利用.因此,如何成功地开发和利用纤维素资源是目前饲料资源开发的关键.国内外研究推广的利用纤维素的方法很多,如微生态制剂发酵、氮化、酶降解法等.但通过利用纤维素酶或复合酶制剂达到有效利用,则成为国内外动物营养学家极为关注的课题.

1997年10月某牧场两头经产奶牛相继发生流产,因无机械性刺激,饲料及饮水正常,也未曾用任何药物,所以排除以上诸因素引起流产的可能,进行了细菌学和血清学诊断,确诊为布氏杆菌引起的流产,可能是精液中布氏杆菌所致.现将诊断情况报告如下.

EM活菌剂由光合细菌、乳酸菌和放线菌等10属80多种微生物培养而成的有效微生物群.它们相互组合在一起,形成一个复杂的微生物生态系统.这个系统犹如一个微生物加工厂,各种微生物在其生长过程中产生出的有用物质及其分泌物质,或为各自或相互生长的基质或原料,从而形成相互间的共生增殖关系,显示出显著的经济生态效益和社会效益.EM在养殖业中的作用主要表现在: