Purpose: To investigate the effects of circ_0001953/miR-186 on human retinal vascular endothelial cell (HRVEC) injury evoked by high glucose. Methods: A cell model (HG group) was established using high glucose-treated HRVECs, while untreated HRVECs were used as the control group (Con group). The levels of endothelin-1 (ET-1), ICAM-1 and IL-6 were evaluated by ELISA and the content of malondialdehyde (MDA) and superoxide dismutase (SOD) in HRVECs were determined. Apoptosis rate was tested adopting flow cytometry. The interrelationship between circ_0001953 and miR-186 was assessed using dual luciferase reporter assay. Measurement of Bax and Bcl-2 was implemented via western blot. Results: In HG group, circ_0001953 increased while miR-186 was downregulated, ET-1, IL-6, ICAM-1, and apoptosis rate increased and accompanied with up-regulated Bax content and declined Bcl-2 protein level. Furthermore, the content of MDA increased and SOD decreased. MiR-186 was a target of circ_0001953. Conclusion: Inhibition of circ_0001953 can repress inflammation, oxidative stress and apoptosis in HRVECs by up-regulating the expression of miR-186.

This retrospective case-control study examined the association between the rs2071559 (-604T/C) single nucleotide polymorphism (SNP) in the vascular endothelial growth factor receptor (VEGFR)-2 gene and the risk of diabetic retinopathy (DR) in Northern Han Chinese. This study included patients diagnosed with diabetes mellitus (DM) in Shijiazhuang between 07/2014 and 07/2016. The healthy controls were unrelated individuals who received routine physical examinations. The diabetic patients were grouped as DM (diabetes but no fundus examination abnormalities), proliferative DR (PDR), and non-proliferative DR (NPDR). Finally, 438 patients were included: 114 controls and 123, 105, and 96 patients in the DM, NPDR, and PDR groups, respectively. In the multivariable analyses and all genetic models, the VEGFR-2 rs2071559 SNP was not associated with DR (among all diabetic patients) or with PDR (among the patients with DR) after adjustment for age, sex, duration of DM, blood glucose, systolic blood pressure, diastolic blood pressure, and body mass index (all P > 0.05). In conclusion, the VEGFR-2- 604T/C rs2071559 SNP is not associated with DR or PDR in the Han Chinese population of Shijiazhuang (China).

Purpose: To investigate the clinical effect of sodium hyaluronate eye drops combined with tobramycin, dexamethasone, and pranoprofen eye drops in the treatment of dry eye after phacoemulsification. Methods: Medical records of 188 patients with dry eye after phacoemulsification, treated in our hospital from August 2020 to August 2021, were included and divided into groups based on the type of dry eye treatment. Patients in the control group (n = 90) were treated with tobramycin, dexamethasone, and pranoprofen eye drops. Patients in the test group (n = 98) were treated with sodium hyaluronate, tobramycin, dexamethasone, and pranoprofen eye drops. The tear secretion test (TST), film rupture time (BUT), corneal fluorescence staining (FS) score, levels of interleukin (IL)-6, and tumor necrosis factor (TNF)-α before the treatment and at 1 month follow-up, the overall total effective rate and the number of completely cured cases were compared between the two groups. Multiple linear regression was used to calculate coefficients for predicting clinical variables. Ordinal logistic regression was used to compute coefficients and odds ratios for predicting effective scores. Results: The total effective rate in the observation group (99%) was significantly higher than that in the control group (80.00%). After the treatment, the TST and BUT indexes of the observation group were higher compared to the control group, and the FS score of the observation group was lower than that of the control group. TNF-α and IL-6 levels of the observation group were lower compared to the control group. Logistic regression analysis demonstrated that the differences in the effective rate and clinical variables between the two groups remained significant after adjusting for the effect of age. Conclusion: A combination of sodium hyaluronate, tobramycin, dexamethasone, and pranoprofen eye drops for the treatment of dry eye after phacoemulsification can improve the curative effect, reduce corneal fluorescein staining and inflammatory factor index levels compared to the treatment regimen lacking sodium hyaluronate eye drops.

In order to explore the function of inhibiting the immune effect, the relationship between programmed death receptor 1 (PD-1) carrelizumab in the treatment of hepatocellular carcinoma-induced scleritis and T cell activation is investigated. A total of 120 patients with primary liver cancer treated in the department of oncology of our hospital from July 2020 to January 2022 are selected and treated with carrelizumab. According to the occurrence of PD-1 carrelizumab treatment, the patients are divided into the scleritis group and nonscleritis group. The levels of T cells, PD-1, PD-L1 proteins, and serum inflammatory factors at different time points are compared. The experimental results show that the occurrence of scleritis after liver cancer treatment with PD-1 carrelizumab is closely associated with Treg cells, the percentage of Th17 cells, the expression of PD-1, PD-L1 proteins, and inflammatory factors. It is clearly evident that PD-1 carrelizumab can increase the risk of scleritis by affecting T cell activation.

我今年50岁,前阵子发现眼睑处长了几个淡黄色的肿块,医生说是眼睑黄色瘤.请问,什么是眼睑黄色瘤?该如何治疗? 吉林长春 何玉梅 眼睑黄色瘤是中老年人常见的皮肤病,亦称睑黄疣,表现为两侧眼睑对称性分布的橘黄色米粒大小或黄豆大小的丘疹,边缘清晰,质地柔软,并有逐渐增长和互相融合的趋势.无自觉症状,不能自行消退,也不引起功能障碍.多见于中年女性,尤其是更年期前后.

BackgroundYoung children’s vision screening, as part of a preventative health care service, produces great value for developing regions. Besides yielding a high return on investment from forestalling surgeries using a low-cost intervention at a young age, it improves school performance and thus boosts future labor force quality. Leveraging low-skilled health care workers with smartphones and automated diagnosis to offer such programs can be a scalable model in resource-limited areas. ObjectiveThis study aimed to develop and evaluate an effective, efficient, and comprehensive vision screening solution for school children in resource-limited areas. First, such an exam would need to cover the major risk factors of amblyopia and myopia, 2 major sources of vision impairment effectively preventable at a young age. Second, the solution must be integrated with digital patient record-keeping for long-term monitoring and popular statistical analysis. Last, it should utilize low-skilled technicians and only low-cost tools that are available in a typical school in developing regions, without compromising quality or efficiency. MethodsA workflow for the screening program was designed and a smartphone app was developed to implement it. In the standardized screening procedure, a young child went through the smartphone-based photoscreening in a dark room. The child held a smartphone in front of their forehead, displaying pre-entered personal information as a quick response code that duplexed as a reference of scale. In one 10-second procedure, the child’s personal information and interpupillary distance, relative visual axis alignment, and refractive error ranges were measured and analyzed automatically using image processing and artificial intelligence algorithms. The child’s risk for strabismus, myopia, and anisometropia was then derived and consultation given. ResultsA preliminary evaluation of the solution was conducted alongside yearly physical exams in Luoyang, Henan, People’s Republic of China. It covered 20 students with suspected strabismus and 80 randomly selected students, aged evenly between 8 and 10. Each examinee took about 1 minute, and a streamlined workflow allowed 3 exams to run in parallel. The 1-shot and 2-shot measurement success rates were 87% and 100%, respectively. The sensitivity and specificity of strabismus detection were 0.80 and 0.98, respectively. The sensitivity and specificity of myopia detection were 0.83 and 1.00, respectively. The sensitivity and specificity of anisometropia detection were 0.80 and 1.00, respectively. ConclusionsThe proposed vision screening program is effective, efficient, and scalable. Compared with previously published studies on utilizing a smartphone for an automated Hirschberg test and photorefraction screening, this comprehensive solution is optimized for practicality and robustness, and is thus better ready-to-deploy. Our evaluation validated the achievement of the program’s design specifications.

Objective: To investigate how VEGF-634G/C and VEGF-460C/T SNPs are related to diabetic retinopathy (DR) in Han Chinese subjects from the Shijiazhuang region of China.Methods: Totally 376 DM cases were divided into non-proliferative diabetic retinopathy (NPDR) group (n = 124), proliferative diabetic retinopathy (PDR) group (n = 108), and diabetes without retinopathy (DWR) group (n = 144). PCR/LDR was utilised to detect and assess the genotypes and allele distribution frequencies at the VEGF-634G/C and VEGF-460C/T loci in each group.Results: The differences between NPDR, PDR and DWR groups were not significant in genotypes and allele distribution frequencies at VEGF-634G/C locus (P > 0.05). But there were significant differences between NPDR and DWR groups in genotypes (P = 0.013) and allele distribution frequencies (P = 0.002) at VEGF-460C/T locus, at which CT + CC genotypes were associated with a reduced risk of developing NPDR. There were no significant differences in genotypes (P = 0.759) or allele distribution frequencies (P = 0.433) at VEGF-460C/T locus between PDR and DWR groups.Conclusions: Among Chinese Han individuals with type-2 DM, polymorphism-634G/C of the VEGF gene was not correlated with NPDR or PDR; however, polymorphism-460C/T of the VEGF gene was correlated with NPDR, and C allele was associated with lower NPDR risk than T allele. (C) 2014 Elsevier Inc. All rights reserved.

目的 研究屈光不正、眼轴长度与糖尿病视网膜病变(DR)的相关性.方法 随机选取2011年5月至2011年8月常规眼科会诊的65例2型糖尿病患者,应用自动验光仪测得屈光度,分为正视眼(-0.5D≤屈光度≤1D)、低度近视(-5D＜屈光度＜-0.5D)、高度近视(屈光度≤-5D)、远视(屈光度＞1D),A型超声测得眼轴长度,以眼底照相进行DR分级:轻度DR,中度DR,威胁视力的DR.结果 经校正年龄、性别、白内障、糖化血红蛋白(HbA1c)等多变量后,近视眼更不易患轻度DR(P=0.003,per 1-D decrease)、中度DR(P=0.002)、威胁视力的DR(P=0.001).眼轴越长越不易患any DR(P=0.039,per 1-mm increase),moderate DR(P=0.201),and vision-threatening DR(P=0.042).结论　近视及眼轴长不易患DR,尤其是威胁视力的DR,是DR保护性因素.

目的评价和探讨渐进多焦镜与角膜塑形镜控制青少年近视进展的作用。方法1.00D～-6.00D的青少年近视患者120例,随机分为2组,Ⅰ组配戴近用附加度数+1.50D渐进多焦镜,Ⅱ组配戴角膜塑形镜。观察3年治疗期满时,停戴角膜塑形镜1个月后与同期配戴渐进多焦镜3年的Ⅰ组比较屈光度及眼轴的变化。结果3年后,Ⅰ组平均近视屈光度增加(0.84±0.49)D,Ⅱ组平均近视屈光度增加(0.49±0.68)D,2组比较差异有统计学意义(P<0.01)。而眼轴长度Ⅰ组平均增长(0.46±0.11)mm,Ⅱ组平均增长(0.29±0.15)mm,2组比较差异有统计学意义(P<0.01)。结论角膜塑形镜较渐进多焦镜更能有效减缓青少年近视的发展。

近视是指眼处在静态屈光时,无限远点成像在视网膜之前.近视在成年之前是一个不断发展,逐渐加深的过程.近年来,我国青少年近视眼患病率不断攀升,近视患者升学,参军和工作都会受到限制,近视眼的防治成为一个全球性的医学和社会问题.

目的 研究拉坦前列素和噻吗心安连续应用12周对原发性开角型青光眼患者降眼压作用和对眼血流的影响.方法 选择原发性开角型青光眼患者218例,随机分成2组:拉坦前列素组和噻吗心安组.分别滴用0.005%拉坦前列素每日1次和0.5%噻吗心安每日2次,疗程均为12周.观察眼压及眼血流动力学参数变化.结果 2组患者治疗后眼压均有明显下降,且眼压下降平稳恒定;但拉坦前列素组降眼压作用更强大(P<0.05).拉坦前列素组视网膜中央动脉(CRA)和睫状后动脉(PCA)的各项血流动力学指标与治疗前相比有显著差异(P<0.05),血流的收缩期峰值流速(PSA)和舒张末期流速(EDV)较治疗前均增加,阻力指数(RI)较治疗前降低,而噻吗心安组CRA和PCA的各项血流动力学指标与治疗前相比差异无统计学意义(P>0.05).结论 拉坦前列素对原发性开角型青光眼患者降眼压疗效及改善眼血流作用优于噻吗心安,且无明显毒、副作用,是理想的抗青光眼药物.

近年来的研究提示,渐进多焦镜能延缓或控制青少年近视的发展[1],是青少年近视防治的一种安全、有效的方法.为进一步探讨渐进多焦镜对控制青少年近视发展的效果,我院对青少年近视眼配戴渐进多焦镜,取得了良好的疗效.

目的:研究适利达(拉坦前列素)和噻吗心安连续应用12周对原发性开角型青光眼患者眼压和眼血流的影响,并观察其安全性。方法:选择原发性开角型青光眼患者91例,随机分成适利达组和噻吗心安组。分别滴用0.005%适利达每日1次和0.5%噻吗心安每日2次,疗程均为12周。治疗前、治疗后2周、6周及12周随访,测量眼压并观察眼局部及全身不良反应;并于治疗前及治疗后12周应用彩色超声多普勒成像系统测量视网膜中央动脉(CRA)和睫状后短动脉(PCA)的血流动力学参数变化。结果:两组患者治疗后眼压均有明显下降,且眼压下降平稳恒定;但适利达组降眼压作用更强大(P<0.05)。适利达组CRA和PCA的各项血流动力学指标与治疗前相比差异有显著性(P<0.05),血流的收缩期峰值血流速度和舒张末期血流速度较治疗前均增加,血管阻力指数(RI)较治疗前降低。而噻吗心安组CRA和PCA的各项血流动力学指标与治疗前相比差异无显著性(P>0.05)。结论:适利达对原发性开角型青光眼患者降眼压疗效及改善眼血流作用优于噻吗心安,且无明显毒副作用,是理想的抗青光眼药物。

目的比较氪激光治疗与氪激光联合球旁注射曲安奈德治疗视网膜静脉阻塞的疗效。方法45例视网膜静脉阻塞患者45只眼随机分为治疗组与对照组。对照组氪黄与氪绿进行眼部播散性光凝,治疗组在研究开始及第4周应用特制球后注射针头将曲安耐德20mg注入到后部巩膜旁间隙,激光治疗同对照组。随访3个月以上,对比观察治疗前后患者的视力、出血吸收情况及黄斑水肿消退情况。结果第1、2、3月治疗组视力恢复情况和眼部综合检查分值与对照组比较均有统计学意义。结论氪激光联合球旁注射曲安奈德较单纯激光治疗视网膜分支静脉阻塞起效快,后极部水肿及出血明显减轻,能缩短患者病程,使患者视功能尽早恢复及提高,减小糖皮质激素玻璃体腔内注射引起白内障和眼压升高药物的副作用。

Objective To observe the changes of ocular blood flow by continuous latanoprost and brimonidine eyedrops for 12 weeks.Methods 69 patients with open-angle glaucoma were divided randomly into two groups,one group treated with 0.005% latanoprost drops once a day,another group treat with 0.2%brimonidine twice a day.Peak-systolic velocity(PSV),end-diastolic velocity(EDV)and resistivity index(RI)in the short posterior ciliary artery(PCA),central retinal artery(CRA)were recorded respectively by colour doppler imaging(CDI).Results The peak-systolic velocity and the end-diastolic velocity of central retinal arteries and the short posterior ciliary artery were significantly higher after application of latanoprost,while resistivity index was decreased(P0.05).There was no significant difference in brimonidine group before and after treatment(P0.05).After 12 weeks of treatment,PSV and EDV of PCA was higher and RI was lower in latanoprost group compared with brimonidine group,(P0.05).For CRA,PSV of latanoprost group was higher than that of brimonidine group(P0.05),while EDV and RI were no significant difference in two groups(P0.05).Conclusion Latanoprost should be regarded as a useful drug for treatment of glaucoma.It could improve the ocular blood supply,and has a stronger effect than that of brimonidine.

患者,男性,52岁,突然出现右眼红、羞明、流泪、长眼屎,对症给予消炎眼水点眼,症状无缓解2月余.初起时有疼痛用药后消失.曾被多家医院诊断为急性结膜炎,给予抗生素及抗病毒药和激素类眼药水及保护角膜药物频繁点眼,眼部症状未见改善和加重,但眼内分泌物渐增多影响视物,需经常擦拭,在门诊做细菌培养为铜绿假单胞菌,收入院.