Chronic non-bacterial prostatitis (CNP), a prevalent and debilitating urological disorder affecting 8.4% of men aged 15-60 years, presents significant clinical challenges due to the paucity of targeted therapies and poor patient adherence. To address this unmet medical need, we developed an innovative multifunctional nanoplatform (QM (Zn) NPs) by integrating Ti3C2 MXene with a quercetin-zinc coordination complex (Que-Zn) for precision CNP therapy. This system leverages chondroitin sulfate (Chs)-mediated CD44 targeting to achieve selective accumulation in inflamed prostate tissue, thereby enhancing Zn2+ bioavailability while enabling co-delivery of MXene and Que-Zn therapeutic payloads. Upon localization, QM (Zn) NPs orchestrate a coordinated therapeutic cascade: MXene scavenges reactive oxygen species (ROS) via electron-deficient sites, while Que-Zn drives M1-to-M2 macrophage repolarization and facilitates Zn2+ cellular uptake. The accumulated intracellular Zn2+ critically upregulates metallothionein 1 (Mt1), activating the IKK/NF-κB/IκB axis to resolve inflammation and oxidative damage. Transcriptomic analysis unequivocally identified Mt1 as the pivotal mediator of Zn2+-driven microenvironment reprogramming. Notably, QM (Zn) NPs not only significantly alleviated pelvic pain by mitigating neuronal oxidative stress but also exhibited excellent biocompatibility. This work pioneers a targeted nano-theranostic strategy that synergistically restores zinc homeostasis, quenches ROS, and reprograms immune responses, thereby establishing a transformative paradigm for CNP management.

Bacterial prostatitis represents a specific form of prostatitis, primarily resulting from bacterial infection and significantly impairing the life quality of patients. In this paper, we respond to the inability of conventional drugs to simultaneously address both bacterial infection and oxidative stress in the treatment of prostatitis by designing a multifunctional nanoparticle, called QM (Cu) NPs, with dual functionality. QM (Cu) NPs have the capacity to generate reactive oxygen radicals to eradicate bacteria under the influence of laser irradiation. Additionally, they are capable of rapidly scavenging the surplus free radicals, thereby restoring the intracellular redox homeostasis in the absence of laser illumination. A comprehensive characterization of QM (Cu) NPs was conducted, followed by an in-depth analysis of their effects on cells. The therapeutic efficacy of QM (Cu) NPs in multimodal treating bacterial prostatitis was then demonstrated. Furthermore, the outcomes of transcriptomic and molecular biology experiments indicated that QM (Cu) NPs markedly regulate the NF-κB p65 and Nrf2-Keap1 signaling pathways, thereby influencing inflammatory and oxidative stress processes. In conclusion, QM (Cu) NPs simultaneously addressed the dual challenges of antibacterial and antioxidant properties, thereby underscoring their potential clinical applications in the treatment of bacterial prostatitis.

Bacterial prostatitis is a common disease of the male genitourinary system, which seriously affects the normal life and health of male patients. Antibiotics are commonly used in the clinical treatment of bacterial prostatitis, but the efficacy of fluoroquinolones is gradually declining due to the increasing drug resistance of bacteria. Hence, it is necessary to find new antibacterial drugs to treat bacterial prostatitis. Luteolin is a natural flavonoid compound with many pharmacological activities such as antibacterial and anti-inflammatory activities, but its poor water solubility and low structural stability seriously limit its clinical application. In this study, we designed a targeting drug delivery system via a luteolin-copper complex grafted with hyaluronic acid. The results of the characterization proved the successful synthesis of the system. The results of the in vitro performance test show that the system has a good antibacterial effect and excellent blood compatibility and can be effectively released under different pH conditions. The prepared nanodrug delivery system not only provides a new idea for the treatment of bacterial prostatitis but also lays a theoretical and practical foundation for the wide application of luteolin in clinical practice.

Postoperative adhesion is a common clinical disease caused by surgical trauma, accompanying serious subsequent complications. Current non-surgical drug therapy and biomaterial barrier administration have limited therapeutic effects due to their inherent deficiencies. Therefore, developing a simple, effective, and feasible method to effectively prevent postoperative adhesions after surgical procedures remains a challenge. An injectable chondroitin sulfate complex hydrogel was prepared based on aldehyde-modified chondroitin sulfate (ChS-CHO) and hydrazine-modified chondroitin sulfate (ChS-ADH). The hydrogel showed enhanced strength and good self-healing ability. By using the Schiff base reaction principle that aldehyde group reacts with hydrazide to form hydrazone bond, C-A hydrogel physical barrier is formed at the wound site to reduce the occurrence of postoperative adhesion. There is no use of chemical crosslinkers in the whole reaction system to prepare C-A hydrogel, which has excellent biocompatibility and is safe and non-toxic. The results showed that C-A hydrogel showed excellent mechanical properties, good self-healing, and biocompatibility. The cecal-abdominal wall adhesion model and hepatic adhesion model of rats were constructed respectively to evaluate its preventive effect on postoperative adhesion. The results showed that C-A hydrogel had a more significant preventive effect on postoperative adhesion, and appears to be a promising candidate for postoperative adhesion.

目的 评估硕通镜联合软式输尿管镜治疗长径＜20 mm肾结石在日间手术模式中的临床效果.方法 选取2019年1月至2020年12月本院就诊的110例单侧结石长径＜20 mm的肾结石患者作为研究对象,随机分为日间手术模式组和常规手术模式组,每组各55例.观察两组的术中置鞘成功率、手术时间、住院时间、住院费用、术后并发症发生率、术后1个月结石清除率以及术后3个月患者满意度.结果 两组共5例患者因置鞘失败改行经皮肾镜碎石术.其余患者均顺利完成手术.日间手术模式组与常规手术模式组患者的术中置鞘成功率、手术时间、术后并发症发生率、术后1个月结石清除率比较,差异均无统计学意义(均P＞0.05).与常规手术模式组比较,日间手术模式组患者住院时间更短,总住院费更低,患者对治疗过程满意度更高,差异均有统计学意义(均P＜0.05).结论 日间手术模式下行硕通镜联合软式输尿管镜治疗结石长径＜20 mm肾结石可以缩短治疗周期、减轻患者经济压力,不增加手术风险,且提高了患者的满意度,值得在有条件的医院推广.

Ulcerative colitis (UC) is a chronic nonspecific inflammatory bowel disease often characterized by rapid progression and frequent comorbidities that make its treatment challenging. In colonic ulcers of UC patients, myeloperoxidase (MPO) is highly expressed, which results in an abundance of macrophages and reactive oxygen species. This study developed an active MPO-targeting hyaluronic acid/serotonin ceria nanoenzyme (HA-5-HT@CeO2) using the electrostatic interaction between CeO2 nanoparticles, 5-hydroxyserotonin-cerium oxide and hyaluronic acid. Based on the dual targeting effects of MPO and the macrophage CD44+ receptor in locating the inflammatory site in conjunction with the inflammatory area of the colon through electrostatic action, CeO2 nanoparticles along with multiple similar enzymes were used to eliminate O2, H2O2 and ˙OH and other reactive oxygen species, achieving targeted repair of the intestinal epithelial barrier through the elimination of inflammatory factors. In studies involving pharmacodynamics in vitro and DSS-induced animal models of acute colitis in vivo, HA-5-HT@CeO2 has been shown to reduce inflammation further and treat ulcerative colitis compared to traditional drugs. Additionally, active targeting of MPO inflammation can lead to accurate drug delivery to the site and can minimize the side effects associated with the drug. HA-5-HT@CeO2 is a promising novel drug for the treatment of ulcerative colitis. In addition to illustrating the benefits of this novel nanodrug delivery in treating ulcerative colitis compared to traditional medications, this study provides theoretical and experimental support for its application to any targeted therapy for ulcerative colitis.

目的 分析Snail、Stathmin的表达与前列腺癌患者的组织学分级、临床分期的相关性及对患者术后生存率的影响.方法 选择本院2018年12月至2021年5月间收治的186例前列腺癌患者作为观察组,选择同期100例前列腺增生患者作为对照组;对两组样品行Snail、Stathmin免疫组化测定;采用Spearman相关性检验分析Snail、Stathmin的表达与前列腺癌患者组织学分级、临床分期的相关性;绘制卡米尔生存曲线分析Snail、Stathmin表达情况与患者术后生存率的关系.结果 观察组的Snail和Stathmin阳性表达情况明显高于对照组,差异均有统计学意义(均P＜0.05);不同组织学分期患者的Snail、Stathmin表达呈明显差异,其中G3期Snail、Stathmin阳性表达最高,G1期最低,差异均有统计学意义(均P＜0.05);不同临床分期患者的Snail、Stathmin表达存在明显差异,其中D期患者的Snail、Stathmin阳性表达最高,A期阳性表达最低,差异均有统计学意义(均P＜0.05);前列腺癌患者的临床分期、组织学分级与Snail、Stathmin表达呈明显正相关(均P＜0.05);Snail阴性或弱阳性和Stathmin阴性或弱阳性患者的术后无进展生存期明显高于Snail阳性或强阳性和Stathmin阳性或强阳性患者,差异均有统计学意义(均P＜0.05).结论 前列腺癌的临床分期、组织学分级与Snail、Stathmin表达呈明显正相关,且Snail、Stathmin高表达可明显降低患者的术后生存率.

Bacterial prostatitis is a bacterial infection of the prostate gland presenting with lower quadrant abdominal pain, urination disorders and poor fertility. In recent years, reports have emerged on the significantly reduced efficacy of fluoroquinolone drugs attributed to multiple drug-resistant bacteria, emphasizing the need for new drugs. In this study, we designed a targeting drug delivery system via curcumin copper complex grafted with hyaluronic acid. Subsequently, the prepared system was characterized using FT-IR, XRD, SEM, XPS and 1H NMR methods. In addition to the substantial improvement in the solubility of the carrier, its antibacterial performance and targeting ability were improved. Interestingly, the grafting of hyaluronic acid endowed the carrier with excellent CD44 receptor targeting function and good water solubility, and the complexation of copper ions greatly enhanced its antibacterial capability, especially the inhibitory effect on E. coli. The anti-prostatitis effect of the drug was evaluated comprehensively by establishing a bacterial prostatitis model infected by E. coli. Assessment of the anti-prostatitis effects in vivo indicated that the Cur-Cu@HA delivery system could effectively promote recovery from bacterial prostatitis by downregulating inflammation. In conclusion, our Cur-Cu@HA delivery system has great potential for treating bacterial prostatitis.

目的 探讨肾肿瘤剜除术对局限性肾癌病人肾功能、肾实质及畸胎瘤衍生生长因子-1(Cripto-1)的影响.方法 2016 年2 月～2021 年6 月我院确诊并进行治疗的局限性肾癌病人100 例,根据治疗方法不同将病人分为研究组(60 例)和对照组(40 例).研究组采用腹腔镜肾肿瘤剜除术,对照组采用开放肾部分切除术.记录两组病人手术时间、肾动脉阻断及住院时间、术中出血量,比较两组病人手术前后肾功能、肾实质与血清Cripto-1 水平.结果 研究组病人术中出血量(89.65±20.22)ml,住院时间(8.25±2.25)天,对照组分别为(126.69±25.63)ml和(11.40±3.52)天,两组比较,差异有统计学意义(P＜0.05).术后3 个月研究组肾小球滤过率(GFR)、尿素氮(BUN)、肌酐(Scr)水平分别为(85.24±2.25)ml/min、(10.47±2.14)mmol/L、(80.65±10.28)μmol/L,对照组分别为(118.65±3.20)ml/min、(13.41±2.25)mmol/L、(97.45±11.25)μmol/L,两组比较,差异有统计学意义(P＜0.05).术后研究组肾实质体积丢失值积(9.86±2.20)cm3,低于对照组的(15.96±3.25)cm3,差异有统计学意义(P＜0.05).术后 3 个月,研究组血清Cripto-1 水平(1.01±0.55)ng/ml,低于对照组的(2.55±0.85)ng/ml,差异有统计学意义(P＜0.05).结论 肾肿瘤剜除术能有效减少局限性肾癌病人术中出血量,缩短住院时间,降低病人血清Cripto-1 水平,同时保留更多的正常肾实质,改善肾功能.

It is found that HDAC3 may be a potential therapeutic target for intestinal related diseases. At present, the role and mechanism of HDAC3 in the pathogenesis of severe acute pancreatitis (SAP) have not been reported, which needs to be further explored. The SAP mouse model was established and the expression of HDAC3 was detected by immunohistochemistry. H&E staining showed the intestinal pathological state of SAP mice. The expression of HDAC3 was measured by real-time quantitative PCR (RT qPCR) and Western blot. Apoptosis kit was used to determine cell apoptosis rate. The level of inflammatory factors was detected by ELISA kits. The expressions of HDAC3, cGAS and Sting were significantly increased in SAP patients and SAP mice. Silencing HDAC3 promoted the proliferation and adhesion of intestinal glial cells and inhibited the inflammation and apoptosis of intestinal epithelial cells. In addition, silencing HDAC3 inhibited oxidative stress in intestinal epithelial cells. Furthermore, silencing HDAC3 inhibited the activation of cGAS-Sting pathway in intestinal glial cells. More importantly, silencing HDAC3 alleviates intestinal barrier function in SAP mice. HDAC3 inhibition improves acute pancreatitis in mice by regulating cGAS-Sting pathway of intestinal glial cells.

目的 探讨细丝蛋白C(filamin C,FLNC)对前列腺癌细胞迁移和侵袭的影响.方法 通过免疫组织化学分析了30例前列腺癌患者的癌组织及配对癌旁组织中FLNC的表达.根据FLNC蛋白的表达水平将30例前列腺癌患者分为FLNC低表达组(0～3分,n=9)和FLNC高表达组(4～12分,n=21),采用Kaplan-Meier法进行生存分析.对人前列腺癌细胞系(PC-3)转染抑制FLNC表达的小干扰RNA(siRNA-FLNC组)和阴性对照(siRNA-NC组),未转染的细胞作为对照组.通过MTT法检测细胞活力,Transwell实验进行迁移和侵袭分析.通过qRT-PCR或Western blot检测细胞中FLNC、Eotaxin-1、CCR3、p-ERK1/2、t-ERK1/2和MMP-3的表达.结果 与癌旁组织相比,前列腺癌组织中FLNC的染色评分升高(1.43±0.86 vs 5.63±2.91,P<0.001).FLNC低表达组和高表达组患者的TNM分期、淋巴结转移和生存时间差异有统计学意义(P<0.05).与对照组相比,siRNA-FLNC组PC-3细胞中FLNC的mRNA和蛋白水平均降低(P<0.05),细胞活力降低(P<0.05),细胞迁移和侵袭数量降低(P<0.05).与对照组相比,siRNA-FLNC组Eotaxin-1、CCR3、p-ERK1/2和MMP-3的蛋白相对表达量均降低(P<0.05),而t-ERK1/2的蛋白相对表达量无明显变化(P>0.05).结论 FLNC在前列腺癌组织中高表达并且与患者预后相关.沉默FLNC可能通过抑制Eotaxin-1-CCR3-ERK1/2-MMP-3通路降低前列腺癌细胞的迁移和侵袭能力.

目的:总结8例机器人辅助3D打印PEEK血管外支架植入术治疗胡桃夹综合征的手术配合经验.方法:回顾性分析8例机器人辅助3D打印PEEK血管外支架植入术的术前准备、术中配合及术后注意事项.结果:8例患者手术均顺利完成,手术时间为(90±10)min,手术出血量为(40±10)ml,术后未出现相关并发症,医护配合满意度、患者满意度良好.术后3个月随访CT示左肾静脉支架位置无移位,疾病相关症状恢复良好.?结论:新技术的开展促使了手术配合模式的转变,给护理团队的配合提出了更高的要求.充分的术前准备、专业培训的护理团队、熟练的技能配合,以及合理的空间、时间管理是手术安全、顺利、有效的重要环节.

Objective:To investigate the expression of cyclin-dependent kinase 12 (CDK12) protein in prostate cancer (PCa) cells and its relationship with clinicopathological characteristics.Methods:From January 2015 to October 2016, 89 PCa patients underwent radical resection of prostate cancer and 50 benign prostate hyperplasia (BPH) underwent transurethral resection of the prostate in our hospital were enrolled. The expression of CDK12 in two pathological specimens was detected by immunohistochemistry (IHC) staining, and the relationship between CDK12 protein and clinicopathological characteristics of PCa patients was analyzed. After the operation, 89 PCa patients were followed up for 3 years, and were divided into survival group and death group according to the survival status. COX single factor and multifactor analysis were performed to summarize the risk factors affecting the prognosis of PCa patients. According to the CDK12 protein expression results, 89 PCa patients were divided into positive and negative groups, and survival curves were drawn to compare the 3-year survival status of two groups.Results:The positive expression rate of CDK12 protein in PCa tissue was significantly higher than that in BPH tissue ( P<0.05). The positive expression level of CDK12 protein had no significant difference among PCa patients with different ages ( P<0.05), while had significant difference among PCa patients with different prostate specific antigen (PSA) levels, Gleason scores and tumor stages ( P<0.05). Correlation analysis indicated that PSA level, Gleason score and tumor stage of PCa patients were positively correlated with positive CDK12 protein expression ( P<0.05). Three-year telephone and out-patient follow-up review showed that 33 PCa patients died and 56 survived. COX univariate analysis showed that there were significant differences between the survival group and the death group in PSA, Gleason score, tumor stage, and CDK12 protein expression status ( P<0.05). COX multivariate analysis indicated that PSA level>20 μg/L, Gleason score>7, tumor stage of T3, and positive expression of CDK12 protein were all independent risk factors that affected the prognosis of PCa patients. Survival curve found that 3-year survival rate of patients with CDK12 positive expression was significantly different from those of negative expression ( χ2=5.912, P<0.05). Conclusions:Over-expression of CDK12 protein may play an important role in the occurrence and development of PCa, and its positive expression level may be positively correlated with the degree of disease malignancy. Therefore, the study of CDK12 positive expression level has potential value in the assessment of the risk of PCa progression and the formulation of clinical treatment plans. Moreover, CDK12 expression is also a key factor affecting the prognosis of PCa patients.

目的 比较经腹腔与经腹膜外途径腹腔镜下寡转移前列腺癌根治术的效果和安全性.方法 回顾性分析空军军医大学第二附属医院2016年1月至2019年10月接受前列腺癌根治术的80例寡转移前列腺癌患者的临床资料,根据手术入路不同,将经腹膜外途径入路者纳入观察组(40例),将经腹腔途径入路者纳入对照组(40例).比较两组患者手术时间、术中出血量、术后导尿管留置时间、术后进食时间、术后住院天数以及并发症发生情况.结果 所有患者均顺利完成手术,两组患者手术时间、术中出血量、输血比例、并发症发生率比较差异均无统计学意义(均P>0.05);观察组患者术后进食时间、术后住院天数、术后导尿管留置时间均显著短于对照组(均P<0.05).结论 经腹膜外与经腹腔途径腹腔镜寡转移前列腺癌根治术均安全有效,但经腹膜外途径能够缩短患者术后进食时间、术后住院天数以及术后导尿管留置时间.

目的 探究miR-147a在膀胱癌组织和细胞中的表达及对膀胱癌细胞增殖及细胞侵袭能力的影响.方法 通过实时荧光定量PCR(qRT PCR)法检测空军军医大学第二附属医院检验科2020年1～12月期间采集到的30例膀胱癌组织细胞与正常组织细胞中miR-147a表达水平,分别将miR-147a mimics序列和miR NC序列转染至人膀胱移行细胞癌细胞系um-uc-3,作为miR-147a mimics组和miR NC组,通过Westernblot实验检测转染效率,采用MMT实验和Transwell实验检测并比较两组um-uc-3细胞增殖、迁移以及侵袭能力.结果 膀胱癌组织和细胞的miR-147a表达水平分别为(0.37±0.06)％、(0.41±0.08)％,明显低于正常膀胱组织的(1.20±0.15)％和细胞的(1.69±0.25)％,差异均有统计学意义(P<0.05);miR-147a mimics组转染后72 h、96 h的OD值分别为(0.75±0.26)％、(1.30±0.38)％,明显低于miR-NC组的(1.06±0.34)％、(1.71±0.46)％,差异均有统计学意义(P<0.05);miR-147a mimics组的细胞迁移数、细胞侵袭数分别为(123.06±25.16)％、(91.48±16.74)％,明显低于miR-NC组的(314.75±68.52)％、(252.30±57.23)％,差异均有统计学意义(P<0.05).结论 在膀胱癌组织和细胞中,miR-147a的表达均有不同程度的下调,体外上调miR-147a表达可有效抑制膀胱癌细胞的增殖、迁移和侵袭能力.

目的 探讨前列腺病理大切片技术的临床应用及价值.方法 回顾性分析我院行根治性前列腺切除术的155例患者的临床资料.所有患者术后均进行病理切片分析,根据病理切片方式将其分为大切片组(45例)和常规切片组(110例).比较2组患者切片情况、术后病理特征及Gleason评分.结果 2组切片均未见收缩或褶皱,结构完整,细胞形态清晰可见.其中大切片组可多层、全面地观察到前列腺病变情况,准确定位病灶,直观辨别微小病灶、前列腺切缘是否为阳性;常规切片组无法全面观察到前列腺病变情况及其与周围组织的关系,难以明确肿瘤与切缘的距离和切缘是否为阳性.大切片组切缘阳性、微小病灶、精囊侵犯检出率高于常规切片组(P<0.05),但2组病理分期检出率比较,差异无统计学意义(P>0.05).2组手术前后Gleason评分比较,差异无统计学意义(P>0.05),但大切片组术后Gleason评分升高比例高于常规切片组(P<0.05).结论 前列腺病理大切片技术可以全面观察到前列腺组织,提高切缘阳性、微小病灶及精囊侵犯检出率,为前列腺癌患者术后局部精准治疗提供病理依据.

目的 分析机器人膀胱根治性切除回盲肠原位新膀胱重建治疗肌层浸润性膀胱癌的疗效.方法 2018年1月至2019年12月,我科对9例肌层浸润性膀胱癌患者行达芬奇机器人膀胱根治性切除术,并行原位回盲肠新膀胱重建术.手术方法:机器人膀胱全切并淋巴结清扫,对尿道残端进行4针预缝合,再于下腹部切口取回肠15 cm、回盲部及升结肠12 cm作为新膀胱,颈部与预缝合的尿道吻合形成原位新膀胱.结果 手术均顺利完成,手术时间330～400 min,失血量400～800 mL.术后住院时间14～20 d.随访6～24个月,1例术后3个月出现吻合口狭窄,于膀胱镜下行尿道扩张后缓解,术后6个月复查尿路造影(C T U)均无上尿路积水,日间控尿满意.残余尿平均(50±10)mL,新膀胱容量平均为(400±50)mL,充盈期膀胱内压平均(18±2)cm H 2 O,逆行造影检查未见明显输尿管反流.结论 肌层浸润性膀胱癌患者行机器人根治性膀胱切除后,在机器人辅助下预先缝合尿道,行开放式尿流改道术时既方便操作又缩短了手术时间.回盲肠原位新膀胱保留了肠道管状结构,不仅能低压储尿而且可依靠肠管的收缩力达到高压时克服尿道括约肌阻力从而排尿,尿控效果满意,逆行感染、尿失禁、残余尿等并发症发生较少,是一种值得推荐的尿流改道选择.

目的:探讨逆行全膀胱切除术后原位回盲肠新膀胱术的手术方式、近期疗效和尿流动力学特点.方法:回顾性分析2018年11月至2019年8月我科收治并行原位回盲肠新膀胱术膀胱癌患者4例,所有患者先行腹膜外逆行根治性全膀胱切除,截取回盲肠构建新膀胱,再将新膀胱与尿道吻合重建尿流通道.术后定期复查尿动力、肾功能、彩超等检查.结果:本组患者随访6～16个月,术后初期患者均有不同程度溢尿现象,3个月后逐步恢复并能良好控尿.1例术后出现尿道吻合口轻度狭窄并输尿管返流.新膀胱最大储尿容量(401.7±53.0)ml,储尿期膀胱内压(19.0±5.7)cmH2 O,尿道闭合压(53.6±9.4)cmH2 O,储尿期膀胱内压明显小于尿道闭合压,最大尿流率(18.7±1.5)ml/s,平均残余尿量(21.3±4.4)ml.结论:全膀胱切除术后原位回盲肠新膀胱术具有储尿囊容量大、压力低、可控性好、操作简单的优点,是一种较理想的尿流改道方式.

Abstract Background: The Prostate Cancer Prevention Trial has shown a protective effect of finasteride on prostate cancer, but it also showed that finasteride can increase the risk of high-grade prostate cancer. Several studies have investigated the relationship between finasteride and prostate cancer, but these studies have shown inconsistent results. Ethics: The protocol was approved by the institutional review board of each study center. Written informed consent will be obtained from all patients before registration, in accordance with the Declaration of Helsinki. Methods: We performed a systematic literature review and meta-analysis to assess the association between finasteride and prostate cancer. Systematic literature searches were conducted using PubMed, EMBASE, Science Direct/Elsevier, MEDLINE, CNKI, and the Cochrane Library up to October 2018 to identify studies that involved the relationship between finasteride and prostate cancer. Meta-analysis was performed using Review Manager and Stata software. Combined ORs were identified with 95% confidence intervals (95% CI) in a random or fixed effects model. Results: Eight studies were identified, including 54,335 cases of patients that used finasteride and 9197 patients who served as placebo controls. Our results illustrate that there is a significant correlation between finasteride use and prostate cancer with combined ORs of 0.70 [0.51, 0.96]. A significant correlation between finasteride use and high-grade prostate cancer was also observed with combined ORs of 2.10 [1.85, 2.38]. Conclusions: This study confirms that finasteride significantly reduced the risk of prostate cancer; however, the malignant degree of prostate cancer was increased. Studies with larger sample sizes are needed to better clarify the correlation between finasteride use and prostate cancer.

Abstract Background Sex-determining region Y-box containing gene 30 (SOX30) is a newly identified tumor-associated gene in several types of cancer. However, whether SOX30 is involved in the development and progression of prostate cancer remains unknown. This study investigated the potential role of SOX30 in prostate cancer. Methods Prostate cancer cell lines and a normal prostate epithelial cell line were used for the experiments. The expression of SOX30 was determined using quantitative real-time PCR and western blot analysis. The malignant cellular behaviors of prostate cancer were assessed using the Cell Counting Kit-8, colony formation and Matrigel invasion assays. The miRNA–mRNA interaction was validated using the dual-luciferase reporter assay. Results SOX30 expression was lower in cells of prostate cancer lines than in cells of the normal prostate epithelial line. Its overexpression repressed the proliferation and invasion of prostate cancer cells. SOX30 was identified as a target gene of microRNA-653-5p (miR-653-5p), which is upregulated in prostate cancer tissues. MiR-653-5p overexpression decreased SOX30 expression, while its inhibition increased SOX30 expression in prostate cancer cells. MiR-653-5p inhibition also markedly restricted prostate cancer cell proliferation and invasion. SOX30 overexpression or miR-653-5p inhibition significantly reduced β-catenin expression and downregulated the activation of Wnt/β-catenin signaling. SOX30 knockdown significantly reversed the miR-653-5p inhibition-mediated inhibitory effect on the proliferation, invasion and Wnt/β-catenin signaling in prostate cancer cells. Conclusions These results reveal a tumor suppressive function for SOX30 in prostate cancer and confirmed the gene as a target of miR-653-5p. SOX30 upregulation due to miR-653-5p inhibition restricted the proliferation and invasion of prostate cancer cells, and this was associated with Wnt/β-catenin signaling suppression. These findings highlight the importance of the miR-653-5p–SOX30–Wnt/β-catenin signaling axis in prostate cancer progression.