Objective:To evaluate the association between rs2294008 C＞T polymorphism of prostate stem cell antigen (PSCA) gene and the risk of gastric cancer in East Asian by using Metaanalysis.Methods:A computer-based online search was performed in PubMed,Web of Science,Cochrane Library,Google Scholar,China National Knowledge Infrastructure,Chinese BioMedical Literature database,VlP database and Wanfang database.The case-control studies about the association between PSCA rs2294008C＞T polymorphism and gastric cancer in East Asian before June 6,2016 were collected.The literatures were screened according to the inclusion and exclusion criteria,and the data were extracted after the quality analysis.The Meta-analysis was performed by STATA 14.1 software,and the odds ratio (OR) and 95％ confidence interval (CI) were calculated.Furthermore,the subgroup analysis and Metaregression analysis were used to evaluate the confounding factors,and the sensitivity analysis and publication bias test were performed.Results:A total of 13 case-control studies contained 13 667 patients and 11 868 health controls were included.Meta-analysis showed that the carriers with PSCA rs2294008 CT and TT genotypes had significantly higher risk of gastric cancer as compared with other genotype (OR =1.68,95％ CI:1.43-1.96).The polymorphism distribution of PSCA rs2294008 was significantly different among Chinese,Japanese and Korean populations (P =0.001).Subgroup analyses showed that the risk of gastric cancer on the carriers with PSCA rs2294008CT and TT was significantly increased in Chinese (OR =1.36,95％ CI:1.23-1.49,P =0.004),Japanese (OR =2.61,95％ CI:2.22-3.07,P =0.001) and Korean populations (OR =2.04,95％ CI:1.28-3.27,P =0.012) compared with rs2294008 CC.The sub-group analyses also showed that there was no significant difference in the risk of gastric cancer between the controls from hospitals (OR =1.74,95％ CI:1.23-2.44) and ones from communities (OR =1.64,95％ CI:1.40-1.93).The Meta-regression found the differences in countries and control sources were not the major contribution to the heterogeneity in this analysis (P =0.163,P =0.381).The Begg's funnel plots showed no publication bias (corrected P =0.053),and the sensitivity analysis confirmed the data were steady (OR =1.54,95％ CI:1.45-1.64).Conclusion:The polymorphism of PSCA rs2294008 C＞T is closely associated with the risk of gastric cancer in East Asia.

Objective To explore the role of Par6 in the development of glioma by analysis of the expression of Par6 in glioma tissue. Methods 70 cases of human glioma tissue with different clinical period and differentiation histological type and 20 cases of normal brain tissue were selected.The protein of Par6 was detected by immunohistochemistry. Results Compared with normal brain tissue,the expression of Par6 protein in glioma tissue decreased significantly (P<0. 05).The expression level of Par6 was lower at stageⅢ~Ⅳthan that atⅠ~Ⅱ(Ⅰ~ⅡvsⅢ~Ⅳ;P=0.008) . Conclusion The expression of Par6 in glioma tissue decreased,and Par6 was involved in the development and progression of glioma,which was markedly correlated with clinical stage.

OBJECTIVE To evaluate the effects of atorvastatin on adipocyte fatty acid binding protein(AFABP)secretion and expression induced by oxidized low density lipoprotein(OxLDL)in adipocytes.METHODS Subcutaneous adipose tissues were collected from normal rabbits for adipocytes culture.The adipocytes were exposed to OxLDL and atorvastatin at different concentrations.AFABP concentrations in adipocytes culture supernatant were measured by ELISA.The levels of AFABP and PPARγ mRNA in adipocytes were measured by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)studies.RFSULTS The OxLDL in low concentrations stimulated adipocytes release of AFABP;furthermore,OxLDL increased AFABP and PPARγ,mRNA expressions of adipocytes in a dose-dependent manner.The treatment of adipocytes with atorvastatin ihhibited AFABP mRNA expression induced by OxLDL and reduced release of AFABP in adipocytes with a dosedependent manner.CONCLUSION These findings indicated that atorvastatin may inhibit AFABP expression and secretion induced by OxLDL in adipocytes which might be of significance for the prevention and treatment of atherosclerosis.

对106例各种肝脏疾病合并腹泻的住院病人资料进行总结和分析。结果:(1)各种肝脏疾病中,肝源性腹泻发生率由高到低依次为:各型肝硬化(64.15%)、慢性乙型病毒性肝炎(18.87%)、原发性肝癌(11.32%)、脂肪肝(3.77%)和药物性肝病(1.89%),其临床表现无特征性;(2)腹泻发生在肝病早期的较少,大多出现在肝病的中后期;(3)腹泻的严重程度与肝功能密切相关。早期诊断和改善肝功能是治疗肝源性腹泻的关键。

目的观察非诺贝特干预对高脂血症兔脂肪组织和细胞清道夫受体BI(SR-BI)表达的影响.方法 10只新西兰大白兔给予高胆固醇饮食饲养8周后,随机分为2组:⑴高胆固醇组:继续饲以高胆固醇饲料4周;⑵非诺贝特组:在饲以高胆固醇饲料的基础上给予非诺贝特(30 mg/kg/d),共4周.另选兔(n＝5)普通饮食12周作为对照组.取腹股沟皮下脂肪组织行脂肪细胞培养.半定量逆转录多聚酶链式反应(RT-PCR)测定脂肪组织和细胞SRBI mRNA的表达.结果非诺贝特治疗组和高胆固醇组血清总胆固醇、低密度脂蛋白胆固醇水平均明显高于对照组(P＜0.001),但2组间差异无统计学意义 (P＞0.05).高胆固醇组兔脂肪组织和细胞SRBI基因表达水平较正常组增加(P＜0.05),非诺贝特组SRBI基因表达水平高于高胆固醇组(P＜0.05).结论非诺贝特能上调高脂血症兔脂肪组织和细胞SRBI mRNA表达, 提示非诺贝特可能参与脂肪细胞胆固醇代谢的调节.

Objective To investigate the relationship between the p33 ING1b protein expression and differentiated degree in human gastric carcinoma. Methods p33 ING1b protein expression was detected by S-P immunohistochemical method in gastric carcinoma and normal gastric mucous tissues. Results The positive rates of p33 ING1b protein expression were 75% (15/20) in well-differentiated, 55% (11/20) in moderate-differentiated and 15% (3/20) in poor-differentiated gastric carcinoma, respectively. The positive rate of p33 ING1b protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa (100%, 60/60) (P0.05). The p33 ING1b expression was positively related with differentiated degree of gastric carcinoma (P0.05). Conclusion There was a loss expression of p33 ING1b protein in gastric carcinoma. The loss expression of p33 ING1b protein was related to differentiated degree of gastric carcinoma.

Objective To investigate whether urinary trypsin inhibitor inhibits tumor invasion and metastasis of lewis lung carcinoma mice .Methods Subcutaneous (s.c.) implantation of 3LL cells(~5.0 ×10~6 ) in the right subcutaneous armpit of C57BL/6 male mice.There were forty mice divided into five groups with random.There were physiological saline group, cyclophosphamide group , UTI2.5 10~4 u 7d group ,UTI~5.0 ×10~4 u 7d group,UTI~10.0 ×10~4 u 7d group. They all started to inject at sixth day by abdominal cavity . The volume of tumors were measured at the 8th day ,10 th day ,12 th day . The weight of primary tumor and lung metastasis were established by the 14 th day after tumor cell inoculation . Flow Cytometry was used to analyze apoptosis rate and s-phase fraction. Results The average weight of tumor in turn were(~7.92 ±~2.52 、~0.66 ±~0.50 ~** 、~3.47 ±~1.45 *、~3.08 ±~0.81 ~** 、~1.70 ±~1.05 ~** )g, the average number of lung metastasis were (~8.625 ±~1.407 、~1.125 ±~1.126 ~** 、~1.625 ±~1.302 ~** 、~1.00 ±~0.75 ~** 、~0.625 ±~0.74 ~** ).The apoptosis rates of CTX (~39.3 ±~4.8)% * and UTI~10.0 ×10~4 u (~40.2 ±~3.1)% * were markedly increased. The s-phase fractions of UTI cannot reduce s-phase fraction. The tumor growth curve showed in FIG.3.Conclusion Ulinastatin can inhibit primary tumors and lung metastasis carcinoma of Lewis mice.

Objective To investigate the expression of S100A2 protein and analyze its relationship between differentiation of gastric carcinoma. Methods The expression of S100A2 protein was detected with streptavidin-peroxidase conjugated (S-P) immunohistochemical technique method in 40 cases of gastric carcinoma and normal gastric mucosa. Results The positive rates of S100A2 protein expression were 52.50% in gastric carcinoma and 100% in normal gastric mucosa respectively. The positive rate of S100A2 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa (P0.05). The positive rate of S100A2 expression were 75.00% in highly, 53.84% in moderately and 18.18% in poorly differentiated gastric carcinoma respectively, which indicated the expression of S100A2 protein was related to the degree of differentiation of gastric carcinoma. Conclusions The loss expression of S100A2 protein was in gastric carcinoma. The positive rate of S100A2 protein expression in poorly differentiation of gastric carcinoma was lower than that in highly and moderately differentiated gastric carcinoma, which indicated that the loss of expression of S100A2 protein was related to the carcinogenesis and differentiation of gastric carcinoma. S100A2 gene might play an important role in carcinogenesis of gastric carcinoma.

目的观察5-FU+CF的新辅助化疗方案在局部进展期胃癌中的运用.方法 32例局部进展期(中晚期)胃癌病人行5-FU+CF方案的新辅助化疗,并观察临床症状和体征变化、病理变化,并有16例患者观察了影像学变化.结果临床改善率78.1%,组织病理学有效率68.8%,影像学肿块有效缩小率43.8%,肿块R0切除率71.9%.结论胃癌的新辅助化疗能提高肿瘤R0切除率,改善预后,提高肿瘤综合治疗效果,5-FU+CF手术前化疗方案值得推广.

目的:探讨糖尿病新西兰兔心、肾一氧化氮合酶(NOS)活力和抗氧化酶活力变化以及它们在糖尿病发病机制中的作用.方法:采用高脂高糖饲料喂养新西兰兔,建立一种新的糖尿病动物模型.观察糖尿病新西兰兔心、肾一氧化氮水平,一氧化氮合酶(NOS)活力及抗氧化酶活力的变化.结果:糖尿病新西兰兔出现大量蛋白尿(P＜0.05);肌酐清除率(CCr)明显高于对照组(P＜0.01);心脏组织中NO-2水平和NOS活力明显低于对照组(P＜0.05),而肾脏组织中NO-2水平和NOS活力明显高于对照组(P＜0.01);血糖及胰岛素明显高于对照组(P＜0.01);超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(catalase)活性都明显低于对照组(P＜0.01).结论:糖尿病新西兰兔心、肾中的NO-2水平和NOS活力异常,抗氧化酶活力明显降低,糖尿病的心、肾并发症可能与它们的异常变化有关.

目的:探讨糖尿病新西兰兔一氧化氮(NO)水平和抗氧化酶活力变化以及它们在糖尿病发病机制中的作用.方法:采用高脂高糖饲料喂养新西兰兔,建立糖尿病动物模型.观察糖尿病新西兰兔血液中的一氧化氮水平,一氧化氮合酶(NOS)活力及抗氧化酶活力的变化.结果:糖尿病新西兰兔血浆中NO水平和NOS活力明显增高,抗氧化酶活性低于喂正常饲料新西兰兔.结论:糖尿病的起因可能与血浆中NO水平和抗氧化功能的异常变化有关.

Objective: To establish diabetic model in New Zealand rabbits and study the relationship between nitric oxide(NO) pathway and diabetic nephropathy.Methods:Two groups of New Zealand White rabbits received regular rabbit chow (the normal control) or high fat high sucrose diet for 4 months. The levels of plasma insulin and glucose were investigated. The level of renal NO and the activity of renal NO synthase (NOS) were measured besides morphological observation. Results:Glomerular lesions in test group involved mesangial cell proliferation trend of K-W node and fibrin cap and accumulation of mesangial matrix and thickness of glomerular membrane basement.There were still wall thickness and cavity stricture in renal resistant vasculature.Renal NO level and NOS activity were higher in test group than in control group(P0.001). There was an elevation of the kidney/body weight in the early stage of the diabetes(P0.05)as well as a great amount of proteinuria(P0.05).The creatinine clearancerate(CCr) increased apparently(P0.001). Conclusion:New Zealand White rabbits could be used to establish the model of diabetes; Nitric oxide might be the main inductive mediator of the glomerular hyperfiltration in early diabetes.