用生物信息学方法筛选肺腺癌(Lung adenocarcinoma,LUAD)的诊断生物标志物,并分析肺腺癌中免疫细胞浸润情况.从GEO和TCGA数据库下载肺腺癌的表达数据集,利用R软件筛选肺腺癌与正常肺组织间的差异表达基因(DEGs),使用DAVID网站对DEGs进行GO及KEGG富集分析,使用STRING及Cytoscape等工具对DEGs构建蛋白相互作用网络并筛选hub基因;利用Kaplan-Meier法对DEGs进行生存分析,并对hub基因进行ROC分析筛选诊断生物标志物,利用GSEA预测有预后价值的基因参与的信号通路;并用Cibersort软件反卷积算法分析肺腺癌中免疫细胞浸润情况.共得到肺腺癌的234个DEGs,这些基因主要参与信号转导、物质代谢、免疫反应等相关信号通路;构建PPI网络筛选出的20个hub基因中8个存在预后价值(CCNA2、DLGAP5、HMMR、MMP1、MMP9、MMP13、SPP1、TOP2A),ROC分析中DLGAP5、SPP1值分别是0.703、0.706;DLGAP5、SPP1基因表达水平与肺腺癌组织浆细胞、未活化的CD4+记忆细胞、调节T细胞、巨噬细胞M0、M1、M2及中性粒细胞浸润密切相关(P<0.05).肺腺癌中DLGAP5、SPP1具有较高诊断价值且参与肺腺癌组织免疫细胞浸润;DLGAP5、SPP1基因可作为肺腺癌诊断的生物标志物,可为肺腺癌的靶向治疗研究提供新思路.

肺癌严重危害人类健康,患者预后较差.肺纤维化是肺癌的危险因素之一,与肺癌的发病机制存在一定关系.肺纤维化中失调的微小RNA(microRNA,miRNA)在肺癌发生发展过程中发挥着重要作用.本研究使用基因表达综合数据库(Gene Expression Omnibus,GEO)中肺纤维化miRNA及肺癌mRNA的芯片数据,利用GEO2R筛选肺纤维化差异miRNA与肺癌差异基因、miRWalk预测miRNA靶基因、STRING分析互作关系、WebGestalt进行GO与KEGG分析、GEPIA分析基因对患者生存的影响,最终获得SNRPE、PIK3R1、ARHGEF6等潜在的肺癌治疗靶点.通过对这些潜在靶点的认识进一步了解肺癌的发生发展机制,为肺癌的临床治疗贡献新的思路.

近年来发现环状RNA(circular RNA,circRNA)在各种类型的上皮间质转化(epithelial-mesenchymal transition,EMT)中发挥了重要的作用.本文就EMT的概念和分类、circRNA的分类和生成,以及circRNA分别在EMT过程中作用的国内外研究进展作一综述,以期为发生EMT的器官纤维化、肿瘤等疾病的靶向治疗和预防寻找新的研究方向.

常用的呼吸道染毒方法有暴露式气管滴注、鼻内滴注和非暴露式气管滴注.非暴露式气管滴注通过气管插管将滴注液输送到肺部,无需手术切口暴露气管,染毒剂量准确且对实验动物伤害较小.非暴露式气管滴注的方法有:盲探插管、透射光插管,使用光纤、喉镜、头戴式放大镜、内窥镜等辅助插管,但这些方法大多需要特殊设备,对于大鼠等小型哺乳类动物而言,由于其口腔、咽部解剖学情况使得插管较为困难,现今还没有标准的气管插管设备供大鼠使用,且操作技能要求较高[1-7].本课题组拟建立一种操作简单、安全性高、动物死亡率低、设备要求低的大鼠非暴露式气管滴注方法及其评价方法,现介绍如下.

随着中国综合国力的迅猛提升及中国医学事业的瞩目发展,我国高等医学院校吸引了众多第三世界国家的学子来华学医. 2011年,我校迎来了第一批巴基斯坦、阿拉伯、南非籍医学留学生,是我校首次招收的医学本科留学生,成为我校特色办学的一个亮点,医学留学生教育工作对我校本身办学能力和教学水平带来很大的挑战. 留学生教育质量反映了学校国际化教育水平,培养的留学生将进入各国医学领域,他们的职业素养会体现出学校的国际声誉.

目的 探究肺癌化疗患者认知功能状况,并分析其影响因素,以期制订合理有效的干预措施,为临床提供参考.方法 本研究采用“整群抽样法”,选取2018年11月1日～2019年4月30衡阳市4所三级甲等医院肺癌化疗患者300例作为研究对象,采用一般资料调查表、简明精神状态量表(MMSE)进行调查.分析肺癌化疗患者的认知能力及认知水平,并研究其影响因素.结果 肺癌化疗患者MMSE测试平均分(25.530±3.283)分,认知水平低下者41例(14.4％).单因素分析结果显示年龄、婚姻状况、教育程度、职业、医疗支付形式、是否锻炼身体、对疾病了解程度、TNM分期、化疗周期及是否转移对肺癌化疗患者认知功能有影响(P＜0.05).多元线性逐步回归分析结果显示,年龄、婚姻状况、教育程度、身体锻炼、卡氏评分和化疗周期是肺癌化疗患者认知功能的主要影响因素(P＜0.05).结论 肺癌化疗患者中存在部分认知水平低下的患者且其认知功能受多重因素影响,开展认知功能测评,有助于医护人员了解患者的认知功能情况,有利于制订有效的干预措施,帮助患者改善其认知功能.

目的 探讨肺癌化疗患者疲乏-负性情绪-疼痛-睡眠障碍症状群及其影响因素.方法 2017年9-12月,便利抽样法抽取湖南省五所三级甲等医院住院治疗的肺癌化疗患者220名为研究对象.采用一般资料调查表、数字疼痛量表、简易疲乏量表、医用焦虑抑郁量表和匹兹堡睡眠质量指数量表对其进行调查,同时收集其化疗期间血液检测结果.采用聚类分析对疲乏-负性情绪-疼痛-睡眠障碍症状群进行分组,多元线性逐步回归分析筛选其影响因素.结果 通过聚类分析得出,肺癌化疗患者疲乏-负性情绪-疼痛-睡眠障碍症状群分为重度和轻度两个症状组,其中化疗周期、患病时长、白细胞、血小板和谷丙转氨酶水平是其主要影响因素(均P＜0.05).结论 化疗周期、患病时长、血小板、白细胞和谷丙转氨酶与症状群严重程度密切相关.医护人员对于化疗周期短、患病时间较长及白细胞、血小板和谷丙转氨酶异常的患者应加强观察,及时评估,并采取针对性的预防及症状管理措施.

目的 探讨肺癌化疗患者症状群现况及其与临床常见血液指标的相关性,为肺癌化疗患者症状群的评估及制定预防和护理措施提供参考依据.方法 便利抽样法抽取2017年10月至2018年4月湖南省三所三级甲等医院住院治疗的肺癌化疗患者210名,采用自制一般资料调查表、自制疾病相关资料调查表、安德森症状量表对196名肺癌化疗患者进行了有效问卷调查.采用因子分析法、Pearson相关分析研究肺癌化疗患者症状群严重程度与血液检测结果的相关性.结果 肺癌化疗患者存在"情绪相关症状群""病感症状群""肺癌特异性症状群"三组症状群,且大部分患者三组症状群的严重程度处于中等水平;其中,情绪相关症状群与镁离子浓度呈负相关(P=0.001,r=-0.239),病感症状群与白细胞数(P<0.001,r=-0.474)和血小板数(P<0.001,r=-0.346)成显著负相关,肺癌特异性症状群与凝血酶原时间(P<0.001,r=0.336)、纤维蛋白原浓度成显著正相关(P<0.001,r=0.319),而与白蛋白(P<0.001,r=-0.273)、总蛋白(P<0.001,r=-0.270)浓度呈显著负相关(P<0.05).结论 肺癌化疗患者存在"情绪相关症状群""病感症状群""肺癌特异性症状群"三组症状群,且其严重程度与血液检测结果密切相关;医务工作者可通过血液检测了解症状群的发生和变化情况,及时采取有效干预措施,降低患者症状负担.

目的 探讨第二课堂建设在临床医学见习实践教学中的应用效果.方法 选择南华大学附属第二医院2016级临床医学专业72名学生作为研究对象,分为观察组和对照组,每组36名.其中观察组学生采用以问题为基础的学习法(PBL)第二课堂教学法进行教学;对照组学生采用传统第一课堂教学法模式进行教学.结果 观察组学生出科平均考试成绩[(88.7±3.04)分]高于对照组[(81.9±2.01)分],差异有统计学意义(P＜0.05).通过问卷调查发现,观察组各项评价指标均明显优于对照组,差异均有统计学意义(P＜0.05).结论 PBL式第二课堂教学法在临床医学见习实践教学中可行性强,可以显著提高学生专业理论水平及临床实践能力,为培养医学基础理论知识、临床技能、医学人文素质三位一体的新型医学人才创建了一个开发潜能、施展才华的平台.

目的 探讨内源性硫化氢(H2S)对硫酸铍(BeSO4)诱导人支气管上皮细胞(16HBE)自噬的影响.方法 用0、100、150、200、250和300 μmol/L BeSO4处理16HBE细胞48 h,MTT法测定细胞活力,亚甲基蓝分光光度法测定细胞内H2S含量,Western blot测定自噬标志蛋白LC3-B、Beclin-1及P62表达;用300 μmol/L硫氢化钠(NaHS)或10 mmol/LDL-炔丙基甘氨酸(PPG)预处理16HBE细胞6h后,用BeSO4处理细胞,研究H2S含量对上述指标的影响.结果 (1)BeSO4作用16HBE细胞48 h的半数致死浓度为249 μmol/L;与对照组相比,BeSO4组自噬蛋白LC3-Ⅱ/LC3-Ⅰ和Beclin-1表达升高,细胞活力、H2S含量和P62蛋白表达降低,当BeSO4浓度＞100 μmol/L时,差异均有统计学意义(P＜0.05);(2)与BeSO4组比较,NaHS+BeSO4组细胞活力、H2S水平和P62蛋白表达均升高(P＜0.05),LC3-Ⅱ/LC3-Ⅰ和Beclin-1蛋白表达有降低趋势,但差异无统计学意义;(3)与BeSO4组比较,PPG+BeSO4组细胞活力、H2S水平及P62蛋白表达降低,LC3-Ⅱ/LC3-Ⅰ和Beclin-1蛋白表达升高(P＜0.05).结论 在本试验条件下,BeSO4染毒能致16HBE细胞活力和内源性H2S含量下降,并引起细胞自噬;内源性H2S能抑制BeSO4诱导的16HBE细胞自噬、减轻细胞毒性,发挥保护功能.

Objective To evaluate the mitomycin C (MMC) treatment on the miRNA expression profile in gastric cancer cells,and provide the theoretical evidences for MMC treatment on gastric cancer.Methods The effects and optimum concentration of MMC were evaluated in the cultured SGC-7901 cells by MMT.The miRNA microarray technique was used for the exploration of miRNA profile of SGC-7901 cells after MMC treatment and the different expression of candidate miRNA was further confirmed by qRT-PCR.Results The inhibition rate of SGC-7901 cells was negatively correlated with the MMC concentration.The 60 miRNAs have changed expression in SGC-7901 cells 48 hours after MMC treatment.Among them,11 miRNAs included miR-498 and miR-1247 were up-regulated,and miR-205* and miR-3924 were down-regulated.Some abnormal expression changes recovered after MMC treatment.Conclusion MMC can regulate the expression of 60 miRNAs including miR-498 in gastric cancer cells.

Objective:To evaluate the association between rs2294008 C＞T polymorphism of prostate stem cell antigen (PSCA) gene and the risk of gastric cancer in East Asian by using Metaanalysis.Methods:A computer-based online search was performed in PubMed,Web of Science,Cochrane Library,Google Scholar,China National Knowledge Infrastructure,Chinese BioMedical Literature database,VlP database and Wanfang database.The case-control studies about the association between PSCA rs2294008C＞T polymorphism and gastric cancer in East Asian before June 6,2016 were collected.The literatures were screened according to the inclusion and exclusion criteria,and the data were extracted after the quality analysis.The Meta-analysis was performed by STATA 14.1 software,and the odds ratio (OR) and 95％ confidence interval (CI) were calculated.Furthermore,the subgroup analysis and Metaregression analysis were used to evaluate the confounding factors,and the sensitivity analysis and publication bias test were performed.Results:A total of 13 case-control studies contained 13 667 patients and 11 868 health controls were included.Meta-analysis showed that the carriers with PSCA rs2294008 CT and TT genotypes had significantly higher risk of gastric cancer as compared with other genotype (OR =1.68,95％ CI:1.43-1.96).The polymorphism distribution of PSCA rs2294008 was significantly different among Chinese,Japanese and Korean populations (P =0.001).Subgroup analyses showed that the risk of gastric cancer on the carriers with PSCA rs2294008CT and TT was significantly increased in Chinese (OR =1.36,95％ CI:1.23-1.49,P =0.004),Japanese (OR =2.61,95％ CI:2.22-3.07,P =0.001) and Korean populations (OR =2.04,95％ CI:1.28-3.27,P =0.012) compared with rs2294008 CC.The sub-group analyses also showed that there was no significant difference in the risk of gastric cancer between the controls from hospitals (OR =1.74,95％ CI:1.23-2.44) and ones from communities (OR =1.64,95％ CI:1.40-1.93).The Meta-regression found the differences in countries and control sources were not the major contribution to the heterogeneity in this analysis (P =0.163,P =0.381).The Begg's funnel plots showed no publication bias (corrected P =0.053),and the sensitivity analysis confirmed the data were steady (OR =1.54,95％ CI:1.45-1.64).Conclusion:The polymorphism of PSCA rs2294008 C＞T is closely associated with the risk of gastric cancer in East Asia.

Objective To analysis the correlation between uncertainty in illnesses and perceived control in patients with non-small-cell lung cancer (NSCLC) undergoing chemotherapy.Methods A consecutive sample of 145 patients with NSCLC were recruited and assessed with socio-demographic, medical data, the scales of the Michel′s Chinese version of uncertainty in illness and the cancer experience and efficacy scale (CEES).Results The total score of the uncertainty in illness in patients with NSCLC was (98.56±9.99) points, the total score of the cancer experience was (3.97±0.40) points, and the total score of the cancer efficacy was (3.13±0.52) points.In CEES, the scores of each dimension from the highest to the lowest were social economics [(4.25±0.59) points], emotional experience [(3.89±0.50) points], personal experience [(3.82±0.42) points], collective efficacy [(3.30±0.83) points], medical efficacy [(3.18±0.52) points], and personal efficacy [(2.93±0.76) points].The score of the uncertainty in illness was significantly positively correlated with experience subscales (r=0.291, P<0.001), and negatively correlated with efficacy subscale (r=-0.334, P<0.05).Conclusion Uncertainty in illnesses of the patients is correlated with perceived control.To raise the efficacy of patients and improve their quality of life, it is important for health-care workers to pay attention to the ability of cognition in patients.

目的：探讨内镜逆行胆胰管造影（ERCP）下胆道塑料支架及自膨式金属网眼支架置入治疗恶性梗阻性黄疸的有效性及安全性。方法回顾性分析并总结2005年3月至2014年12月收治的71例接受ERCP下胆道自膨式金属网眼支架置入和97例塑料支架置入治疗的恶性梗阻性黄疸患者的临床资料。结果162例患者支架置入成功， ERCP成功率为96.4%（162/168）；术后1周总胆红素由（325.3±96.2）μmol/L降至（147.9±65.8）μmol/L，直接胆红素由（188.5±81.6）μmol/L降至（86.5±45.3）μmol/L，二者水平均较术前显著下降（P<0.05）；患者临床症状明显改善，总有效率为96.9%（157/162）。生存期延长，最长的1例达到71个月，中位生存时间10.6个月；术后13例出现高淀粉酶血症及2例急性胰腺炎，经保守治疗全部治愈，4例术后出现胆管炎；无出血、十二指肠穿孔等其他严重并发症发生。结论 ERCP下金属网眼支架及胆道塑料支架置入对于晚期、高龄的恶性梗阻性黄疸患者是一种安全、微创、有效的治疗方法；对肝门部胆管癌患者将支架置入右或左肝管是延长生存期的重要措施。

Objective To investigate the applied value of Choledochoscopy and electrohydraulic lithotripsy( EHL) in the operation of pancreatolithiasis. Methods The clinical data of diagnosis and treatment for pancretic duct stone in 46 patients treated in the Second Affiliated Hospital of University of South China from January 2002 to April 2014 were retro-spectively analyzed,two patients underwent pancreatic coduodenectomy ( Whipple) for being combined with pancreatic head carcinoma,one patient underwent resection of pancreatic tail and spleen and pancreatic stump jejunosomy(Pusetow)20 pa-tients underwent the pancreatic duct lithotomy,dredging main pancreatic duct and pancreatic enteric anastomosis combined with Roux-en-Y,with intraoperative choledochscopy being used jointly ( Choledochoscopy combined with Operation group ) . During the period of only with the pancreatic duct lithotomy,dredge main pancreatic duct and pancreatic enteric anastomosis combined with Roux-en-Y,and the data of these patients were originated in the data of 10 patients with pancreatolithiasis ( Routine group) ,and the data of 12 patients who were treated by Endoscopic retrograde cholangiopan-Creatography ( ER-CP) combined with extracorporeal shock wave lithotripsy ( ESWL) ( ERCP+ESWL group) were compared. Results The intraoperative blood loss was more than that in the ERCP+ESWL group[(120 ± 55. 9)mL vs(30 ± 15. 4)mL,(t=3. 228,P=0. 042) ],so the difference was significant (P=0. 042),but it was significantly less than that in the Routine group[(120 ± 55. 9)mL vs (583. 0 ± 135. 4)mL,(t= 5. 446,P=0. 032)]. The postoperative rate of residual stones in the&nbsp;Choledochoscopic lithotomy group was significantly lower than that in the ERCP+ESWL group and in the Routine group (P=0. 008)The postoperative rate of pain relief was significantly higher than that in the Routine group(90% vs 50%)and in the ERCP+ESWL group(90% vs 50%). Conclusion For the surgical treatment of pancreatolithiasis,it can signifi-cantly decrease the postoperative rate of residual stones and introperative blood loss,and increase the postoperative rate of pain relief in the midsection when Choledochoscopy and extracorporeal shock wave lithotripsy are used jointly.

目的：探讨内镜下组织胶注射治疗肝硬化胃底静脉曲张出血的疗效和安全性。方法回顾分析内镜下组织胶注射治疗154例胃底静脉曲张出血患者的疗效。注射步骤采用三明治夹心法：碘化油充填注射针,然后快速注入组织胶,再接1.4 mL碘化油快速注射、拔针,组织胶不稀释,静脉团直径在1.5 cm以下一次性注射0.5~1 mL,静脉团直径1.5~3.5 cm一次注射1.5~2.0 mL即可使整个团块硬化。术后2~6个月复查胃镜,如还有残留曲张静脉再追加注射。结果在154例病例中,其中急诊组织胶注射37例急性活动性出血33例止血,即时止血率89.2‰(33/37)；治疗后镜下静脉团基本消失,占40.9‰(63/154)；显著有效占44.8‰(69/154)；有效占7.8‰(12/154)；无效占6.5‰(10/154)。显著有效率达85.7‰(132/154)。随访3~24个月,近期(6月内)再出血率11例和远期再出血率7例共占11.7‰,胃底静脉曲张程度、肝功能分级、门静脉血栓、血小板计数、胆碱酯酶、门静脉宽度在术后再出血患者和未出血患者群中分布差异显著(P均<0.05)。结论胃底静脉曲张破裂出血凶险,内镜下组织胶注射治疗是控制急性活动性出血及预防再出血快捷、安全、有效的方法。

Objective To discuss ultrasonic endoscope′s diagnostic value in upper gastrointestinal tract submucosal tu-mor (SMT) and the curative effect and safety of using endoscope minimally invasive surgery to cut off submucosal tumor. Meth-ods Did ultrasonic endoscope on 110 SMT patients who did gastroscopy in this hospital from October ,2013 to December,2014. Different treatment were used in line with test results. 50 of them did endoscopic mucosal resection (EMR),12 of them did endo-scopic submucosal dissection(ESD)(5 ESE),1 of them did submucous tunnel endoscopic resection(STER),19 of them did sur-gical removal. Did pathology and immunohistochemical test on surgical samples. Summarized ultrasonic endoscope′s characteris-tics and made a comparison with ultimate diagnosis. Postoperative endoscope follow-up at regular intervals for 1-24 months was done to observe the safety and effectivity of endoscope minimally invasive surgery. Results 110 patients were diagnosed as li-omyoma(n=29),mesenchymoma(n=39),lipomyoma(n=12),heterotopic pancreas(n=19),cyst(n=7),neuroendocrine(n=4) by endoscope ultrasonic examination. 89 excision samples manifested complete focus excision by pathological examination. There was no lesion tissue residual in incisal edge and basilar part. There were 39 mucosal muscularis lesions ,28 submucosa lesions,22 muscularis propria tumors. The accordance rate of endoscope ultrasonic diagnosis and postoperative pathology diagnosis nature was 84.27%(75/89). The accordance rate of source level was 87.64%(78/89). 2 hemorrhage and 3 perforation patients were clipped under endoscope and cured using conservative treatment. 86 patients did not relapse or have long-term complications dur-ing postoperative follow-ups after endoscope surgery. Conclusions Using ultrasonic endoscope and endoscope minimally inva-sive surgery to cut off SMT is a safe and effective therapeutic method.

目的:探讨卡培他滨联合常规化疗治疗晚期胃癌疗效、不良反应及对血清基质金属蛋白酶2（matrix metalloproteinase 2,MMP-2）及MMP-9的影响,为临床治疗晚期胃癌提供临床依据.方法:收集南华大学附属第二医院2011-10/2013-10收治的120例胃癌患者,随机将其分为对照组57例与观察组63例.对照组给予常规多西紫杉醇和顺铂治疗,观察组在常规治疗基础上给予卡培他滨治疗,观察并比较两组患者疗效、生存率、不良反应及对血清MMP-2和MMP-9水平的影响.结果:两组患者治疗有效率比较差异无统计学意义（P＞0.05）,但观察组6 mo与1年生存率分别为88.9%与77.8%,均显著高于对照组患者（73.7%vs 59.6%）,差异具有统计学意义（P＜0.05）.观察组患者在骨髓抑制及胃肠道反应方面显著高于对照组患者,差异具有统计学意义（P＜0.05）,两组患者其他方面不良反应差异无统计学意义.两组治疗后与治疗前相比,血清MMP-2与MMP-9水平均有所降低,差异均具有统计学意义（P＜0.05）.与对照组相比,观察组治疗后MMP-2与MMP-9均显著低于对照组治疗后血清水平,差异均具有统计学意义（P＜0.05）.结论:卡培他滨联合常规化疗治疗晚期胃癌疗效确切,安全性较好,并且可显著降低M M P-2与M M P-9的血清水平,值得临床上进一步深入研究.

目的 观察低频电刺激和吞咽训练配合康复护理干预治疗脑卒中后吞咽功能障碍患者的疗效。方法 将124例脑卒中后吞咽障碍患者分为观察组和对照组，每组62例，观察组采用低频电刺激及吞咽训练，并配合康复护理，对照组采用吞咽训练。2组在治疗前、后吞咽障碍程度采用洼田饮水试验进行评估。结果 治疗后2组患者的吞咽障碍程度评分均较治疗前明显改善，治疗后观察组明显优于对照组(P＜0.05)。结论 低频电刺激和吞咽训练治疗配合康复护理干预在改善脑卒中患者吞咽功能障碍方面较单纯吞咽训练具有更好疗效。

OBJECTIVE To investigate the preventive effect of aloe gel on doxorubicin-induced extravasation injury and the underlying mechanism.METHODS Sprague-Dawley(SD) mice were used to establish the extravasation injury model induced by doxorubicin.Seventy SD mice were randomly divided into seven groups:non-injured control group,simulated operation group,doxorubicin-induced extravasation injured group,dissolvant control group,hyp-concentration(0.1 g·L-1) aloe gel protected group,meta-concentration(1.0 g·L-1) aloe gel protected group,hypsi-concentration(10.0 g·L-1) aloe gel protected group.The areas of the extravasation injuries were measured to observe the degree of doxorubicin-induced extravasation injury.The pathological morphology was observed by optical microscopy.Expression of basic fibroblast growth factor(bFGF) and intercellular adhesion molecule(ICAM-1) in the exosmosis skin and subcutaneous tissues was detected by immunohistochemistry.RESULTS The areas of extravasation injuries in aloe gel protected groups at different concentration were smaller than that in doxorubicin-induced extravasation injured group(P0.05).The result of HE stain revealed that the degree of extravasation injuries in aloe gel protected groups at different concentration was obviously less severe than that in doxorubicin-induced extravasation injured group.The result of immunohistochemistry revealed that the expression level of bFGF was lower and ICAM-1 was higher in doxorubicin-induced extravasation injured group than that in non-injured control group,while the expression level of bFGF was gradually higher and ICAM-1 was gradually lower in aloe gel protected groups at different concentration than that in doxorubicin-induced extravasation injured group,in a dose-dependent manner.CONCLUTION Aloe gel prevents doxorubicin-induced extravasation injury.Its mechanism is related with the up-regulation of bFGF expression and down-regulation of ICAM-1 expression.