Objective To establish a new method which use nitrocellulose(NC)membrane as a cell cul-ture carrier to replace coverslips.Methods After simple pretreatment of NC membrane,cell lines were cultured on the membrane within culture medium,in parallel with the same cell culture on the coverglass which served as control.Results NC membrane was shown spreading,transparence,edge sharpness companied with purple background with macroscopic observation.Under microscopic observation,there was no significant difference for the cell morphology,cell number between NC membrane culture and coverslip culture.NC mem-branes showed transparence,sharp edge and regular morphology.Fusiform shape of LOVO cells,short rod-shape of SW480 cells and irregular polygon shape of SW1116 cell were found on the NC membrane.In addition,cells were grown in colony pattern and showed blue nucleus and red cytoblasm.Conclusions NC membrane via simple pretreatment was no toxicity to the cells,and can be used in cell culture.It not only kept the cell normal morphology and cell number,but also met the transparence for microscope observation.

OBJECTIVE:To study the microstructue of acellular dermal matrix (ADM) and the attachment and proliferation of MG63 osteoblast-like cells on the membrane.METHODS:ADM was experimental group and expanded polytetrafluoroethylene (e-PTFE) membrane was control group. Light microscope and scanning electronic microscope (SEM) were used to observe the microstructure of the two kinds of membrane. MG63 osteoblast-like cells were cultured on the two kinds of membrane and the blank group. The cell viability was checked by cell viability analyzer (CVA) and alkaline phosphatase (ALP) activity was detected by ELISA. SEM was used to observe the adhesion and proliferation of the MG63 osteoblast-like cells on the two kinds of membrane.RESULTS:The ADM was composed of the tissular side and basal lamina side. The tissue side showed scale-shaped structure and the basal lamina side demonstrated many digitations and some pores of folliculus pili. The e-PTFE showed many tiny lined cracks in elliptic structure. The viability of the MG63 osteoblast-like cells on the two kinds of membranes and ALP measurement showed that there was no significant difference between each of the two membrane groups and the blank. The adhesion and proliferation of osteoblast-like cells on the two kinks of membranes were both well.CONCLUSION:ADM has no negative effects on the growth of osteoblast-like cells. ADM is biocompatibile and its microstructure is appropriate for guided bone regeneration materials.

目的踝关节功能在偏瘫步态中具有重要作用,其功能恢复的好坏与脑病变的部位及大小有密切关系[1,2].许多偏瘫患者在康复后常遗留有踝背屈障碍及跟腱挛缩.神经肌肉电刺激疗法(neuromuscular electrical stimulation,NMES)对偏瘫患者有一定的疗效,与主动运动训练配合具有相互协同的作用,本实验通过对腓总神经电刺激进行脑功能成像的研究,进而对NMES在脑恢复机理方面的作用作一定的探讨.方法健康受试者6人,分别电刺激其腓总神经致踝关节背屈,同时利用1.5T fMRI扫描头颅,所得资料经MRICro软件转换格式,再用SPM99软件进行数据处理及统计学分析(P=0.001).结果兴奋区:对侧第一躯体感觉皮质(primary somatosensory cortex,SⅠ)、第二躯体感觉区(secondary somatosensory area,SⅡ),同侧SⅡ、第二躯体运动区(secondary motor area,MⅡ),以及双侧顶上小叶和顶下小叶;抑制区:按P=0.001标准,双侧均没有阳性结果,按P=0.05标准,则在对侧顶上小叶和顶下小叶交汇处有小片阳性区域,涉及7区.结论腓总神经电刺激致踝关节背屈时对大脑的影响是多方面的,其促进损伤后大脑运动功能的恢复可能是激活了随意运动的中枢编程和感觉性反馈环节.