OBJECTIVES:To understand the relation between nitric oxide (NO) level in seminal plasma and male fertility.METHODS:The levels of NO in seminal plasma of 174 fertile males and 217 abnormospermia patients were measured by using the reductase of nitric acid, Greiss reagent and spectrophotometry.RESULTS:1. NO was found in all 174 samples (20-49 years) of fertile males which was (27.78 +/- 5.81) mumol/L. The NO level in seminal plasma in fertile males was became higher after age 40, and there was no significant difference between 20-29-year-old [(26.25 +/- 5.52) mumol/L] and 30-39-year-old[(28.11 +/- 5.87) mumol/L]. But the group of 40-49-year-old[(30.17 +/- 6.14) mumol/L] had a higher level of NO in seminal plasma than 20-29-year-old (P < 0.05). 2. The seminal plasma samples from nine types of abnormospermia were measured, which all had a higher level of NO than fertile males. In abnormospermia, the level of NO in seminal plasma of the patients with single abnormality increased slightly, two abnormality obviously increased, and the highest level of NO in seminal plasma appeared in three abnormality.CONCLUSIONS:This results confirmed that proper level of NO in seminal plasma may regulate the spermatogenesis.

Objective To detect the effect and clinical significance of the serum level changes of nitric oxide (NO) and alpha granule membrane protein (GMP 140)in patients with coronary heart disease (CHD) Methods The serum levels of NO and GMP 140 were measured in 40 patients with CHD (22 patients with unstable angina,18 patients with stable angina) and 45 control subjects by Griess calorimetric method and enzyme linked immunosorbent assay Results ①Serum levels of NO were significantly lower in patients with CHD than those in control group ( P 0 01) ②Serum levels of NO were significantly lower in patients with unstable angina than those in stable angina,serum levels of GMP 140 were significantly higher than those in stable angina ③Negative correlation was present between the NO and GMP 140 ( P 0 01) Conclusion The result shows that serum levels of NO and GMP 140 play an important role in the happening and development of CHD;measuring the serum of NO and GMP 140 is helpful for indicating the severity of CHD and its therapeutic effect

子宫内膜异位症(endometriosis,EM)是育龄妇女的常见病,在临床中发病率呈升高趋势,其5年以内的复发率在40%以上.近年来的研究发现,EM的发病与细胞因子有着密切的关系.

子宫内膜异位症(endometriosis，EM)，是育龄妇女的常见病、多发病，由EM引起不孕的发病率高达30％～40％。尤其目前对轻症EM不孕患者，是用外科还是内科治疗来提高生育力尚缺乏统一的认识，这其中部分原因是：我们缺乏对EM不孕的病理生理机制的认识。为此本文探讨了轻症EM所致不孕患者的血清、腹腔液IL-6及EMAb水平，将有益于指导EM不孕的临床治疗。