目的 探究颈动脉狭窄程度对机械取栓后颅内侧支循环状态的影响.方法 选取邯郸市中心医院2019年1月至2020年12月80例急性缺血性脑卒中(AIS)病人作为研究对象,根据颈动脉狭窄程度分为轻度狭窄组(n=23)、中度狭窄组(n= 39)、重度狭窄及闭塞组(n=18),均行机械取栓,比较三组临床资料、机械取栓前后颅内侧支循环状态[神经介入和治疗神经放射学会/介入放射学会(ASTIN/SIR)、基底动脉CT血管造影(BATMAN)评分],分析颈动脉狭窄程度与ASTIN/SIR、BATMAN评分相关性,随访90 d,统计三组预后情况,评价颈动脉狭窄程度对颅内侧支循环状态、预后的影响,并采用KM曲线进行生存分析.结果 机械取栓后轻度狭窄组、中度狭窄组、重度狭窄及闭塞组ASTIN/SIR评分分别为(3.42±0.28)分、(3.05±0.22)分、(2.63±0.17)分和BATMAN评分分别为(8.24±0.71)分、(7.13±0.63)分、(5.29±0.42)分均高于机械取栓前(1.31±0.36)分、(0.87±0.21)分、(0.52±0.17)分和(4.78±1.12)分、(3.36±0.87)分、(2.09±0.54)分(P＜0.05);且重度狭窄及闭塞组机械取栓前、机械取栓后ASTIN/SIR、BATMAN评分均低于中度狭窄组、轻度狭窄组,中度狭窄组均低于轻度狭窄组(P＜0.05);Spearman相关性分析,颈动脉狭窄程度与机械取栓前、机械取栓后ASTIN/SIR、BATMAN评分呈负相关(P＜0.05);单因素分析,颈动脉狭窄程度与颅内侧支循环状态、预后的显著相关(P＜0.05);随访90 d,重度狭窄及闭塞组预后不良率61.11％高于中度狭窄组35.90％、轻度狭窄组13.04％,中度狭窄组预后不良率高于轻度狭窄组(P＜0.05);KM曲线分析,重度狭窄及闭塞组90 d生存率55.56％(10/18)低于中度狭窄组89.74％(35/39)、轻度狭窄组95.65％(22/23)(P＜0.05).结论 颈动脉狭窄程度对AIS病人机械取栓后颅内侧支循环状态与预后均有重要影响,术前准确判断颈动脉狭窄程度并采取针对性治疗措施有利于改善颅内侧支循环状态,强化机械取栓效果,降低预后不良及死亡风险.

Objective To observe the expression changes of microRNA181c (miR-181c) in glioblastoma (GBM) and its effects on invasion and migration of tumor cells.Methods real-time PCR was used to detect the miR-181c expression in 41 GBM tissues (GBM group) and 15 normal brain tissues (control group).GBM cell line T98G was divided into four groups:groups A, B, C and D.In the groups A, B and C, the T98G was transfected with miR-181c with overexpression, control miRNA and miR-181c, respectively, but the group D without any treatment.After transfection 48 h, the invasion and migration in each group was investigated.Results Compared with the control group, the miR-181c expression was down-regulated in the GBM group, P<0.05.After transfection 48 h, the invasive cells in the groups B, C and D were more than those of the group A, P<0.05.Moreover, cell relative migration distance in the groups B, C and D was also longer than that of the group A, P<0.05.Conclusion The miR-181c is lowly expressed in GBM tissues, and the low miR-181c expression is closely related with GBM cell invasion and migration.

目的 探讨微小RNA-490-3p(miR-490-3p)对胶质瘤细胞增殖和凋亡的影响,观察miR-490-3p对胶质瘤生物学行为的影响.方法 通过荧光实时定量PCR(qRT-PCR)检测16例胶质瘤样本、胶质瘤旁组织及4种胶质瘤细胞系中miR-490-3p的表达;利用人工合成miR-490-3p mimic瞬时转染脑胶质瘤细胞株,qRT-PCR检测细胞中miR-490-3p的表达水平;采用MTT比色法检测胶质瘤细胞增殖情况;流式细胞术检测胶质瘤细胞凋亡.结果 胶质瘤样本及胶质瘤细胞系中miR-490-3p的表达量较瘤旁与正常胶质细胞系显著降低,miR-490-3p mimic能够显著上调胶质瘤细胞株中miR-490-3p的表达水平,并显著抑制胶质瘤细胞株U251、U87细胞的增殖能力显著增加细胞凋亡数量;结论 miR-490-3p在胶质瘤样本及胶质瘤细胞系中呈现低表达,过表达miR-490-3p有效抑制了胶质瘤细胞株的增殖,增加凋亡,提示miR-490-3p可能成为胶质瘤治疗的新靶点.

Objective To investigate the mechanism of miR -181c inhibiting the invasion of glio‐blastoma cell line T98G .Methods Using RT -PCR and Western Blot to detect the expression of miR -181c and TGF-βrelated genes .The effect of miR -181c and TGF -βon the invasion of glioma cells was measured by Transwell .Using molecular cloning technology to design and clone the target gene 3'-UTR and its mutation region .Using luciferase assay to analyse the combination of miR -181c and target genes .Results mRNA and protein expression of miR -181c and TGF -βpathway associated genes TG‐FBR1 ,TGFBR2 and TGFBRAP1 decreased (P< 0 .05) .miR-181c could weaken the invasion of T98G cells by influencing TGF -βsignal and the 3'-UTR of TGFBR1 ,TGFBR2 and TGFBRAP1 were direct targets of miR-181c .Conclusions miR -181c can inhibit the invasive ability of glioma cell line T 98G by regulating the TGF -βsignaling pathway ,and it can be an important molecule for glioblastoma treatment .

Objective Todetecttheexpressionlevelsoftumornecrosisfactor-α(TNF-α)and interleukin-6(IL-6)inintracranialaneurysms.Methods Sixteenconsecutivepatients(aneurysm group)with intracranial aneurysm confirmed by digital subtraction angiography (DSA)and clipped by microneurosurgery were enrolled retrospectively. A total of 19 trauma patients without vascular disease confirmed by CT and magnetic resonance imaging (MRI)in the same period were used as a control group. Hematoxylin-eosin (HE)staining and immunohistochemical staining were used to detect the aneurysm wall tissue and the colored portions of TNF-α and IL-6 in normal vessel wall,the mean value of optical density after its expression was analyzed,and the intensity of staining was compared. Results (1)Each layer of artery walls of the control group had no obvious TNF-α and IL-6 expression. The inner,media and out membranes of the aneurysm wall tissue of the aneurysm group had positive expression of TNF-αand IL-6. (2)The mean optical densities of TNF-α and IL-6 in patients of the aneurysm group were 0. 182 ± 0. 069 and 0. 148 ± 0.062 respectively,and they were higher than 0. 144 ± 0. 031 and 0. 105 ± 0. 020 of the control group. The differences were statistically significant (all P<0. 05). (3)The mean optical densities of TNF-α expression of each layer of the inner,media and out membranes in the aneurysm walls were 0. 224 ± 0. 071,0. 134 ± 0. 040,and 0. 106 ± 0. 065,respectively. There were significant differences (P<0.01). (4)The mean optical density expressed by IL-6 in the out membrane of the aneurysm walls was lower than the media and inner membranes (0. 096 ± 0. 018 vs. 0. 145 ± 0. 050,and 0. 148 ± 0. 070). There were significant differences (P<0. 05). (5)The results of Spearman correlation analysis showed that the mean optical density of TNF-αof the aneurysm group was positively correlated with that of IL-6 (r=0. 452, P<0.05).Conclusion TheexpressionlevelsofTNF-αandIL-6intheaneurysmwalltissueare higher,and they may be involved in intracranial aneurysm formation and rupture.