目的 研究血清1,3-β-D葡聚糖检测(G试验)、半乳甘露聚糖检测(GM试验)对非粒细胞缺乏侵袭性肺曲霉病(IPA)的诊断价值.方法 选取2019年6月至2020年7月该院收治的非粒细胞缺乏IPA患者53例(IPA组)作为研究对象.另选取同期该院收治的非粒细胞缺乏细菌性感染患者51例作为非IPA组.对所有患者进行血清G试验与GM试验检测,对试验结果进行比较,并绘制受试者工作特征曲线(ROC曲线)分析血清G试验、GM试验对非粒细胞缺乏IPA的诊断效能.结果 IPA组GM试验结果比非IPA组高,差异有统计学意义(P<0.05);IPA组G试验结果比非IPA组高,差异有统计学意义(P<0.05).血清GM试验和G试验联合诊断非粒细胞缺乏IPA的ROC曲线下面积为0.663.随着GM试验、G试验临界值增高,GM试验、G试验诊断非粒细胞缺乏IPA的灵敏度降低,特异度升高.结论 血清GM试验和G试验联合检测对非粒细胞缺乏IPA患者早期诊断具有一定价值,动态监测血清GM试验结果对其疗效有着重要的临床意义.

Objective To Inverstigate the distribution characteristics and drug-resistance of Acinetobacter baumannii isolated from a hospital, to provide evidence for rational use of antibacterials in clinical practice. Methods A retrospective analysis was conducted. data of Acinetobacter baumannii isolated from hospitalized patients in a hospital in recent 6 years were collected, and clinical distribution and drug-resistance were analyzed by WHONET 5.6 and SPSS16.0 software. Results In the 714 Acinetobacter Baumanii isolates, 363 strains(50.84％) were from intensive care unit (ICU), followed by the department of respiratory medicine(9.66％).558 strains(78.2％) were isolated from sputum. Among the clinically used antibiotics, the resistance rate to most of antibiotics were >50％, including carbapenems, aminoglycosides and quinolones, especially in the ICU. The drug-resistance rate to 12 antibiotics and the detection rate of carbapenem-resistant Acinetobacter baumannii was significantly higher than that of non-ICU(P< 0.005). Conclusion The clinical distribution of Acinetobacter baumannii is widely distributed, and the trend of multidrug resistance and multidrug resistance is obvious,The difference of resistance rate between different departments was obvious. It's necessary to strengthen the monitoring of drug resistance and use antibiotics reasonably.

Objective To explore the role of mouse interleukin-6 receptor fusion protein (mIL-6RFP)for the pro-tection of liver cells in the model of BALB /c mice infected with Schistosoma japonicum.Methods Nickel ion col-umn was used to obtain purified recombinant mIL-6RFP protein.In vitro,HepG2 cells were used to verify the bio-logical activity of mIL-6RFP.BALB /c mice were administrated by tail vein injection with 100 μg of mIL-6RFP or equal volume solvent,at 24,26,28,30,32,34,36,38,and 40 days after infection.Mice were sacrificed 48 h after the last injection.The granuloma size was measured in the mouse liver by HE staining.Interleukin-1β(IL-1β),interleukin-13 (IL-13 ),tumor necrosis factor-α (TNF-α) and chemokine (C-X-C motif) ligand 1 (CXCL1)mRNA levels in mouse liver tissue were detected by RT-PCR.Alanine aminotransferase (ALT)and as-partate transaminase (AST)were measured by the continuous monitoring method.Results HepG2 cells experi-ments showed that mIL-6RFP could reduce the effect of IL-6 on HepG2 cells,fibrinogen (FGG)and haptoglobin (HP)was significantly reduced (P <0.05).In the BALB /c mice infected with Schistosoma japonicum,when IL-6 was blocked the levels of ALT and AST were decreased significantly (P <0.05)compared with that of the solvent group,while there was no significant difference on granuloma formation.Conclusion In the BALB /c mice infec-ted with Schistosoma japonicum,mIL-6RFP can significantly improve the liver function but no obvious effect on granuloma formation.

Objective To investigate the anti-schistosomiasis effect of Tsp2/Sj29 ku protein from Schistosoma japonicum. Methods The recombinant protein pet32a/SjTsp2/Sj29 ku induced and expressed by IPTG was puri-fied. Twenty nine female 6-week old mice were randomly divided into 3 groups ( Protein vaccine Group, Thioredox-in Group and Control Group). In Protein vaccine Group (n=10), each mouse was immunized subcutaneously with 50 μg SjTsp2/Sj29 ku plus equal volume Freund's complete adjuvant in 0 day. 12 and 24 days later, each mouse was re-immunized subcutaneously with 50 μg SjTsp2/Sj29 ku plus equal volume Freund's incomplete adjuvant. In Thioredoxin Group ( n=10 ) and Control Group ( n=9 ) , each mouse was immunized with thioredoxin and saline respectively. Ten days after last immunization, each mouse was challenged with 30±2 cercaria of Schistosoma ja-ponicum. On the 45th day post-infection, the mice were exeuted. The worm and egg reduction rates were calculat-ed. The sera was collected from orbital blood. The levels of IgG1, IgG2a, IgG2b, IgG3 and IgM antibodies in mouse sera were detected by enzyme-linked immunosorbentassay ( ELISA) . The level of IFN-γ and IL-4 was also detected by ELISA. Results Pet32a/SjTsp2/Sj29 ku was obtained as expected. The worm reduction rates and egg reduction rates in Protein vaccine Group were 23. 95% (P<0.05)and 55. 57% (P<0.05) respectively, com-pared with the Control Group. There was no statistical significance ( P >0.05 ) between Thioredoxin Group and Control Group in worm reduction rates and egg reduction rates. In Protein vaccine Group, IgG1, IgG2a and IgG2b were remarkably higher than that of Control Group, while IgG3 and IgM did not remarkably change. In comparison with the Control Group, the levels of IFN-γand IL-4 of the mice in Protein vaccine Group were obviously augmen-ted (P<0.01). Conclusion The mice, which have been immunized with Tsp2/Sj29 ku show a partial immune protection;the protein vaccine of SjTsp2/Sj29 ku could induce the mixed immune responses of Th1 and Th2 .

目的 构建以及表达鼠白介素6 (IL-6)受体融合蛋白.方法 采用基因重组技术将鼠IL-6受体融合基因克隆到杆状病毒质粒Bacmid上,重组杆状病毒质粒在脂质体的介导下转染昆虫细胞Sf9,转染后收集重组杆状病毒,采用空斑实验检测第3代重组杆状病毒的滴度,并通过Western blot来确定目的蛋白的表达.结果 重组杆状病毒质粒在DH10Bac大肠杆菌中包装成功,并转染Sf9细胞后得到重组杆状病毒,第3代重组杆状病毒的滴度为5×107 pfu/ml,Western blot表明目的蛋白在Sf9细胞中表达.结论 成功表达了鼠IL-6受体融合蛋白,为进一步研究IL-6在炎症性疾病中的作用奠定了实验基础.