Good diluent is one of the key factors to improve the quality of porcine sperm in artificial insemination of swine.In order to investigate the effects of carboxymethylcellulose sodium (CMC) on the quality of porcine semen at room temperature,CMC was added at concentrations of 8,16 and 24 μmol/L CMC to the extender of Zorlesco(-BSA).Boar sperm viability,motility,membrane integrity and acrosomal completion were detected by Computer-assisted semmen analyzer (CASA),Hypo-osmotic swelling test (HOST),and Coomassieblue staining,respectively.The results showed that Zorlesco as basic dilution appended with 8～24 μmol/L CMC could increase the sperm viability compared to the control group within the first 3 d in semen preservation,though without obvious difference (P＞0.05).The sperm viability in treatment group with 16 μmol/L CMC was significantly improved compared with the control group within 4～7 d.The sperm plasma membrane integrality in treatment groups with 8～24 μmol/L had no obvious difference within the first 4 d compared to the control group,but the plasma membrane integrality in treatment group with 16 μmol/L CMC was significantly improved compared with the control group within 5～7 d.The results showed that Zorlesco with 8～24 μmol/L CMC can improve boar sperm viability,motility,plasma membrane integrality and acrosome integrality,and the Zorlesco with 16μmol/L CMC was the best.In conclusion,CMC can be used as a suspending agent in semen diluent to slow down the sedimentation rate of sperm and improve the quality of porcine semen stored at room temperature.

为了验证一种新型功能性预混料对种公猪的作用效果,将20头种公猪随机分为试验组和对照组.对照组饲喂基础饲粮,试验组在基础饲粮的基础上每头每天按4％添加功能性预混料,连用10周.结果表明:饲喂功能性预混料后,第6周末试验组种猪的采精量、精子密度和精子活率均显著高于对照组(p＜0.05),呈逐渐升高的趋势,而精子畸形率、顶体不完整率均显著低于对照组(p＜0.05),也有逐渐下降的趋势.说明本试验使用的功能性预混料能够明显改善种公猪的精液品质.

【Objective】Differ ent expression profiles of polypeptides in follicle fluid from superovulated cattle and calves were studied in order to provide references for further research on follicle fluid of calves and in vitro oocyte culture system of calves.【Method】The matrix-assisted laser desorption ionization mass spectrometry(MALDI-TOF MS)was used to analyze the peptide fragments in follicular fluid of superovulated cattle and calves.Based on signal peak information of polypeptide,protein search was conducted in MASCOT database.【Result】The models of polypeptide expression profiles of the two groups were successfully established,respectively.There were 30-40 peak signals in cattle group,and 60-80 peak signals in calves.By comparing the peak lists from the two different samples,it was found that 2 specific peaks of 1218.58 and 1509.77 m/z appeared in cattle group,similarly,and 1 specific peak of 953.54 m/z in calf group.At last,MASCOT library was used to search the information of polypeptides,and then some proteins related to these polypeptides information were gained.The results showed that 18 kinds of proteins existed in cattle group,and 11 kinds of proteins in calves.【Conclusion】The results showed that the polypeptide information peak quantity of calf follicular fluid protein was higher than that of adult cattle,while the total number of proteins in follicle fluid from the calves was less than that of the adult cattle group,which could be one of reasons why the developmental competence of oocytes from calves was poorer than that of adult cattle.

In order to estabilish and optimize a two dimensional gel electrophoresis(2-DE)technique for proteome analysis of calf follicular fluid,effects of two different IPG strips and 4 kinds of loading volumes(150 μg,250 μg,350 μg,450 μg) on the quanty of the 2-D maps were studied.The results showed that one perfect and clearly 2-DE map was obtained when pH 4~7 IPG strip with 24 cm and loading volume of 350 μg were used.About 319 spots in the 2-DE map were detected by the Image Master 2-D platinum software.Our established 2-DE technique of calf follicular fluids can be effectively applied in different peoteins between calves and adult cattles.

以ZORLESCO(-BSA)稀释液为公猪精液常温保存基础稀释液,研究聚乙烯吡咯烷酮(PVP)、聚乙烯醇(PVA)含量对17℃公猪精液保存的影响。结果发现,在ZORLESCO(-BSA)稀释中液添加0.4%PVP和0.4%PVA保存的效果较好,其中以0.4%PVP保存效果最佳。

本研究的目的是比较美系长白、大白和杜洛克种公猪睾丸体积、精清中睾酮含量与精液品质的关系。研究采集了44头杜洛克、31头长白和22头大白种公猪的精液并测定其精液品质,测量其睾丸的长度、宽度和厚度。结果表明,种公猪睾丸体积从大到小依次为大白、长白、杜洛克;长白在精子密度、活力和畸形率方面是较好的,杜洛克精液品质是3个品种中较差的;睾酮含量与精液品质呈弱相关,睾酮含量与睾丸体积不相关,精液品质与睾丸体积不相关。