The freezing tolerance of many plants is increased by exposure to low temperature or application of ABA, CCC. Activities of amylase and peroxidase correlate with cold resistance in many research reports. Amylase and peroxidase activities of Jojoba shoots in vitro were tested after different cold acclimation time or different frozen temperature treatments. Significant differences existed among clones in the increase of activities although there were same trends in clones. After 7 days acclimation both activities reached their highest level in the expriments and sim- ilar trends were found when the Jojoba shoots were treated by frozen temperature as -- 8℃, which results were con- firmed by 3 repeats. When the frozen temperature decreased lower than -- 8℃, both enzymes became less active. ABA or CCC at different concentrations was applied to the propagation media of Jojoba clones. 8 weeks later, the activities of amylase and peroxidase were measured and analyzed, and it demonstrated that ABA or CCC application at Jojoba shoots affected the metabolism activities such as those of amylase and peroxidase, and the both enzymes shown the highest activity when ABA at 1mg/L or CCC at 2mg/L was applied at medium.

利用电子辐射处理好好芭试管苗,从改变基因型入手提高其抗寒力,以获得稳定遗传的好好芭株系.研究表明150GY的辐射量是诱发好好芭发生变异的最佳剂量,结合生化标记,抗寒相关的酶类--淀粉酶和过氧化物酶活性分析,人工模拟低温实验的克隆检测,筛选出了具有较高抗寒能力的好好芭株系,这些株系通过无性系繁殖可用于抗寒育种.