Objective Seasonal influenza vaccine is effective to reduce the risk of hospitalization and death in vulnerable older persons, but the coverage of influenza vaccine remains low in these population in China. This study thus aimed to investigate how adults' vaccine literacy affects their intention to recommend influenza vaccination to older family members in China. Methods A cross-sectional survey was performed in China during October 7 to 16, 2023 through a web-based questionnaire. The participants' influenza vaccine literacy (IVL) was quantitatively assessed using an adapted scale, and bivariate analyses were undertaken to evaluate variable associations among the participants. Multivariable logistic regression and mediation effect model were used to analyze the target factors. Results 3027 valid respondents were included in our survey, 89.6 % of them were willing to recommend their older family members for influenza vaccine. Participants with higher IVL scores were more likely to recommend influenza vaccination (OR = 1.553, P < 0.01). In addition, participants who were living with their older family members, received annual influenza vaccinations, or whose older family members had infected influenza within the past six months, were also more likely to do so. Of note, despite of a higher level of vaccine literacy, healthcare works were more reluctant to recommend vaccination for older family members (OR = 0.496, P < 0.05). Conclusion High level of vaccine literacy is positively correlated to adults' intention to recommend influenza vaccination for older family members. We should take educational interventions of vaccine literacy among adults, and thus promoting influenza vaccination coverage among the older population.

BackgroundBlood inflammatory status is closely associated with tuberculosis (TB) progression. Emerging inflammatory indices from different leukocyte subtypes have become a prognostic hotspot for various diseases, yet their application in TB prognosis remains limited. This study aims to assess the impact of inflammatory status on TB patients’ prognosis and its potential as a prognostic indicator to optimize prognostic assessment and therapeutic strategies.MethodsThis study included 4027 TB patients admitted to a tuberculosis-designated hospital in Shenzhen from January 2017 to December 2022. Patients were classified into three inflammatory statuses (Q1-Q3) based on each index’s level. We conducted Cox regression and restricted cubic splines (RCS) analyses to evaluate the association between inflammatory status and unfavorable outcome, subgroup analyses to understand heterogeneous associations among subpopulations, and receiver operating characteristic (ROC) analyses to evaluate the prognostic performance of inflammatory status on TB treatment outcomes.ResultsDuring 48991.79 person-months of follow-up involving 4027 patients, 225 unfavorable outcomes occurred. Multivariable Cox regression indicated that the Q3 levels of CAR, CLR, dNLR, NLR, SII, and SIRI increased the risk of unfavorable outcome by 45%-99% (HR: 1.45-1.99, all P<0.050), whereas ENR reduced the risk by 29% (HR: 0.71, P=0.040) compared to Q1. RCS curves revealed linear associations with unfavorable outcome that were positive for CAR, CLR, dNLR, SII, and SIRI, negative for ENR (all P for nonlinear>0.050), and nonlinear for MLR, NLR, and PNI (all P for nonlinear<0.050). Subgroup analyses identified heterogeneous associations across age, sex, BMI, comorbidities, and drug resistance (all P for interaction<0.050), with attenuated risk effects of CAR, CLR, dNLR, and SII in patients aged 30-60 years, male, BMI≥24.0 kg/m², smokers, retreatment cases, and those with tumor. ROC analysis demonstrated stable predictive performances of inflammatory status (AUC: 0.785–0.804 at 6-month, 0.781–0.793 at 9-month, and 0.762–0.773 at 12-month), and the combination of the inflammatory status significantly optimized the prognostic performance of the basic model (9-month AUC: 0.811 vs 0.780, P=0.024; 12-month AUC: 0.794 vs 0.758, P=0.013).ConclusionPretreatment blood inflammatory status effectively predicts the treatment outcome of TB patients. Our findings hold significant clinical value for TB patient management and warrant prospective evaluation in future studies.

The effective mass envelope function and diagonalization methods are used to obtain the sub-band energy eigenvalues and eigenfunctions of the structure. Then, the nonlinear refractive index changes of the structure are approximated by the compact density matrix. Hereafter, the structural parameters of the quantum well and the external electromagnetic field are adjusted to maximize the refractive index change response, whereas the dipole matrix elements are regulated according to the degree of energy separation between the wave functions, aiming to improve the optical performance and clarify the value of parameter adjustment. Our findings can be of interest in parameter design and experimental applications of optoelectronic devices for the selection of the more appropriate nonlinear optical configuration.

Disrupted in Schizophrenia 1 (DISC1) is essential for neuronal development and has been implicated in various psychiatric disorders. Our transcriptomic and proteomic analyses identified Zika virus (ZIKV) infection enhanced DISC1 expression, however, its functional role in ZIKV infection and caused congenital Zika syndrome (CZS) and ZIKV-induced long-term neurodevelopmental defects remain unexplored. In this study, we demonstrate that DISC1 attenuates ZIKV infection in human placental and neuroglia cells, as well as in murine macrophages and primary cortical cells. DISC1 also decreases ZIKV dissemination from peripheral tissues to key organs of mice, including the uterus, testis, and brain, thereby reducing fetal abortion rates and intrauterine growth restriction. Notably, DISC1 is associated with brain damage and long-term ZIKV effects, including memory loss, reduced anxiety and depression, declines in sociability and social novelty. Mechanistically, DISC1 activates autophagy by enhancing AMPKα phosphorylation and reducing mTOR phosphorylation, protecting against ZIKV infection. Additionally, DISC1 interacts with LC3 to further activate autophagy, partially contributing to reduce ZIKV infection. In conclusion, DISC1 plays a critical factor in controlling ZIKV infection and mitigating CZS and ZIKV-induced neurocognitive decline.

Nanodiamond/Mo reinforced in-situ synthesized NiAl-based composite coatings were prepared by laser cladding. The microstructure, microhardness, and tribological properties of these composite coatings were studied. The results show that the coating is mainly composed of NiAl and Ni3Al phases. ND and Mo show a synergistic effect, which not only reduces the porosity of the coating, refines the grain size, and also improves the microhardness. In addition, in the temperature range of 25-800 degrees C, they reduce the friction coefficient and wear rate of the coating. These findings provide a valuable reference for optimizing the performance of NiAl-based composite coatings.

In this study, NiCrBSi-based, NiCrBSi+CrsCI, NiCrBSi+WC+CrsCI, and NiCrBSi+WC fused coatings (designated as A, B, C, and D, respectively) were prepared using laser cladding combined with the pre-placed powder method. Comparative analysis of the microstructure and surface properties revealed that the primary phase of all four coatings was a gamma-(Fe, Ni) solid solution, while the addition of CrsCI and WC promoted the formation of carbides such as MIsC6, WIC, and WC. Specifically, in fused cladding layer A, the root region predominantly exhibited columnar crystals with sporadic cellular dendrites, arranged in a "fishbone" pattern and growing perpendicular to the interface toward the coating surface. For cladding B, dense cellular grain bands were observed near the root, while the upper region transitioned into a mixture of columnar and cellular crystals. The fused cladding layer C displayed a layered microstructure, consisting of honeycomb and columnar crystals at the coating-substrate interface, dendritic crystals in the inner region, and spherical WC particles along with polygonal CrsCIparticles uniformly dispersed within the coating matrix. The fusion-coated layer D exhibited a disordered arrangement of coarse cellular dendrites and irregular dendritic structures in the root region. The dual-hardened phase-reinforced NiCrBSi fused cladding C demonstrated the highest average microhardness (1080.5 HV0.I) and the lowest frictional wear mass loss, attributed to the synergistic pinning effect of WC and CrsCI particles, which inhibited plastic deformation of the surface layer. In electrochemical tests, this coating exhibited superior passivation behavior, displaying a more positive corrosion potential and lower current density compared to the other coatings. The simultaneous enhancement in wear and corrosion resistance is ascribed to the uniform distribution of WC/CrsCI particles, which effectively impeded crack propagation and the penetration of corrosive media.

BackgroundsThe diagnostic delay of tuberculosis (TB) contributes to further transmission and impedes the implementation of the End TB Strategy. Therefore, we aimed to describe the characteristics of patient delay, health system delay, and total delay among TB patients in Shanghai, identify areas at high risk for delay, and explore the potential factors of long delay at individual and spatial levels.MethodThe study included TB patients among migrants and residents in Shanghai between January 2010 and December 2018. Patient and health system delays exceeding 14 days and total delays exceeding 28 days were defined as long delays. Time trends of long delays were evaluated by Joinpoint regression. Multivariable logistic regression analysis was employed to analyze influencing factors of long delays. Spatial analysis of delays was conducted using ArcGIS, and the hierarchical Bayesian spatial model was utilized to explore associated spatial factors.ResultsOverall, 61,050 TB patients were notified during the study period. Median patient, health system, and total delays were 12 days (IQR: 3–26), 9 days (IQR: 4–18), and 27 days (IQR: 15–43), respectively. Migrants, females, older adults, symptomatic visits to TB-designated facilities, and pathogen-positive were associated with longer patient delays, while pathogen-negative, active case findings and symptomatic visits to non-TB-designated facilities were associated with long health system delays (LHD). Spatial analysis revealed Chongming Island was a hotspot for patient delay, while western areas of Shanghai, with a high proportion of internal migrants and industrial parks, were at high risk for LHD. The application of rapid molecular diagnostic methods was associated with reduced health system delays.ConclusionDespite a relatively shorter diagnostic delay of TB than in the other regions in China, there was vital social-demographic and spatial heterogeneity in the occurrence of long delays in Shanghai. While the active case finding and rapid molecular diagnosis reduced the delay, novel targeted interventions are still required to address the challenges of TB diagnosis among both migrants and residents in this urban setting.

SmCo permanent magnets are used in some of the strongest permanent magnets, particularly for applications between about 200 and 550 degrees C. However, their brittleness limits their practical use. Improving their mechanical properties is therefore of great practical significance. Cryogenic treatment is firstly applied in SmCo permanent magnets to enhance the mechanical properties. The flexural strength has been increased by 14.3% along the weakest orientation, and the impact toughness has been increased by 7.0%. After cryogenic treatment, the intrinsic coercivity is slightly increased, and the other magnetic properties are almost unchanged, which is different from other methods to improve mechanical properties at the expense of magnetic properties. The enhancement of flexural strength and impact toughness primarily stems from a decrease in residual stress. This study provides an effective and convenient approach to enhance the mechanical properties in Sm 2 Co 17 permanent magnets without sacrifice of magnetic properties.

Cell membrane surface tension has emerged as a pivotal biophysical factor governing cell behavior and fate. This review systematically delineates recent advances in techniques for cell membrane surface tension quantification, mechanosensing mechanisms, and regulatory roles of cell membrane surface tension in modulating major cellular processes. Micropipette aspiration, tether pulling, and newly developed fluorescent probes enable the measurement of cell membrane surface tension with spatiotemporal precision. Cells perceive cell membrane surface tension via conduits including mechanosensitive ion channels, curvature-sensing proteins (e.g. BAR domain proteins), and cortex-membrane attachment proteins (e.g. ERM proteins). Through membrane receptors like integrins, cells convert mechanical cues into biochemical signals. This conversion triggers cytoskeletal remodeling and extracellular matrix interactions in response to environmental changes. Elevated cell membrane surface tension suppresses cell spreading, migration, and endocytosis while facilitating exocytosis. Moreover, reduced cell membrane surface tension promotes embryonic stem cell differentiation and cancer cell invasion, underscoring cell membrane surface tension as a regulator of cell plasticity. Outstanding questions remain regarding cell membrane surface tension regulatory mechanisms and roles in tissue development/disease in vivo. Emerging tools to manipulate cell membrane surface tension with high spatiotemporal control in combination with omics approaches will facilitate the elucidation of cell membrane surface tension-mediated effects on signaling networks across various cell types/states. This will accelerate the development of cell membrane surface tension-based biomarkers and therapeutics for regenerative medicine and cancer. Overall, this review provides critical insights into cell membrane surface tension as a potent orchestrator of cell function, with broader impacts across mechanobiology.

The annual co-circulation of two influenza A subtypes, H1N1 and H3N2, viruses in humans poses significant public health threats worldwide. However, the continuous antigenic drift and shift of influenza viruses limited the effectiveness of current seasonal influenza vaccines, necessitating the development of new vaccines against both seasonal and pandemic viruses. One potential solution to this challenge is to improve inactivated vaccines by including multiple T-cell epitopes. In this study, we designed stabilized trimeric recombinant mosaic HA proteins named HAm, which contain the most potential HA T-cell epitopes of seasonal influenza A virus. We further evaluated the antigenicity, hemagglutinin activity, and structural integrity of HAm and compared its immunogenicity and efficacy to a commercial quadrivalent inactivated influenza vaccine (QIV) in mice. Our results demonstrated that the HAm vaccine was able to induce broadly cross-reactive antibodies and T-cell responses against homologous, heterologous, and heterosubtypic influenza-naive mice. Additionally, the HAm antigens outperformed QIV vaccine antigens by eliciting protective antibodies against panels of antigenically drifted influenza vaccine strains from 2009 to 2024 and protecting against ancestral viruses’ lethal challenge. These results suggest that the HAm vaccine is a promising potential candidate for future universal seasonal and pandemic influenza vaccine development.

Tumor necrosis factor receptor-associated factor 6 (TRAF6) is crucial in flavivirus infections, modulating the host immune response through interactions with viral proteins. Despite its importance, the relationship between TRAF6 and Zika virus (ZIKV) remains poorly understood. Our prior proteomics analysis revealed reduced TRAF6 protein levels in ZIKV-infected human trophoblast cells compared to non-infected controls. Subsequent studies in cell models and murine tissues confirmed a significant reduction in both TRAF6 mRNA and protein levels post-ZIKV infection. Further investigations unveiled that ZIKV induces P62-mediated degradation of TRAF6, with NS1 identified as the primary contributor. Co-localization and interaction studies demonstrated that NS1 promotes the association of P62, a key autophagy mediator, with TRAF6. Notably, our findings revealed TRAF6 enhances ZIKV infection, NS1 ubiquitination, NS1 expression, and the production of inflammatory cytokines and chemokines. These insights highlight the intricate TRAF6-ZIKV relationship, offering potential for drug targeting NS1-TRAF6 interactions to manage ZIKV infections effectively.

China has been continuously improving its monitoring methods and strategies to address key infectious diseases (KIDs). After the severe acute respiratory syndrome epidemic in 2003, China established a comprehensive reporting system for infectious diseases (IDs) and public health emergencies. The relatively lagging warning thresholds, limited warning information, and outdated warning technology are insufficient to meet the needs of comprehensive monitoring for modern KIDs. Strengthening early monitoring and warning capabilities to enhance the public health system has become a top priority, with increasing demand for early warning thresholds, information, and techniques, thanks to constant innovation and development in molecular biology, bioinformatics, artificial intelligence, and other identification and analysis technologies. A panel of 31 experts has recommended a fourth-generation comprehensive surveillance system targeting KIDs (41 notifiable diseases and emerging IDs). The aim of this surveillance system is to systematically monitor the epidemiology and causal pathogens of KIDs in hosts such as humans, animals, and vectors, along with associated environmental pathogens. By integrating factors influencing epidemic spread and risk assessment, the surveillance system can serve to detect, predict, and provide early warnings for the occurrence, development, variation, and spread of known or novel KIDs. Moreover, we recommend comprehensive ID monitoring based on the fourth-generation surveillance system, along with a data-integrated monitoring and early warning platform and a consortium pathogen detection technology system. This series of considerations is based on systematic and comprehensive monitoring across multiple sectors, dimensions, factors, and pathogens that is supported by data integration and connectivity.This expert consensus will provides an opportunity for collaboration in various fields and relies on interdisciplinary application to enhance comprehensive monitoring, prediction, and early warning capabilities for the next generation of ID surveillance. This expert consensus will serve as a reference for ID prevention and control as well as other related activities.

Fluoroquinolones (FQ) are essential for the treatment of multidrug-resistant tuberculosis (MDR-TB). The FQ resistance (FQ-R) rate in MDR-TB in China and its risk factors remain poorly understood. We conducted a retrospective, population-based genomic epidemiology study of MDR-TB patients in Shanghai, China, from 2009 to 2018. A genomic cluster was defined as strains with genetic distances <= 12 single nucleotide polymorphisms. The transmitted FQ-R was defined as the same FQ resistance-conferring mutations shared by >= 2 strains in a genomic cluster. We used multivariable logistic regression analysis to identify the risk factors for drug resistance. Among the total 850 MDR-TB patients included in the study, 72.8% (619/850) were male, the median age was 39 (interquartile range 28, 55) years, 52.7% (448/850) were migrants, and 34.5% (293/850) were previously treated patients. Most of the MDR-TB strains belong to the Beijing lineage (91.7%, 779/850). Overall, the genotypic resistance rate of FQ was 34.7% (295/850), and 47.1% (139/295) FQ-R patients were in genomic clusters, of which 98 (33.2%, 98/295) were presumed as transmitted FQ-R. Patients with treatment-naive (aOR = 1.84; 95% CI: 1.09, 3.16), diagnosed in a district-level hospital (aOR = 2.69; 95% CI: 1.56, 4.75), and streptomycin resistance (aOR = 3.69; 95% CI: 1.65, 9.42) were significantly associated with the transmission of FQ-R. In summary, the prevalence of FQ-R among MDR-TB patients was high in Shanghai, and at least one-third were transmitted. Enforced interventions including surveillance of FQ drug susceptibility testing and screening among MDR-TB before initiation of treatment were urgently needed.

Background: The persistently high mortality and morbidity rates of hepatocellular carcinoma (HCC) remain a global concern. Notably, the disruptions in mitochondrial cholesterol metabolism (MCM) play a pivotal role in the progression and development of HCC, underscoring the significance of this metabolic pathway in the disease's etiology. The purpose of this research was to investigate genes associated with MCM and develop a model for predicting the prognostic features of patients with HCC. Methods: MCM-related genes (MCMGs) were identified through The Cancer Genome Atlas (TCGA), The Molecular Signatures Database (MsigDB), and the Mitocarta3.0 databases. Differential gene expression analysis and least absolute shrinkage and selection operator (LASSO) Cox regression analysis were performed using R software to construct a MCM-related model. This model underwent further analysis for somatic mutations, single sample gene set enrichment analysis (ssGSEA), stromal and immune cell estimation, immune checkpoint evaluation, and drug susceptibility prediction to assess the tumor microenvironment (TME) and therapeutic responses. The mRNA expression levels of the genes associated with the model were quantified using real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Results: The model, which included six MCMGs (ACADL, ACLY, TXNRD1, DTYMK, ACAT1, and FLAD1), divided all patients (age <= 65 vs. >65 years, P<0.001; male vs. female, ns) into a high-risk group and a low-risk group. The high-risk group showed a higher mortality rate and lower survival rate with AUC of 0.785, 0.752, 0.756, 0.774 and 0.759 for the 1-, 2-, 3-, 4-, and 5-year respectively. A nomogram based on risk score, stage, T, and M had a better prognostic accuracy, with AUC of 0.808, 0.796, 0.811, 0.824 and 0.795 for the 1-, 2-, 3-, 4-, and 5-year respectively. The high-risk group showed enrichment in cell cycle, cell division, and chromosome processes, and a significantly higher tumor mutation burden (TMB) value compared to the low-risk group. Further immune infiltration analysis indicated a significantly reduction in the abundances of expression levels of some immune checkpoint (CD80, CTLA4, HAVCR2, and TNFRSF4) in the high-risk group. Moreover, the risk score was associated with the response to immune checkpoint inhibitors (ICIs) therapy and efficiencies of multiple chemotherapy drugs. Conclusions: This study developed a prognostic model based on MCMGs, which can predict the prognosis of liver cancer patients and their response to immunotherapy and chemotherapy. The model may provide new strategies to enhance the prognosis and treatment of HCC.

HIV remains a global health challenge, and research efforts directed towards a functional cure require people living with HIV (PLHIV) involvement in clinical trials. Our study assessed willingness to participate in HIV functional cure-related clinical trials and associated factors among PLHIV in Guangzhou, China, using a questionnaire survey approach. We analyzed responses from 718 questionnaires, finding that 71.2% were willing to participate in Phase III trials, while 51.7% were willing to participate in Phase I trials and 42.9% expressed acceptability for analytic treatment interruption. Multivariate logistic regression demonstrated that male PLHIV, those with awareness of functional cure, and PLHIV, who had been on antiretroviral therapy (ART) for less than 1 year, were more willing to participate in Phase III trials. Those with a body mass index greater than 24, and those without resistance to ART drug were more willing to participate in Phase I trials. The major motivations for participation in Phase III trials were access to cutting-edge treatments (62.6%) and supporting research (55.3%). Safety was the main concern contributing to hesitancy. Our study revealed a high willingness to participate in HIV functional cure-related trials among PLHIV in Guangzhou, China, and willingness varied across different trial phases and was influenced by multiple factors. This study provides valuable references for future clinical trial recruitment strategies and public health policy formulation.

With the development of modern communication and military technology, the protection of electromagnetic wave pollution and radar stealth technology have been widely concerned. In this background, the magnetic metals were widely used due to the strong ferromagnetism, which can induce strong magnetic loss to the electromagnetic wave. However, it remains challenging to precisely control the wave-absorbing performance of individual magnetic metals, and there are issues with poor corrosion resistance and high temperature resistance. Therefore, the alloy powders based on Fe, Co, and Ni in this work were prepared by wet chemical method at room temperature. Different from most of the traditional powder metallurgy methods, the wet chemical method enables us to obtain binary, ternary and quaternary alloys with a nanometer-scale and uniform distribution, all products have a microscopic morphology of 300-500nm spheres. The FeCoNi alloy powder shows an effective absorption band (RL<-10dB) of 7GHz in the high frequency electromagnetic wave range at an ultrathin thickness of 1.5mm, which has a high temperature magnetism (Tc >900K) and excellent corrosion resistance. Further doping of Cu to form a quaternary alloy can improve the corrosion resistance and maintain the original wave-absorbing properties, while doping of Mo and Sn can adjust the position of the effective absorption bandwidth.

BackgroundIsoniazid-resistant, rifampicin-susceptible tuberculosis (Hr-TB) globally exhibits a high prevalence and serves as a potential precursor to multidrug-resistant tuberculosis (MDR-TB). Recognizing the spatial distribution of Hr-TB and identifying associated factors can provide strategic entry points for interventions aimed at early detection of Hr-TB and prevention of its progression to MDR-TB. This study aims to analyze spatial patterns and identify socioeconomic, demographic, and healthcare factors associated with Hr-TB in Shanghai at the county level.MethodWe conducted a retrospective study utilizing data from TB patients with available Drug Susceptible Test (DST) results in Shanghai from 2010 to 2016. Spatial autocorrelation was explored using Global Moran’s I and Getis-Ord Gi∗ statistics. A Bayesian hierarchical model with spatial effects was developed using the INLA package in R software to identify potential factors associated with Hr-TB at the county level.ResultsA total of 8,865 TB patients with DST were included in this analysis. Among 758 Hr-TB patients, 622 (82.06%) were new cases without any previous treatment history. The drug-resistant rate of Hr-TB among new TB cases in Shanghai stood at 7.20% (622/8014), while for previously treated cases, the rate was 15.98% (136/851). Hotspot areas of Hr-TB were predominantly situated in southwestern Shanghai. Factors positively associated with Hr-TB included the percentage of older adult individuals (RR = 3.93, 95% Crl:1.93–8.03), the percentage of internal migrants (RR = 1.35, 95% Crl:1.15–1.35), and the number of healthcare institutions per 100 population (RR = 1.17, 95% Crl:1.02–1.34).ConclusionWe observed a spatial heterogeneity of Hr-TB in Shanghai, with hotspots in the Songjiang and Minhang districts. Based on the results of the models, the internal migrant population and older adult individuals in Shanghai may be contributing factors to the emergence of areas with high Hr-TB notification rates. Given these insights, we advocate for targeted interventions, especially in identified high-risk hotspots and high-risk areas.

Sm2Co17 permanent magnets are widely used in aerospace, radar communication, rail transit, and various other fields. However, their brittleness restricts their application. Improving their mechanical properties is of practical interest. Cryogenic treatment can be used as a means to enhance the mechanical properties of Sm2Co17 permanent magnets. However, the specific relationship between cryogenic treatment time and the properties of samarium-cobalt magnets has not yet been fully understood. In light of this, this paper focuses on exploring the influence of cryogenic treatment time on the mechanical properties of Sm2Co17 permanent magnets, aiming to provide a foundation for further elucidating this relationship. The results show that the mechanical properties can be significantly affected by cryogenic treatment time, with the flexural strength and elastic modulus increasing by 27.6 % and 9.6 % respectively when 3 h of treatment. However, the effect of further extending the treatment time to 6 h is not significant. In addition, the cryogenic treatment time has no adverse effect on magnetic properties. Additional analysis reveals that the enhancement in mechanical properties is primarily attributed to the decrease in residual stress and lattice constants. This study provides valuable insights into optimizing cryogenic treatment parameters to enhance the mechanical performance of Sm2Co17 permanent magnets while preserving their magnetic properties.

Download This Paper Open PDF in Browser Add Paper to My Library Share: Permalink Using these links will ensure access to this page indefinitely Copy URL Rna-Seq Reveals Transcriptome Changes in Fetal Head Following C-Flip Knockdown 14 Pages Posted: 27 Feb 2024 See all articles by Ting XieTing XieSun Yat-sen University (SYSU)Qiqi ChenSun Yat-sen University (SYSU)Nina LiSun Yat-sen University (SYSU)Shengze ZhangSun Yat-sen University (SYSU)Lin ZhuSun Yat-sen University (SYSU)Shaohui BaiSun Yat-sen University (SYSU)Haolu ZhaSun Yat-sen University (SYSU)Weijian TianSun Yat-sen University (SYSU)Chuming LuoSun Yat-sen University (SYSU)Nan WuShenzhen Center for Disease Control and PreventionXuan ZouShenzhen Center for Disease Control and PreventionShisong FangShenzhen Center for Disease Control and PreventionYuelong ShuSun Yat-sen University (SYSU) - School of Public HealthJianhui YuanShenzhen Center for Disease Control and PreventionYing JiangShenzhen Center for Disease Control and PreventionHuanle LuoSun Yat-sen University (SYSU) Abstract The FADD-like interleukin-1β converting enzyme (FLICE)-inhibitory protein (c-FLIP) plays a crucial role in various biological processes, including apoptosis and inflammation. However, the complete transcriptional profile altered by c-FLIP is not fully understood. Additionally, the impact of c-FLIP deficiency on the transcriptome during Zika virus (ZIKV) infection has not been elucidated. In this study, we compared transcriptome profiles between WT and c-Flip+/- fetal heads at embryonic day 13.5 from control and PBS-infected WT dams mated with c-Flip+/- sires. In the non-infected group, we observed differentially expressed genes (DEGs) mainly involved in embryonic development and neuron development. However, ZIKV infection significantly altered the transcriptional profile between WT and c-Flip+/- fetal heads. DEGs in pattern recognition receptor (PRR)-related signaling pathways, such as the RIG-I-like receptor signaling pathway and Toll-like receptor signaling pathway, were enriched. Moreover, the DEGs were also enriched in T cells, indicating that c-FLIP participates in both innate and adaptive immune responses upon viral infection. Furthermore, our observations indicate that DEGs are associated with sensory organ development and eye development, suggesting a potential role for c-FLIP in ZIKV-induced organ development defects. Overall, we have provided a comprehensive transcriptional profile for c-FLIP and its modulation during ZIKV infection. Note: Funding declaration: This work was supported by National Natural Science Foundation of China (32000116, 32270147), High-Level Project of Medicine in Nanshan, Shenzhen; Sanming Project of Medicine in Shenzhen (SZSM202103008), Science and Technology Planning Project of Guangdong Province in China (2021B1212040017), Shenzhen Science Technology Program (KQTD20200820145822023, JCYJ20190807155407443, JSGG20200225152008136, 20200825113322001, KCXFZ20211020172545006) and National Key Research and Development Program of China (2021YFC2300104). Conflict of Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Ethical Approval: The protocols for animal studies were approved by the Institutional Animal Care and Use Committee of Sun Yat-Sen University. Keywords: c-Flip, knockdown, fetal head, RNA Seq Suggested Citation: Suggested Citation Xie, Ting and Chen, Qiqi and Li, Nina and Zhang, Shengze and Zhu, Lin and Bai, Shaohui and Zha, Haolu and Tian, Weijian and Luo, Chuming and Wu, Nan and Zou, Xuan and Fang, Shisong and Shu, Yuelong and Yuan, Jianhui and Jiang, Ying and Luo, Huanle, Rna-Seq Reveals Transcriptome Changes in Fetal Head Following C-Flip Knockdown. Available at SSRN: https://ssrn.com/abstract=4733473 Ting Xie Sun Yat-sen University (SYSU) ( email ) Qiqi Chen Sun Yat-sen University (SYSU) ( email ) Nina Li Sun Yat-sen University (SYSU) ( email ) Shengze Zhang Sun Yat-sen University (SYSU) ( email ) Lin Zhu Sun Yat-sen University (SYSU) ( email ) Shaohui Bai Sun Yat-sen University (SYSU) ( email ) Haolu Zha Sun Yat-sen University (SYSU) ( email ) Weijian Tian Sun Yat-sen University (SYSU) ( email ) Chuming Luo Sun Yat-sen University (SYSU) ( email ) Nan Wu Shenzhen Center for Disease Control and Prevention ( email ) Xuan Zou Shenzhen Center for Disease Control and Prevention ( email ) Shenzhen, 518055China Shisong Fang Shenzhen Center for Disease Control and Prevention ( email ) Shenzhen, 518055China Yuelong Shu Sun Yat-sen University (SYSU) - School of Public Health ( email ) China Jianhui Yuan Shenzhen Center for Disease Control and Prevention ( email ) Shenzhen, 518055China Ying Jiang Shenzhen Center for Disease Control and Prevention ( email ) Huanle Luo (Contact Author) Sun Yat-sen University (SYSU) ( email ) Download This Paper Open PDF in Browser Do you have negative results from your research you’d like to share? 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